scholarly journals Synergistic Cytotoxicity Effect by Combination of Methanol Extract of Parijoto Fruit (Medinilla speciosa Reinw. ex. Bl) and Cisplatin Against Hela Cell Line

2020 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Anif Nur Artanti ◽  
Umi Hanik Pujiastuti ◽  
Fea Prihapsara ◽  
Rita Rakhmawati
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
Hela Cell Line ◽  
Agent Based ◽  
Chemotherapy Agent ◽  
Ic50 Value ◽  
Synergistic Cytotoxicity ◽  
Combinational Treatment

As one of the leading causes of death in worldwide, cervical cancer requires the effective therapies to reduce its mortality rate. One of the chemotherapy agents that frequently used in the treatment is cisplatin. However, due to drug resistance and its side effects, an agent that can be combined with cisplatin is needed. Parijoto fruit (Medinilla speciosa Reinw.ex.Bl) contains secondary metabolites compounds that have potential as anticancer. The study aims to determine the cytotoxic effect of methanol extract of Parijoto fruit calculated from the IC50 value and the synergicity of the combinational treatment with cisplatin evaluated from the Combination Index (CI) value and its cell viability by using MTT assay. Results showed that methanol extract of Parijoto fruit (MEP) performed cytotoxic effect on HeLa cell line with IC50 of 209.6 μg/mL while the value of IC50 of cisplatin against HeLa cells amounted to 12.8 μg/mL. The combination of 26.205 ppm (1/8 IC50) of MEP and 1.601 ppm (1/8 IC50) of Cisplatin performed synergistic effect on HeLa cell line with the CI value of 0.69. From the above results, it can be concluded that MEP is potential as co-chemotherapy agent based on the synergistic cytotoxicity effect with cisplatin.Keyword: cytotoxic, Medinilla speciosa, cisplatin, co-chemotherapy, MTT

scholarly journals IN-VITRO CYTOTOXICITY ACTIVITY OF MALAXIS RHEEDEI SW METHANOL EXTRACT AGAINST HELA CELL LINE AND MCF- 7 CELL LINE.

2016 ◽  
Vol 9 (6) ◽  
pp. 244 ◽  
Author(s):  
Renjini Haridas
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Mtt Assay ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Plant Part ◽  
Hela Cell Line ◽  
Cytotoxicity Activity ◽  
Whole Plant ◽  
Ic50 Value

Cancer is a group of diseases caused by loss of cell cycle control. Cancer is associated with abnormal uncontrolled cell growth. The study was aimed to evaluation of the anticancer activity of the Malaxis rheedei Sw. on the HeLa cell line and MCF- 7cell line. The whole plant part of the Malaxis rheedei methanolic extract were tested for its inhibitory effect on HeLa Cell Line and MCF- 7cell line. The cytotoxicity of Malaxis rheedei on HeLa cell and MCF- 7cell line were evaluated by the MTT assay. Malaxis rheedei methanolic extract has significant cytotoxicity effect on MCF- 7cell line in concentration range between 18.75 to 300 µg/ml by using MTT assay and study also showed that inhibitory action on HeLa cell line inconcentration range between 18.75 to 300 µg/ml by using MTT assay. Methanol extract of  the whole plant part of Malaxis rheedei was found to be 7.3%, 16.6%,  25.4%, 36.3% and 47.1%toxic in HeLa cell line and 7.9%, 13.9%, 26%, 48.4% and 66.3% toxic in MCF- 7cell line. IC50 value of Malaxis rheedei on MCF- 7cell was  167.76  µg/ml and IC50 value of Malaxis rheedei on HeLa Cell was not found by MTT assay. From the performed assay, methanol extract of these drug shows greater activity on MCF- 7cell line and little activity on HeLa cell line and that mean Malaxis rheedei can be used as anticancer activity.Keywords: Cytotoxicity Activity, MTT Assay, Malaxis rheedei Sw. , HeLa CellLine, MCF- 7Cell Line.

scholarly journals IN VITRO CYTOTOXICITY POTENTIAL OF ETHANOL EXTRACT OF SYZYGIUM SAMARANGENSE (Wt.)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-32
Author(s):  
Poonkodi K ◽  
Mini R ◽  
Vimaladevi K ◽  
Prabhu V ◽  
Anusuya M ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Hela Cell ◽  
Cell Line ◽  
Ethanol Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Line ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dependent Activity

The present investigation is carried out to study the invitro cytotoxicity of ethanol extract of Syzygium samarangense leaves on HeLa cell line by using MTT assay. Ethanol extract of S. samarangense showed concentration dependent activity on HeLa cell line with IC50 value of 40.5 μg/ml which shows that ethanol extract of S. samarangense posses significant cytoxicity.Moreover the preliminary phytochemical screening showed the presence of fatty acids, alkaloids, flavonoids, terphenoids, saponins, tannins and steroids which are responsible for its cytotoxicity. There are only a few reports are available for cytotoxicity of ethanol extract of S. samarangense.

scholarly journals Anticancer Evaluation of Plants from Indonesian Tropical Rain Forests

2011 ◽  
Vol 2 (3) ◽  
pp. 310
Author(s):  
Jamilah Abbas
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Line ◽  
Stem Bark ◽  
Tropical Rainforests ◽  
Hela Cell Line ◽  
Tropical Rain Forests ◽  
Rain Forests ◽  
Anticancer Activities ◽  
Ic50 Value

The anticancer activities of medicinal plants from Indonesia’s tropical rainforests were investigated against Hela cell line. Maytenfoliol from leaves of Calophyllumtetrapterum 3-epibetulinic acid from stem bark of C. tomentosum Wight and D.A-friedo-oleanan-3-on from stem bark of C. moonii showed anticancer activity. The compound 3-epibetulinic acid showed the more potent anticancer activity than maytenfoliol and D.A-friedo-oleanan-3- with thean IC50 value were 3.17 μg/mL, 4.89 μg/mL and 5.63 μg/mL, respectively.Keywords: Anticancer evaluation, Hela cell line, Calophyllumtetrapterum. C. tomentosum Wight and C. moonii

scholarly journals Synergistic Cytotoxic Effect from Combination of Wedelolactone and Cisplatin in HeLa Cell Line: A Novel Finding

2020 ◽  
Vol Volume 14 ◽  
pp. 3841-3852
Author(s):  
Sadia Sarwar ◽  
Jun Qing Yu ◽  
Humaira Nadeem ◽  
Fazlul Huq
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Hela Cell Line ◽  
Synergistic Cytotoxic Effect

scholarly journals Cytotoxic Activity of Pentacyclic Triterpene-3-Heptadecanoate Ester against Hela Cell Line and Its Docking Study

2017 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Sri Mulyani Mulyadi ◽  
Tri Yuliati ◽  
Maulana Tegar ◽  
Imam Ardhila
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Docking Study ◽  
Heptadecanoic Acid ◽  
Hela Cell Line ◽  
Activity Assay ◽  
Pentacyclic Triterpene ◽  
Acetate Esters ◽  
Mtt Method ◽  
Ic50 Value

Pentacyclic triterpene-3-heptadecanoate (12,13-dihydro-α-amyrin-20,30-en-3-heptadecanoate)ester refers to the compound as a result of reaction between pentacyclic triterpene-3-ol(12,13-dihydro-α-amyrin-20,30-en-3-ol) and heptadecanoic acid. This research was aimed to conductcytotoxicity test of pentacyclic triterpene-3-heptadecanoate; 12,13-dihydro-α-amyrin-20,30-en-3-oland 12,13-dihydro-α-amyrin-20,30-en-3-acetate esters against Hela cell line. The activity assay wascarried out using MTT method. The results indicated that IC50 value of 12,13-dihydro-α-amyrin-3-heptadecanoate; 12,13-dihydro-α-amyrin-20,30-en-3-acetate and 12,13-dihydro-α-amyrin-20,30-en-3-ol were 111.0, 151.1 and 944.4 μg/ml, respectively. The compound of 12,13-dihydro-α-amyrin-20,30-en-3-heptadecanoate had the lowest IC50 value, suggesting it has a potency to be synthesized as ananticancer drug.

scholarly journals AKTIVITAS SITOTOKSIK EKSTRAK ETANOLIK HERBA CIPLUKAN (Physalis angulata L.) PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI EKSPRESI PROTEIN p53

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Andita Pra Darma ◽  
Rosana Anna Ashari ◽  
Perdana Adhi Nugroho ◽  
Ameilinda Monikawati ◽  
Ilham Agusta Fauzi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Hela Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Ethanolic Extract ◽  
Cytotoxic Agents ◽  
Hela Cell Line ◽  
Potential Activity ◽  
Physalis Angulata

Cervical cancer is one of leading cause of cancer death in women in the developing countries. One of the strategy to prevent cervical cancer based on cytotoxic agents are now being developed. Ciplukan (Physalis angulata L.) is one of potential plant as chemopreventive agent due to Physalin and Withangulatin constituent in this plant. This study was aimed to know cytotoxic effect  of ciplukan ethanolic extract (CEE) in human cervical carcinoma Hela cell line. Evaluation of cell viability value was determined using MTT assay. The expression of p53 as cell proliferation regulator was observed with immunocytochemistry assay. Ethanolic extract of ciplukan showed cytotoxic effect in HeLa cell line with IC50 of 158 µg/ml. Further observation of cell proliferation regulator showed that CEE induces expression of p53 that inhibit cell proliferation. The result showed that CEE has potential activity to be developed as anticancer agent in human cervical cancer.

scholarly journals Antiproliferation potential of Botto-botto (Chromolaena odorata L.) leaves methanol extract fraction against HeLa Cell Line

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Muhammad Rusdi ◽  
Haeria Haeria ◽  
Nursalam Hamzah ◽  
Afrisusnawati Rauf ◽  
Faradibha Amriani
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Methanol Extract ◽  
Hela Cell Line ◽  
Chromolaena Odorata ◽  
Extract Fraction

Identification and Expression Analysis of CD73 Inhibitors in Cervical Cancer

2020 ◽  
Vol 16 ◽  
Author(s):  
Jamshed Iqbal ◽  
Ayesha Basharat ◽  
Sehrish Bano ◽  
Syed Mobasher Ali Abid ◽  
Julie Pelletier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Western Blot ◽  
Hela Cell ◽  
Cell Line ◽  
Cell Apoptosis ◽  
Immunofluorescence Staining ◽  
Cell Cycle Analysis ◽  
Hela Cell Line ◽  
Cell Line Hela

Aims: The present study was conducted to examine the inhibitory effects of synthesized sulfonylhydrazones on the expression of CD73 (ecto-5′-NT). Background: CD73 (ecto-5′-NT) represents the most significant class of ecto-nucleotidases which are mainly responsible for dephosphorylation of adenosine monophosphate to adenosine. Inhibition of CD73 played an important role in the treatment of cancer, autoimmune disorders, precancerous syndromes, and some other diseases associated with CD73 activity. Objective: Keeping in view the significance of CD73 inhibitor in the treatment of cervical cancer, a series of sulfonylhydrazones (3a-3i) derivatives synthesized from 3-formylchromones were evaluated. Methods: All sulfonylhydrazones (3a-3i) were evaluated for their inhibitory activity towards CD73 (ecto-5′-NT) by the malachite green assay and their cytotoxic effect was investigated on HeLa cell line using MTT assay. Secondly, most potent compound was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis. After that, CD73 mRNA and protein expression were analyzed by real-time PCR and Western blot. Results: Among all compounds, 3h, 3e, 3b, and 3c were found the most active against rat-ecto-5′-NT (CD73) enzyme with IC50 (µM) values of 0.70 ± 0.06 µM, 0.87 ± 0.05 µM, 0.39 ± 0.02 µM and 0.33 ± 0.03 µM, respectively. These derivatives were further evaluated for their cytotoxic potential against cancer cell line (HeLa). Compound 3h and 3c showed the cytotoxicity at IC50 value of 30.20 ± 3.11 µM and 86.02 ± 7.11 µM, respectively. Furthermore, compound 3h was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis which showed promising apoptotic effect in HeLa cells. Additionally, compound 3h was further investigated for its effect on expression of CD73 using qRT-PCR and western blot. Conclusion: Among all synthesized compounds (3a-3i), Compound 3h (E)-N'-((6-ethyl-4-oxo-4H-chromen-3-yl) methylene)-4-methylbenzenesulfonohydrazide was identified as most potent compound. Additional expression studies conducted on HeLa cell line proved that this compound successfully decreased the expression level of CD73 and thus inhibiting the growth and proliferation of cancer cells.

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