scholarly journals MTT Assay of Cissus Quadrangularison Hela Cell Line

2019 ◽  
Vol 8 (2S8) ◽  
pp. 1715-1718
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Mtt Assay ◽  
Hela Cell Line ◽  
Indigenous Plants

Approval of the helpful restorative properties of different indigenous plants has increased monstrous significance as they can be utilized as an elective wellspring of lead mixes in pharmacological ventures to handle numerous advanced issues, for example, tranquilize obstruction in microorganisms and nonparticularity in chemotherapeutic operators. Cissusquadrangularis Linn.is one such plant which has been generally utilized for therapeutic purposes in customary frameworks of prescription. Thus this work looks to approve the anticancer property of methanolic and ethanolic concentrates of the plant against HeLa cell line. The IC50 fixation was recorded at 62.5 ug/ml for methanolic separate and 125ug/ml for ethanolic remove. The outcomes unmistakably uncovered that the methanolic concentrate of Cissusquadrangularis was progressively powerful on HeLa cell line when contrasted with ethanolic extricate.

scholarly journals IN-VITRO CYTOTOXICITY ACTIVITY OF MALAXIS RHEEDEI SW METHANOL EXTRACT AGAINST HELA CELL LINE AND MCF- 7 CELL LINE.

2016 ◽  
Vol 9 (6) ◽  
pp. 244 ◽  
Author(s):  
Renjini Haridas
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Mtt Assay ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Plant Part ◽  
Hela Cell Line ◽  
Cytotoxicity Activity ◽  
Whole Plant ◽  
Ic50 Value

Cancer is a group of diseases caused by loss of cell cycle control. Cancer is associated with abnormal uncontrolled cell growth. The study was aimed to evaluation of the anticancer activity of the Malaxis rheedei Sw. on the HeLa cell line and MCF- 7cell line. The whole plant part of the Malaxis rheedei methanolic extract were tested for its inhibitory effect on HeLa Cell Line and MCF- 7cell line. The cytotoxicity of Malaxis rheedei on HeLa cell and MCF- 7cell line were evaluated by the MTT assay. Malaxis rheedei methanolic extract has significant cytotoxicity effect on MCF- 7cell line in concentration range between 18.75 to 300 µg/ml by using MTT assay and study also showed that inhibitory action on HeLa cell line inconcentration range between 18.75 to 300 µg/ml by using MTT assay. Methanol extract of  the whole plant part of Malaxis rheedei was found to be 7.3%, 16.6%,  25.4%, 36.3% and 47.1%toxic in HeLa cell line and 7.9%, 13.9%, 26%, 48.4% and 66.3% toxic in MCF- 7cell line. IC50 value of Malaxis rheedei on MCF- 7cell was  167.76  µg/ml and IC50 value of Malaxis rheedei on HeLa Cell was not found by MTT assay. From the performed assay, methanol extract of these drug shows greater activity on MCF- 7cell line and little activity on HeLa cell line and that mean Malaxis rheedei can be used as anticancer activity.Keywords: Cytotoxicity Activity, MTT Assay, Malaxis rheedei Sw. , HeLa CellLine, MCF- 7Cell Line.

Identification and Expression Analysis of CD73 Inhibitors in Cervical Cancer

2020 ◽  
Vol 16 ◽  
Author(s):  
Jamshed Iqbal ◽  
Ayesha Basharat ◽  
Sehrish Bano ◽  
Syed Mobasher Ali Abid ◽  
Julie Pelletier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Western Blot ◽  
Hela Cell ◽  
Cell Line ◽  
Cell Apoptosis ◽  
Immunofluorescence Staining ◽  
Cell Cycle Analysis ◽  
Hela Cell Line ◽  
Cell Line Hela

Aims: The present study was conducted to examine the inhibitory effects of synthesized sulfonylhydrazones on the expression of CD73 (ecto-5′-NT). Background: CD73 (ecto-5′-NT) represents the most significant class of ecto-nucleotidases which are mainly responsible for dephosphorylation of adenosine monophosphate to adenosine. Inhibition of CD73 played an important role in the treatment of cancer, autoimmune disorders, precancerous syndromes, and some other diseases associated with CD73 activity. Objective: Keeping in view the significance of CD73 inhibitor in the treatment of cervical cancer, a series of sulfonylhydrazones (3a-3i) derivatives synthesized from 3-formylchromones were evaluated. Methods: All sulfonylhydrazones (3a-3i) were evaluated for their inhibitory activity towards CD73 (ecto-5′-NT) by the malachite green assay and their cytotoxic effect was investigated on HeLa cell line using MTT assay. Secondly, most potent compound was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis. After that, CD73 mRNA and protein expression were analyzed by real-time PCR and Western blot. Results: Among all compounds, 3h, 3e, 3b, and 3c were found the most active against rat-ecto-5′-NT (CD73) enzyme with IC50 (µM) values of 0.70 ± 0.06 µM, 0.87 ± 0.05 µM, 0.39 ± 0.02 µM and 0.33 ± 0.03 µM, respectively. These derivatives were further evaluated for their cytotoxic potential against cancer cell line (HeLa). Compound 3h and 3c showed the cytotoxicity at IC50 value of 30.20 ± 3.11 µM and 86.02 ± 7.11 µM, respectively. Furthermore, compound 3h was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis which showed promising apoptotic effect in HeLa cells. Additionally, compound 3h was further investigated for its effect on expression of CD73 using qRT-PCR and western blot. Conclusion: Among all synthesized compounds (3a-3i), Compound 3h (E)-N'-((6-ethyl-4-oxo-4H-chromen-3-yl) methylene)-4-methylbenzenesulfonohydrazide was identified as most potent compound. Additional expression studies conducted on HeLa cell line proved that this compound successfully decreased the expression level of CD73 and thus inhibiting the growth and proliferation of cancer cells.

Molecular events after antisense inhibition of hMSH2 in a HeLa cell line

1998 ◽  
Vol 418 (2-3) ◽  
pp. 61-71 ◽  
Author(s):  
Ying Qian ◽  
Yingnian Yu ◽  
Xingruo Cheng ◽  
Jianhong Luo ◽  
Haiyang Xie ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Antisense Inhibition ◽  
Hela Cell Line ◽  
Molecular Events

scholarly journals Synthesis, structural analysis, solution equilibria and biological activity of rhodium(iii) complexes with a quinquedentate polyaminopolycarboxylate

RSC Advances ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 5282-5296 ◽  
Author(s):  
Marija S. Jeremić ◽  
Hubert Wadepohl ◽  
Vesna V. Kojić ◽  
Dimitar S. Jakimov ◽  
Ratomir Jelić ◽  
...  
Keyword(s):  
Biological Activity ◽  
Structural Analysis ◽  
Antitumor Activity ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cell Line ◽  
Solution Equilibria ◽  
Line P

Two new Rh(iii)–ed3a complexes [Rh(ed3a)(OH2)]·H2O and Na[Rh(ed3a)Cl]·H2O have shown good antitumor activity, especially against HeLa cell line.

Exploring the potential of imines as antiprotozoan agents with focus on t. Brucei and p. Falciparum

2018 ◽  
Author(s):  
◽  
Kola Augustus Oluwafemi
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Special Focus ◽  
Hela Cell Line ◽  
Free Energies ◽  
Imino Group ◽  
Design Synthesis ◽  
Lead Compounds ◽  
Pyridine Moiety ◽  
Nmr Study

This work focuses on the design, synthesis and evaluation of imine-containing heterocyclic and acyclic compounds with special focus on their bioactivity against parasitic protozoans (P. falciparum and T. brucei) - given the context of drug resistance in the treatment of malaria and Human African sleeping sickness and the fact that several bioactive organic compounds have been reported to possess the imino group. Starting from 2-aminopyridine, novel #-alkylated-5-bromo-7-azabenzimidazoles and substituted 5-bromo-1-(carbamoylmethy)-7-azabenzimidazole derivatives were prepared, and their bioactivity against parasitic protozoans was assessed. NMR spectra of the substituted 5- bromo-1-(carbamoylmethy)-7-azabenzimidazole derivatives exhibited rotational isomerism, and a dynamic NMR study was used in the estimation of the rate constants and the free- energies of activation for rotation. The free-energy differences between the two rotamers were determined and the more stable conformations were predicted. Novel 2-phenyl-7-azabenzimidazoles were also synthesised from 2-aminopyridine. A convenient method for the regioselective formylation of 2,3-diaminopyridines into 2-amino- 7-(benzylimino)pyridine analogues of 2-phenyl-7-azabenzimidazole was developed, and some of the resulting imino derivatives were hydrogenated to verify the importance of the imino moiety for bioactivity. The 2-phenyl-7-azabenzimidazoles and the 2-amino-7- (benzylimino)pyridine analogues were screened for their anti-protozoal activity and their cytotoxicity level was determined against the HeLa cell line. In order to validate the importance of the pyridine moiety, novel #-(phenyl)-2- hydroxybenzylimines, #-(benzyl)-2-hydroxybenzylimines and (±)-trans-1,2-bis[2- hydroxybenzylimino]cyclohexanes were also synthesized and screened for activity against the parasitic protozoans and for cytotoxicity against the HeLa cell line. The biological assay results indicated that these compounds are not significantly cytotoxic and a good number of them show potential as lead compounds for the development of new malaria and trypanosomiasis drugs.

331P Synergistic effects of ferula gummosa and radiotherapy to induce cytotoxicity and apoptosis in the hela cell line

2016 ◽  
Vol 27 (suppl_9) ◽  
Author(s):  
A. Fani-Pakdel ◽  
S.H. Forouzmand ◽  
S.H. Mousavi ◽  
V. Vazifedan ◽  
M. Nourbakhsh ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Synergistic Effects ◽  
Hela Cell Line
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