Comparative Analysis of Temperature on Yield of Different Varieties of Oyster Mushroom Production

Oyster mushrooms are economical and most easily grown of all cultivated edible mushrooms. The crop has a range of varieties, differing in form, colour, texture and odor, which can be cultivated throughout the year under a diverse agro-climatic conditions. Three different oyster mushroom species viz., Hypsizygus ulmarius (var. CO2), Pleurotus eous (var. APK1) and Pleurotus florida (var. PF) along with three cropping rooms of varied temperatures was used for the study. Among the different cropping rooms, thatched shed with a temperature of 23o C recorded a highest yield of 748g, 712 and 673 g per 500 g of substrate by PF, CO 2 and APK 1 respectively than AC room and Concrete room. The temperature of the cropping room is inversely proportional to the yield of oyster mushroom. Hence, the thatched shed was best suited for oyster mushroom cultivation, which was both economic and easy to use.

​Efficiency of Biocomposted Agroindustrial Wastes and Their Response in the Growth and Yield of Vigna unguiculata (L.) Walp

Background: Agroindustrial by-products of cocoa shell and jack fruit peels generally considered as organic waste have almost no economic value and create a big problem in disposal time. A significant amount of biocomposted cocoa shell and jack fruit peel waste is used as organic manure that enhances growth in Vigna unguiculata (L.) Walp. Methods: This research work was conducted in the period of 2019 in the Department of Botany, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu (India). A large amount of cocoa shell and jack fruit peel waste was used for the biocompost preparation using Pleurotus eous, Pleurotus florida spawns and Eudrilus eugeniae. After 90 days, biocompost were taken and sieved used as manure for plant study. Eight different treatments were carried out for the cultivation of Cowpea. Plant samples at various stages (25, 35 and 45 DAS) were analyzed for shoot and root length, number of leaves, flowers, nodules /plant, fresh weight and dry weight of plant. On 65th day yield parameters like number of pods/plant, length of the pod, number of seeds/pod, weight of seeds/pods, pod fresh weight and pod dry weight were analyzed. Result: The experimental results showed that the application T8 (Raw jackfruit peel + 10 g Pleurotus eous + 10 g Pleurotus florida + Eudrilus eugeniae 5 t/ha-1) recorded maximum root (20.63 cm, 32.73 cm and 39.23 cm), shoot length (70.73 cm, 167.50 cm and 175.83 cm), number of leaves/plant (26.46 cm, 33.53 cm and 37.50 cm), number of flowers/plant (12), number of nodules (14, 21.20 and 11.34), fresh weight (10.076 g, 12.146 g and 14.047 g) and dry weight (1.744 g, 1.854 g and 2.827 g) are closely followed by T4 (Raw cocoa shell+ 10 g Pleurotus eous + 10 g Pleurotus florida + Eudrilus eugeniae 5 t/ha-1). A significant increase in the yield parameters such as number of pods/plant (21), length of pod (16.50 cm), number of seeds/pod (20), weight of seed/pod (1.68 g), fresh weight (5.711 g) and dry weight of pod (2.398 g) was observed in treatment T8 as compared to the other treatments (T1, T2, T3, T5, T6 and T7) and control respectively.

Differentiation of Two Pleurotus Species Based on the Restrictive Digestion Profile of the Internal Transcribed Spacer Region

Two oyster mushrooms (Pleurotus eous P-31 and P. ostreatus EM-1) are under either cottage industry or semi-commercial cultivation in Ghana. The latter (P. ostreatus) is already well known to the public and on the shelf of some leading supermarkets. There is morphological resemblance between the two species making it difficult for the untrained eye to distinguish between them except for the colour difference. In this study, molecular methods were em­ployed to differentiate among the two species. The Internal Transcribed Spacer ITS 1 and ITS 4 regions of the rDNA of the two oyster species were amplified by the conventional PCR using the universal primer pair, ITS 1 and ITS 4 followed by restrictive digestion with enzymes, (Hh I, Hinf I, Rsa I and Hae III). The two species could not be separated based on the ampli­fied bands only, as both produced a characteristic band size of 650 bp. Gel profiling showing restrictive patterns generated by the four enzymes indicated that only the Hae III restrictive enzyme was effective in separating P. eous P-31 and P. ostreatus EM-1. This is the first record of the separation of the Ghanaian Pleurotus species by molecular methods indicating their genetic differences.

Invitro Cytotoxicity and Glucose Uptake Activity of Mushroom Pleurotus Eous in Methanol Extract Using L-6 Cell Line

The aim of the present study is to evaluate the cytotoxicity and glucose uptake activity of methanolic extract of pleurotus eous mushroom using L6 cell lines. An MTT assay is a colorimetric assay based on assessing the cell metabolic activityThe results showed that the extracts did not confer any cytotoxicity. The effects were observed in treated cells up to 6.25 mg/ml extract as measured with MTT. Also the mushroom extracts shows better glucose uptake potential. The results were compared with Insulin a standard antidiabetic drug. Insulin enhance the glucose uptake upto 97.8%. The L6 cell lines enhance the glucose uptake by 57.4% at 65µg concentration.

IN VITRO STUDIES ON ANTIOXIDANT AND ANTIDIABETIC ACTIVITY OF PLEUROTUS EOUS MUSHROOM IN METHANOL AND AQUEOUS EXTRACT

Objective: The present study evaluates the antioxidant and antidiabetic activity of the mushroom.Methods: Antioxidant activity was evaluated using hydroxyl radical, hydrogen peroxide, and antidiabetic activity using α-amylase and α-glucosidase.Result: The antioxidant IC50 for the mushroom extracts methanol and aqueous(Hydroxyl radical) was found to be 290,440μg/ml (Hydrogen peroxide) 475,370 μg/ml and antidiabetic(α Amylase) IC50 was found to be 460,500 μg/ml and (α Glucosidase) 325,280 μg/ml respectively.Conclusion: The result obtained in the in vitro methods suggests that Pleurotus eous mushroom can be administered for its antioxidant and antidiabetic activity