DEVELOPMENT AND CHARACTERIZATION OF BIOCOMPATIBLE POLYHYDROXY BUTYRATE IMPREGNATED WITH HERBAL PLANTS AGAINST WOUND HEALING ACTIVITY ON IN VIVO ANIMAL MODEL

Objective: The current study is to evaluate the antimicrobial, antioxidant, anti-inflammatory, and in vitro cytotoxicity activities of polyhydroxybutyrate (PHB) and to develop the herbal impregnated PHB cast film for wound healing activities using Albino Wistar rat model. Methods: PHB produced by Azotobacter chroococcum A3 strain was synthesized and characterized (previous study). The PHB was subjected to various biocompatibility studies such as antimicrobial, antioxidant, and anti-inflammatory studies. The PHB was also subjected to cytotoxicity study by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. PHB films were made using different combinations of plant and algal blends (herbal blends). The herbal blends of PHB films were evaluated for in vivo wound healing activity using Albino Wistar rats. Results: The turmeric impregnated PHB showed the highest result for antimicrobial with 27.25±0.23 mm against skin pathogens and antioxidant activity with the highest percentage of inhibition of 76%. The result predicts that PHB will not let to any toxic substances rather it acts as a chemoprotective agent followed by the inhibitory concentration value was found to be 1.56 μg/ml for 100 μg. The in vivo study showed better wound healing activity for PHB blended with 2% turmeric leaf and rhizome cast film. Whereas the wound healing activity of control and crude PHB was 90.4±0.4 and 91.3±0.56 respectively. Conclusion: The results from the present study showed that PHB can act as a good candidate for drug carrier and it is biocompatible in living cells.

In vitro Anti-inflammatory and In vivo Wound Healing Activity of Gardenia gummifera L.f Fruit and Stem bark Methanol Extracts in Rats

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2021.13.01.482 ◽

2021 ◽

Vol 13 (01) ◽

Keyword(s):

Wound Healing ◽

Stem Bark ◽

Methanol Extracts ◽

Anti Inflammatory ◽

Anti-inflammatory, analgesic and in vivo-in vitro wound healing potential of the Phlomis rigida Labill. extract

Journal of Ethnopharmacology ◽

10.1016/j.jep.2020.113408 ◽

2021 ◽

Vol 266 ◽

pp. 113408

Author(s):

Mehmet Evren Okur ◽

Ayşe Esra Karadağ ◽

Yağmur Özhan ◽

Hande Sipahi ◽

Şule Ayla ◽

...

Keyword(s):

Wound Healing ◽

Metabolite profiling and wound-healing activity of Boerhavia diffusa leaf extracts using in vitro and in vivo models

Journal of Traditional and Complementary Medicine ◽

10.1016/j.jtcme.2019.02.002 ◽

2020 ◽

Vol 10 (1) ◽

pp. 52-59 ◽

Cited By ~ 4

Author(s):

Kriti Juneja ◽

Rutusmita Mishra ◽

Samrat Chauhan ◽

Sumeet Gupta ◽

Partha Roy ◽

...

Keyword(s):

Wound Healing ◽

Metabolite Profiling ◽

Leaf Extracts ◽

In Vivo Models ◽

Boerhavia Diffusa ◽

Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

Applied Sciences ◽

10.3390/app10051845 ◽

2020 ◽

Vol 10 (5) ◽

pp. 1845 ◽

Cited By ~ 2

Author(s):

Alexandra M. Afonso ◽

Joana Gonçalves ◽

Ângelo Luís ◽

Eugenia Gallardo ◽

Ana Paula Duarte

Keyword(s):

Wound Healing ◽

Phenolic Compounds ◽

Protein Denaturation ◽

Radical Scavenging ◽

Total Phenolic ◽

Natural Substances ◽

Anti Inflammatory ◽

Free Radical Scavenging Assay ◽

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.

In Vitro and In Vivo Wound Healing and Anti-Inflammatory Activities of Babassu Oil (Attalea speciosa Mart. Ex Spreng., Arecaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/8858291 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

José Alex A. Santos ◽

José Wellinton da Silva ◽

Simone Maria dos Santos ◽

Maria de Fátima Rodrigues ◽

Camila Joyce A. Silva ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Palm Tree ◽

Amazon Forest ◽

Myeloperoxidase Activity ◽

Skin Wounds ◽

Babassu Oil ◽

Babassu (Attalea speciosa Mart. ex Spreng., Arecaceae) is a palm tree endemic to Brazil and found mainly in the borders of Amazon forest, where the harvesting of its fruits is an important source of income for more than 300,000 people. Among the communities of coconut breakers women, babassu oil is used in culinary, as fuel, and mostly as medicinal oil for the treatment of skin wounds and inflammation. This study aimed to evaluate in vitro and in vivo the wound healing effects of babassu oil. In vitro, babassu oil increased the migration of L929 fibroblasts, inhibited the production of nitric oxide by LPS-stimulated peritoneal macrophages, and increased the levels of INF-γ and IL-6 cytokines production. In vivo, babassu oil accelerated the healing process in a full-thickness splinted wound model, by an increase in the fibroblasts number, blood vessels, and collagen deposition in the wounds. The babassu oil also increased the recruitment of inflammatory cells into the wound site and showed an anti-inflammatory effect in a chronic ear edema model, reducing ear thickness, epidermal hyperplasia, and myeloperoxidase activity. Thus, these data corroborate the use of babassu oil in folk medicine as a remedy to treat skin wounds.

Wound Healing Activity of α-Pinene and α-Phellandrene

Molecules ◽

10.3390/molecules26092488 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2488

Author(s):

Judith Salas-Oropeza ◽

Manuel Jimenez-Estrada ◽

Armando Perez-Torres ◽

Andres Eliu Castell-Rodriguez ◽

Rodolfo Becerril-Millan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Folk Medicine ◽

Fibroblast Cell ◽

In Vitro Tests ◽

Low Concentrations ◽

In Vivo Tests ◽

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. Recently, it was shown that the essential oil (EO) of B. morelensis has wound healing activity, accelerating cutaneous wound closure and generating scars with good tensile strength. α-pinene (PIN) and α-phellandrene (FEL) are terpenes that have been found in this EO, and it has been shown in different studies that both have anti-inflammatory activity. The aim of this study was to determine the wound healing activity of these two terpenes. The results of in vitro tests demonstrate that PIN and FEL are not cytotoxic at low concentrations and that they do not stimulate fibroblast cell proliferation. In vivo tests showed that the terpenes produce stress-resistant scars and accelerate wound contraction, due to collagen deposition from the early stages, in wounds treated with both terpenes. Therefore, we conclude that both α-pinene and α-phellandrene promote the healing process; this confirms the healing activity of the EO of B. morelensis, since having these terpenes as part of its chemical composition explains part of its demonstrated activity.

Sida tuberculata: In vitro cytotoxicity and in vivo anti-inflammatory effect

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.114956 ◽

2021 ◽

pp. 114956

Author(s):

Hemerson Silva da Rosa ◽

Marí Castro Santos ◽

Marcio Tavares Costa ◽

Andréia Salgueiro ◽

Morgana Duarte da Silva ◽

...

Keyword(s):

In Vitro Cytotoxicity ◽

Anti Inflammatory ◽

Inflammatory Effect

Angiostatin is a novel anti-inflammatory factor by inhibiting leukocyte recruitment

Blood ◽

10.1182/blood-2004-01-0166 ◽

2005 ◽

Vol 105 (3) ◽

pp. 1036-1043 ◽

Cited By ~ 53

Author(s):

Triantafyllos Chavakis ◽

Athanasios Athanasopoulos ◽

Joong-Sup Rhee ◽

Valeria Orlova ◽

Thomas Schmidt-Wöll ◽

...

Keyword(s):

Wound Healing ◽

Vascular Injury ◽

Healing Process ◽

Specific Interaction ◽

Angiogenesis Inhibitor ◽

Terminal Phase ◽

Inflammatory Factor ◽

AbstractAngiogenesis and inflammation are closely related biologic processes in wound healing and the responses to vascular injury as well as in cardiovascular diseases; however, the molecular connections are poorly defined. In particular, it is yet unclear whether endogenous factors can regulate both angiogenesis and inflammation. Here, we show that the endogenous angiogenesis inhibitor, angiostatin (containing kringle domains 1-4 of plasminogen), serves an anti-inflammatory role, since the kringles 1-3 and its kringle 4 directly interact with leukocyte β1- and β2-integrins, respectively. In particular, a specific interaction between kringle 4 and αMβ2-integrin (Mac-1) but not leukocyte function antigen 1 (LFA-1) was identified. Angiostatin thereby inhibited β1- and β2-integrin–mediated adhesion of leukocytes to extracellular matrix proteins and the endothelium as well as their transmigration through the endothelium in vitro. Moreover, angiostatin blocked the peritonitis-induced neutrophil emigration in vivo. In addition, through its interaction with Mac-1, angiostatin reduced activation of the proinflammatory transcription factor nuclear factor κB (NFκB), as well as the NFκB-related expression of tissue factor, a potent initiator of hemostasis following vascular injury. Finally, angiostatin forms were generated in vivo following skin injury/inflammation and were detectable during the following entire period of wound healing peaking at the terminal phase of the healing process. Taken together, over and above inhibition of neovascularization, angiostatin was identified as an antiadhesive/anti-inflammatory substance. These observations could provide the basis for new therapeutic applications of angiostatin to target chronic inflammatory processes in different pathologic situations.

Nigella sativa and Its Active Compound, Thymoquinone, Accelerate Wound Healing in an In Vivo Animal Model: A Comprehensive Review

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17114160 ◽

2020 ◽

Vol 17 (11) ◽

pp. 4160 ◽

Cited By ~ 1

Author(s):

Nusaibah Sallehuddin ◽

Abid Nordin ◽

Ruszymah Bt Hj Idrus ◽

Mh Busra Fauzi

Keyword(s):

Wound Healing ◽

Nigella Sativa ◽

Healing Process ◽

Skin Wound ◽

Tissue Growth ◽

Inclusion Criteria ◽

Skin Wound Healing ◽

In Vivo Animal Model ◽

Nigella sativa (NS) has been reported to have a therapeutic effect towards skin wound healing via its anti-inflammatory, tissue growth stimulation, and antioxidative properties. This review examines all the available studies on the association of Nigella sativa (NS) and skin wound healing. The search was performed in Medline via EBSCOhost and Scopus databases to retrieve the related papers released between 1970 and March 2020. The principal inclusion criteria were original article issued in English that stated wound healing criteria of in vivo skin model with topically applied NS. The search discovered 10 related articles that fulfilled the required inclusion criteria. Studies included comprise different types of wounds, namely excisional, burn, and diabetic wounds. Seven studies unravelled positive results associated with NS on skin wound healing. Thymoquinone has anti-inflammatory, antioxidant, and antibacterial properties, which mainly contributed to wound healing process.

Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing

Polymers ◽

10.3390/polym12112619 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2619

Author(s):

Abegaz Tizazu Andrgie ◽

Haile Fentahun Darge ◽

Tefera Worku Mekonnen ◽

Yihenew Simegniew Birhan ◽

Endiries Yibru Hanurry ◽

...

Keyword(s):

Wound Healing ◽

Drug Carrier ◽

Saline Group ◽

Mice Model ◽

Thermosensitive Hydrogel ◽

Vitro Assay ◽

Raw264.7 Macrophages ◽

Moist Environment

Hydrogels have been investigated as ideal biomaterials for wound treatment owing to their ability to form a highly moist environment which accelerates cell migration and tissue regeneration for prompt wound healing. They can also be used as a drug carrier for local delivery, and are able to activate immune cells to enhance wound healing. Here, we developed heparin-conjugated poly(N-isopropylacrylamide), an injectable, in situ gel-forming polymer, and evaluated its use in wound healing. Ibuprofen was encapsulated into the hydrogel to help reduce pain and excessive inflammation during healing. In addition to in vitro studies, a BALB/c mice model was used to evaluate its effect on would healing and the secretion of inflammatory mediators. The in vitro assay confirmed that the ibuprofen released from the hydrogel dramatically reduced lipopolysaccharide-induced inflammation by suppressing the production of NO, PGE2 and TNF-α in RAW264.7 macrophages. Moreover, an in vivo wound healing assay was conducted by applying hydrogels to wounds on the backs of mice. The results showed that the ibuprofen-loaded hydrogel improved healing relative to the phosphate buffered saline group. This study indicates that ibuprofen loaded in an injectable hydrogel is a promising candidate for wound healing therapy.