scholarly journals IN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES

2020 ◽  
pp. 92-95
Author(s):  
ANITHA P ◽  
NAZEEMA TH
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Glutathione Reductase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Enzymic Antioxidants

Objectives: This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of Crescentia cujete. Methods: Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of C. cujete leaf. Results: The leaf extract of C. cujete showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of C. cujete leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid. Conclusion: From the results, it has concluded that the ethanol extract of the C. cujete leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.

scholarly journals Free Radical Scavenging Activity of Ethanolic Extract of Citrus paradisi and Naringin -An In vitro Study

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
R Roghini ◽  
K. Vijayalakshmi
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Citrus Paradisi

The present study attempts to find naturally-occurring antioxidants of fruit-based which give efficacy by additive activities. Citrus paradisi, known as Grape fruit contains significant bioactive components such as Naringin. The present study examines the free radical scavenging activity of ethanolic extract of Citrus paradisi and Naringin. The study was carried out with different radical scavenging assays like hydroxyl, DPPH, hydrogen peroxide, nitric oxide, super oxide. Citrus paradisi extracts showed lower radical scavenging activities in assays such as DPPH, superoxide and hydroxyl when compared with Naringin. Naringin showed the higher radical scavenging effect with nitric oxide, and hydrogen peroxide in comparison with citrus paradisi extract. However, both were analysed by using ascorbic acid as standard. The current study gives evidence that both showed potential free radical scavenging activity.

scholarly journals Assesment of free radical scavenging activity and inhibitory properties of aqueous extract of Cymodocea serrulata segrass towards α- glucosidase and α –amylase enzyme

2021 ◽  
Vol 23 (12) ◽  
pp. 339-360
Author(s):  
Suresh kumar Tirupur Venkatachalam ◽  
◽  
SenthilKumar Palanisamy ◽  
Sudhagar Babu ◽  
Ranjithkumar Rajamani ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Cymodocea Serrulata

Aim: The study reports the in vitro free radical scavenging and α-amylase and α-glucosidase inhibition activities of aqueous extract of seagrass Cymodocea serrulata from Gulf of Mannar, Southeast Asia. Methods: The free radical scavenging activity was evaluated by DPPH, Superoxide, Nitric oxide and Hydroxyl radical scavenging assays and α-amylase, α-glucosidase enzyme inhibitory activity. The C. serrulata showed effective DPPH radical scavenging activity of 69.45 μg/mL, Superoxide radical scavenging activity of 91.08.5 ± 0.02%, Nitric oxide radical scavenging activity of 86.89 ± 0.03% and Hydroxyl radical scavenging activity of 81.09 ± 0.3 % at a concentration of 5 μg/mL respectively. It also showed significant inhibitory activity against α- amylase with an IC50 value of 3.0 ± 0.04 (μg/ml) and the α-glucosidase inhibitory activity with an IC50 value of 3.7 ± 1.01 (μg/ml) at concentrations of 1-5 μg/mL. Conclusion: Cymodocea serrulata was found to be a potent free radical scavenger and inhibitor of α-amylase and α-glucosidase enzymes.

scholarly journals Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB.

Molekul ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 133 ◽  
Author(s):  
Waras Nurcholis ◽  
Nurul Khumaida ◽  
Muhamad Syukur ◽  
Maria Bintang
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Ic50 Values

This study evaluated the free radical scavenging activity in ethanolic extracts from 20 accessions of Curcuma aeruginosa. The radical scavenging activity of the extract accessions was investigated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. IC50 values for DPPH radical scavenging activity ranged from 89.81 to 505.65 µg mL-1. Based on IC50 values, twenty accessions of C. aeruginosa can be divided into three groups: strong (two accessions); moderate (seventeen accessions); and low (one accession) of DPPH scavenger. Sukoharjo (SH) and Muara Bungo (MB) showed promising accessions for antioxidant potential, thus these accessions important to selection for future breeding program in pharmaceutical products.

scholarly journals EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF MIMOSA PUDICA THORNS

2018 ◽  
Vol 11 (11) ◽  
pp. 153 ◽  
Author(s):  
Lakshmibai R ◽  
Amirtham D
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Mimosa Pudica ◽  
Age Related ◽  
Dpph Method

Objective: Mimosa pudica is a traditional medicinal plant. The natural antioxidant compounds from plants mop up the free radicals causing cell damage and maintain the biological systems. The aim of the present study is to evaluate the free radical scavenging potential of the ethanolic and aqueous thorn extracts of M. pudica.Methods: The ethanolic and aqueous thorn extracts of M. pudica were analyzed for the phytochemicals and for free radical scavenging activity by 1, 1 diphenyl 1-2-picric hydrazine (DPPH), 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and nitric oxide free radical scavenging method.Results: Flavonoids, saponins, glycosides, alkaloids, terpenoids, and coumarins were the phytoconstituents revealed in ethanolic and aqueous thorn extracts of M. pudica. At 250 μg/mL concentrations, aqueous thorn extracts of M. pudica exhibited 73.41% radical scavenging activity by DPPH method and 26.10% inhibition by nitric oxide free radical scavenging method. However, the ethanolic extracts of M. pudica thorns exhibited 73.35% inhibition by ABTS free radical scavenging method at 250 μg/mL concentrations.Conclusion: The results obtained suggest that the plant extracts from M. pudica could serve as a potential source of antioxidant in slowing down the process of aging and age-related or oxidative stress-related degenerative diseases. Moreover, the isolation of bioactive principle responsible for the antioxidant activity and formulation of novel therapeutic agents can be further studied.

IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ZIZIPHUS RUGOSA LAM. LEAVES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (07) ◽  
pp. 69-75
Author(s):  
S Parashar ◽  
V. Uplanchiwar ◽  
R. K. Gautam ◽  
S. Goyal ◽  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value

Ziziphus rugosa Lam. belongs to the family Rhamnaceae and is found chiefly in deciduous and semi evergreen forest of Western Ghats. The present research was undertaken to establish in vitro antioxidant and in vivo hepatoprotective activity of ethanolic extract of Z.rugosa Lam. leaves. The powdered leaves of Z. rugosa were extracted with ethanol and preliminary phytochemical screening was performed for the presence of various phytoconstituents. DPPH assay and β-glucuronidase inhibition assay were selected for the free radical scavenging activity. For the assessment of hepatoprotective activity, alcohol and CCl4 induced hepatotoxicity model were used. The phytochemical analysis of ethanolic extract showed the presence of alkaloids, saponins and flavonoids. The extract exhibited concentration dependent radical scavenging activity with an IC50 value of 61.88 μg/ml and β –glucoronidase inhibition activity with an IC50 value of 70.61 μg/ml. It was speculated that the Z. rugosa Lam. ethanolic extract shows dosedependent hepatoprotective activity which is equivalent with the standard drug Silymarin. The inhibition of free radicals or free radical scavenging activity is significant in the protection against CCl4 and alcohol induced hepatopathy. Hence, it is likely that the antioxidant activity of ethanolic extract of Z. rugosa Lam. might contribute to the hepatoprotective action.

scholarly journals Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L.

2011 ◽  
Vol 65 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Pavle Maskovic ◽  
Jelena Mladenovic ◽  
Milica Cvijovic ◽  
Gordana Acamovic-Djokovic ◽  
Slavica Solujic ◽  
...  
Keyword(s):  
Free Radical ◽  
Petroleum Ether ◽  
Hypericum Perforatum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dry Extract ◽  
Hypericum Perforatum L

The objective of this study was to evaluate antifungal and antioxidant activities of Hypericum perforatum L. extracts against the growth of certain fungi. The ethanolic, acetonic and petroleum ether extracts of the plant were evaluated for phenols, flavonoids and non-flavonoids. The highest amounts of phenols (17.6 mg EPC/g dry extract) and flavonoids (16.85 mg EPC/g dry extract) were found in the acetonic extract. The highest inhibitory effect on the growth of Penicillium canescens, Fusarium oxysporum, Alternaria alternata, Aspergillus glaucus and Phialophora fastigiata by the disk diffusion method was exhibited by the ethanolic extract at the concentration of 25 mg/disk. The minimum inhibitory concentration (MIC) of the ethanolic and petroleum ether extracts was 20 mg/mL. The acetonic extract did not affect the growth of the tested fungi. Antioxidant activity was assessed by determining 1,1-Diphenyl-2-picryl-hydrazyl-hydrate (DPPH) free radical scavenging activity. The results showed that the ethanolic extract of Hypericum perforatum L. possesses antioxidant activity. The IC50 values, defined as the concentration of the test sample leading to 50% reduction of the free radical concentration, determined for each measurement were <7.8125, 105.9, 5.99 and 12.77 ?g/ml for the ethanolic extract, the acetonic extract, ascorbic acid and BHT, respectively, for DPPH free radical scavenging activity.

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