scholarly journals Assesment of free radical scavenging activity and inhibitory properties of aqueous extract of Cymodocea serrulata segrass towards α- glucosidase and α –amylase enzyme

2021 ◽  
Vol 23 (12) ◽  
pp. 339-360
Author(s):  
Suresh kumar Tirupur Venkatachalam ◽  
◽  
SenthilKumar Palanisamy ◽  
Sudhagar Babu ◽  
Ranjithkumar Rajamani ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Cymodocea Serrulata

Aim: The study reports the in vitro free radical scavenging and α-amylase and α-glucosidase inhibition activities of aqueous extract of seagrass Cymodocea serrulata from Gulf of Mannar, Southeast Asia. Methods: The free radical scavenging activity was evaluated by DPPH, Superoxide, Nitric oxide and Hydroxyl radical scavenging assays and α-amylase, α-glucosidase enzyme inhibitory activity. The C. serrulata showed effective DPPH radical scavenging activity of 69.45 μg/mL, Superoxide radical scavenging activity of 91.08.5 ± 0.02%, Nitric oxide radical scavenging activity of 86.89 ± 0.03% and Hydroxyl radical scavenging activity of 81.09 ± 0.3 % at a concentration of 5 μg/mL respectively. It also showed significant inhibitory activity against α- amylase with an IC50 value of 3.0 ± 0.04 (μg/ml) and the α-glucosidase inhibitory activity with an IC50 value of 3.7 ± 1.01 (μg/ml) at concentrations of 1-5 μg/mL. Conclusion: Cymodocea serrulata was found to be a potent free radical scavenger and inhibitor of α-amylase and α-glucosidase enzymes.

scholarly journals IN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES

2020 ◽  
pp. 92-95
Author(s):  
ANITHA P ◽  
NAZEEMA TH
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Glutathione Reductase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Enzymic Antioxidants

Objectives: This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of Crescentia cujete. Methods: Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of C. cujete leaf. Results: The leaf extract of C. cujete showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of C. cujete leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid. Conclusion: From the results, it has concluded that the ethanol extract of the C. cujete leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.

scholarly journals Free Radical Scavenging Activity of Ethanolic Extract of Citrus paradisi and Naringin -An In vitro Study

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
R Roghini ◽  
K. Vijayalakshmi
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Citrus Paradisi

The present study attempts to find naturally-occurring antioxidants of fruit-based which give efficacy by additive activities. Citrus paradisi, known as Grape fruit contains significant bioactive components such as Naringin. The present study examines the free radical scavenging activity of ethanolic extract of Citrus paradisi and Naringin. The study was carried out with different radical scavenging assays like hydroxyl, DPPH, hydrogen peroxide, nitric oxide, super oxide. Citrus paradisi extracts showed lower radical scavenging activities in assays such as DPPH, superoxide and hydroxyl when compared with Naringin. Naringin showed the higher radical scavenging effect with nitric oxide, and hydrogen peroxide in comparison with citrus paradisi extract. However, both were analysed by using ascorbic acid as standard. The current study gives evidence that both showed potential free radical scavenging activity.

scholarly journals EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF MIMOSA PUDICA THORNS

2018 ◽  
Vol 11 (11) ◽  
pp. 153 ◽  
Author(s):  
Lakshmibai R ◽  
Amirtham D
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Mimosa Pudica ◽  
Age Related ◽  
Dpph Method

Objective: Mimosa pudica is a traditional medicinal plant. The natural antioxidant compounds from plants mop up the free radicals causing cell damage and maintain the biological systems. The aim of the present study is to evaluate the free radical scavenging potential of the ethanolic and aqueous thorn extracts of M. pudica.Methods: The ethanolic and aqueous thorn extracts of M. pudica were analyzed for the phytochemicals and for free radical scavenging activity by 1, 1 diphenyl 1-2-picric hydrazine (DPPH), 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and nitric oxide free radical scavenging method.Results: Flavonoids, saponins, glycosides, alkaloids, terpenoids, and coumarins were the phytoconstituents revealed in ethanolic and aqueous thorn extracts of M. pudica. At 250 μg/mL concentrations, aqueous thorn extracts of M. pudica exhibited 73.41% radical scavenging activity by DPPH method and 26.10% inhibition by nitric oxide free radical scavenging method. However, the ethanolic extracts of M. pudica thorns exhibited 73.35% inhibition by ABTS free radical scavenging method at 250 μg/mL concentrations.Conclusion: The results obtained suggest that the plant extracts from M. pudica could serve as a potential source of antioxidant in slowing down the process of aging and age-related or oxidative stress-related degenerative diseases. Moreover, the isolation of bioactive principle responsible for the antioxidant activity and formulation of novel therapeutic agents can be further studied.

IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ZIZIPHUS RUGOSA LAM. LEAVES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (07) ◽  
pp. 69-75
Author(s):  
S Parashar ◽  
V. Uplanchiwar ◽  
R. K. Gautam ◽  
S. Goyal ◽  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value

Ziziphus rugosa Lam. belongs to the family Rhamnaceae and is found chiefly in deciduous and semi evergreen forest of Western Ghats. The present research was undertaken to establish in vitro antioxidant and in vivo hepatoprotective activity of ethanolic extract of Z.rugosa Lam. leaves. The powdered leaves of Z. rugosa were extracted with ethanol and preliminary phytochemical screening was performed for the presence of various phytoconstituents. DPPH assay and β-glucuronidase inhibition assay were selected for the free radical scavenging activity. For the assessment of hepatoprotective activity, alcohol and CCl4 induced hepatotoxicity model were used. The phytochemical analysis of ethanolic extract showed the presence of alkaloids, saponins and flavonoids. The extract exhibited concentration dependent radical scavenging activity with an IC50 value of 61.88 μg/ml and β –glucoronidase inhibition activity with an IC50 value of 70.61 μg/ml. It was speculated that the Z. rugosa Lam. ethanolic extract shows dosedependent hepatoprotective activity which is equivalent with the standard drug Silymarin. The inhibition of free radicals or free radical scavenging activity is significant in the protection against CCl4 and alcohol induced hepatopathy. Hence, it is likely that the antioxidant activity of ethanolic extract of Z. rugosa Lam. might contribute to the hepatoprotective action.

scholarly journals ANTICANCER AND FREE RADICAL SCAVENGING POTENTIAL OF THE MARINE ALGICOLOUS ENDOPHYTIC FUNGUS CLADOSPORIUM UREDINICOLA

2019 ◽  
pp. 520-524
Author(s):  
VASAVI THIRUMALANADHUNI ◽  
LAVANYA LATHA YERRAGURAVAGARI ◽  
VANI MATHAKALA ◽  
UMA MAHESWARI DEVI PALEMPALLI
Keyword(s):  
Free Radical ◽  
Endophytic Fungus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Normal Cells ◽  
Ic50 Value ◽  
Marine Brown Alga

Objective: The objective was to study the antioxidant and anticancer potential of the endophytic fungus Cladosporium uredinicola, isolated from the marine brown alga Dictyota dichotoma. Methods: Anticancer effect of the endophytic fungal extract was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay using MDA-MB-231 human mammary adenocarcinoma cells as in vitro cancer models. 3T3-L1 pancreatic adipocytes were used as in vitro models for the evaluation of cytotoxic activity against normal cells using MTT assay. Free radical scavenging activity was assessed by 2, 2-diphenyl-1- picrylhydrazyl (DPPH) assay. Results: The ethyl acetate extract of the endophytic fungus showed potent cytotoxic activity against MDA-MB-231 human breast adenocarcinoma cell lines with an inhibitory concentration (IC50) value of 373 μg/ml and a very mild cytotoxic effect on 3T3-L1 Cells with an IC50 value of 2403 μg/ml. DPPH free radical scavenging assay of the extract showed an IC50 value of 359 μg/ml indicating its potential free radical scavenging activity. Conclusion: The results indicated that the endophytic fungus C. uredinicola, isolated from the marine brown alga D. dichotoma, acts as a potential source for anticancer and antioxidant metabolites. Moreover, these anticancer metabolites were observed to be less toxic to the normal cells, which make them prospective therapeutic agents.

scholarly journals Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods

3 Biotech ◽  
2021 ◽  
Vol 11 (5) ◽  
Author(s):  
Chang-Ho Kang ◽  
Jin-Seong Kim ◽  
Hye Min Park ◽  
Seonyoung Kim ◽  
Nam-Soo Paek
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Free Radical ◽  
Lactic Acid Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Scavenging Activity

AbstractCompounds of the cell walls of heat-killed lactic acid bacteria show immunomodulatory properties which boost immunological systems, and are used ad postbiotics (paraprobiotics). In this study, we used 17 different heat-killed isolates as postbiotics and evaluated their anti-inflammatory potential on the expression of proinflammatory mediators and cellular signaling pathways of murine macrophage, RAW 264.7 cells. Bifidobacterium bifidum MG731 showed the high 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (90.6%), followed by Bifidobacterium lactis MG741 (59.6%). The Bi. lactis MG741 showed the high ABTS free radical scavenging activity (99.5%), followed by Lactobacillus plantarum MG989 (98.9%), Lactobacillus salivarius MG242 (97.1%), and Bi. bifidum MG731 (96.1%). In addition, Bi. bifidum MG731 showed the lowest nitric oxide production (4.28 µM), followed by B. lactis MG741 (10.80 µM), L. salivarius MG242 (14.60 µM), and L. plantarum MG989 (19.60 µM). The selected strains showed a decreased nitric oxide production via downregulation of inducible nitric oxide synthase and cyclooxygenase 2, which were upregulated via LPS-stimulated RAW 264.7 macrophages. Short-chain fatty acids (SCFA) including acetic, propionic, and butyric acid were produced by four strains. The Bi. bifidum MG731 showed total SCFAs production (4998.6 µg/g), Bi. lactis MG741 (2613.9 µg/g), L. salivarius MG242 (1456.1 µg/g), and L. plantarum MG989 (630.2 µg/g). These results indicated that the various selected strains may possess an anti-inflammatory potential and provide a molecular basis for the development of functional probiotics.

scholarly journals NITRIC OXIDE PRODUCTION AND ANTIOXIDANT ACTIVITY OF DRIED FRUIT EXTRACTS OF TERMINALIA CHEBULA

2018 ◽  
Vol 11 (5) ◽  
pp. 370
Author(s):  
Aridass Dhanasezhian ◽  
Seetharaman Srivani ◽  
Marimuthu Ragavan Rameshkumar
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Scavenging Activity ◽  
Oxide Production

 Objective: The dried ripe fruits of Terminalia chebula have traditionally been used to treat various ailments as it has a wide spectrum of pharmacological activities. Hence, in the present study, we aimed to explore the antioxidant activity, nitric oxide production, cytotoxicity, and phytocompounds present in the aqueous and methanol extract of T. chebula. Methods: The dry fruits of T. chebula were extracted using water and methanol, and the extracts were concentrated by lyophilization method. Phytochemical analysis was done by gas chromatography and mass spectrometry and Fourier-transform infrared spectroscopy. The free radical scavenging activity of T. chebula was estimated by 1,1diphenyl 2, picrylhydrazyl method. RAW 264.7 cells were stimulated with aqueous and methanol extracts, and the production of nitric oxide was estimated by spectrophotometric method using Griess reagent. Cytotoxicity assay was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and percentage of cell viability was calculated.Results: Aqueous and methanolic extracts of the dry fruit of T. chebula showed non-toxic to RAW 264.7 cells at the concentration of 2 mg and 1.5 mg, respectively. These concentrations showed high free radical scavenging activity and production of optimum concentration of nitric oxide in RAW 264.7 cells.Conclusion: Fruit extracts of T. chebula possess properties of nitric oxide production and high free radical scavenging activity; these properties could be useful in the development of immunomodulatory drugs as well as protection against various human diseases associated with oxidative stress.

scholarly journals ANALISIS FRAKSI AKTIF EKSTRAK DAUN GEDI HIJAU (Abelmoschus manihot L.) DALAM MENANGKAL RADIKAL BEBAS DPPH

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 187
Author(s):  
Maria T Atuani ◽  
Sri Sudewi ◽  
Defny S Wewengkang
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Mobile Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Fraction I

ABSTRACT Gedi (Abelmoschus manihot L.) is a tropical plant of family malvaceae, traditionally known in North Sulawesi as a vegetable plant. Gren gedi leaves from Bitung are known to have activity as free radical scavenging at extract level but have not been tested at the fraction level. This study aims to determine the free radical scavenging activity of the green gedi leaf fractions in counteraction DPPH (1,1-diphenil-2-picrylhydarzyl) free radical. Extraction was done by maceration method using ethanol solvent. The preliminary test of free radical scavenging was carried out by thin layer chromatography (TLC) using the stationary phase of silica gel F254 with the mobile phase of n-hexane : chloroform (1:2) and sprayed with DPPH 0,08 mM. Then it was fractionated using vacuum liquid chromatography (VLC) with the mobile phase based on its polarity, such as combination of n-hexane and ethyl acetate. Each fraction obtained was tested for free radical scavenging activity showed whitish yellow patches on a purple background, which indicates that extracts have free radical scavenging activity. The spectrophotometer measurement results showed that each leaf of green gedi leaves had weak free radical scavenging activity, but still was a free radical scavenger, ie fraction I had an IC50 value of 169,69 μg/mL; fraction II was 826,52 μg/mL; fraction III was 311,13 μg/mL; and fraction IV was 246,80 μg/mL. Fraction V has an IC50 value of 2294,64 μg/mL, this is indicates that the fraction V has no activity as a free radical scavenging compound, because it has very large IC50 value. While vitamin C as a comparison has very strong free radical scavenging activity with IC50 of 3,22 μg/mL. Keywords: Fraction, Extract, Abelmoschus manihot L., DPPH Method. ABSTRAK Gedi (Abelmoschus manihot L.) merupakan tumbuhan tropis family malvaceae, secara tradisional telah lama dikenal di Sulawesi Utara sebagai tanaman sayuran. Daun gedi hijau yang berasal dari Bitung diketahui memiliki aktivitas sebagai penangkap radikal bebas pada tingkat ekstrak namun belum diuji pada tingkat fraksi. Penelitian ini bertujuan untuk mengetahui aktivitas penangkap radikal bebas dari fraksi-fraksi daun gedi hijau dalam menangkal radikal bebas DPPH (1,1-diphenil-2-picrylhydarzyl). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol. Uji pendahuluan aktivitas penangkap radikal bebas dilakukan secara kromatografi lapis tipis (KLT) menggunakan fase diam silika gel F254 dengan fase gerak n-heksana : kloroform (1:2) dan disemprot DPPH 0,08mM. Kemudian difraksinasi menggunakan kromatografi cair vakum (KCV) dengan fase gerak berdasarkan kepolarannya, yakni kombinasi n-heksana dan etil asetat. Hasil uji pendahuluan aktivitas penangkap radikal bebas secara KLT menunjukkan adanya bercak kuning keputih-putihan dengan latar belakang ungu, yang menandakan ekstrak memiliki aktivitas penangkap radikal bebas. Hasil pengukuran secara spektrofotometer menunjukkan bahwa setiap fraksi daun gedi hijau memiliki aktivitas penangkap radikal bebas yang lemah, namun tetap bersifat sebagai penangkap radikal bebas, yakni fraksi I memiliki nilai IC50 sebesar 169,69 μg/mL; fraksi II 826,52 μg/mL; fraksi III 311,13 μg/mL; dan fraksi IV 246,80 μg/mL. Fraksi V memiliki nilai IC50 sebesar 2294,64 μg/mL, menandakan fraksi V tidak memiliki aktivitas sebagai senyawa penangkap radikal bebas, karena memiliki nilai IC50 yang sangat besar. Sedangkan vitamin C sebagai pembanding memiliki aktivitas penangkap radikal bebas sangat kuat dengan IC50 sebesar 3,22 μg/mL.Kata Kunci : Fraksi, Ekstrak, Abelmoschus manihot L., Metode DPPH.

scholarly journals Evaluation of Free Radical Scavenging Potential of Different Bioactive Fractions Present in Boerhavia diffusa Linn. Root Extract: An in-vitro Approach

2020 ◽  
pp. 99-107
Author(s):  
Mohammad Khalid ◽  
Mohammed H. Alqarni ◽  
Ahmed I. Foudah ◽  
Juber Akhtar ◽  
Badruddeen . ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Chloroform Fraction ◽  
Scavenging Activity ◽  
Boerhavia Diffusa

Aim and Objectives: Boerhavia diffusa (B. diffusa) frequently known as punarnava is specifically used to replenish the body. The present work was designed to evaluate the scavenging potential of its bioactive constituents. Materials and Methods: The different fractions of B. diffusa root methanolic extract were examined for phenolic, flavonoids contents, DPPH free radical and Nitric oxide scavenging activities. Further antioxidant activity was evaluated by ABTS free radical scavenging method and also from the reducing potential scavenging activity. The total phenolic content in different fractions by using various solvent like ethanol, diethyl ether, chloroform, ethyl acetate, and n-butanol were carried out to find the antioxidant activities. Results: The phenolic content was highest in ethanolic fraction that was significantly comparable with ascorbic acid. The flavonoid content was highest in ethanol fraction (41.93 ± 3.92 µg/mL) followed by n-butanol fraction (31.68 ± 1.72 µg/mL), then ethyl acetate fraction (29.67 ± 2.83 µg/mL) and least in chloroform fraction (16.91 ± 2.74 µg/mL). The ethanolic fraction of B. diffusa also showed highest DPPH radical scavenging activity (101.29 ± 3.78) when compared with other fractions of same extract using different solvent phases. Moreover the nitric oxide scavenging activity of ethanolic fraction was maximum (82.31 ± 2.83) than different fractions. The ethanolic fraction also showed improved ABTS radical scavenging activity (81.73 ± 2.73 mg/mL) while chloroform fraction showed poor ABTS radical cation scavenging activity (29.51 ± 2.79 mg/mL). Conclusions: The study concludes that Boerhavia diffusa has rich and ample source of phenolic acid and flavonoids. Among all fractions the ethanolic has potent antioxidant activity, which shows its significance for a better novel approach.

scholarly journals Antioxidant Screening on the Whole Plant of Cyanthillium cinereum (L) H.Rob

2021 ◽  
Vol 8 (7) ◽  
pp. 458-461
Author(s):  
Mohammed Shihab K. K. ◽  
Rajagopal P. L. ◽  
Nasila K.
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Whole Plant

Methanolic extract of the whole plant of Cyanthillium cinereum (L) H.Robwasevaluated for free radical scavenging activity by using Nitric oxide scavenging activity (NO) and Reduction of 1, 1-Diphenyl -2-Picryl Hydrazyl (DPPH) free radical scavenging activity. Throughout the studies the plant extract showed potent antioxidant activity and the activity was found to be concentration dependent which may be attributed to the high flavonoid content of the plant. Keywords: Cyanthillium cinereum (L) H.Rob, DPPH, NO.

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