scholarly journals CONCENTRATION DEPENDENT EFFECTS OF CARBOXYMETHYL CHITOSAN ON DENTIN REMINERALIZATION WITH AMORPHOUS CALCIUM PHOSPHATE

2020 ◽  
pp. 31-33
Author(s):  
HASTI DWI SETIATI ◽  
ENDANG SUPRASTIWI ◽  
DEWA AYU NYOMAN PUTRI ARTININGSIH ◽  
LUH PUTU TRISNA BUDI UTAMI
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Matrix Protein ◽  
Carboxymethyl Chitosan ◽  
Control Group ◽  
Dentin Matrix Protein ◽  
Group 4 ◽  
Occlusal Surfaces ◽  
Group 5 ◽  
Group 2

Objective: Carboxymethyl chitosan (CMC) is a non-collagenous protein analog which has a similar role as dentin matrix protein 1. CMC stabilizes amorphous calcium phosphate (ACP); hence forming nanocomplexes of CMC-ACP. The purpose of this study was to evaluate the effect of CMC concentration in CMC-ACP on dentin remineralization.Methods: Cavities were formed on the occlusal surfaces of freshly extracted premolar teeth. All samples were demineralized and immersed in phosphate-buffered saline and stored in a shaking incubator at 37°C. The teeth were randomly divided into five groups. Group 1 was control group (no treatment), whereas Groups 2, 3, 4, and 5 were treated with CMC-ACP containing 1%, 2.5%, 5%, and 10% CMC. The remineralized layer on the dentin surface was evaluated using scanning electron microscopy and energy-dispersive X-ray analysis.Results: The highest dentin remineralization capacity was achieved in Group 5 (10% CMC), whereas diminishing effects were observed in Group 4 (5% CMC), Group 3 (2.5% CMC), and Group 2 (1% CMC). Although no significant differences in calcium levels were observed between 2.5%, 5%, and 10% CMC groups, phosphate levels differed significantly in all treatment groups.Conclusion: Optimal dentin remineralization was achieved by the application of CMC–ACP containing 2.5% CMC.

Comparative Evaluation of Casein Phosphopeptide - Amorphous Calcium Phosphate and Fluoride in Managing Early Caries Lesions

2019 ◽  
Vol 70 (10) ◽  
pp. 3746-3749
Author(s):  
Livia Bobu ◽  
Alice Murariu ◽  
Gabi Topor ◽  
Adrian Beznea ◽  
Roxana Vasluianu
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Control Group ◽  
Invasive Treatment ◽  
Treatment Groups ◽  
Non Invasive ◽  
Group 4 ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Caries Lesions

The aim of the present study was to assess the remineralizing potential of 10% casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) -Recaldent used alone or with fluoride (CPP-ACFP) as compared to fluoride mouthrinse for the non-invasive treatment of occlusal non-cavitary caries lesions in vivo.A total of eighty subjects were randomly assigned to four groups, i.e. group 1 - 10% CPP-ACP, group 2- 10% CPP-ACP + 0.2% NaF, group 3- 0.05% NaF mouthrinse and group 4 �control. The lesions were assessed using visual examination and DIAGNOdent measurements. After 12 weeks of using the corresponding product, the results showed that the highest decrease in fluorescence was produced by CPP-ACFP (from 16.28�1.40 to 6.53�0.59, p=0.002), followed by CPP-ACP (from 15.70�1.82 to 8.18�0.70, p=0.004) and fluoride mouthrinse (from 14.32�1.72 to 7.88�0.68, p=0.005), significantly higher than in the control group (p[0.05). No significant differences were found between the three treatment groups.

scholarly journals THE EFFECT OF CARBOXYMETHYL CHITOSAN/AMORPHOUS CALCIUM PHOSPHATE TO GUIDE TISSUE REMINERALIZATION OF DENTIN COLLAGEN

2019 ◽  
pp. 181-183
Author(s):  
ROSDIANA NURUL ANNISA ◽  
NILAKESUMA DJAUHARIE ◽  
ENDANG SUPRASTIWI ◽  
NORMA AVANTI
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Matrix Protein ◽  
Carboxymethyl Chitosan ◽  
Control Group ◽  
Group 14 ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Dentin Collagen

Objective: Carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) can replace the role of dentine matrix protein 1. Guided tissueremineralization (GTR) is a method of extrafibrillar and intrafibrillar collagen remineralization. This study analyzed the ability of CMC/ACP to achieveintrafibrillar and extrafibrillar remineralization on demineralized dentin.Methods: We divided 12 demineralized occlusal cavities into four groups: 7 days control group, 14 days control group, 7 days CMC/ACP group, and14 days CMC/ACP group. In the control group, the cavities were directly filled with temporary restoration material, while the cavities of the CMC/ACPgroups first underwent application of CMC/ACP and were then filled with temporary restoration material. All samples were stored in a 37°C shakingincubator. 12 samples were analyzed by energy-dispersive X-ray (EDX) spectroscopy and four were analyzed by transmission electron microscope(TEM).Results: From day 7 to day 14, there was a significant increase in calcium and phosphate levels within the CMC/APC groups. The phosphate levelswere much lower than the calcium or minerals formed on the 7th day, in the form of hydroxyapatite. TEM analysis shows that the CMC/ACP groupexhibited more intrafibrillar and extrafibrillar remineralization.Conclusion: CMC/ACP can improve the GTR process.

Comparative Evaluation of Casein Phosphopeptide - Amorphous Calcium Phosphate and Fluoride in Managing Early Caries Lesions

2019 ◽  
Vol 70 (10) ◽  
pp. 3746-3749
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Control Group ◽  
Invasive Treatment ◽  
Treatment Groups ◽  
Non Invasive ◽  
Group 4 ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Caries Lesions

The aim of the present study was to assess the remineralizing potential of 10% casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) -Recaldent used alone or with fluoride (CPP-ACFP) as compared to fluoride mouthrinse for the non-invasive treatment of occlusal non-cavitary caries lesions in vivo.A total of eighty subjects were randomly assigned to four groups, i.e. group 1 - 10% CPP-ACP, group 2- 10% CPP-ACP + 0.2% NaF, group 3- 0.05% NaF mouthrinse and group 4 -control. The lesions were assessed using visual examination and DIAGNOdent measurements. After 12 weeks of using the corresponding product, the results showed that the highest decrease in fluorescence was produced by CPP-ACFP (from 16.28±1.40 to 6.53±0.59, p=0.002), followed by CPP-ACP (from 15.70±1.82 to 8.18±0.70, p=0.004) and fluoride mouthrinse (from 14.32±1.72 to 7.88±0.68, p=0.005), significantly higher than in the control group (p<0.05). No significant differences were found between the three treatment groups. Keywords: CPP-ACP, CPP-ACFP, fluoride mouthrinse, remineralization

scholarly journals Effectiveness of medical treatment in overcoming the ureteral double-J stent related symptoms

2012 ◽  
Vol 6 (6) ◽  
pp. 234 ◽  
Author(s):  
Ugur Kuyumcuoglu ◽  
Bilal Eryildirim ◽  
Murat Tuncer ◽  
Gokhan Faydaci ◽  
Fatih Tarhan ◽  
...  
Keyword(s):  
The Body ◽  
Control Group ◽  
Group 4 ◽  
Double J Stent ◽  
Urinary Tract Symptoms ◽  
Stone Treatment ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Background: We investigated whether the frequency of lower urinary tract symptoms (LUTS) increased in patients in whom double-J stents were applied. We also evaluated several medical therapy protocols to treat symptoms related with ureteral stents.Materials and Methods: A total of 108 patients, in whom unilateral double-j stent was applied during ureteral stone treatment, were included. Before the double-J stent was applied, all patients completed storage components of the “International Prostate Symptom Score” (IPSSs), quality of life components of the IPSS (IPSS-QOL) and “Overactive Bladder Questionnaire” (OABq) forms and scores were calculated. After the procedure, cases were randomized into 5 groups, an antiinflammatory was given to Group 1, spasmolytic to Group 2, anticholinergic to Group 3 and α-blocker to Group 4. No additional drug was given to Group 5 as this control group. During the fourth week of the procedure, IPSSs, IPSS-QOL and OABq forms were again completed and scores were compared with the previous ones.Results: When all the cases were evaluated, the IPSSs, IPSS-QOLand OABq scores of patients in whom the double-J stent was applied were statistically significantly higher the procedure. Compared to the control group, the cases where the double-J stent was applied showed a higher IPSSs, IPSS-QOL and OABq scores and none of the medical therapies could prevent this increase.Interpretation: The frequency of LUTS increased in cases where the ureteral stent was applied and discomfort continued as long as the stent stayed in the body.

scholarly journals Zingerone Ameliorates Hepatotoxicity

2020 ◽  
Vol 11 (1) ◽  
pp. 280-284 ◽  
Author(s):  
Julietpoornamathy J ◽  
Parameswari C.S.
Keyword(s):  
The Body ◽  
Control Group ◽  
Medical Sciences ◽  
Toxic Compounds ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Insight Into ◽  
Group 1

In medical sciences, toxicity is an area wherein extensive studies have been carried to improve the diseases as well as to prevent. So, there is a high requirement for novel and improved alternative therapeutic strategies to manage diseases. The liver is the largest gland in the body, which executes several important mechanisms; it stores minerals and vitamins and releases them in periods of need. The main aim of this study was to give a closer insight into potent non- toxic compounds that is capable of modifying the responses. Animals were divided into five equal groups viz control (Group 1), administered with food and water ad libitum, (Group 2) administered with olive oil, (Group 3) administered with zingerone, (Group 4) administered with concanavalin A, (Group 5) administered with cyclosporine A followed by zingerone. Our results revealed significant changes in liver marker enzymes and liver histology of zingerone treated rats when compared to control rats.  A corollary, zingerone has no toxic effect on hepatocytes and was found to be safe at a dose of 10mg/kg b wt and also ameliorates hepatotoxicity.

Bond Integrity of Titanium Surface to Resin Cement After Conditioning with Different Photobiomodulataion Therapy (PBT)

2021 ◽  
Vol 11 (5) ◽  
pp. 970-975
Author(s):  
Khold Al Ahdal ◽  
Laila Al Deeb ◽  
Mohammed S. Bin-Shuwaish ◽  
Rana S. Al-Hamdan ◽  
Modhi Al Deeb ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Testing Machine ◽  
Resin Cement ◽  
Control Group ◽  
Sem Images ◽  
Group 4 ◽  
Modes Of Failure ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

The aim of the present study was to evaluate the efficacy of interfacial bonding between resin cement and titanium alloy conditioned with different Photobiomodulataion Therapy (PBT) in comparison with conventional regimes. Seventy-five samples of titanium bar were segmented polished; and based on conditioning regime divided into five groups (15 each specimens). Group 1: No treatment (control), Group 2: Sand blasting with 120 µm Al2O3, Group 3: Er,Cr:YSGG (ECL), Group 4: Er:YAG laser (EYL), Group 5: Nd:YAG laser (NYL). After different conditioning regimes, resin cement was mixed and applied. All specimens were thermocycled for 500 cycles and positioned on universal testing machine for shear bond strength testing. Modes of failure was assessed by a single examiner using stereomicroscope at 40× magnification. Three pairs of specimens in each group were sputter coated and scanning electron microscope (SEM) images were taken at 1000× magnification using 20 Kv. The highest SBS values were observed in group 2 (14.25±2.29 MPa) and the lowest bond values were displayed in group 1 (6.98 ±0.59 MPa). Titanium alloy abutments conditioned with different laser prototypes ECL (11.22±1.40 MPa), EYL (9.29±1.22 MPa) and NYL (9.12 ±1.84 MPa) exhibited comparable SBS (p > 0.05). Adhesive failures were primarily dominant among all experimental groups. Photo-biomodulation in the form of ECL lasers has the potential to be used as a supplement to sandblasting procedure in conditioning of cement retained titanium alloy implant abutments.

scholarly journals FE-SEM Evaluation of Dental Specimens Prepared by Different Methods forIn VitroContamination

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Vitor Cesar Nakamura ◽  
Simony Hidee Hamoy Kataoka ◽  
Giulio Gavini ◽  
Patrícia Helena Ferrari ◽  
Silvana Cai
Keyword(s):  
Control Group ◽  
Buffer Solution ◽  
Morphological Alterations ◽  
Ultrasonic Bath ◽  
Group 4 ◽  
Cement Removal ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Objective. To evaluate through FE-SEM the cleanliness and dentinal alterations promoted by different methods of dental sample preparation.Methods. Twenty-five human single-rooted teeth were used. The teeth were cleaned and autoclaved in wet medium and randomly divided into 5 groups (), according to the preparation methods employed—control group: no solutions applied; group 1: cement removal and irrigation with 5.25% % EDTA for 4 minutes each; group 2: 17%  % NaOCl (4 minutes ultrasonic bath); group 3: cement removal and 17%     buffer solution + distilled water (10 minutes ultrasonic); group 4: 17%   NaOCl (3 minutes ultrasonic bath). Specimens were analyzed by field emission scanning electron microscope (FE-SEM), at 1500x magnification. Data were submitted to qualitative analysis according to a scoring system and submitted to Kruskal-Wallis test.Results. In ascending order, as to bind parameters, (i) cleanliness: control, group 2, group 3, group 5, and group 4, (ii) dentinal alterations: group 1, group 5, group 2, group 3, and group 4.Conclusion. The proposed protocol was suitable for subsequent microbiological contamination, because it showed less dentinal morphological alterations with increased removal of organic waste.

scholarly journals The Evaluation of Various Restoration Techniques on Internal Adaptation of Composites in Class V Cavities

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
D. Dionysopoulos ◽  
C. Papadopoulos ◽  
E. Koliniotou-Koumpia
Keyword(s):  
Room Temperature ◽  
Control Group ◽  
Class V ◽  
Group 4 ◽  
Restoration Techniques ◽  
Group 3 ◽  
Internal Adaptation ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Aim.The aim of this study was to evaluate the effect of different restoration techniques on the formation of internal microgaps between materials and dentin in class V restorations.Materials and Methods.Twenty-five extracted human premolars were prepared with standardized class V cavity outlines (3 mm × 2 mm × 2 mm). The cavities were randomly divided into 5 groups of 10 cavities each and restored according to manufacturer’s instructions: Group 1: preheating (55°C) conventional composite (Filtek Z250), Group 2: flowable composite (Filtek Flow), Group 3: Filtek Flow + Filtek Z250 light-cured separately, Group 4: Filtek Flow + Filtek Z250 light-cured simultaneously, and Group 5 (control): Filtek Z250 at room temperature (23°C). The specimens were then thermocycled and cross-sectioned through the center of the restoration. Subsequently, impressions were taken, and epoxy resin replicas were made. The internal adaptation of the materials to the axial wall was analyzed under SEM.Results.The preheated Filtek Z250 (Group 1) showed better internal adaptation than the room temperature groups(P<0.05). The combination of Filtek Flow with Filtek Z250 which was light-cured separately (Group 3) exhibited better internal adaptation than control group(P<0.05).Conclusion.Different restoration techniques exhibit different behavior regarding internal adaptation to dentin after photopolymerization.

scholarly journals NADPH Oxidase 4 Signaling in a Ventilator-induced Lung Injury Mouse Model

2021 ◽  
Author(s):  
Sang Hoon Lee ◽  
Mi Hwa Shin ◽  
Ah Young Leem ◽  
Su Hwan Lee ◽  
Kyung Soo Chung ◽  
...  
Keyword(s):  
Lung Injury ◽  
Lavage Fluid ◽  
Lung Damage ◽  
Control Group ◽  
Group 4 ◽  
Ventilator Induced Lung Injury ◽  
Cell Counts ◽  
Group 5 ◽  
Group 2

Abstract For patients with acute respiratory distress syndrome, a ventilator is essential to supply oxygen to tissues, but it may also cause lung damage. We investigated the role of NOX4 in a ventilator-induced lung injury (VILI) model.Wild-type (WT) male C57BL/6J mice and NOX4 knockout (KO) male mice were divided into five groups: (1) control group; (2) high tidal ventilation (HTV) group: WT mice + HTV; (3) NOX4 KO group; (4) NOX4 KO with HTV group; (5) NOX4 inhibitor group: WT mice + HTV + NOX4 inhibitor. In addition, the relationship between EphA2 (which is related to lung injury) and NOX4 was investigated using EphA2 KO mice, and NOX4 levels in the bronchoalveolar lavage fluid (BALF) of 38 patients with pneumonia were examined.In the NOX4 inhibitor group, cell counts and protein concentrations from BALF were significantly lower than those in the HTV group (both, p<0.001). In the NOX4 KO group and the NOX4 inhibitor group, EphA2 levels were significantly lower than those in the HTV group (p<0.001). NOX4 levels were significantly higher in patients with pneumonia and patients who received ventilator treatment in the ICU.In the VILI model, it may be possible to block VILI using NOX4 antibodies.

scholarly journals Treatment of ovarian cysts in sows – a field trial

2008 ◽  
Vol 52 (No. 9) ◽  
pp. 413-418 ◽  
Author(s):  
S. Cech ◽  
R. Dolezel
Keyword(s):  
The Other ◽  
Ovarian Cysts ◽  
Control Group ◽  
Initial Examination ◽  
Interval Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Different procedures of treatment of large follicular ovarian cysts in 177 sows using GnRH, hCG and PGF2α are evaluated in this study. Ovarian cysts were diagnosed by transcutaneous ultrasonography, which was a part of routine pregnancy diagnosis. No treatment was performed in the control group (Group 1, <i>n</i> = 29); the method of treatment used in the other groups immediately after the diagnosis was intramuscular administration of lecirelin in doses 50 µg (Group 2, <i>n</i> = 28), 100 µg (Group 3, <i>n</i> = 27) and 200 µg divided into 2 equal doses administered at a 12-hour interval (Group 4, <i>n<i> = 25) and of hCG in doses 1 500 IU (Group 5, <i>n</i> = 23), 3 000 IU (Group 6, <i>n</i> = 21), and 250 µg of cloprostenol (Group 7, <i>n</i> = 24). Insemination rate (IR), conception rate (CR) in inseminated sows, pregnancy rate (PR = recovery rate), treatment-insemination interval (TII) and treatment-pregnancy interval (TPI) within 42 days after the initial examination were evaluated. In addition PR in groups of sows divided according to parity (1–3, 4–6 and ≥ 7) were also evaluated. IR and PR were higher in Group 4 (84.0% and 44.0%) and lower in Group 1 (17.2% and 6.9%) in comparison with the other groups (<i>P</i> < 0.001 and <i>P</i> < 0.05). CR, TII and TPI did not differ between the experimental groups. PR were similar in sows with different parity. The study proved a positive response in sows with large follicular ovarian cysts to the treatment consisting of 2 administrations of 100 µg GnRH at a 12-hour interval.

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