scholarly journals ALPHA AMYLASE AND ALPHA GLUCOSIDASE INHIBITION ACTIVITY OF SELECTED EDIBLE SEAWEEDS FROM SOUTH COAST AREA OF INDIA

2017 ◽  
Vol 9 (6) ◽  
pp. 64 ◽  
Author(s):  
P. Reka ◽  
Thahira Banu A. ◽  
M. Seethalakshmi
Keyword(s):  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Alpha Amylase ◽  
Postprandial Blood Glucose ◽  
Inhibition Activity ◽  
Gracilaria Edulis ◽  
Edible Seaweeds ◽  
Daily Diet ◽  
Postprandial Blood Glucose Level ◽  
Alpha Glucosidase

Objective: The present work was to investigate the alpha amylase and alpha-glucosidase inhibitory activity of the selected edible seaweeds.Methods: The seaweeds namely Acanthophora spicifera, Gracilaria corticata, Gracilaria edulis, Ulva lactuca and Ulva reticulata were selected for this study. Six and eight hours of ethanol and aqueous extract were used for the estimation of alpha amylase using DNS method and alpha-glucosidase inhibition activity.Results: The study reported that the solvent from ethanol and aqueous in eight hours of extraction showed a higher inhibitory activity than six hours of extraction. Maximum of 89.1±0.96 and 79.55±3.08 percent of alpha-amylase and alpha-glucosidase inhibition activity were detected in the eight hours of aqueous extract (0.5 ml) of Ulva reticulata and Gracilaria edulis respectively. All the selected edible seaweeds had significant differences (p<0.05) in alpha amylase and alpha glucosidase inhibition activity between the selected seaweeds with different extracts.Conclusion: It was concluded that all the selected edible seaweeds have the potential to act as a potent inhibitor of the carbohydrate hydrolyzing enzyme. Thus, it was clear from the study that seaweeds incorporated in small amounts in the dishes consumed in the daily diet can bring a control on postprandial blood glucose level.

scholarly journals IN-VITRO ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE INHIBITION ACTIVITY OF DECALEPIS HAMILTONII WIGHT AND ARN

2021 ◽  
pp. 1-3
Author(s):  
Srinivasan S ◽  
Rayar A
Keyword(s):  
Methanolic Extract ◽  
Biologically Active ◽  
Alpha Amylase ◽  
Postprandial Blood Glucose ◽  
Herbal Extracts ◽  
Inhibition Activity ◽  
H Nmr ◽  
Decalepis Hamiltonii ◽  
Alpha Glucosidase

Biologically active polyphenol, D-catechin was isolated from Decalepis hamiltonii and characterized by IR, 1 H- NMR 13C- NMR and evaluation of its in-vitro α-amylase and α-glucosidase inhibition activities. Bioactive compounds are deposited in many parts of the plants, such as in roots, stems, leaves, flowers, fruits and seeds. They protect the plants from diseases and contribute aroma, color and flavor. Inhibitors 𝛼 - amylase and 𝛼-glucosidase delay the breaking down of carbohydrates in the small intestine and lower the postprandial blood glucose excursion. Methanolic extract showed the greater % inhibition of the alpha glucosidase enzyme compared to D-catechin. The herbal extracts produced a slightly weak alpha glucosidase enzyme inhibition when compared with alpha amylase.

Exploration of Diosmin to control diabitis and its complications-an in vitro and in silico approach

2020 ◽  
Vol 16 ◽  
Author(s):  
Kushagra Dubey ◽  
Raghvendra Dubey ◽  
Revathi Gupta ◽  
Arun Gupta
Keyword(s):  
Molecular Docking ◽  
Aldose Reductase ◽  
Inhibitory Activity ◽  
Blood Lipid ◽  
Docking Studies ◽  
Alpha Amylase ◽  
Lipid Lowering ◽  
Molecular Docking Studies ◽  
Alpha Glucosidase

Background: Diosmin is a flavonoid obtained from the citrus fruits of the plants. Diosmin has blood lipid lowering activities, antioxidant activity, enhances venous tone and microcirculation, protects capillaries, mainly by reducing systemic oxidative stress. Objective: The present study demonstrates the potential of Diosmin against the enzymes aldose reductase, α-glucosidase, and α-amylase involved in diabetes and its complications by in vitro evaluation and reverse molecular docking studies. Method: The assay of aldose reductase was performed by using NADPH as starting material and DL-Glyceraldehyde as a substrate. DNS method was used for alpha amylase inhibition and in alpha glucosidase inhibitory activity p-nitrophenyl glucopyranoside (pNPG) was used as substrate. The reverse molecular docking studies was performed by using Molegro software (MVD) with grid resolution of 30 Å. Result: Diosmin shows potent inhibitory effect against aldose reductase (IC50:333.88±0.04 µg/mL), α-glucosidase (IC50:410.3±0.01 µg/mL) and α-amylase (IC50: 404.22±0.02 µg/mL) respectively. The standard drugs shows moderate inhibitory activity for enzymes. The MolDock Score of Diosmin was -224.127 against aldose reductase, -168.17 against α-glucosidase and -176.013 against α-amylase respectively, which was much higher than standard drugs. Conclusion: From the result it was concluded that diosmin was a potentially inhibitor of aldose reductase, alpha amylase and alpha glucosidase enzymes then the standard drugs and it will be helpful in the management of diabetes and its complications. This will also be benevolent to decrease the socio economical burden on the middle class family of the society.

Antioxidant and Anti-diabetic Properties of Aqueous Methanol Extraction of Nephelium mutabile (Pulasan) Leaves

2020 ◽  
Vol 2 (1) ◽  
pp. 27
Keyword(s):  
Column Chromatography ◽  
Inhibitory Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Alpha Amylase ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Aqueous Methanol ◽  
Ic50 Value ◽  
Alpha Glucosidase

Nephelium mutabile is a tropical plant of Sapindaceae family which originated from the Malay Peninsula and used in traditional medicine. This study focused on investigating the antioxidant and anti-diabetic properties of Nephelium mutabile leaves. The aqueous methanol (80%) crude extract of Nephelium mutabile leaves were fractionated via gravity column chromatography and thin-layer chromatography. A total of 17 fractions were obtained from column chromatography and subsequently pooled together to yield eight fractions. The pooled fractions were examined for their antioxidant properties via DPPH assay, Folin-Ciocalteu assay, and aluminum chloride colorimetric assay and tested for anti-diabetic properties using alpha-amylase and alpha-glucosidase inhibitory assay. DPPH assay showed that fraction 8 has the highest free radical scavenging activity (EC50 =88.0 μg/mL) followed by fraction 7 (EC50 =225.0 μg/mL). Fraction 8 showed the highest total phenolic and flavonoid content (221.1 μg GAE/mg, 222.4 μg QE/mg) respectively followed by fraction 7 (166.5 μg GAE/mg, 213.9 μg QE/mg) respectively. The most potent alpha-amylase inhibitory activity is observed in fraction 8 with an IC50 value of (41.0 μg/mL) followed by fraction 7 (90.6 μg/mL). In the alpha-glucosidase inhibitory activity, fraction 8 with IC50 value 160.0 μg/mL followed by fraction 7 with IC50 value 50.6 μg/mL showed the best inhibition activity. In conclusion, Nephelium mutabile leaves showed potential antioxidant, and anti-diabetic properties and fractions 8 and 7 should be the focus of future studies.

Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose

2003 ◽  
Vol 85 (2-3) ◽  
pp. 283-287 ◽  
Author(s):  
Young-Jun Shim ◽  
Ho-Kyung Doo ◽  
Se-Young Ahn ◽  
Yong-Suk Kim ◽  
Je-Kyung Seong ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Inhibitory Effect ◽  
Postprandial Blood Glucose ◽  
Glucosidase Activity ◽  
Rhus Chinensis ◽  
Alpha Glucosidase

scholarly journals MOLECULAR DOCKING STUDIES OF ISOLATED COMPOUNDS ANDROGRAPHOLIDE AND BETULIN FROM METHANOLIC LEAVES EXTRACT OF ANDROGRAPHIS ECHIOIDES AS ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE ACTIVATORS

2021 ◽  
pp. 121-129
Author(s):  
S. GURUPRIYA ◽  
L. CATHRINE
Keyword(s):  
Inhibitory Activity ◽  
In Silico ◽  
Structural Parameters ◽  
Docking Studies ◽  
Alpha Amylase ◽  
In Silico Docking ◽  
Lamarckian Genetic Algorithm ◽  
First Time ◽  
Recent Version ◽  
Alpha Glucosidase

Objective: The purpose of this study is to isolate and characterize the andrographolide and betulin from methanolic leaves extract of Andrographis echioides and also used to evaluate the alpha-amylase and alpha-glucosidase inhibitory activity of isolated compounds using in silico docking studies. Methods: The isolation was done using column chromatography using gradient mobile phase. Structural elucidation was carried out on the basis of spectral analysis. In this view, andrographolide and betulin were prepared for the docking evaluation. In silico docking studies were carried out using a recent version of Auto Dock 4.2, which has the basic principle of Lamarckian genetic algorithm. Results: On the basis of the spectral data, the compounds have been established as andrographolide and betulin are being reported from this plant for the first time. The result showed that the andrographolide showed a binding affinity for amylase: (-7.9 kcal/mol) and for glucosidase (-7.2 kcal/mol) while betulin showed (-8.6 kcal/mol) and (-5.2 kcal/mol), respectively. Conclusion: Therefore, it is suggested that isolated compounds andrographolide and betulin contributed excellent α-amylase and α-glucosidase inhibitory activity because of its structural parameters. Thus, these isolated compounds can be effectively used as drugs for treating diabetes which is predicted on the basis of docking scores.

In-Vitro and In-Silico Alpha Glucucosidase Inhibitory activity of Oroxylum indicum

2021 ◽  
pp. 119-125
Author(s):  
Gejalakshmi S. ◽  
Harikrishnan N. ◽  
Anas S. Mohameid
Keyword(s):  
Molecular Docking ◽  
Inhibitory Activity ◽  
In Silico ◽  
Docking Study ◽  
Alpha Amylase ◽  
Scavenging Activity ◽  
Molecular Docking Study ◽  
Ic50 Value ◽  
Alpha Glucosidase

Background: Diabetes mellitus is a metabolic condition characterized by elevated blood glucose levels in the bloodstream. It occurs due to the inadequate amount of insulin secreted in the body or resistance of insulin receptors. Objective: In the present study, for its effect on alpha-amylase and alpha-glucosidase enzymes, Oroxylum indicuma flavone glycoside was assessed using in-vitro assays by removing the respective enzymes from whole wheat and barley in conjunction with in-silico analysis. Method: in-vitro alpha amylase inhibitory activity and in-vitro alpha glucosidase inhibitory activity was performed using acarbose as a standard drug. The molecular docking study was performed using Schrodinger (Maestro V 11.5) software. The parameters glide score, Lipinski rule for drug likeliness, bioactive scoring and ADME properties were assessed in the docking study. In addition, baicalein's antioxidant function was assessed using DPPH assay, nitric oxide scavenging activity. The cytotoxicity of Oroxylum indicumwas evaluated using the Brine shrimp lethality assay. Results: The alpha-amylase assay performed showed IC50 value of 48.40 µg/ml for Oroxylum indicumwhereas alpha-glucosidase assay showed an IC50 value of 16.03 µg/ml. Oroxylum indicumshows the glide score of-5.565 with 5EOF and glide score of -5.339 with 5NN8 in the molecular docking study. The highest percentage of DPPH radical scavenging activity and nitrous oxide scavenging activity were found to be.27% at160 µg/ml and 50.02% at the concentrations of 160 µg/ml respectively. Conclusion: Based on further in vivo and clinical trials, Oroxylum indicummay be used for the management of hyperglycaemia.

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