Evaluation of anti-inflammatory activity and anti-oxidant potential of portulaca quadrifida linn.

Portulaca quadrifidaL. (PQ) having the phytochemicals like Alkaloids, flavonoids, Saponins, tannins, glycosides, carbohydrates, aminoacids, triterpenoids. The present study was performed to evaluate the antioxidant and anti-inflammatory activities by using acetic acid induced vascular permeability model in mice & acetic acid induced colitis in rats significantly. PQ (100 mg/kg, p.o.) presented a significant anti-inflammatory activity towards acetic acid induced vascular permeability model in mice in comparison to Diclofenac sodium(10 mg/kg, s.c.) and acetic acid induced colitis in rats in comparison to 5-ASA. Our findings suggest that, PQ contains potential antioxidant and anti-inflammatory compounds which will aid us to conduct bioactivity guided isolation & characterization of leading compounds in due course.

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ANTI-INFLAMMATORY ACTIVITY OF CURCUMIN AND CAPSAICIN AUGMENTED IN COMBINATION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i6.18635 ◽

2017 ◽

Vol 9 (6) ◽

pp. 145

Author(s):

Thriveni Vasanth Kumar ◽

Manjunatha H. ◽

Rajesh Kp

Keyword(s):

Acetic Acid ◽

Protective Effect ◽

Vascular Permeability ◽

Diclofenac Sodium ◽

Significant Inhibition ◽

Inflammatory Activity ◽

Anti Inflammatory ◽

The Individual

Objective: Dietary curcumin and capsaicin are well known for their health beneficial potencies. The current study was done to assess the anti-inflammatory activity of curcumin, capsaicin and their combination by employing in vitro and in vivo models.Methods: We investigated the protective effect of curcumin, capsaicin and their combination using in vitro heat induced human red blood cell (HRBC) membrane stabilisation, in vivo 3% agar induced leukocyte mobilisation and acetic acid induced vascular permeability assay.Results: Curcumin, capsaicin and their combination exhibited concentration dependent protective effect against heat-induced HRBC membrane destabilisation, while combined curcumin and capsaicin restored 87.0±0.64 % membrane stability and it is found to be better than curcumin, capsaicin and diclofenac sodium (75.0±0.25. 72±0.9 and 80.0±0.31 %) protective effect. In agar suspension induced leukocyte mobilization assay, the combined curcumin and capsaicin had shown 39.5±1.58 % of inhibition compared to individual curcumin and capsaicin, which showed moderate inhibition of 16.0±3.14 and 21.6±2.17 % respectively. Besides, the combined curcumin and capsaicin had shown highly significant inhibition of acetic acid-induced vascular permeability in rats (62.0±3.14 %), whereas individual curcumin and capsaicin showed moderate inhibition of vascular permeability with 36.0±2.41 and 43.0±1.92 % respectively.Conclusion: This study demonstrates the significant anti-inflammatory property of combined curcumin and capsaicin at half of the individual concentration of curcumin and capsaicin.

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Evaluation of the Anti-Inflammatory and Analgesic Activities of the Total Saponins Extracted from Fermented Polygala japonica Houtt

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.641-642.931 ◽

2013 ◽

Vol 641-642 ◽

pp. 931-934

Author(s):

Ming Xing Liu ◽

Ting Zhu ◽

She Ne Guo ◽

Hong Da Zhu

Keyword(s):

Experimental Study ◽

Acetic Acid ◽

Vascular Permeability ◽

Analgesic Activity ◽

Inflammatory Activity ◽

Hot Plate ◽

Plate Models ◽

Anti Inflammatory ◽

Analgesic Activities

This study was to investigate the anti-inflammatory and analgesic activities of the total saponins extracted from fermented Polygala japonica Houtt (FPH) compared with that of unfermented Polygala japonica Houtt (UFPH). The total saponins extracted from FPH and UFPH were evaluated for anti-inflammatory activity in xylene-induced ear swelling and acetic acid-induced vascular permeability models in mice, analgesic activity in acetic acid-induced writhing and hot plate models in mice. The total saponins extracted from FPH had the significant anti-inflammatory (p<0.001) and analgesic (p<0.01) activities with the doses of 6 g/kg b.w. in mice. The results of this experimental study thus strongly support the potential significant use of the total saponins extracted from FPH for pain and inflammatory.

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EFEK ANTIINFLAMASI PERASAN KERING BUAH Morinda citrifolia Linn SECARA PER ORAL PADA TIKUS PUTIH

Journal of Biological Researches ◽

10.23869/bphjbr.9.1.200311 ◽

2003 ◽

Vol 9 (1) ◽

pp. 53-55

Author(s):

Wahjo Dyatmiko ◽

Suprapto Maat ◽

Idha Kusumawati ◽

Gemparing Bayu Wiyoto

Keyword(s):

Body Weight ◽

Acetic Acid ◽

Vascular Permeability ◽

Test Method ◽

Morinda Citrifolia ◽

Inflammatory Activity ◽

Writhing Test ◽

Bioactivity Assay ◽

Anti Inflammatory ◽

Per Oral

Research on anti inflammatory activity of the fruit of Morinda citrifolia Linn. Or known as pace in Indonesia language has been done by using three methods of assays i.e. anti inflammatory bioactivity assay by measuring the reduction of carrageen-induced oedema in rats analgesic bioactivity assay using writhing test method on mice, and vascular permeability bioactivity assay on mice induced by acetic acid. Results showed that dried juice of the fruit at the dose 1500 mg/kg body weight decreased the volume of oedema up to 64 percent and reduced the pain up to 54 percent. At the dose 2100 mg/kg body weight the dried juice reduced the vascular permeability up to 72 percent.

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Anti-inflammatory activity of Buchholzia coriacea Engl. (Capparaceae) leaf extract: evaluation of components of the inflammatory response involved

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2014-0046 ◽

2015 ◽

Vol 12 (2) ◽

Cited By ~ 2

Author(s):

Adaobi C. Ezike ◽

Collins A. Onyeto ◽

Ifeoma A. Nwabunike ◽

Florence N. Mbaoji ◽

Blessing E. Attah ◽

...

Keyword(s):

Acetic Acid ◽

Inflammatory Response ◽

Red Blood Cells ◽

Vascular Permeability ◽

Blood Cells ◽

Leaf Extract ◽

Inflammatory Activity ◽

Hypotonic Solution ◽

Leukocyte Counts ◽

Anti Inflammatory

Abstract: Earlier studies in our laboratory demonstrated the anti-inflammatory activity of: The effects of methanol leaf extract of: The extract (200 and 400 mg/kg) inhibited acetic acid-induced increase in vascular permeability in a non-dose-related manner and significantly (p<0.05) reduced the total and differential leukocyte counts, respectively, in a dose-related manner. It also significantly (p<0.05) inhibited complement-induced hemolysis of sheep red blood cells (40–72 %) and moderately inhibited heat- (6 %) and hypotonic solution-(24 %) induced hemolysis: Results demonstrated that the anti-inflammatory activity of

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Analgesic and anti-inflammatory activity of aqueous extract of Bubonium graveolens

Biomedical Research and Therapy ◽

10.15419/bmrat.v7i9.632 ◽

2020 ◽

Vol 7 (9) ◽

pp. 4002-4009

Author(s):

Khadidja Fyad ◽

Nasser Belboukhari ◽

Aminata Ould El Hadj-Khelil ◽

Khaled Sekkoum

Keyword(s):

Acetic Acid ◽

Aqueous Extract ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

Paw Edema ◽

Inflammatory Agent ◽

Anti Inflammatory ◽

Traditional Pharmacopoeia ◽

Effective Use ◽

Pain Inhibition

Introduction: Bubonium graveolens is used in traditional pharmacopoeia against imbalances of the gastrointestinal tract, cephalic pains, and bronchitis, and as an anti-inflammatory agent. Methods: We have investigated the analgesic and anti-inflammatory activities of the aqueous extract of Bubonium graveolens in male mice of strain NMRI Albinos, weighing between 22 g and 38 g. Acetic acid was used to induce writhes in the mice and inflammation of paw edema. Results: Evaluation of the analgesic activity showed that the aqueous extract at 150 mg/kg of the plant induced a decrease in the number of abdominal cramps caused by 1% acetic acid. The aqueous extract of the plant had an analgesic effect almost equal to that of Diclofenac; in fact, the latter caused a pain inhibition of 49 ± 1.1% while Bubonium graveolens caused a pain inhibition of 49.6 ± 2.1%, at the concentration of 150 mg/kg. Evaluation of the percentage of inhibition showed that the aqueous extract of Bubonium graveolens had a better anti-inflammatory activity compared to Diclofenac sodium during the treatment duration (69.57% — 56.52% at 60 min; 71.43% — 50.00% at 120 min, and 75.00% — 66.67% at 180 min). Conclusion: The results of this research indicate that Bubonium graveolens inhibits inflammation and could explain its effective use in traditional medicine.

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Suppressions of Serotonin-Induced Increased Vascular Permeability and Leukocyte Infiltration byBixa orellanaLeaf Extract

BioMed Research International ◽

10.1155/2013/463145 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Yoke Keong Yong ◽

NurShahira Sulaiman ◽

Muhammad Nazrul Hakim ◽

Gwendoline Ee Cheng Lian ◽

Zainul Amirudin Zakaria ◽

...

Keyword(s):

Vascular Permeability ◽

Inflammatory Activity ◽

Blue Dye ◽

Leukocyte Infiltration ◽

Permeability Model ◽

Air Pouch ◽

Rat Paw Edema ◽

Anti Inflammatory ◽

Pharmacological Screening ◽

Air Pouch Model

The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract ofBixa orellana(AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg−1) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats’ paws were observed with AEBO at the dose of 150 mg kg−1. Pharmacological screening of the extract showed significant (P<0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release.

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Fatty Acid Profile and Anti-Inflammatory Activity of Fixed Plant Oils

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.79403 ◽

2017 ◽

Vol 45 (1) ◽

pp. 8 ◽

Cited By ~ 4

Author(s):

Selena Maia de Morais ◽

José Eranildo Teles do Nascimento ◽

Antonio Adailson de Sousa Silva ◽

José Eduardo Ribeiro Honório Junior ◽

Diana Célia Sousa Nunes Pinheiro ◽

...

Keyword(s):

Fatty Acids ◽

Acetic Acid ◽

Fatty Acid ◽

Linoleic Acid ◽

Vascular Permeability ◽

Unsaturated Fatty Acids ◽

Healing Process ◽

Inflammatory Activity ◽

Ear Edema ◽

Anti Inflammatory

Background: Many seed oils have been used as anti-inflammatory agents, administred by ingestion or topical application in traditional medicine. The objective of this research was to perform a chemical analysis of fatty profile and a pharmacological study through a topical experiment of TPA-induced ear edema test and an internal assay - acetic acid-induced vascular permeability in Swiss mice of some fixed oils popularly used for inflammatory problems, trying to confirm their action.Materials, Methods & Results: Fixed lipids of Ouratea fieldingiana (batiputá), Caryocar coreaceum (pequi), Annacardium occidentale (cashew-nuts), Cocos nucifera (coco-da-bahia), Byrsonima crassifolia (murici) e Elaeis guineenses (palm) were selected for the identification of fatty acids profile by gas chromatography coupled to mass spectrometry (GC/MS) analysis and evaluation of anti-inflammatory activity by TPA-induced ear edema test and acetic acid-induced vascular permeability in Swiss male mice. The oils were purchased in local markets or extracted in Soxhlet apparatus with hexane. The oils of cashew nut, murici fruit, and pequi nut presented a high percentage of unsaturated fatty acids (81.80, 74.46 and 60.72 %, respectively). In the oils of batiputá and murici, linoleic acid was the main unsaturated fatty acid (45.06% and 74.66%, respectively) and oleic acid was main constituent in cashew nut, pequi and palm seed oils. Batiputá and palm oils exibit approximately equivalent content of saturated and unsaturated fatty acid and coconut oil more saturated fatty acids (80.72%) with predominance of lauric acid. The result of TPA-induced ear edema test revealed that all oils presented similar anti-inflammatory activity. In the acetic acid-induced vascular permeability model, the oil of O. fieldingiana was the only one who showed anti-inflammatory activity, while C. coreaceum and B. crassifolia oils showed pro-inflammatory activities. The presence of phenols and flavonoids was evaluated in the O. fieldingiana oil by spectrophometric methods.Discussion: All the oils showed anti-inflammatory action in the TPA-induced ear edema, probably the action of unsaturated fatty acids was more important in topical application, nevertheless in internal inflammation process the presence of antioxidant phenolic compounds could contribute to the higher activity of the oil from O. fieldingiana. The effect of linoleic and oleic acids was demonstrated on the inflammatory response of the skin during the healing process and on the release of pro-inflammatory cytokines by rat neutrophils in a prevoius study using sunflower oil. Both oleic and linoleic acids increased the wound healing tissue mass. The total protein and DNA contents of the wounds were increased by the treatment with linoleic acid. This pro-inflammatory effect of oleic and linoleic acids may contribute to the wound healing process. In this study with six plant oils, some of them have higher content in linoleic acid and others oleic acid is the major constituent so the antiinflamatory action on ear edema can be associated to these two unsaturaded fatty acids mechanism of action. In the internal model, probably other chemical constituents revealed in Ouratea fieldingiana as phenols, condensed tannins, flavones and flavanones, could contribute to the anti-inflammatory activity.

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Patchoulene Epoxide Isolated from Patchouli Oil Suppresses Acute Inflammation through Inhibition of NF-κB and Downregulation of COX-2/iNOS

Mediators of Inflammation ◽

10.1155/2017/1089028 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 5

Author(s):

Jia-Li Liang ◽

Jia-Zhen Wu ◽

Yu-Hong Liu ◽

Zhen-Biao Zhang ◽

Qi-Duan Wu ◽

...

Keyword(s):

Nitric Oxide ◽

Acetic Acid ◽

Vascular Permeability ◽

Inflammatory Activity ◽

Compositional Variation ◽

Paw Edema ◽

Unknown Compound ◽

Ear Edema ◽

Cox 2 ◽

Anti Inflammatory

According to the GC-MS analysis, compositional variation was observed between samples of patchouli oil, of which an unknown compound identified as patchoulene epoxide (PAO) was found only in the long-stored oil, whose biological activity still remains unknown. Therefore, the present study aimed to evaluate the potential anti-inflammatory activity with three in vivo inflammatory models: xylene-induced ear edema, acetic acid-induced vascular permeability, and carrageenan-induced paw edema. Further investigation into its underlying mechanism on carrageenan-induced paw edema was conducted. Results demonstrated that PAO significantly inhibited the ear edema induced by xylene, lowered vascular permeability induced by acetic acid and decreased the paw edema induced by carrageenan. Moreover, PAO markedly decreased levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), prostaglandin E2 (PGE2), and nitric oxide (NO), but increased levels of interleukin-4 (IL-4) and interleukin-10 (IL-10). PAO was also shown to significantly downregulate the protein and mRNA expressions of cyclooxygenase-2 (COX-2) and inducible nitric-oxide synthase (iNOS). Western blot analysis revealed that PAO remarkably inhibited p50 and p65 translocation from the cytosol to the nucleus by suppressing IKKβ and IκBα phosphorylation. In conclusion, PAO exhibited potent anti-inflammatory activity probably by suppressing the activation of iNOS, COX-2 and NF-κB signaling pathways.

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