scholarly journals The Effect of Some Verbascum Plant Extracts on Cytoplasmic Membrane of Multidrug Resistant Bacteria by Flow Cytometry

2021 ◽  
Vol 6 (5) ◽  
pp. 035-041
Author(s):  
Yener Tekeli ◽  
Elif Esra Altuner ◽  
Hatice Danahaliloglu ◽  
Tuba Tekeli ◽  
Yelda Guzel
Keyword(s):  
Flow Cytometry ◽  
Plant Extracts ◽  
Cytoplasmic Membrane ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

scholarly journals Antibacterial activity of Lamiaceae plant extracts in clinical isolates of multidrug-resistant bacteria

2018 ◽  
Vol 90 (2) ◽  
pp. 1665-1670 ◽  
Author(s):  
FELIPE V. DE ASSIS ◽  
FLÁVIA L. SIQUEIRA ◽  
ISABELA E. GONÇALVES ◽  
RAFAEL P. LACERDA ◽  
RAFAELA A. NASCIMENTO ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

scholarly journals Potent Antimicrobial and Antibiofilm Activities of Feleucin-K3 Analogs Modified by α-(4-Pentenyl)-Ala against Multidrug-Resistant Bacteria

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 761
Author(s):  
Xiaomin Guo ◽  
Tiantian Yan ◽  
Jing Rao ◽  
Xin Yue ◽  
Xiong Pei ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Cytoplasmic Membrane ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Unnatural Amino Acid ◽  
Cellular Component ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

The dramatic increase in antimicrobial resistance (AMR) highlights an urgent need to develop new antimicrobial therapies. Thus, antimicrobial peptides (AMPs) have emerged as promising novel antibiotic alternatives. Feleucin-K3 is an amphiphilic α-helical nonapeptide that has powerful antimicrobial activity. In our previous study, it was found that the fourth residue of Feleucin-K3 is important for antimicrobial activity. After α-(4-pentenyl)-Ala was introduced into this position, both the antimicrobial activity and stability were greatly improved. Herein, to improve the limitations of Feleucin-K3, this unnatural amino acid was further introduced into different positions of Feleucin-K3. Among these synthetic Feleucin-K3 analogs, the N-terminal-substituted analog Feleucin-K65 (K65) and C-terminal-substituted analog Feleucin-K70 (K70) had preferable antimicrobial activity. In particular, their antimicrobial activities against multidrug-resistant bacteria were more potent than that of antibiotics. The stabilities of these peptides in salt and serum environments were improved compared with those of Feleucin-K3. In addition, these analogs had low hemolytic activity and AMR. More importantly, they effectively inhibited biofilm formation and exhibited considerable efficacy compared with traditional antibiotics against biofilm infection caused by methicillin-resistant Staphylococcus aureus (MRSA). In antimicrobial mechanism studies, K65 and K70 mainly permeated the outer membrane and depolarized the cytoplasmic membrane, resulting in cellular component leakage and cell death. In summary, analogs K65 and K70 are potential antimicrobial alternatives to solve the antibiotic crisis.

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