scholarly journals Distributions of intravenous injected iodine nanoparticles in orthotopic u87 human glioma xenografts over time and tumor therapy

Nanomedicine ◽  
2020 ◽  
Vol 15 (24) ◽  
pp. 2369-2383
Author(s):  
Sharif M Ridwan ◽  
Ferris El-Tayyeb ◽  
James F Hainfeld ◽  
Henry M Smilowitz
Keyword(s):  
Radiation Therapy ◽  
Fluorescence Microscopy ◽  
Cell Surface ◽  
Tumor Therapy ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Confocal Fluorescence ◽  
Injection Materials ◽  
Over Time

Aim: To analyze the localization, distribution and effect of iodine nanoparticles (INPs) on radiation therapy (RT) in advanced intracerebral gliomas over time after intravenous injection. Materials & methods: Luciferase/td-tomato expressing U87 human glioma cells were implanted into mice which were injected intravenously with INPs. Mice with gliomas were followed for tumor progression and survival. Immune-stained mouse brain sections were examined and quantified by confocal fluorescence microscopy. Results: INPs injected intravenously 3 days prior to RT, compared with 1 day, showed greater association with CD31-staining structures, accumulated inside tumor cells more, covered more of the tumor cell surface and trended toward increased median survival. Conclusion: INP persistence and redistribution in tumors over time may enable greater RT enhancement and clinically relevant hypo-fractionated-RT and may enhance INP efficacy.

scholarly journals Hydrogen peroxide triggers an increase in cell surface expression of system xc− in cultured human glioma cells

2020 ◽  
Vol 134 ◽  
pp. 104648 ◽  
Author(s):  
Leah A. Chase ◽  
Mary VerHeulen Kleyn ◽  
NaTasha Schiller ◽  
Abby Goltz King ◽  
Guillermo Flores ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Surface ◽  
Glioma Cells ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Human Glioma ◽  
Human Glioma Cells

Liposomal transfection of human γ-interferon gene into human glioma cells and adoptive immunotherapy using lymphokine-activated killer cells

1994 ◽  
Vol 80 (3) ◽  
pp. 510-514 ◽  
Author(s):  
Masaaki Mizuno ◽  
Jun Yoshida ◽  
Toru Takaoka ◽  
Kenichiro Sugita
Keyword(s):  
Cell Surface ◽  
Growth Inhibition ◽  
Mhc Class I ◽  
Glioma Cell ◽  
Glioma Cells ◽  
Lak Cells ◽  
Class I ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Γ Interferon

✓ The authors evaluated the effect of liposomal transfection of human γ-interferon (HuIFN-γ) gene into human glioma cells and lymphokine-activated killer (LAK) cells, alone and in combination. An HuIFN-γ; gene inserted in a eukaryotic expression vector was entrapped in liposomes bearing positive surface charges. Liposomal gene transfection induced production of HuIFN-γ and its secretion in culture medium of human glioma cell lines (SK-MG-1 and U-251 MG). At 4 days after transfection, the cells produced 10 to 50 U/ml of HuIFN-γ in the medium, whereby the major histocompatibility complex (MHC) class I and II antigens, as well as intercellular adhesion molecule-1 (ICAM-1), were induced on the glioma cell surface. The growth-inhibiting effect of transfection-induced HuIFN-γ; was much stronger in comparison with control cultures exposed to 500 U/ml of exogenously added HuIFN-γ;. In addition, 20% to 40% growth inhibition was obtained in the glioma cells when they were treated with LAK cells alone at a 5:1 ratio of effector to target cells. Liposomal transfection of HulFN-γ; gene into human glioma cells combined with immunotherapy using LAK cells was more effective than either technique alone. The reinforcement of growth inhibition in the case of combined therapy was quenched by anti-ICAM-I monoclonal antibody, but not by anti-MHC class I or II monoclonal antibodies. These results suggest that the combined effect of liposomal transfection of HuIFN-γ; gene plus LAK cells into human glioma cells is a potentially useful therapy for malignant glioma, and that the mechanisms of the reinforcement of growth inhibition are closely related to the expression of ICAM-I on the glioma cell surface.

scholarly journals A narrow microbeam is more effective for tumor growth suppression than a wide microbeam: anin vivostudy using implanted human glioma cells

2011 ◽  
Vol 18 (4) ◽  
pp. 671-678 ◽  
Author(s):  
Atsushi Uyama ◽  
Takeshi Kondoh ◽  
Nobuteru Nariyama ◽  
Keiji Umetani ◽  
Manabu Fukumoto ◽  
...  
Keyword(s):  
Cell Death ◽  
Radiation Therapy ◽  
Tumor Growth ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Growth Ratio ◽  
Microbeam Irradiation ◽  
The Impact ◽  
Center Distance

The tumoricidal mechanisms of microbeam radiation therapy, and the more recently proposed minibeam radiation therapy, for the treatment of brain tumors are as yet unclear. Moreover, from among the various parameters of beam geometry the impact of changing the beam width is unknown. In this study, suppression of tumor growth in human glioma cells implanted in mice was evaluated experimentally using microbeams of two different widths: a conventional narrow beam (20 µm width, 100 µm center-to-center distance) and a wide beam (100 µm width, 500 µm center-to-center distance). The tumor growth ratio was compared and acute cell death was studied histologically. With cross-planar irradiation, tumor growth was significantly suppressed between days 4 and 28 after 20 µm microbeam irradiation, whereas tumor growth was suppressed, and not significantly so, only between days 4 and 18 after 100 µm microbeam irradiation. Immunohistochemistry using TUNEL staining showed no increase in TUNEL-positive cells with either microbeam at 24 and 72 h post-irradiation. The 20 µm microbeam was found to be more tumoricidal than the 100 µm microbeam, and the effect was not related to apoptotic cell death. The underlying mechanism may be functional tissue deterioration rather than direct cellular damage in the beam path.

Mutant IκBα Suppresses Hypoxia-Induced VEGF Expression Through Downregulation of HIF-1α and COX-2 in Human Glioma Cells

2005 ◽  
Vol 15 (3) ◽  
pp. 139-149 ◽  
Author(s):  
Yoshihira Kimba ◽  
Tatsuya Abe ◽  
Jian Liang Wu ◽  
Ryo Inoue ◽  
Minoru Fukiki ◽  
...  
Keyword(s):  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Vegf Expression ◽  
Cox 2

scholarly journals Differential expression and role of p21cip/waf1 and p27kip1 in TNF-α-induced inhibition of proliferation in human glioma cells

2007 ◽  
Vol 6 (1) ◽  
pp. 42 ◽  
Author(s):  
Pabbisetty Kumar ◽  
Anjali Shiras ◽  
Gowry Das ◽  
Jayashree C Jagtap ◽  
Vandna Prasad ◽  
...  
Keyword(s):  
Differential Expression ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Inhibition Of Proliferation ◽  
Tnf Α

scholarly journals Indirubin exerts anticancer effects on human glioma cells by inducing apoptosis and autophagy

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhaohui Li ◽  
Han Wang ◽  
Jun Wei ◽  
Liang Han ◽  
Zhigang Guo
Keyword(s):  
Apoptotic Cell ◽  
Treatment Strategies ◽  
Glioma Cells ◽  
Annexin V ◽  
Metastatic Potential ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Efficient Treatment ◽  
Cell Percentage ◽  
Anticancer Effects

Abstract Glioma causes significant mortality across the world and the most aggressive type of brain cancer. The incidence of glioma is believed to increase in the next few decades and hence more efficient treatment strategies need to be developed for management of glioma. Herein, we examined the anticancer effects of Indirubin against a panel of human glioma cells and attempted to explore the underlying mechanisms. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that Indirubin could inhibit the growth of all the glioma cells but the lowest IC50 of 12.5 µM was observed against the U87 and U118 glioma cells. Additionally, the cytotoxic effects of Indirubin were comparatively negligible against the normal astrocytes with an IC50 of > 100 µM. Investigation of mechanism of action, revealed that Indirubin exerts growth inhibitory effects on the U87 and U118 glioma cells by autophagic and apoptotic cell death. Annexin V/PI staining assay showed that apoptotic cell percentage increased dose dependently. Apoptosis was associated with increase in Bax decrease in Bcl-2 expressions. Additionally, the expression of autophagic proteins such as LC3II, ATG12, ATG15 and Beclin 1 was also increased. Wound heal assay showed that Indirubin caused remarkable decrease in the migration of the U87 and U118 cells indicative of anti-metastatic potential of Indirubin. Taken together, these results suggest that Indirubin exerts potent anticancer effects on glioma cells and may prove essential in the management of glioma.

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