Characterization of an umbilical cord blood sourced product suitable for allogeneic applications

Aim: Umbilical cord blood (UCB) sourced allografts are promising interventions for tissue regeneration. As applications of these allografts and regulations governing them continue to evolve, we were prompted to identify parameters determining their quality, safety and regenerative potential. Materials & methods: Flow-cytometry, mass-spectrometry, protein multiplexing, nanoparticle tracking analysis and standard biological techniques were employed. Results: Quality attributes of a uniquely processed UCB-allograft (UCBr) were enumerated based on identity (cell viability, immunophenotyping, proteomic profiling, and quantification of relevant cytokines); safety (bioburden and microbiological screening), purity (endotoxin levels) and potency (effect of UCBr on chondrocytes and mesenchymal stem cells derived exosomes). These attributes were stable up to 24 months in cryopreserved UCBr. Conclusion: We identified a comprehensive panel of tests to establish the clinical efficacy and quality control attributes of a UCB-sourced allograft.

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Quantification and Immunophenotypic Characterization of Bone Marrow and Umbilical Cord Blood Mesenchymal Stem Cells by Multicolor Flow Cytometry

Transplantation Proceedings ◽

10.1016/j.transproceed.2009.01.059 ◽

2009 ◽

Vol 41 (3) ◽

pp. 943-946 ◽

Cited By ~ 37

Author(s):

A.A. Martins ◽

A. Paiva ◽

J.M. Morgado ◽

A. Gomes ◽

M.L. Pais

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Flow Cytometry ◽

Mesenchymal Stem Cells ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Multicolor Flow Cytometry

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Therapeutic Potential of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

Current Stem Cell Research & Therapy ◽

10.2174/1574888x14666190214162453 ◽

2019 ◽

Vol 14 (6) ◽

pp. 460-465 ◽

Cited By ~ 3

Author(s):

Jing Jia ◽

Baitao Ma ◽

Shaoshuai Wang ◽

Ling Feng

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Tissue Regeneration ◽

Umbilical Cord Blood ◽

Therapeutic Potential ◽

Human Umbilical Cord Blood ◽

Proliferative Potential ◽

Human Umbilical Cord ◽

Preclinical Studies ◽

Endothelial Colony Forming Cells

Endothelial progenitor cells (EPCs) are implicated in multiple biologic processes such as vascular homeostasis, neovascularization and tissue regeneration, and tumor angiogenesis. A subtype of EPCs is referred to as endothelial colony-forming cells (ECFCs), which display robust clonal proliferative potential and can form durable and functional blood vessels in animal models. In this review, we provide a brief overview of EPCs’ characteristics, classification and origins, a summary of the progress in preclinical studies with regard to the therapeutic potential of human umbilical cord blood derived ECFCs (CB-ECFCs) for ischemia repair, tissue engineering and tumor, and highlight the necessity to select high proliferative CB-ECFCs and to optimize their recovery and expansion conditions.

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