One health in regenerative medicine: report on the second Havemeyer symposium on regenerative medicine in horses

Regenerative medicine is commonly used in human and equine athletes. Potential therapies include culture expanded stem cells, stromal vascular fraction of adipose tissue, platelet-rich plasma, bone marrow concentrate, or autologous conditioned serum. The purpose of this manuscript is to disseminate findings from a workshop on the development of translational regenerative medicine in the equine field. Five themes emerged: stem cell characterization and tenogenic differentiation; interactions between mesenchymal stem cells, other cells and the environment; scaffolds and cell packaging; blood- and bone marrow-based regenerative medicines; clinical use of regenerative therapies. Evidence gained through the use of regenerative medicine applications in the horse should continue to translate to the human patient, bringing novel regenerative therapies to both humans and horses.

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Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century

Stem Cells International ◽

10.1155/2015/734731 ◽

2015 ◽

Vol 2015 ◽

pp. 1-19 ◽

Cited By ~ 76

Author(s):

J.-F. Stoltz ◽

N. de Isla ◽

Y. P. Li ◽

D. Bensoussan ◽

L. Zhang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Regenerative Medicine ◽

Embryonic Stem ◽

Clinical Applications ◽

Cardiac Insufficiency ◽

Immunomodulatory Activity ◽

Hematopoietic Stem ◽

Fetal Stem Cells ◽

Organ Regeneration

Since the 1960s and the therapeutic use of hematopoietic stem cells of bone marrow origin, there has been an increasing interest in the study of undifferentiated progenitors that have the ability to proliferate and differentiate into various tissues. Stem cells (SC) with different potency can be isolated and characterised. Despite the promise of embryonic stem cells, in many cases, adult or even fetal stem cells provide a more interesting approach for clinical applications. It is undeniable that mesenchymal stem cells (MSC) from bone marrow, adipose tissue, or Wharton’s Jelly are of potential interest for clinical applications in regenerative medicine because they are easily available without ethical problems for their uses. During the last 10 years, these multipotent cells have generated considerable interest and have particularly been shown to escape to allogeneic immune response and be capable of immunomodulatory activity. These properties may be of a great interest for regenerative medicine. Different clinical applications are under study (cardiac insufficiency, atherosclerosis, stroke, bone and cartilage deterioration, diabetes, urology, liver, ophthalmology, and organ’s reconstruction). This review focuses mainly on tissue and organ regeneration using SC and in particular MSC.

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Effects of Porcine bone marrow-derived Platelet-Rich Plasma on bone marrow-derived mesenchymal stem cells and endothelial progenitor cells

Tissue and Cell ◽

10.1016/j.tice.2021.101587 ◽

2021 ◽

pp. 101587

Author(s):

Lei Shi ◽

Boon Ching Tee ◽

Zongyang Sun

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Platelet Rich Plasma ◽

Endothelial Progenitor

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What is the clinical applicability of regenerative therapies in dentistry?

RGO - Revista Gaúcha de Odontologia ◽

10.1590/1981-863720170002000113112 ◽

2017 ◽

Vol 65 (4) ◽

pp. 359-367 ◽

Cited By ~ 1

Author(s):

Giulia Tarquinio DEMARCO ◽

Laura Borges KIRSCHNICK ◽

Luis Bayardo WATSON ◽

Marcus Cristian MUNIZ CONDE ◽

Flávio Fernando DEMARCO ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Platelet Rich Plasma ◽

Blood Clot ◽

Collagen Scaffold ◽

Bone Tissue Regeneration ◽

Promising Alternative ◽

Organ Regeneration ◽

Regenerative Therapies

ABSTRACT Regenerative therapies have been widely developed in dentistry and it is important to incorporate dentists’ knowledge of these new therapies into the dental clinic routine. This study reviewed the literature on regenerative therapies and clinical applications. Tissue engineering has contributed to changes in the paradigm of restorative health sciences. Its pillars underpin the techniques of tissue and organ regeneration. Despite the majority of studies in this field being in vitro, a range of preclinical studies and methodologies has been formed using these principles and they are already being used on humans. The use of platelet-rich plasma and platelet-rich fibrin in surgery as natural scaffolds for the reestablishment of bone and periodontal tissue are often reported in the literature and clinical trials using this approach have shown promising results. Stem cells from autologous dental pulp have been successfully applied in bone tissue regeneration using natural collagen scaffold in humans. In addition, revascularization of the root canal already appears in the literature as a promising alternative to apexification. The principle behind this therapy is the use of the blood clot as a scaffold and the migration of stem cells of the apical papilla to regenerate the dental pulp organ. Final considerations: Although still in the early stages, regenerative therapies can now be used in dental practice. Knowledge of the principles governing these therapies should be understood by the dentist for use in clinical practice.

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Bone Marrow Stromal Stem Cells in Tissue Engineering and Regenerative Medicine

Hormone and Metabolic Research ◽

10.1055/s-0042-118458 ◽

2016 ◽

Vol 48 (11) ◽

pp. 700-713 ◽

Cited By ~ 57

Author(s):

A. Polymeri ◽

W. Giannobile ◽

D. Kaigler

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Bone Marrow ◽

Regenerative Medicine ◽

Stromal Stem Cells

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The Effect of Early Application of a Combined Therapy of Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Blood and Bone Parameters in Ovariectomized Rats

Journal of Evolutionary Biochemistry and Physiology ◽

10.1134/s0022093021040219 ◽

2021 ◽

Vol 57 (4) ◽

pp. 972-990

Author(s):

G. M. Hamed ◽

N. A. Nassef ◽

R. S. A. E. Mansour ◽

M. K. E. Shawky ◽

A. A. Abo Zeid ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Platelet Rich Plasma ◽

Combined Therapy ◽

Ovariectomized Rats ◽

Early Application ◽

Bone Parameters ◽

Marrow Mesenchymal Stem Cells

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Transplantation of Bone Marrow–Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Fibrin Glue for Periodontal Regeneration

The International Journal of Periodontics & Restorative Dentistry ◽

10.11607/prd.3691 ◽

2019 ◽

Vol 39 (1) ◽

pp. e32-e45 ◽

Cited By ~ 6

Author(s):

Mahdieh Rezaei ◽

Shahram Jamshidi ◽

Anna Saffarpour ◽

Mahdi Ashouri ◽

Reza Rahbarghazi ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Fibrin Glue ◽

Platelet Rich Plasma ◽

Periodontal Regeneration

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BMAC and Adipose-Derived MSCs Treatment for Knee Osteoarthritis: A Systematic Review

International Journal of Clinical Case Reports and Reviews ◽

10.31579/2690-4861/150 ◽

2021 ◽

Vol 7 (04) ◽

pp. 01-11

Author(s):

Pooja Pithadia

Keyword(s):

Systematic Review ◽

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Knee Osteoarthritis ◽

Bone Marrow Aspirate ◽

Stromal Vascular Fraction ◽

Cochrane Library ◽

Bone Marrow Aspirate Concentrate ◽

Mesh Terms

Background: Knee osteoarthritis is the most common musculoskeletal progressive disorder that affects nearly 303 million people worldwide. This condition prevails in 10% males and 13% females among the elders above 60. Although there is conventional nonsurgical and surgical treatment available for knee osteoarthritis, there is a fascinating interest in bone marrow aspirate concentrate (BMAC) as well as adipose-derived mesenchymal stem cells (AD-MSC), including enzymatically treated stromal vascular fraction (SVF) and mechanically treated (microfat/nanofat) injections among physicians. Hence, this systematic review aims to determine the efficacy of BMAC and AD-MSCs (enzyme and mechanically treated) injections for knee osteoarthritis treatment. Methods: A systematic review was performed on the following data sources (PubMed, Scopus, Google Scholar, EMBASE, and Cochrane Library) published on March 31, 2021. The keywords or MeSH terms include 'Knee Osteoarthritis with 'Bone marrow aspirate concentrate' OR 'BMAC' or with 'Adipose-derived mesenchymal stem cells (AD-MSC)' or with 'Stromal vascular fraction' OR 'SVF' or 'Mechanically treated AD-MSC (mfat/nanofat)'. In addition, the retrieved articles were further reviewed to identify relevant research studies. Results: The authors reviewed and tabulated data based on the year of study, study type, therapy protocol, patient population, outcome measures, and interpretation. Among the 382 records screened, 43 studies (16 on BMAC and 27 on AD-MSCs) were included in the systematic review study. Among them, only 5 were randomized controlled trials. These selected studies demonstrated short-term positive outcomes such as improvement in knee pain and function with no adverse side effects. Moreover, researchers reported varied administration methods of BMAC or AD-MSC either as standalone or in combination with other conservative procedures such as PRP (Platelets Rich Plasma), HA (Hyaluronic acid), or surgery. Conclusions: BMAC and AD-MSC (enzymatically and mechanically treated) injections prove safer and more efficacious in patients with knee osteoarthritis for a shorter duration of 2 years. However, the available literature lacks high-quality studies with no varied clinical settings and long-term follow-up of more than two years.

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