The tissue-specific and developmental expression
patterns of the forkhead transcription factor FoxO1
gene in pigs

The Caenorhabditis elegans gene pes-1 encodes a transcription factor of the forkhead family and is expressed in specific cells of the early embryo. Despite these observations suggesting pes-1 to have an important regulatory role in embryogenesis, inactivation of pes-1 caused no apparent phenotype. This lack of phenotype is a consequence of genetic redundancy. Whereas a weak, transitory effect was observed upon disruption of just T14G12.4 (renamed fkh-2) gene function, simultaneous disruption of the activity of both fkh-2 and pes-1 resulted in a penetrant lethal phenotype. Sequence comparison suggests these two forkhead genes are not closely related and the functional association of fkh-2 and pes-1 was only explored because of the similarity of their expression patterns. Conservation of the fkh-2/pes-1 genetic redundancy between C. elegans and the related species C. briggsae was demonstrated. Interestingly the redundancy in C. briggsae is not as complete as in C. elegans and this could be explained by alterations of pes-1 specific to the C. briggsae ancestry. With overlapping function retained on an evolutionary time-scale, genetic redundancy may be extensive and expression pattern data could, as here, have a crucial role in characterization of developmental processes.

Download Full-text

Tissue-specific expression patterns of nuclear receptors

NURSA Datasets ◽

10.1621/datasets.02001 ◽

2013 ◽

Author(s):

AL Bookout ◽

Y Jeong ◽

M Downes ◽

RT Yu ◽

RM Evans ◽

...

Keyword(s):

Nuclear Receptors ◽

Expression Patterns ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression

Download Full-text

Faculty Opinions recommendation of Asymmetric arginine dimethylation determines life span in C. elegans by regulating forkhead transcription factor DAF-16.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.11326959.14375054 ◽

2011 ◽

Author(s):

Pankaj Kapahi

Keyword(s):

Transcription Factor ◽

Life Span ◽

Forkhead Transcription Factor ◽

C Elegans

Download Full-text

Tissue-specific developmental expression of the kallikrein gene family in the rat.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)40160-9 ◽

1990 ◽

Vol 265 (2) ◽

pp. 1077-1081

Author(s):

J A Clements ◽

B A Matheson ◽

J W Funder

Keyword(s):

Gene Family ◽

Developmental Expression ◽

Tissue Specific

Download Full-text

Syntaxin 1A Gene Is Negatively Regulated in a Cell/Tissue Specific Manner by YY1 Transcription Factor, Which Binds to the −183 to −137 Promoter Region Together with Gene Silencing Factors Including Histone Deacetylase

Biomolecules ◽

10.3390/biom11020146 ◽

2021 ◽

Vol 11 (2) ◽

pp. 146

Author(s):

Takahiro Nakayama ◽

Toshiyuki Fukutomi ◽

Yasuo Terao ◽

Kimio Akagawa

Keyword(s):

Transcription Factor ◽

Gene Silencing ◽

Protein Complex ◽

Promoter Region ◽

Neuronal Cell ◽

Syntaxin 1A ◽

Tissue Specific ◽

Cell Tissue ◽

Specific Manner ◽

A Cell

The HPC-1/syntaxin 1A (Stx1a) gene, which is involved in synaptic transmission and neurodevelopmental disorders, is a TATA-less gene with several transcription start sites. It is activated by the binding of Sp1 and acetylated histone H3 to the −204 to +2 core promoter region (CPR) in neuronal cell/tissue. Furthermore, it is depressed by the association of class 1 histone deacetylases (HDACs) to Stx1a–CPR in non-neuronal cell/tissue. To further clarify the factors characterizing Stx1a gene silencing in non-neuronal cell/tissue not expressing Stx1a, we attempted to identify the promoter region forming DNA–protein complex only in non-neuronal cells. Electrophoresis mobility shift assays (EMSA) demonstrated that the −183 to −137 OL2 promoter region forms DNA–protein complex only in non-neuronal fetal rat skin keratinocyte (FRSK) cells which do not express Stx1a. Furthermore, the Yin-Yang 1 (YY1) transcription factor binds to the −183 to −137 promoter region of Stx1a in FRSK cells, as shown by competitive EMSA and supershift assay. Chromatin immunoprecipitation assay revealed that YY1 in vivo associates to Stx1a–CPR in cell/tissue not expressing Stx1a and that trichostatin A treatment in FRSK cells decreases the high-level association of YY1 to Stx1a-CPR in default. Reporter assay indicated that YY1 negatively regulates Stx1a transcription. Finally, mass spectrometry analysis showed that gene silencing factors, including HDAC1, associate onto the −183 to −137 promoter region together with YY1. The current study is the first to report that Stx1a transcription is negatively regulated in a cell/tissue-specific manner by YY1 transcription factor, which binds to the −183 to −137 promoter region together with gene silencing factors, including HDAC.

Download Full-text