scholarly journals Pengaruh NAA dan BAP terhadap Kultur Jaringan Nenas Tangkit (Ananas comosus (L.) Merr. cv. Tangkit)

Biospecies ◽  
2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Zulkarnain ZULKARNAIN ◽  
Neliyati NELIYATI
Keyword(s):  
Shoot Growth ◽  
Plant Biotechnology ◽  
Shoot Formation ◽  
Ananas Comosus ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Formation Number ◽  
Agricultural Faculty ◽  
Time Required

This study was aimed at investigating the effect of NAA and BAP on the development of basal slip slices in tissue culture of pineapple cv. Tangkit. The experiment was conducted at the Plant Biotechnology Laboratory Agricultural Faculty University of Jambi from July through to October 2016. Six level of NAA concentrations (0, 1, 2, 3 , 4 and 5 mgL-1) were tested in combination with six levels of BAP concentration (0, 1, 2, 3 , 4 and 5 mgL-1). A Completely Randomized Design with 5 replicates was employed in this trial. Data on the percentage of explants forming shoots, time required for shoot formation, number of shoots growing per explants, and callus proliferation were recorded. Results showed that without the involvement of plant growth regulators there had been no growth on cultured explants. However, shoot growth was found on explants cultured on medium supplemented with 1 – 3 mgL-1 NAA or 1 – 5 mgL-1 BAP. The application of NAA higher than 3 mgL-1 was found to suppress shoot growth even in combination with BAP. Shoot formation was found to be fast on medium supplemented with 1 mgL-1 NAA + 4 mgL-1 BAP, but took longer time on medium with or 1 mgL-1 NAA + 5 mgL-1 BAP as well as medium with 1 – 2 mgL-1 NAA only. The same phenomenon was also found on medium with 1 – 2 mgL-1 NAA only. This study also revealed that most of cultured explants regenerated only one shoot, but explants cultured on medium with 1 mgL-1 NAA along with 1 mgL-1 BAP produced 2.33 shoots on the average. The addition of 1 mgL-1 NAA without BAP and 1 mgL-1 BAP without NAA could increase average shoot growth by 1.78 and 1.44, respectively. Keywords:   in vitro culture, micropropagation, naphthalene acetic acid, benzyl amino purine.      

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro

2012 ◽  
Vol 10 (2) ◽  
pp. 54-60
Author(s):  
IRMA PUTRI HAYANTI ◽  
RETNA BANDRIYATI AMIPUTRI ◽  
PRASWANTO PRASWANTO
Keyword(s):  
Shoot Growth ◽  
Organic Fertilizer ◽  
Centella Asiatica ◽  
Culture Method ◽  
Shoot Formation ◽  
Randomized Design ◽  
Leaf Formation ◽  
Medicinal Crop ◽  
Completely Randomized Design

Hayanti IP, Amiputri RB, Praswanto. 2012. The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro. Biofarmasi 10: 54-60. Gotu kola (Centella asiatica L.) is a medicinal crop, needed by local industry of pharmacy. Gotu kola in Indonesia is not enough to supply in market requisites. Tissue culture method was used to multiply gotu kola with a combination of medium from liquid organic fertilizer and pearl grass extract. The purposes of the research were to determine the effect of liquid organic fertilizer and pearl grass extract on the shoot growth of gotu kola in vitro, and to obtain the optimal concentration of liquid organic fertilizer and pearl grass extract combination on the shoot formation of gotu kola in vitro. The research was conducted in November 2010 to June 2011 in Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, Surakarta. The experimental design used was a Completely Randomized Design (CRD) with two treatment factors and three replications. The first factor was liquid organic fertilizer concentrations, i.e. 0 mL/L, 2 mL/L, 4 mL/L and 8 mL/L. The second factor was pearl grass extract, i.e. 0 mL/L, 3 mL/L, 6 mL/L and 12 mL/L. Variables observed were the percentage of shoot formation, the time of shoot formation, the number of shoot, the length of shoot, the percentage of leaf formation, the time of leaf formation and the number of leaf. The result of the research showed that not all of treatments provided shoot formation. The combination of liquid organic fertilizer concentrations 0 mL/L and pearl grass extract 12 mL/L showed the best results, in which the average of length of shoot was 2.16 cm, the number of shoot was 8 and the number of leaf was 8.

scholarly journals Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas

2021 ◽  
Vol 4 (1) ◽  
pp. 82-92
Author(s):  
Rustikawati Rustikawati ◽  
Catur Herison ◽  
Entang Inoriah ◽  
Vera Dwisari
Keyword(s):  
Shoot Growth ◽  
Shoot Formation ◽  
Experimental Unit ◽  
Shoot Height ◽  
Weight Percentage ◽  
Randomized Design ◽  
Significant Difference ◽  
Long Time ◽  
Planting Materials

Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation.  The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design.  The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm.  The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.).  Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud.  Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color.  Mean comparison was performed by the Least Significant Difference (LSD).  The results showed that there was no interaction between BAP concentration and genotype on any variable observed.  The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro.  The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.

scholarly journals INDUKSI TUNAS JERUK SIAM DENGAN PENAMBAHAN BENZIL AMINO PURINE (BAP) SECARA IN VITRO

2019 ◽  
Vol 9 (2) ◽  
pp. 50-55
Author(s):  
Yulianti Rasud ◽  
Hairil Anwar
Keyword(s):  
Plant Biotechnology ◽  
Shoot Formation ◽  
Medium Composition ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Leaf Formation ◽  
Difference Test ◽  
Medium Culture

The aims of this experiments was to evaluate the appropriate concentration of BAP for shoots induction of orange via in vitro culture. This experiment was conducted in Plant Biotechnology Laboratory, Faculty of Agriculture, Tadulako University from Mei to August 2017.  This experiment used a Completely Randomized Design with five treatments, namely 0.5 ppm BAP, 1.0 ppm BAP, 1.5 ppm BAP, 2.0 ppm and 2.5 ppm..  Data was analyzed using analysis of variance and differences between means of the treatments were determined by Honestly Significant Difference test at 5% level.  Results of this experiment indicated that the most suitable concentration of BAP for the shoots induction of orange was the medium culture supplemented with 0.5 ppm BAP. In such medium composition, the quickest and the highest number of shoot formation was obtained as well as the highest number of leaf formation at 6 weeks after culture, namely 3.02 days after culture, 1.52 shoots and 2.40 leaves per explant, respectively.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals Callus Induction and Shoot Formation for Mexican Red Bean (Phaseolus vulgaris L.) Pinto Cultivar in Vitro

2020 ◽  
pp. 1887-1893
Author(s):  
Rasha K. Mohammed Al-Saedi ◽  
Ansam G. Abdulhalem
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Phaseolus Vulgaris L ◽  
Different Types ◽  
Red Bean ◽  
Internode Explants

     The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as  internodes, cotyledons and roots,  were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.

scholarly journals PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

2016 ◽  
Vol 31 (1) ◽  
pp. 33
Author(s):  
Sri Hartati ◽  
Agus Budiyono ◽  
Ongko Cahyono
Keyword(s):  
Plant Conservation ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Significant Difference ◽  
Two Factors ◽  
Number Of Leaves ◽  
Media Research ◽  
Test Level ◽  
Range Test

<p><em>Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of  </em><em>hybrids</em><em> orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. </em><em>The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor  was the type media of </em><em>Benzyl Amino Purine</em><em> /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). </em><em>Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that </em><em>the addition  </em><em>of NAA 3 ppm </em><em>as much as 4,96 cm </em><em> and BAP 3 ppm </em><em>as much as 4,41 cm </em><em> give a significant effect on the increase of high plantlets</em><em> </em><em>and  so NAA 3 ppm </em><em>as much as 5,76 cm </em><em> effect  on  roots length , but did not significantly effect the number of leaves and roots.</em></p>

scholarly journals In Vitro Growth of Sunflower (Helianthus annuus) via Direct Organogenesis

2021 ◽  
pp. 60-64
Keyword(s):  
Helianthus Annuus ◽  
Shoot Growth ◽  
Seed Oil ◽  
Fungal Growth ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Sunflower Seeds ◽  
Micro Propagation ◽  
Regeneration Efficiency

Sunflower (Helianthus annuus) is a crop of increasing importance as a source of seed oil and proteins; nonetheless, the number of studies on sunflower tissue culture is somewhat limited. The development of a competent in vitro direct organogenesis protocol involves important basic steps of regeneration. In our study, chemically sterilized sunflower seeds were planted on induction media, and 52.54 % germination efficiency was found. While the seeds were subjected to regeneration containing 2 mg/L of cytokinin, Benzyl Adenopurine (BAP) as well as 1 mg/L of auxin, Naphthalene Acetic Acid (NAA); shoot growth was observed with41 % regeneration efficiency. Non-sterilized seeds germinated but showed fungal growth on the surface of media resulting in no regeneration of sunflower plantlet. On the other hand, sterile seeds germinated less with little or no fungal growth leading to successful regeneration. Frequent regeneration of sterile sunflower seeds through direct organogenesis can contribute to enhanced micro-propagation of this plant.

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