scholarly journals Bio-safety assessment of nanozeolites of varying size and doses on soil beneficial microorganisms

2021 ◽  
Vol 42 (4(SI)) ◽  
pp. 1181-1190
Author(s):  
L. Sivashankari ◽  
◽  
S.K. Rajkishore ◽  
A. Lakshmanan ◽  
K.S. Subramanian ◽  
...  

Aim: To evaluate the toxic effects of nano-zeolites on soil beneficial microorganisms. Methodology: This study involved dry milling of zeolites at varying parameters to obtain dry-milled zeolites of four size regimes such as 10-100 nm, 200-400 nm, 500-1000 nm and 1000-2000 nm that were tested at incremental concentrations such as 100 ppm, 1000 ppm and 2000 ppm along with control on various soil beneficial microorganisms. Three replications were done for each treatment with factorial completely randomized design. The poison food technique, growth curve assessment followed by cytotoxicity and genotoxicity studies on all four bacterial genera treated with zeolites of varying sizes and doses were undertaken. The effects of zeolite on average linear growth rate (ALGR) of biocontrol agent, Trichoderma viride were also studied. Results: The effect of zeolites tested on four bacterial genera viz. Azotobacter chroococcum, Rhizobium leguminosarum, Bacillus megaterium and Pseudomonas fluorescens using poison food technique and growth curve revealed that zeolites regardless of size or concentration had positively influenced the growth dynamics of all four bacteria tested. The effect of zeolite on average linear growth rate (ALGR) of Trichoderma viride also indicated that incremental dose of zeolite had a positive effect. Lactose dehydrogenase revealed that 2000 ppm nano-zeolite exhibited cytotoxic effects on soil beneficial micro-organisms tested. On the other hand, comet assay demonstrated no quantifiable DNA damage in nano-zeolite treated cells in comparison to control cultures. Interpretation: This study unequivocally demonstrated that zeolites of size greater than 200-400 nm, irrespective of doses even up to 2000 ppm are quite safe for soil beneficial microbes.

1993 ◽  
Vol 67 (6) ◽  
pp. 922-934 ◽  
Author(s):  
Robert J. Elias ◽  
Dong-Jin Lee

Microborings in the Late Ordovician tabulate corals Catenipora rubra (a halysitid) and Manipora amicarum (a cateniform nonhalysitid) and in an epizoic solitary rugose coral differ from nearly all of those previously reported in Paleozoic corals. These microborings were formed within the coralla by endolithic algae and fungi located beneath living polyps. Comparable structures in the Late Ordovician tabulate Quepora ?agglomeratiformis (a halysitid) represent algal microborings, not spicules, and halysitids are corals, not sponges as suggested by Kaźmierczak (1989).Endolithic algae in cateniform tabulates relied primarily on light entering through the outer walls of the ranks rather than through the polyps; lacunae within coralla permitted appropriate levels of light to reach many corallites. The direction of boring was determined by corallum microstructure and possibly also by the distribution of organic matter within the skeleton. There is an apparent inverse correlation between boring activity and coral growth rate.The location and relative abundance of pyritized microborings within calcareous coralla can be established quantitatively and objectively from electron microprobe determinations of weight percent sulfur along appropriate traverses of the coral skeleton. The distribution of such microborings in Catenipora rubra and Manipora amicarum is comparable to algal banding in modern corals; this is the first report of such banding in the interiors of Paleozoic corals. Change in the intensity of boring within each corallum was evidently a response to variation in the linear growth rate of the coral, or to fluctuation in an environmental factor (perhaps light intensity) that could control both algal activity and growth rate in these corals. Change in the algal boring intensity and linear growth rate of the coral was generally but not always seasonal and usually but not invariably associated with change in the density of coral skeletal deposition.Cyclic bands of boring abundance maxima within fossil colonial corals provide a measure of annual linear growth comparable to the widely accepted method based on skeletal density bands. Algal bands are more sporadically developed than density bands within and among coralla, thus increasing the difficulty of interpretation. Fluctuations in the abundance of algal microborings apparently provide a detailed record of changes in the linear growth rate of colonies and of individuals within colonies. Combined analyses of microboring abundance and skeletal density will contribute significantly to our understanding of the biological and environmental factors involved in endolithic activity and coral growth.


2011 ◽  
Vol 8 (6) ◽  
pp. 12247-12283
Author(s):  
P. Sabatier ◽  
J.-L. Reyss ◽  
J. M. Hall-Spencer ◽  
C. Colin ◽  
N. Frank ◽  
...  

Abstract. Here we show the use of the 210Pb-226Ra excess method to determine the growth rate of corals from one of the world's largest known cold-water coral reef, the Røst Reef off Norway. Two large branching framework-forming cold-water coral specimens, one Lophelia pertusa and one Madrepora oculata were collected alive at 350 m water depth from the Røst Reef at ~67° N and ~9° E. Pb and Ra isotopes were measured along the major growth axis of both specimens using low level alpha and gamma spectrometry and the corals trace element compositions were studied using ICP-QMS. Due to the different chemical behaviors of Pb and Ra in the marine environment, 210Pb and 226Ra were not incorporated the same way into the aragonite skeleton of those two cold-water corals. Thus to assess of the growth rates of both specimens we have here taken in consideration the exponential decrease of initially incorporated 210Pb as well as the ingrowth of 210Pb from the decay of 226Ra. Moreover a~post-depositional 210Pb incorporation is found in relation to the Mn-Fe coatings that could not be entirely removed from the oldest parts of the skeletons. The 226Ra activities in both corals were fairly constant, then assuming constant uptake of 210Pb through time the 210Pb-226Ra chronology can be applied to calculate linear growth rate. The 45.5 cm long branch of M. oculata reveals an age of 31 yr and a~linear growth rate of 14.4 ± 1.1 mm yr−1, i.e. 2.6 polyps per year. However, a correction regarding a remaining post-depositional Mn-Fe oxide coating is needed for the base of the specimen. The corrected age tend to confirm the radiocarbon derived basal age of 40 yr (using 14C bomb peak) with a mean growth rate of 2 polyps yr−1. This rate is similar to the one obtained in Aquaria experiments under optimal growth conditions. For the 80 cm-long specimen of L. pertusa a remaining contamination of metal-oxides is observed for the middle and basal part of the coral skeleton, inhibiting similar accurate age and growth rate estimates. However, the youngest branch was free of Mn enrichment and this 15 cm section reveals a growth rate of 8 mm yr−1 (~1 polyp every two to three years). However, the 210Pb growth rate estimate is within the lowermost ranges of previous growth rate estimates and may thus reflect that the coral was not developing at optimal growth conditions. Overall, 210Pb-226Ra dating can be successfully applied to determine the age and growth rate of framework-forming cold-water corals, however, removal of post-depositional Mn-Fe oxide deposits is a prerequisite. If successful, large branching M. oculata and L. pertusa coral skeletons provide unique oceanographic archive for studies of intermediate water environmentals with an up to annual time resolution and spanning over many decades.


Fluids ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 39 ◽  
Author(s):  
Roger Grimshaw

The linear stability theory of wind-wave generation is revisited with an emphasis on the generation of wave groups. The outcome is the fundamental requirement that the group move with a real-valued group velocity. This implies that both the wave frequency and the wavenumber should be complex-valued, and in turn this then leads to a growth rate in the reference frame moving with the group velocity which is in general different from the temporal growth rate. In the weakly nonlinear regime, the amplitude envelope of the wave group is governed by a forced nonlinear Schrödinger equation. The effect of the wind forcing term is to enhance modulation instability both in terms of the wave growth and in terms of the domain of instability in the modulation wavenumber space. Also, the soliton solution for the wave envelope grows in amplitude at twice the linear growth rate.


2007 ◽  
Vol 25 (6) ◽  
pp. 1311-1322 ◽  
Author(s):  
R. B. Cosgrove

Abstract. It has recently been shown, by computation of the linear growth rate, that midlatitude sporadic-E (Es) layers are subject to a large scale electrodynamic instability. This instability is a logical candidate to explain certain frontal structuring events, and polarization electric fields, which have been observed in Es layers by ionosondes, by coherent scatter radars, and by rockets. However, the original growth rate derivation assumed an infinitely thin Es layer, and therefore did not address the short wavelength cutoff. Also, the same derivation ignored the effects of F region loading, which is a significant wavelength dependent effect. Herein is given a generalized derivation that remedies both these short comings, and thereby allows a computation of the wavelength dependence of the linear growth rate, as well as computations of various threshold conditions. The wavelength dependence of the linear growth rate is compared with observed periodicities, and the role of the zeroth order meridional wind is explored. A three-dimensional paper model is used to explain the instability geometry, which has been defined formally in previous works.


Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 239 ◽  
Author(s):  
Aikaterini Papadaki ◽  
Panagiota Diamantopoulou ◽  
Seraphim Papanikolaou ◽  
Antonios Philippoussis

Morchella sp. is one of the most expensive mushrooms with a high nutritional profile. In this study, the polysaccharide content of Morchella species was investigated. Specifically, mycelium growth rate, biomass production, sclerotia formation, and glucosamine and total polysaccharides content of six Morchella species grown on a starch-based media were evaluated. Submerged fermentations in potato dextrose broth resulted in a glucosamine content of around 3.0%. In solid-state fermentations (SSF), using potato dextrose agar, a high linear growth rate (20.6 mm/day) was determined. Increased glucosamine and total polysaccharides content were observed after the formation of sclerotia. Biomass and glucosamine content were correlated, and the equations were used for the indirect estimation of biomass in SSF with agro-industrial starch-based materials. Wheat grains (WG), potato peels (PP), and a mixture of 1:1 of them (WG–PP) were evaluated as substrates. Results showed that the highest growth rate of 9.05 mm/day was determined on WG and the maximum biomass yield (407 mg/g) on WG–PP. The total polysaccharide content reached up to 18.4% of dried biomass in WG–PP. The results of the present study proved encouraging for the efficient bioconversion of potato and other starch-based agro-industrial waste streams to morel biomass and sclerotia eliciting nutritional and bioactive value.


1996 ◽  
Vol 270 (1) ◽  
pp. R246-R253 ◽  
Author(s):  
D. W. Boyle ◽  
S. Lecklitner ◽  
E. A. Liechty

The purpose of the present investigation was to study the effect of 7 days of uterine blood flow reduction on fetal growth. Reduction in uterine blood flow was accomplished by external occlusion of the terminal aorta in 20 pregnant sheep. Linear growth was monitored daily by means of a crown-rump length measuring device. The deliveries of oxygen, glucose, and lactate to the fetus, as well as their uptakes by the fetus, were determined before and after 7 days of uterine blood flow reduction and correlated with rates of fetal growth. Identical studies were conducted in nine control animals. Uterine blood flow reduction resulted in a significant decrease in fetal oxygen delivery and fetal arterial oxygen content. Linear growth rate decreased by 38% in the occluded animals during hypoxemia. In addition, there was a 20% reduction in daily weight gain in occluded animals compared with controls. There were no differences in the uptakes of oxygen, glucose, and lactate by the fetus. Positive correlations were found between linear growth rate and fetal arterial oxygen content (r2 = 0.25, P = 0.0001) and between linear growth rate and fetal oxygen delivery (r2 = 0.21, P = 0.0006). The correlations between linear growth rate and fetal oxygenation provide strong evidence of the central role of oxygen in the regulation of fetal growth.


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