Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

We developed mid-infrared optoacoustic microscopy (MiROM), a bond-selective imaging modality that overcomes water/tissue opacity and depth limitations of mid-infrared sensing allowing uncompromised live-cell/thick-tissue mid-infrared microscopy with up to three orders of magnitudehigher sensitivity than other vibrational imaging modalities; such as Raman. We showcase the functional label-free biomolecular imaging capabilities of MiROM by monitoring the spatiotemporal dynamics of lipids and proteins during lipolysis in living adipocytes. Since MiROM, contrary to Ramanmodalities, is not only able to detect lipids and proteins, but also important metabolites such as glucose without the need of labels, here we discuss how MiROM yields novel functional label-free abilities for a broader range of analytical studies in living cells and tissues.

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Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

Nature Protocols ◽

10.1038/nprot.2011.386 ◽

2011 ◽

Vol 6 (11) ◽

pp. 1748-1760 ◽

Cited By ~ 111

Author(s):

Ralf Schröder ◽

Johannes Schmidt ◽

Stefanie Blättermann ◽

Lucas Peters ◽

Nicole Janssen ◽

...

Keyword(s):

G Protein ◽

Living Cells ◽

G Protein Coupled Receptors ◽

Label Free ◽

Dynamic Mass ◽

Mass Redistribution ◽

G Protein Coupled ◽

Free Dynamic

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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Journal of Visualized Experiments ◽

10.3791/58486 ◽

2018 ◽

Cited By ~ 5

Author(s):

André Gemeinhardt ◽

Matthew P. McDonald ◽

Katharina König ◽

Michael Aigner ◽

Andreas Mackensen ◽

...

Keyword(s):

Living Cells ◽

Label Free

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Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽

10.3390/bios8040102 ◽

2018 ◽

Vol 8 (4) ◽

pp. 102 ◽

Cited By ~ 8

Author(s):

Richard Schasfoort ◽

Fikri Abali ◽

Ivan Stojanovic ◽

Gestur Vidarsson ◽

Leon Terstappen

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Mammalian Cells ◽

Living Cells ◽

Label Free ◽

Intact Cells ◽

Cell Parameters ◽

Spr Imaging ◽

Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

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Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

10.1101/289504 ◽

2018 ◽

Cited By ~ 6

Author(s):

Chawin Ounkomol ◽

Sharmishtaa Seshamani ◽

Mary M. Maleckar ◽

Forrest Collman ◽

Gregory R. Johnson

Keyword(s):

Fluorescence Microscopy ◽

Three Dimensional ◽

Living Cells ◽

Transmitted Light ◽

Integrated Systems ◽

Label Free ◽

Subcellular Structure ◽

Modern Biology ◽

Transmitted Light Microscopy ◽

Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

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Methods and applications of label-free cell-based systems

KnE Engineering ◽

10.18502/keg.v0i0.493 ◽

2016 ◽

Vol 1 (1) ◽

Author(s):

Joachim Wiest

Keyword(s):

Environmental Monitoring ◽

Morphological Changes ◽

Free Cell ◽

Living Cells ◽

Label Free ◽

Controlled System ◽

Chip Technology ◽

Cell Monitoring ◽

A Cell ◽

On Chip

Label-free monitoring of living cells is used in various applications such as drug development, toxicology, regenerative medicine or environmental monitoring. The most prominent methods for monitoring the extracellular acidification, oxygen consumption, electrophysiological activity and morphological changes of living cells are described. Furthermore, the intelligent mobile lab (IMOLA) – a computer controlled system integrating cell monitoring and automated cell cultivation – is described as an example of a cell-based system for microphysiometry. Results from experiments in the field of environmental monitoring using algae are presented. An outlook toward the development of an organ-on-chip technology is given.

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Label-free, High-Resolution Optical Metabolic Imaging of Human Cervical Precancers Reveals Potential for Intraepithelial Neoplasia Diagnosis

Cell Reports Medicine ◽

10.1016/j.xcrm.2020.100017 ◽

2020 ◽

Vol 1 (2) ◽

pp. 100017

Author(s):

Dimitra Pouli ◽

Hong-Thao Thieu ◽

Elizabeth M. Genega ◽

Laura Baecher-Lind ◽

Michael House ◽

...

Keyword(s):

High Resolution ◽

Intraepithelial Neoplasia ◽

Metabolic Imaging ◽

Label Free

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Graphene-Based Biosensors for Detection of Composite Vibrational Fingerprints in the Mid-Infrared Region

Nanomaterials ◽

10.3390/nano9101496 ◽

2019 ◽

Vol 9 (10) ◽

pp. 1496

Author(s):

Yijun Cai ◽

Yanfen Hang ◽

Yuanguo Zhou ◽

Jinfeng Zhu ◽

Jingwen Yang ◽

...

Keyword(s):

Limit Of Detection ◽

Graphene Nanoribbons ◽

Infrared Region ◽

Infrared Range ◽

Label Free ◽

Mid Infrared ◽

Performance Limit ◽

Wide Range ◽

Vibrational Signals ◽

Physical Insight

In this study, a label-free multi-resonant graphene-based biosensor with periodic graphene nanoribbons is proposed for detection of composite vibrational fingerprints in the mid-infrared range. The multiple vibrational signals of biomolecules are simultaneously enhanced and detected by different resonances in the transmission spectrum. Each of the transmission dips can be independently tuned by altering the gating voltage applied on the corresponding graphene nanoribbon. Geometric parameters are investigated and optimized to obtain excellent sensing performance. Limit of detection is also evaluated in an approximation way. Besides, the biosensor can operate in a wide range of incident angles. Electric field intensity distributions are depicted to reveal the physical insight. Moreover, another biosensor based on periodic graphene nanodisks is further proposed, whose performance is insensitive to the polarization of incidence. Our research may have a potential for designing graphene-based biosensor used in many promising bioanalytical and pharmaceutical applications.

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