scholarly journals Indicadores bacterianos no habituales de la calidad de aguas naturales

2013 ◽  
Vol 5 (2) ◽  
pp. 283-287
Author(s):  
Sara Ávila de Navia ◽  
Sandra Mónica Estupiñán Torres ◽  
Flor Helena Chavarro ◽  
Diana Alejandra Acero
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Aeromonas Hydrophila ◽  
Pseudomonas Spp

Aeromonas spp. y Pseudomonas spp. están presentes en el agua y han sido propuestos como indicadores de su estado trófico, son un riesgo para la salud pública debido a que son patógenos oportunistas. La normativa colombiana no contempla a estos géneros microbianos como indicadores de la calidad microbiológica del agua. Este estudio busca enriquecer la información que sustente la inclusión de Aeromonas spp.y Pseudomonas spp, como indicadores en las Normas de la calidad microbiológica del agua en Colombia. Se evaluaron once puntos de las fuentes de agua del parque Natural Chicaque en época de lluvias (Noviembre del 2010) y época seca (Agosto del 2011). Se utilizó la técnica de filtración por membrana, y la identificación se realizó a través de pruebas bioquímicas rápidas Crystal. Se identificaron en las dos temporadas Pseudomonas spp. y Aeromonas spp, en particular Pseudomonas aeruginosa y Aeromonas hydrophila y otros Bacilos Gram Negativos. En época de lluvias se encontró un menor recuento de Pseudomonas spp., mientras que el recuento de Aeromonas spp. fue mayor en ésta misma época. Se sugiere continuar realizando más estudios que sustenten su inclusión como indicadores en las normas de la calidad microbiológica de agua en Colombia.PALABRAS CLAVEPseudomonas, Aeromonas, calidad del agua, microorganismos, filtración por membrana, calidad microbiológica

scholarly journals Characterisation of the Prevailing Multidrug Pseudomonas aeruginosa Strains from Surgical Wound Using 16S rRNA Sequencing Technique

2021 ◽  
Vol 28 (4) ◽  
pp. 37-49
Author(s):  
Eremwanarue Osagie Aibuedefe ◽  
Nwawuba Stanley Udogadi ◽  
Shittu Olalekan Hakeem
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Wound Infections ◽  
Surgical Wound ◽  
Specific Primer ◽  
Pseudomonas Spp ◽  
Multiple Drug Resistant ◽  
Rrna Sequencing

Background: Pseudomonas aeruginosa (P. aeruginosa) is prevalent in hospital-acquired surgical wound infections. It exhibits both innate and acquired resistance to a broad range of antimicrobials and remains a principal problem in clinical practice. Methods: In total, 284 sterile surgical wound swabs (142 each) were collected from two government hospitals: Central Hospital Benin (CHB) and University of Benin Teaching Hospital (UBTH) in Benin City, Nigeria. Pseudomonas spp. isolated from both hospitals were screened with eight different antibiotics by way of disk diffusion method. Polymerase chain reaction (PCR) amplification of 34 multiple drug-resistant isolates was carried out using genus-specific primer set on extracted genomic DNA for the identification of Pseudomonas spp. and substituent 16S rRNA sequencing to determine the prevailing strains in the two locations. Results: Sixty-two Pseudomonas spp. were isolated from the two locations (27 isolates from CHB and 35 isolates from the UBTH). Surgical wound infections screened with regularly used antibiotics revealed that 17 (62.9%) isolates from CHB and 20 (57.1%) isolates from UBTH were multiple drug resistant Pseudomonas spp. PCR identification using Pseudomonas spp. specific primer showed that 16 (94.1%) isolates from CHB and 18 (90%) isolates from UBTH were confirmed. 16S DNA sequencing revealed that P. aeruginosa strain H25883 was dominant in both locations. Conclusion: High antibiotic resistance among P. aeruginosa isolates was established in our study. PCR technique revealed a more reliable method of bacterial identification. H25883 strain of P. aeruginosa is the prevalent strain in both locations and it should be given attention in nosocomial surgical wound infections.

scholarly journals Unusual Multiple Brain Abscesses Caused by Aeromonas hydrophila in a Preterm Neonate: Case Report

2018 ◽  
Vol 08 (01) ◽  
pp. e55-e57
Author(s):  
Elham Bukhari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Brain Abscess ◽  
Aeromonas Hydrophila ◽  
Preterm Neonate ◽  
Preterm Newborn ◽  
Neonatal Brain ◽  
Gram Negative ◽  
Facultative Anaerobic ◽  
Brain Abscesses ◽  
Citrobacter Diversus

AbstractNeonatal brain abscess is rare. The most common etiologic microorganisms that are responsible for this condition are Citrobacter diversus, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other Enterobacteriaceae. Aeromonas hydrophila is a halophilic, gram-negative, facultative anaerobic bacillus.It has been reported to cause intestinal and extraintestinal infections and has rarely been reported to cause meningitis at neonatal age. A. hydrophila has not been reported previously to cause brain abscess in neonates. In this report, we describe a case of severe multiple brain abscesses and ventriculitis as complications in meningitis caused by A. hydrophila in a preterm newborn.

The Antimicrobial Activity of Extracts of the Lichen Cetraria aculeata and Its Protolichesterinic Acid Constituent

2003 ◽  
Vol 58 (11-12) ◽  
pp. 850-854 ◽  
Author(s):  
Ayşen Özdemir Türk ◽  
Meral Yılmaz ◽  
Merih Kıvanç ◽  
Hayrettin Türk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Listeria Monocytogenes ◽  
Bacillus Cereus ◽  
Aeromonas Hydrophila ◽  
Proteus Vulgaris ◽  
Ethanol Extracts

Abstract In this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen Cetraria aculeata has been investigated. The extracts were tested against twelve bacteria and eight fungi and found active against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, Proteus vulgaris, Streptococcus faecalis, Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes. No antimicrobial activity against the fungi was detected. It was determined that only one substance in the extracts has antimicrobial activity and it was characterized as protolichesterinic acid. The MICs of the extracts and protolichesterinic acid were also determined.

scholarly journals Distribution de Pseudomonas aeruginosa et Aeromonas hydrophila dans les eaux de la nappe phréatique superficielle en zone équatoriale au Cameroun et relations avec quelques paramètres chimiques du milieu.

2005 ◽  
Vol 14 (1) ◽  
pp. 35-53 ◽  
Author(s):  
M. Nola ◽  
T. Njine ◽  
V. F. Sikati ◽  
E. Djuikom
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Aeromonas Hydrophila

Une étude microbiologique et chimique a été menée pendant un an sur les eaux de sources et de puits de Yaoundé (Cameroun). Les analyses microbiologiques ont été faites suivant la technique des membranes filtrantes, et les analyses chimiques, suivant les techniques analytiques usuelles. Les abondances maximales mensuelles de Pseudomonas aeruginosa et de Aeromonas hydrophila varient respectivement de 1 à 22x103 UFC.100 ml-1 d'eau, et de 1 à 7,8x103 UFC.100 ml-1. Ces abondances bactériennes subissent d'amples fluctuations spatio-temporelles. Les eaux de sources et de puits analysées sont faiblement bicarbonatées, douces et présentent une minéralisation faible à moyenne. Le pH varie de 3 à 5 et les concentrations en CO2 dissous, de 300 à 532 mg.l-1. Un bon nombre des caractéristiques chimiques de ces eaux est relativement stable au cours du temps, en dépit des fluctuations spatiales apparentes. Le degré de corrélation entre les paramètres chimiques et la dynamique d'abondance des bactéries isolées, est hétérogène. Cela est nettement plus marqué dans les eaux de puits, en raison des conséquences de l'exploitation humaine de ces eaux, à l'origine d'apports allochtones divers.

scholarly journals Detection of prevalence of metallo-beta lactamases in clinical isolates of imipenem resistant Pseudomonas aeruginosa from neonatal septicaemia cases in a tertiary hospital in Odisha, India

2017 ◽  
Vol 5 (1) ◽  
pp. 61 ◽  
Author(s):  
Surya Narayan Mishra ◽  
Seba Ranjan Biswal ◽  
Basanta Kumar Behera ◽  
Dipti Pattnaik
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Isolates ◽  
Beta Lactamase ◽  
Strict Adherence ◽  
Neonatal Septicemia ◽  
Gram Negative ◽  
Beta Lactamases ◽  
Pseudomonas Spp ◽  
Neonatal Septicaemia ◽  
Synergy Test

Background: Pseudomonas aeruginosa is a clinically troublesome gram-negative pathogen that causes both opportunistic infections and nosocomial outbreaks. Metallo beta lactamase have recently emerged as a worrisome resistance mechanism. Carbapenems had been the drug of choice for the infections caused by most penicillin- or cephalosporin-resistant gram-negative bacteria due to its broad-spectrum activity and stability to hydrolysis by most beta-lactamases. This does not hold good anymore due to rapid uprise of MBL producing strains. The current research covered 163 hospitalized cases of neonatal septicaemia from which Pseudomonas aeruginosa is isolated in the Paediatric Department of KIMS, Bhubaneswar. The study aimed at detecting the prevalence of metallo-beta lactamases in clinical isolates of imipenem resistant Pseudomonas aeruginosa from neonatal septicemia cases and to establish the antibiogram of Imipenem-resistant P. aeruginosa these cases. Methods: Clinical samples obtained from suspected cases of neonatal septicemia were first cultured by conventional methods and then identification was done by VITEK-2 instrument. Metallo beta lactamase (MBL) production was done by combined disc synergy test (CDST) using imipenem and EDTA (CDST-IPM) and double disc synergy test (DDST) using IPM and EDTA (DDST-IPM). Results: Among 1510 processed clinical specimens from cases of neonatal septicaemia; 637 (42.18%) showed positive growth of various clinically significant pathogens. Out of them in 163 (25.58%) cases Pseudomonas spp. was isolated. Of these, a total of 95 (58.28%) Pseudomonas spp. was found resistant to imipenem. All imipenem-resistant Pseudomonas isolates were positive for MBL by CDST imipenem-EDTA (CDST-IPM) method, whereas 89 (93.68%) were positive by DDST-IPM method, respectively. Pseudomonas aeruginosa was mostly isolated from endotracheal tube aspirate (57.89%) followed by pus (56.41%). Out of the 95 cases of MBL-producing Pseudomonas; 46 (48.42%) isolates showed the maximum susceptibility to piperacillin-tazobactam combination. All MBL-producing Pseudomonas isolates were resistant to ceftriaxone.Conclusions: MBL-producing Pseudomonas is found to be highly prevalent in our hospital, which is one of the major causes of multidrug resistance and need regular surveillance and strict adherence to a robust antibiotic policy.

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