scholarly journals Chlorophyll-associated genes and SSR markers linked to genetic variations in heat tolerance of perennial ryegrass

2020 ◽  
Author(s):  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
Zhipeng Zhang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Genetic Variations

scholarly journals Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
Zhipeng Zhang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Heat Tolerant ◽  
Cool Season Grass

Abstract Background Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.). Results Plants of 98 accessions were subjected to heat stress (35/30 °C, day/night) or optimal growth temperature (25/20 °C) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (− 0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2 > 0.05, p < 0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL. Conclusion The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

scholarly journals Natural Variation of Physiological Traits, Molecular Markers, and Chlorophyll Catabolic Genes Associated With Heat Tolerance in Perennial Ryegrass Accessions

2020 ◽  
Author(s):  
Bin Xu ◽  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Principal Component ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Cool Season Grass

Abstract Background: Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.). Results: Plants of 98 accessions were subjected to heat stress (35/30 ℃, day/night) or optimal growth temperature (25/20 ℃) for 24 d in growth chambers. Overall heattolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (−0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were closely related to the heat tolerance of ryegrass accessions. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2>0.05, p<0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL.Conclusion:The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

scholarly journals Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions

2020 ◽  
Author(s):  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
Zhipeng Zhang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Heat Tolerant ◽  
Cool Season Grass

Abstract Background: Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.).Results: Plants of 98 accessions were subjected to heat stress (35/30 ℃, day/night) or optimal growth temperature (25/20 ℃) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (−0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2>0.05, p <0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL.Conclusion: The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

scholarly journals Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions

2020 ◽  
Author(s):  
Bin Xu ◽  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Heat Tolerant ◽  
Cool Season Grass

Abstract Background: Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.). Results: Plants of 98 accessions were subjected to heat stress (35/30 ℃, day/night) or optimal growth temperature (25/20 ℃) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (−0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2>0.05, p <0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL. Conclusion: The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

Genetic Diversity in Seven Perennial Ryegrass ( Lolium perenne L.) Cultivars Based on SSR Markers

Crop Science ◽  
2001 ◽  
Vol 41 (5) ◽  
pp. 1565-1572 ◽  
Author(s):  
Christine Kubik ◽  
Mark Sawkins ◽  
William A. Meyer ◽  
Brandon S. Gaut
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Lolium Perenne L

Identification of genetic variations of cultivated and weedy types of Perilla species in Korea and Japan using morphological and SSR markers

2013 ◽  
Vol 35 (5) ◽  
pp. 649-659 ◽  
Author(s):  
Kyu Jin Sa ◽  
Seung Hun Choi ◽  
Mariko Ueno ◽  
Kyong-Cheul Park ◽  
Young Jin Park ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Variations ◽  
Cultivated And Weedy Types

Bulked Segregant Analysis to Detect QTL Related to Heat Tolerance in Rice (Oryza sativa L.) Using SSR Markers

2009 ◽  
Vol 8 (4) ◽  
pp. 482-487 ◽  
Author(s):  
Gui-lian ZHANG ◽  
Li-yun CHEN ◽  
Guo-ying XIAO ◽  
Ying-hui XIAO ◽  
Xin-bo CHEN ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis

Use of modelling to identify perennial ryegrass plant traits for future warmer and drier climates

2014 ◽  
Vol 65 (8) ◽  
pp. 758 ◽  
Author(s):  
B. R. Cullen ◽  
R. P. Rawnsley ◽  
R. J. Eckard ◽  
K. M. Christie ◽  
M. J. Bell
Keyword(s):  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Plant Traits ◽  
Climate Projections ◽  
Environment Interaction ◽  
Root Depth ◽  
Pasture Production ◽  
Local Climate ◽  
High Rainfall ◽  
Eastern Australia

Potential exists to select pasture species better adapted to anticipated warmer temperatures and lower rainfall, associated with increasing atmospheric carbon dioxide (CO2) and other greenhouse gas concentrations, to maximise pasture yields and persistence. This study assessed the effect of increasing three plant traits in perennial ryegrass (Lolium perenne L.) to adapt to future climates: root depth; heat tolerance, defined as the ability of plant to grow at high temperatures; and responsiveness to elevated CO2 concentrations. Pasture production was simulated using the Sustainable Grazing Systems Pasture model at three sites with temperate climates in south-eastern Australia: Hamilton, Victoria (medium rainfall); Ellinbank, Victoria (high rainfall); and Elliott, Tasmania (high rainfall). Two future climate scenarios were created at each site by scaling the historical climate (1971–2010) by +1°C with –10% rain (435 ppm CO2) and +2°C with –20% rain (535 ppm CO2). A genotype × environment interaction suggested that the plants traits most effective at increasing pasture yield differed depending on the local climate. Increased root depth was the most effective change in a single trait that increased pasture harvested at Elliott, increased heat tolerance was most effective at Ellinbank, whereas increasing all three individual traits was similarly effective at Hamilton. At each site, the most effective traits increased pasture growth during the period between late spring and mid-summer compared with the current cultivar. When all three traits were increased at the same time, the pasture production advantage was greater than the additive effects of changing single traits at Hamilton and Ellinbank. Further consideration of the feasibility of selecting multiple traits and the effects of a broader range of climate projections is required. Nonetheless, results of this study provide guidance to plant breeders for selection of traits adapted to future climates.

Genetic variations of Chinese melon landraces investigated with EST-SSR markers

2011 ◽  
Vol 52 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Qiusheng Kong ◽  
Changping Xiang ◽  
Jin Yang ◽  
Zhongwei Yu
Keyword(s):  
Ssr Markers ◽  
Genetic Variations
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