scholarly journals Exogenous Fatty Acid Hydroperoxide Perception as Elicitor Is Related to Modulation of Plant Plasma Membrane Structure

2021 ◽  
Author(s):  
Estelle Deboever ◽  
Géraldine van Aubel ◽  
Valeria Rondelli ◽  
Alexandros Koutsioumpas ◽  
Marion Mathelie-Guinlet ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Mechanism Of Action ◽  
Membrane Lipids ◽  
Plant Protection ◽  
Lipid Fraction ◽  
Plant Defence ◽  
In Planta ◽  
Exogenous Fatty Acid ◽  
Plant Plasma Membrane

Fatty acid hydroperoxides (HPOs) are amphiphilic molecules naturally produced by plants in stressed conditions and involved in plant immunity as signalling molecules. Although some studies report their potential use as exogenous biocontrol agents for plant protection, evaluation of their efficiency in planta is lacking and no information is available about their mechanism of action. In this work, the potential of two HPO forms, 13-HPOD and 13-HPOT, as plant defence elicitors and the underlying mechanism of action are investigated. Both HPOs trigger Arabidopsis innate immunity. They increase plant resistance to the pathogenic fungi Botrytis cinerea and activate early immunity-related defence responses, like ROS production. As our previous study has suggested that HPOs are able to interact with the plant plasma membrane (PPM) lipid fraction, we have further investigated the effects of HPOs on biomimetic PPM structure using complementary biophysics tools. Results show that HPO insertion into PPM impacts its global structure without solubilizing it. 13-HPOT, with an additional double bond compared to 13-HPOD, exerts a higher effect by fluidifying and reducing the thickness of the bilayer. Correlation between biological assays and biophysical analysis suggests that lipid amphiphilic elicitors that directly act on membrane lipids might trigger early plant defence events.

scholarly journals Is Exogenous Fatty Acid Hydroperoxide Perception as Elicitor Related to Modulation of Plant Plasma Membrane Structure ?

2021 ◽  
Author(s):  
Estelle Deboever ◽  
Géraldine van Aubel ◽  
Valeria Rondelli ◽  
Alexandros Koutsioumpas ◽  
Marion Mathelie-Guinlet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Mechanism Of Action ◽  
Plasma Membranes ◽  
Membrane Lipids ◽  
Plant Protection ◽  
Plant Defence ◽  
Underlying Mechanism ◽  
Dienoic Acid ◽  
In Planta ◽  
Exogenous Fatty Acid

Oxylipins are lipid-derived molecules that are ubiquitous in eukaryotes and whose functions in plant physiology have been widely reported. They appear to play a major role in plant immunity by orchestrating reactive oxygen species (ROS) and hormone-dependent signalling pathways. The present work focuses on the specific case of fatty acid hydroperoxides (HPOs). Although some studies report their potential use as exogenous biocontrol agents for plant protection, evaluation of their efficiency in planta is lacking and no information is available about their mechanism of action. In this work, the potential of 13(S)-hydroperoxyoctadeca-(9Z,11E)-dienoic acid (13-HPOD) and 13(S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoic acid (13-HPOT), as plant defence elicitors and the underlying mechanism of action are investigated. Arabidopsis thaliana leaf resistance to Botrytis cinerea was observed after root application with HPOs. They also activate early immunity-related defence responses, like ROS. As previous studies have demonstrated their ability to interact with plant plasma membranes (PPM), we have further investigated the effects of HPOs on biomimetic PPM structure using complementary biophysics tools. Results show that HPO insertion into PPM impacts its global structure without solubilizing it. Relationship between biological assays and biophysical analysis suggests that lipid amphiphilic elicitors that directly act on membrane lipids might trigger early plant defence events

Modulation of Plant Plasma Membrane Structure by Exogenous Fatty Acid Hydroperoxide is a Potential Perception Mechanism for their Eliciting Activity

2021 ◽  
Author(s):  
Deboever Estelle ◽  
Van Aubel Géraldine ◽  
Rondelli Valeria ◽  
Koutsioumpas Alexandros ◽  
Mathelie‐Guinlet Marion ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Membrane Structure ◽  
Fatty Acid Hydroperoxide ◽  
Exogenous Fatty Acid ◽  
Acid Hydroperoxide ◽  
Plant Plasma Membrane

scholarly journals Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

2015 ◽  
Vol 170 (1) ◽  
pp. 367-384 ◽  
Author(s):  
Jean-Luc Cacas ◽  
Corinne Buré ◽  
Kevin Grosjean ◽  
Patricia Gerbeau-Pissot ◽  
Jeannine Lherminier ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Lipids ◽  
Plant Plasma Membrane

scholarly journals Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

2017 ◽  
Vol 83 (22) ◽  
Author(s):  
Anna R. Moravec ◽  
Andrew W. Siv ◽  
Chelsea R. Hobby ◽  
Emily N. Lindsay ◽  
Layla V. Norbash ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Membrane Permeability ◽  
Stress Responses ◽  
Biofilm Formation ◽  
Membrane Lipids ◽  
Regulation Of Gene Expression ◽  
Exogenous Fatty Acid ◽  
Content Type

ABSTRACT The pathogenic Vibrio species (V. cholerae, V. parahaemolyticus, and V. vulnificus) represent a constant threat to human health, causing foodborne and skin wound infections as a result of ingestion of or exposure to contaminated water and seafood. Recent studies have highlighted Vibrio's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. The possession and conservation of such machinery provokes consideration of fatty acids as important factors in the pathogenic lifestyle of Vibrio species. The findings here link exogenous fatty acid exposure to changes in bacterial membrane phospholipid structure, permeability, phenotypes associated with virulence, and consequent stress responses that may impact survival and persistence of pathogenic Vibrio species. Polyunsaturated fatty acids (PUFAs) (ranging in carbon length and unsaturation) supplied in growth medium were assimilated into bacterial phospholipids, as determined by thin-layer chromatography and liquid chromatography-mass spectrometry. The incorporation of fatty acids variably affected membrane permeability, as judged by uptake of the hydrophobic compound crystal violet. For each species, certain fatty acids were identified as affecting resistance to antimicrobial peptide treatment. Significant fluctuations were observed with regard to both motility and biofilm formation following growth in the presence of individual PUFAs. Our results illustrate the important and complex roles of exogenous fatty acids in the membrane physiology and virulence of a bacterial genus that inhabits aquatic and host environments containing an abundance of diverse fatty acids. IMPORTANCE Bacterial responses to fatty acids include, but are not limited to, degradation for metabolic gain, modification of membrane lipids, alteration of protein function, and regulation of gene expression. Vibrio species exhibit significant diversity with regard to the machinery known to participate in the uptake and incorporation of fatty acids into their membranes. Both aquatic and host niches occupied by Vibrio are rife with various free fatty acids and fatty acid-containing lipids. The roles of fatty acids in the environmental survival and pathogenesis of bacteria have begun to emerge and are expected to expand significantly. The current study demonstrates the responsiveness of V. cholerae, V. parahaemolyticus, and V. vulnificus to exogenous PUFAs. In addition to phospholipid remodeling, PUFA assimilation impacts membrane permeability, motility, biofilm formation, and resistance to polymyxin B.

scholarly journals Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies

2019 ◽  
Vol 20 (5) ◽  
pp. 1009 ◽  
Author(s):  
Noadya Monnier ◽  
Aurélien Furlan ◽  
Sébastien Buchoux ◽  
Magali Deleu ◽  
Manuel Dauchez ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Bilayers ◽  
Plasma Membranes ◽  
Plant Protection ◽  
Md Simulations ◽  
Lipid Dynamics ◽  
Defense Induction ◽  
Membrane Models ◽  
Plant Plasma Membrane ◽  
Membrane Destabilization

Rhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the molecular scale. Representative models were constructed and the interaction with RLs was studied by Fourier transform infrared (FTIR) and deuterium nuclear magnetic resonance (2H NMR) spectroscopic measurements. Molecular dynamic (MD) simulations were performed to investigate RL insertion in lipid bilayers. Our results showed that the RLs fit into the membrane models and were located near the lipid phosphate group of the phospholipid bilayers, nearby phospholipid glycerol backbones. The results obtained with plant plasma membrane models suggest that the insertion of RLs inside the lipid bilayer did not significantly affect lipid dynamics. Oppositely, a clear fluidity increase of fungi membrane models was observed. This effect was related to the presence and the specific structure of ergosterol. The nature of the phytosterols could also influence the RL effect on plant plasma membrane destabilization. Subtle changes in lipid dynamics could then be linked with plant defense induction and the more drastic effects associated with fungal membrane destabilization.

scholarly journals The bacterial lipopeptide surfactin targets the lipid fraction of the plant plasma membrane to trigger immune-related defence responses

2011 ◽  
Vol 13 (11) ◽  
pp. 1824-1837 ◽  
Author(s):  
Guillaume Henry ◽  
Magali Deleu ◽  
Emmanuel Jourdan ◽  
Philippe Thonart ◽  
Marc Ongena
Keyword(s):  
Plasma Membrane ◽  
Lipid Fraction ◽  
Defence Responses ◽  
Plant Plasma Membrane

In vivo esterification of a synthetic 125I-labeled fatty acid into cardiac glycerolipids

1983 ◽  
Vol 245 (4) ◽  
pp. H693-H697
Author(s):  
K. R. Chien ◽  
A. Han ◽  
J. White ◽  
P. Kulkarni
Keyword(s):  
Fatty Acid ◽  
Membrane Lipids ◽  
Lipid Fraction ◽  
Synthetic Fatty Acid ◽  
Water Soluble ◽  
Myocardial Imaging ◽  
Sprague Dawley ◽  
Membrane Phospholipids ◽  
Triglyceride Fraction

Recent studies have demonstrated that fatty acids can be successfully utilized as myocardial imaging agents. 125I-paraphenylpentadecanoic acid (IPPA), a synthetic fatty acid, accumulates within the myocardium and can be visualized by conventional gamma scintigraphy. To determine if IPPA was incorporated into cardiac lipids in a pattern similar to palmitate, IPPA was purified by liquid chromatography, bound to fat-free albumin, and administered by intravenous injection to male Sprague-Dawley rats. After 2.5, 5, 10, and 30 min, the hearts were excised and the lipids were extracted in chloroform-methanol. The uptake of IPPA into the myocardium reached a maximal value after 2.5 min, and 95% of the 125I was found in the cardiac lipid fraction after chromatographic separation. Over 65% of the IPPA was found in cardiac triglycerides, whereas approximately 10% was present in membrane phospholipids (predominantly phosphatidylcholine and phosphatidylethanolamine). This pattern of IPPA incorporation is similar to that reported for intravenously administered [3H]palmitate. The rate of turnover of IPPA present in the triglyceride fraction was threefold faster than the rate of the IPPA which was incorporated into membrane lipids. At all time periods examined, the methanol-water soluble end products of IPPA oxidation did not account for more than 5% of the total IPPA present within the myocardium. The present study indicates that IPPA is incorporated primarily into triglycerides and other cardiac lipids in a pattern similar to palmitate.

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