scholarly journals A novel antifungal peptide purified from Bacillus subtilis strain EDR4

2016 ◽  
Vol 68 (2) ◽  
pp. 319-324
Author(s):  
Jingyuan Ji ◽  
Jie Yang ◽  
Lili Huang ◽  
Zhensheng Kang
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Biocontrol Agent ◽  
Pathogenic Fungi ◽  
Subtilis Strain ◽  
Antifungal Peptide ◽  
Inhibitory Effects ◽  
Canker Disease ◽  
Ph Values ◽  
Potential Biocontrol Agent

Bacillus subtilis strain EDR4 is a potential biocontrol agent against Valsa mali (Vm), the fungus causing apple canker disease. In this study, we identified and characterized the antifungal peptide produced by B. subtilis EDR4. Peptides were isolated by 30% ammonium sulfate precipitation and purified by column chromatography. A 4-kDa peptide exhibiting antifungal activity was obtained and designated as P6. The amino acid sequence of P6 was determined by liquid chromatography combined with tandem mass spectrometry. P6 showed inhibitory effects against eight different pathogenic plant fungi, and was stable (i.e., retained its biological activity) at temperatures as high as 121?C for 20 min and at pH values ranging from 3 to 11. Microscopic examination of Vm hyphae treated with P6 showed protoplasm release, and in vitrospore germination was also inhibited. These results suggest that P6 is the active substance responsible for the antifungal activity of B. subtilisEDR4 against Vmand that P6 may be effective in the biocontrol of Vmas well as other plant pathogenic fungi.

scholarly journals Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro

2005 ◽  
Vol 160 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Bhaskar Chaurasia ◽  
Anita Pandey ◽  
Lok Man S. Palni ◽  
Pankaj Trivedi ◽  
Bhavesh Kumar ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Volatile Compounds ◽  
Pathogenic Fungi ◽  
Subtilis Strain ◽  
Structural Deformations

Characterization and sequence analysis of potential biofertilizer and biocontrol agent Bacillus subtilis strain SEM-9 from silkworm excrement

2019 ◽  
Vol 65 (1) ◽  
pp. 45-58 ◽  
Author(s):  
Qingrong Li ◽  
Sentai Liao ◽  
Huyu Zhi ◽  
Dongxu Xing ◽  
Yang Xiao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Gene Clusters ◽  
Antagonistic Effect ◽  
Subtilis Strain ◽  
Fusarium Spp ◽  
Antagonistic Microorganisms ◽  
Biochemical Analyses ◽  
Physiological And Biochemical

Fusarium wilt is a devastating soil-borne disease caused mainly by highly host-specific formae speciales of Fusarium oxysporum. Antagonistic microorganisms play a very important role in Fusarium wilt control, and the isolation of potential biocontrol strains is becoming more and more important. We isolated a bacterial strain (SEM-9) from the high-temperature stage of silkworm excrement composting, which had a marked ability to solubilize phosphorus, promote the growth and increase the yield of the small Chinese cabbage, and which also exhibited considerable antagonistic effect towards Fusarium sambucinum and other fungi. The result of physiological and biochemical analyses, as well as genome sequencing, showed that SEM-9 was a strain of Bacillus subtilis. Through genome annotation and analysis, it was found that SEM-9 contained genes related to the regulation of biofilm formation, which may play an important role in colonization, and gene clusters encoding the biosynthesis of antimicrobials, such as surfactin, bacilysin, fengycin, and subtilosin-A. The production of such antifungal compounds may constitute the basis of the mode-of-action of SEM-9 against Fusarium spp. These data suggested that the SEM-9 strain has potential as both a biofertilizer and a biocontrol agent, with the potential to manage Fusarium wilt disease in crops.

scholarly journals Complete Genome Sequence of Bacillus subtilis J-5, a Potential Biocontrol Agent

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Zhenhua Jia ◽  
Weiwei Jin ◽  
Yali Huang ◽  
Shuishan Song
Keyword(s):  
Bacillus Subtilis ◽  
Biocontrol Agent ◽  
Rhizosphere Soil ◽  
Whole Genome ◽  
Antimicrobial Compounds ◽  
Content Type ◽  
Regulatory Systems ◽  
Potential Biocontrol Agent ◽  
Genes Encoding ◽  
Shed Light

ABSTRACT Bacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea. To shed light on the molecular mechanism underlying the biological control on phytopathogens, the whole genome of this strain was sequenced. Genes encoding antimicrobial compounds and the regulatory systems were identified in the genome.

scholarly journals Chitinolytic Bacillus subtilis Ege-B-1.19 as a biocontrol agent against mycotoxigenic and phytopathogenic fungi

2018 ◽  
Vol 44 (3) ◽  
pp. 323-331
Author(s):  
Ozlem Oztopuz ◽  
Nermin Sarigul ◽  
Fakhra Liaqat ◽  
Ro-Dong Park ◽  
Rengin Eltem
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Biocontrol Agent ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Aspergillus Ochraceus ◽  
Lytic Enzyme ◽  
Rrna Gene ◽  
Lytic Enzymes ◽  
16S Rrna Gene Analysis

Abstract Background Biological control of pathogenic fungi is a possible alternate to the chemical control, which is harmful to humans and environment. Soil-borne Bacillus strains can be potential biocontrol agents and a source of lytic enzymes. Aim This study aimed to examine biocontrol potential and lytic enzyme activities of a soil isolate Bacillus subtilis Ege-B-1.19. Materials and methods Strain was identified by biochemical and 16S rRNA gene analysis and its biocontrol activity was investigated against Aspergillus niger EGE-K-213, Aspergillus foetidus EGE-K-211, Aspergillus ochraceus EGE-K-217, Fusarium solani KCTC6328, Rhizoctonia solani KACC40111 and Colletotrichum gloeosporioides KACC40689. Chitinase, chitosanase, N-acetyl-β-hexosaminidase and protease activities of B. subtilis Ege-B-1.19 were also determined. Chitosanase was purified using Sephadex G-150 column and its molecular weight was determined by SDS-PAGE. Chitooligosaccharides production using chitosanase was carried out and analysed by TLC and HPLC. Results Results depicted that B. subtilis Ege-B-1.19 has shown inhibitory effects against all the test fungi. Chitinase, chitosanase, N-acetyl-β-hexosaminidase and protease activities were determined as 2.7 U mL−1, 7.2 U mL−1, 6.2 U mL−1 and 12.2 U mL−1, respectively. Molecular weight of purified chitosanase was 44 kDa. Chitosanase hydrolysed chitosan to glucosamine (GlcN), dimers (GlcN)2 and trimers (GlcN)3. Conclusion Bacillus subtilis Ege-B-1.19 can be anticipated as useful biocontrol agent and its chitosanase can be utilized for enzymatic synthesis of chitooligosaccharides.

scholarly journals Isolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Xuechao Zhang ◽  
Xiaojun Guo ◽  
Cuihong Wu ◽  
Chengcui Li ◽  
Dongdong Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Pichia Pastoris ◽  
Fungal Infections ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Exchange Chromatography ◽  
Proteinase K ◽  
Antifungal Protein ◽  
Subtilis Strain

Abstract Background Wheat sheath blight, a soil borne fungal disease caused by Rhizoctonia cerealis, is considered as one of the most serious threats to wheat worldwide. Bacillus subtilis Z-14 was isolated from soil sampled from a wheat rhizosphere and was confirmed to have strong antifungal activity against R. cerealis. Results An antifungal protein, termed F2, was isolated from the culture supernatant of Z-14 strain using precipitation with ammonium sulfate, anion exchange chromatography, and reverse phase chromatography. Purified F2 had a molecular mass of approximately 8 kDa, as assessed using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Edman degradation was used to determine the amino acid sequence of the N-terminus, which was NH2ASGGTVGIYGANMRS. This sequence is identical to a hypothetical protein RBAM_004680 (YP_001420098.1) synthesized by B. amyloliquefaciens FZB42. The recombinant F2 protein (rF2) was heterologously expressed in the yeast host Pichia pastoris, purified using a Niaffinity column, and demonstrated significant antifungal activity against R. cerealis. The purified rF2 demonstrated broad spectrum antifungal activity against different varieties of fungi such as Fusarium oxysporum, Verticillium dahliae, Bipolaris papendorfii, and Fusarium proliferatum. rF2 was thermostable, retaining 91.5% of its activity when incubated for 30 min at 100 °C. Meanwhile, rF2 maintained its activity under treatment by proteinase K and trypsin and over a wide pH range from 5 to 10. Conclusions A novel antifungal protein, F2, was purified from biocontrol Bacillus subtilis Z-14 strain fermentation supernatant and heterologously expressed in Pichia pastoris to verify its antifungal activity against R. cerealis and the validity of the gene encoding F2. Considering its significant antifungal activity and stable characteristics, protein F2 presents an alternative compound to resist fungal infections caused by R. cerealis.

scholarly journals Formulation development of the biocontrol agent Bacillus subtilis strain CPA-8 by spray-drying

2012 ◽  
Vol 112 (5) ◽  
pp. 954-965 ◽  
Author(s):  
V. Yánez-Mendizábal ◽  
I. Viñas ◽  
J. Usall ◽  
R. Torres ◽  
C. Solsona ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Spray Drying ◽  
Biocontrol Agent ◽  
Subtilis Strain ◽  
Formulation Development

scholarly journals RhizosphericBacillus subtilisExhibits Biocontrol Effect againstRhizoctonia solaniin Pepper(Capsicum annuum)

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yuanyuan Huang ◽  
Zhansheng Wu ◽  
Yanhui He ◽  
Bang-Ce Ye ◽  
Chun Li
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Capsicum Annuum ◽  
Mycelial Growth ◽  
Biocontrol Agent ◽  
Growth Strain ◽  
Inhibition Rate ◽  
Pepper Plants

This study aimed at evaluating the ability of SL-44 to controlRhizoctonia solaniand promote pepper(Capsicum annuum)growth. Strain SL-44 was isolated from plant rhizosphere and the pot experiment results indicated that the dry and fresh weights of pepper in SL-44 andRhizoctonia solani(S-R) treatment were 45.5% and 54.2% higher than those inRhizoctonia solani(R) treatment and 18.2% and 31.8% higher than those in CK (control, noninoculation) treatment. The plant height in S-R treatment increased by 14.2% and 9.0% compared with those in the R and CK treatments, respectively. In vitro antagonism assay showed that SL-44 exhibited strong antifungal activity against the mycelial growth ofRhizoctonia solani, with an inhibition rate of 42.3%. The amount of phosphorus dissolved by SL-44 reached 60.58 mg·L−1in broth and 7.5 μg·mL−1IAA were secreted by SL-44. Strain SL-44 inhibited the growth ofR. solaniand improved biomass of pepper plants. Mass exchange and information transmission between the pepper plants and SL-44 mutually promoted their development.Bacillus subtilisSL-44 has a great potential as biocontrol agent againstRhizoctonia solanion pepper plants.

Identification and evaluation of a potential biocontrol agent, Bacillus subtilis, against Fusarium sp. in apple seedlings

2013 ◽  
Vol 64 (1) ◽  
pp. 377-383 ◽  
Author(s):  
Ruicheng Ju ◽  
Yuhua Zhao ◽  
Jinyu Li ◽  
Hongxia Jiang ◽  
Peng Liu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Biocontrol Agent ◽  
Potential Biocontrol Agent ◽  
Fusarium Sp
Sign in / Sign up

Export Citation Format

Share Document