Isolation and identification of a potential biocontrol agent Bacillus subtilis QM3 from Qinghai yak dung in China

2008 ◽  
Vol 24 (11) ◽  
pp. 2451-2458 ◽  
Author(s):  
Qing-Ping Hu ◽  
Jian-Guo Xu ◽  
Peng Song ◽  
Jin-Ning Song ◽  
Wu-Ling Chen
2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Zhenhua Jia ◽  
Weiwei Jin ◽  
Yali Huang ◽  
Shuishan Song

ABSTRACT Bacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea. To shed light on the molecular mechanism underlying the biological control on phytopathogens, the whole genome of this strain was sequenced. Genes encoding antimicrobial compounds and the regulatory systems were identified in the genome.


2013 ◽  
Vol 64 (1) ◽  
pp. 377-383 ◽  
Author(s):  
Ruicheng Ju ◽  
Yuhua Zhao ◽  
Jinyu Li ◽  
Hongxia Jiang ◽  
Peng Liu ◽  
...  

2016 ◽  
Vol 68 (2) ◽  
pp. 319-324
Author(s):  
Jingyuan Ji ◽  
Jie Yang ◽  
Lili Huang ◽  
Zhensheng Kang

Bacillus subtilis strain EDR4 is a potential biocontrol agent against Valsa mali (Vm), the fungus causing apple canker disease. In this study, we identified and characterized the antifungal peptide produced by B. subtilis EDR4. Peptides were isolated by 30% ammonium sulfate precipitation and purified by column chromatography. A 4-kDa peptide exhibiting antifungal activity was obtained and designated as P6. The amino acid sequence of P6 was determined by liquid chromatography combined with tandem mass spectrometry. P6 showed inhibitory effects against eight different pathogenic plant fungi, and was stable (i.e., retained its biological activity) at temperatures as high as 121?C for 20 min and at pH values ranging from 3 to 11. Microscopic examination of Vm hyphae treated with P6 showed protoplasm release, and in vitrospore germination was also inhibited. These results suggest that P6 is the active substance responsible for the antifungal activity of B. subtilisEDR4 against Vmand that P6 may be effective in the biocontrol of Vmas well as other plant pathogenic fungi.


Author(s):  
S Aswathi, C Ushamalini

Coriander is an important spice crop affected by wilt caused by Fusarium oxysporum f. sp. corianderii. Two trials were conducted to evaluate the efficacy of Bacillus subtilis for the management of wilt under glass house condition. The results of the trials indicated that, among the various treatments, seed treatment with talc formulation of Bacillus subtilis (10g/kg of seed) along with soil application (2.5 kg/ha) as basal and top dressing at 30 and 45 days after sowing was  effective in reducing the wilt incidence  (14.59%) compared to control (32.68% ).


2015 ◽  
Vol 68 ◽  
pp. 446-446
Author(s):  
D.J. Wilson ◽  
P.J. Gerard

Spiny snout mite (Neomolgus capillatus) is a potential biocontrol agent for clover flea (Sminthurus viridis) a white clover pest on dairy farms in warmer and wetter parts of New Zealand In the 1990s this mite was introduced from Brittany France into Tasmania for clover flea control Results during the release programme were highly promising and subsequent anecdotal farmer reports indicate widespread decreases in damage As N capillatus is a predatory mite and already known to attack nontarget organisms habitat specificity will determine whether it could be introduced into New Zealand without risk to native insects To assess this pastures on nine of the original Tasmanian release farms and adjacent nontarget habitats ranging from bush wetlands eucalypt stands to sand dune country were sampled in April 2014 Litter samples were collected heat extracted and mite species identified Neomolgus capillatus was found at effective densities in pastures that had good clover cover Where present it displaced Bdellodes spp mites that are ineffective against clover flea No N capillatus were found in the nontarget habitats all of which lacked clover and contained other predatory mites including Bdellodes spp Therefore the preference by N capillatus for lush pastures makes it an excellent prospect for introduction as a biocontrol agent into clover flea prone regions of New Zealand


Insects ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 664
Author(s):  
Ewa Puchalska ◽  
Stanisław Kamil Zagrodzki ◽  
Marcin Kozak ◽  
Brian G. Rector ◽  
Anna Mauer

Development, survival and reproduction of Ambyseius andersoni (Chant), a predatory mite widely distributed in Europe, were assessed on different food items. These included two key pests of ornamental coniferous plants, i.e., Oligonychus ununguis (Jacobi) and Pentamerismus taxi (Haller) and pollen of Pinus sylvestris L. The rationale behind these experiments was to provide a preliminary assessment of the potential of A. andersoni as a biocontrol agent of the above phytophagous arthropods and evaluate pine pollen as an alternative food source for the predator. Under laboratory conditions (23 ± 0.5 °C, 70 ± 10% RH and 16L:8D) A. andersoni was able to feed, develop and reproduce on all tested diets. The shortest development time (egg to female) was obtained when the predator fed on P. taxi (mean = 5.12 d) and the longest was on pine pollen (mean = 6.55 d). The rm value was significantly higher on both tested prey (0.166 on P. taxi and 0.160 on O. ununguis) than on pollen (0.139). Thus, we do not recommend pine pollen for mass rearing of A. andersoni; however, we conclude that pollen may provide sufficient sustenance for the predator population under field conditions when prey are absent. The potential of A. andersoni as a biocontrol agent of O. ununguis and P. taxi is discussed.


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