Response surface optimization of medium composition for alkaline protease production by Bacillus clausii

2008 ◽  
Vol 39 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Seyedeh Faranak Ghaemi Oskouie ◽  
Fatemeh Tabandeh ◽  
Bagher Yakhchali ◽  
Fereshteh Eftekhar
Keyword(s):  
Response Surface ◽  
Alkaline Protease ◽  
Medium Composition ◽  
Protease Production ◽  
Bacillus Clausii ◽  
Response Surface Optimization ◽  
Surface Optimization

scholarly journals Response surface optimization of medium composition for protease production by Bacillus subtilis using cassava waste

2011 ◽  
Vol 17 (2) ◽  
pp. 215-222 ◽  
Author(s):  
P. Rathakrishnan ◽  
P. Nagarajan ◽  
Rajesh Kannan
Keyword(s):  
Bacillus Subtilis ◽  
Response Surface ◽  
Waste Product ◽  
Maximum Growth ◽  
Positive Influence ◽  
Maximum Growth Rate ◽  
Medium Composition ◽  
Protease Production ◽  
Response Surface Optimization ◽  
Cassava Waste

Optimization of the growth condition for maximum growth rate and protease production was carried out using Bacillus subtilis. The optimization of protease production using agro industrial waste product such as cassava waste as substrate was performed with statistical methodology based on experimental designs. The screening of twelve nutrients for their influence on protease production was achieved using a Plackett-Burman design. MgSO4.7H2O, casein and glucose were selected based on their positive influence on protease production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are (% w/w): MgsO4.7H2O- 0.14, casein- 1.4 and glucose- 2.64. These conditions were validated experimentally which revealed an enhanced protease yield of 202.048 U/gds.

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