scholarly journals Molecular characterization of PCN populations from Serbia

Genetika ◽  
2012 ◽  
Vol 44 (1) ◽  
pp. 189-200
Author(s):  
Violeta Oro ◽  
Vesna Oro-Radovanovic
Keyword(s):  
Molecular Characterization ◽  
Globodera Pallida ◽  
Its2 Region ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Molecular Analyses ◽  
Morphological Studies ◽  
Similarities And Differences ◽  
Insight Into

The morphology of potato cyst nematodes (PCN) was until recently almost the only way to identify these quarantine organisms. In the last two decades, molecular analyses contributed to faster and more efficient identification of two Globodera species (Globodera pallida and G. rostochiensis) and allowed insight into the genetic structure of those parts that were practically inaccessible by morphological studies. Molecular characterization was performed in ITS1-5.8S-ITS2 region. The comparison was made with sequences of different foreign PCN populations via NCBI GenBank database. The results of molecular studies showed similarities and differences between local and foreign PCN populations in the part of genome that was studied.

scholarly journals A morphological and molecular characterization of vine mealybug populations (Hemiptera, Pseudococcidae) from Tunisia

2012 ◽  
Vol 44 (1) ◽  
pp. 5 ◽  
Author(s):  
Ramzi Mansour ◽  
Vincenzo Cavalieri ◽  
Gaetana Mazzeo ◽  
Kaouthar Grissa Lebdi ◽  
Agatino Russo
Keyword(s):  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Adult Female ◽  
Morphological Examination ◽  
Molecular Analyses ◽  
Planococcus Ficus ◽  
Morphological Studies ◽  
Vine Mealybug ◽  
Morphological And Molecular Characterization

Some vine mealybug, <em>Planococcus ficus </em>(Signoret) populations in Tunisian vineyards have been morphologically and genetically characterized. The morphological examination was based on the main distinctive characteristics of species of <em>Planococcus</em>, namely the number and distribution of the multilocular disc pores and tubular ducts on the adult female. This showed the existence of two different vine mealybug populations in Tunisia. Likewise, in the molecular analyses, two separate clades were revealed in the neighbour-joining phylogenetic tree, supporting the morphological studies and suggesting that there are two distinct populations of <em>P. ficus</em> on grapevine in Tunisia.

scholarly journals Taxonomy, Morphological and Molecular Identification of the Potato Cyst Nematodes, Globodera pallida and G. rostochiensis

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 184
Author(s):  
John Wainer ◽  
Quang Dinh
Keyword(s):  
Life Cycle ◽  
Molecular Identification ◽  
Soil Sampling ◽  
Globodera Pallida ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Primary Focus

The scope of this paper is limited to the taxonomy, detection, and reliable morphological and molecular identification of the potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis. It describes the nomenclature, hosts, life cycle, pathotypes, and symptoms of the two species. It also provides detailed instructions for soil sampling and extraction of cysts from soil. The primary focus of the paper is the presentation of accurate and effective methods to identify the two principal PCN species.

scholarly journals Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0241256
Author(s):  
Daniela Vallejo ◽  
Diego A. Rojas ◽  
John A. Martinez ◽  
Sergio Marchant ◽  
Claudia M. Holguin ◽  
...  
Keyword(s):  
Management Strategies ◽  
Nematode Species ◽  
Globodera Pallida ◽  
Rrna Gene ◽  
Potato Cyst Nematodes ◽  
28S Rrna ◽  
Cyst Nematodes ◽  
Nucleotide Substitutions ◽  
Internal Transcribed Spacer Region ◽  
The Status

Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in the potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples collected from 75 geographic localities in nine potato producing regions of Colombia (Cundinamarca, Boyacá, Antioquia, Nariño, Santander, Norte de Santander, Tolima, Caldas and Cauca) were assayed for the presence of potato cyst nematodes. Fifty-seven percent of samples tested positive for PCN. Based on phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rRNA gene and D2-D3 expansion segments of the 28S rRNA gene, all populations but one were identified as Globodera pallida. Sequences of G. pallida from Colombia formed a monophyletic group closely related to Peruvian populations, with the lowest average number of nucleotide substitutions per site (Dxy = 0.002) and net nucleotide substitutions per site (Da = 0.001), when compared to G. pallida populations from Europe, South and North America. A single sample formed a well-supported subclade along with G. rostochiensis and G. tabacum from Japan, USA and Argentina. To our knowledge this is the first comprehensive survey of Globodera populations from Colombia that includes genetic data. Our findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globodera species, and lead to the development of accurate management strategies for the potato cyst nematodes.

scholarly journals Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 70-74 ◽  
Author(s):  
P. Sedlák ◽  
M. Melounová ◽  
S. Skupinová ◽  
P. Vejl ◽  
J. Domkářová
Keyword(s):  
Animal Species ◽  
Molecular Genetic ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
Genetic Identification ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Genetic Dissimilarity ◽  
Solanaceous Plants ◽  
Molecular Genetic Identification

Potato cyst nematodes (PCN) are the big problem in worldwide planting of potatoes and another Solanaceous plants. Identification of individual pathotypes according to international scheme is very demanding but a very important part of the phytosanitary process to control these pests. Molecular genetic identification of different plant and animal species or individuals is a very interesting way at the present time and let&rsquo;s hope that it will be important in future. This report presents results of the RAPD study of nine different real PCN populations. There were five Globodera rostochiensis populations and four G. pallida populations. Pathotypes Ro2, Ro2/3, Ro4, Ro5, Pa2 and Pa3 were from European populations; population Ro1 and X were of Czech provenance. Genetics variable of these populations was described by a set of six decameric primers (OPA 07, OPG 03, OPG 05, OPG 08, OPG 10 and OPG 13). Genetic dissimilarity was by Gel Manager for Windows evaluated. Detectable differences behind all populations were found and the dendrogram was compiled. The unknown population X was sorted into group of Globodera pallida species subgroup of Pa2 consequently.

scholarly journals Variability of D2/D3 segment sequences of several populations and pathotypes of potato cyst nematodes (Globodera rostochiensis, Globodera pallida)

2010 ◽  
Vol 46 (No. 4) ◽  
pp. 171-180 ◽  
Author(s):  
O. Douda ◽  
M. Zouhar ◽  
E. Nováková ◽  
J. Mazáková ◽  
P. Ryšánek
Keyword(s):  
Dna Analysis ◽  
Pcr Amplification ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Routine Diagnostics ◽  
Pair Comparisons ◽  
Single Fragment ◽  
Direct Management

Potato cyst nematodes (Globodera rostochiensis, Globodera pallida) remain a key pest in the main potato growing regions of the Czech Republic. Due to difficult direct management and presence of diverse pathotypes attacking different potato cultivars the rapid and reliable diagnostics is of crucial importance. Currently, efforts are aimed at a description of different pathotypes based on DNA analysis. The main objective of this study was to evaluate the homogeneity of sequences of D2/D3 segments of the 28S rDNA gene obtained from 3 populations of G. rostochiensis and 5 populations of G. pallida and estimate their value for diagnostic purposes. PCR amplification yielded a single fragment of the length of 700 bp approximately in all populations. The alignment score of the vast majority of all pair comparisons of G. rostochiensis and G. pallida populations varied from 98 to 99. In total 14 point deletions and 3 substitutions were observed. The variability of D2/D3 segments of potato cyst nematodes is rather low and this DNA region can be used for diagnostics on a species level because more differences were found after comparing with G. tabacum and G. millefolii sequences obtained from Gene Bank; however the applicability of D2/D3 sequences to routine diagnostics of potato cyst nematodes could be complicated by its similarity to corresponding sequences of the nematode G. artemisiae.

Molecular Characterization of the Actin Gene from Cyst Nematodes in Comparison with Those from Other Nematodes

10.1654/4138 ◽  
2005 ◽  
Vol 72 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Elena S. Kovaleva ◽  
Sergei A. Subbotin ◽  
Edward P. Masler ◽  
David J. Chitwood
Keyword(s):  
Molecular Characterization ◽  
Actin Gene ◽  
Cyst Nematodes

Molecular characterization of Plum pox virus Rec isolates from Russia suggests a new insight into evolution of the strain

Virus Genes ◽  
2018 ◽  
Vol 54 (2) ◽  
pp. 328-332 ◽  
Author(s):  
Sergei Chirkov ◽  
Peter Ivanov ◽  
Anna Sheveleva ◽  
Anna Kudryavtseva ◽  
Irina Mitrofanova
Keyword(s):  
Molecular Characterization ◽  
Plum Pox Virus ◽  
Insight Into
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