scholarly journals Association between field and in vitro reaction to alternaria leaf blight in sunflower genotypes

Helia ◽  
2003 ◽  
Vol 26 (38) ◽  
pp. 109-114 ◽  
Author(s):  
S.M. Shaik ◽  
R.L. Ravikumar
2020 ◽  
Vol 56 (No. 3) ◽  
pp. 191-196
Author(s):  
Kahkashan Perveen ◽  
Najat A. Bokhari

The essential oil obtained by the hydro-distillation of the leaves of Mentha arvensis Linnaeus was evaluated for its antifungal activity against the causal agent of the Alternaria blight of tomatoes, i.e., Alternaria alternata (Fries) Keissler. The antifungal activity of the mentha essential oil was assessed both in vitro and in vivo. The chemical composition of the mentha oil was also identified by GCMS analysis. The in vitro test revealed that the maximum inhibition in the mycelial growth (93.6%) and conidia germination (90.6%) was at the highest concentration (40 µL/mL), furthermore, it was found that the inhibition of the mycelial growth and conidia germination was dose dependent. The in vivo test proved that the application of the mentha essential oil (40 µL/mL) significantly increased the plant height (84.6%), fresh weight (81.5%) and dry weight (80.0%) when compared to the untreated tomato plants. The disease incidence was 3.5 in the untreated plants, while it was 0.93 for the mentha essential oil treated plants and was 0.08 in the carbendazim treated plants. The GC-MS analysis of the mentha essential oil identified 18 compounds in total, among which the percentage of menthol was the highest (69.2%). The mentha essential oil was successful in managing the Alternaria leaf blight in the tomato plants. Therefore, it can be explored further for the development of a natural fungicide.


2020 ◽  
Vol 9 (3) ◽  
pp. 173-178
Author(s):  
Hafiz M. Saqib ◽  
Sobia Chohan ◽  
Muhammad Abid

Sunflower is an important oil seed crop of Pakistan, comprising 20% proteins and 38-45% oil contents. Alternaria leaf blight (ALB) caused by Alternaria alternata, is one of the devastating diseases of sunflower. Six different fungicides viz., difenoconazole, hexaconazole, azoxystrobin, dimethomorph + mancozeb, myclobutanil and Sulphur were tested at different concentrations (10, 20, 30 ppm) in laboratory and in greenhouse. Fungicides performed best in the laboratory were also investigated in greenhouse against Alternaria leaf blight of sunflower. In in vitro study, Hexaconazole showed 100 % growth inhibition of A. alternata at 30 ppm followed by 70% at 20 ppm and 62% at 10 ppm. Difenoconazole proved as the 2nd best fungicide with 77% fungal inhibition at 30 ppm, 75.8% at 20 ppm and 71% at 10 ppm. Azoxystrobin was the least effective fungicide with 24%, 28%, 34% fungal inhibition at 10, 20 and 30 ppm, respectively. Twelve cultivars of sunflower were screened against blight disease in pot experiment to check the fungicides at different level of susceptibility in greenhouse. Screening result showed that FH 702 was the  highest susceptible variety with mean value 7.6. Greenhouse study of disease inhibition effect of fungicides also showed that hexaconazole fungicide produced the best results against A. alternata with 42% disease reduction in FH 702 cultivar and 25 % in FH 696 cultivar as compared to control (83%). The results showed that no fungicide provided full disease inhibition, so, further investigation is needed to find the new chemistry against blight disease of sunflower crop


2021 ◽  
Vol 18 ◽  
Author(s):  
Jyoti Gaba ◽  
Sunita Sharma ◽  
Harleen Kaur ◽  
Pardeep Kaur

Background: Thymol is a bioactive compound having many pharmacological activities. Objective: The present study was carried out to evaluate the fungi toxic effects of thymol and derivatives against phytopathogenic fungi of maize. Method: Thymol was derivatized to get formylated thymol, Mannich bases, and imine derivatives. All the synthesized thymol derivatives were characterized by their physical and spectral properties. Synthesized thymol derivatives were screened for their in vitro antifungal effects using poisoned food technique against three maize pathogenic fungi namely Fusarium moniliforme, Rhizoctonia solani and Dreschlera maydis. Results: Thymol and formylated thymol showed promising results for control of D. maydis with ED50 values less than standard carbendazim and comparable to standard mancozeb. These two compounds were further evaluated for control of D. maydis causative maydis leaf blight disease on maize plants grown in the field during the Kharif season (June to October) 2018. Conclusion: Thymol exhibited significant control of maydis leaf blight disease of maize and emerged as a potential alternative to synthetic fungicides used in cereal crops.


2012 ◽  
Vol 37 (4) ◽  
pp. 233-238 ◽  
Author(s):  
Lucilene P. Lopes ◽  
Admiltom G. Oliveira Jr ◽  
Jamile P.O. Beranger ◽  
Cíntia G. Góis ◽  
Fernanda C.S. Vasconcellos ◽  
...  

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