scholarly journals Antifungal Activity Evaluation of Trichoderma Species Against Fusarium Wilt and Alternaria Leaf Blight of Sesame (Sesame indicum L.) Under in vitro Conditions

2020 ◽  
Vol 13 (2) ◽  
pp. 139-151
Author(s):  
Hadis Goshu ◽  
Afrasa Mulatu ◽  
Tesfaye Alemu
Keyword(s):  
Antifungal Activity ◽  
Fusarium Wilt ◽  
Leaf Blight ◽  
Trichoderma Species ◽  
Activity Evaluation ◽  
Alternaria Leaf Blight

scholarly journals Management of Alternaria leaf blight in tomato plants by mentha essential oil

2020 ◽  
Vol 56 (No. 3) ◽  
pp. 191-196
Author(s):  
Kahkashan Perveen ◽  
Najat A. Bokhari
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Mycelial Growth ◽  
Leaf Blight ◽  
In Vitro Test ◽  
Tomato Plants ◽  
Conidia Germination ◽  
Alternaria Leaf Blight

The essential oil obtained by the hydro-distillation of the leaves of Mentha arvensis Linnaeus was evaluated for its antifungal activity against the causal agent of the Alternaria blight of tomatoes, i.e., Alternaria alternata (Fries) Keissler. The antifungal activity of the mentha essential oil was assessed both in vitro and in vivo. The chemical composition of the mentha oil was also identified by GCMS analysis. The in vitro test revealed that the maximum inhibition in the mycelial growth (93.6%) and conidia germination (90.6%) was at the highest concentration (40 µL/mL), furthermore, it was found that the inhibition of the mycelial growth and conidia germination was dose dependent. The in vivo test proved that the application of the mentha essential oil (40 µL/mL) significantly increased the plant height (84.6%), fresh weight (81.5%) and dry weight (80.0%) when compared to the untreated tomato plants. The disease incidence was 3.5 in the untreated plants, while it was 0.93 for the mentha essential oil treated plants and was 0.08 in the carbendazim treated plants. The GC-MS analysis of the mentha essential oil identified 18 compounds in total, among which the percentage of menthol was the highest (69.2%). The mentha essential oil was successful in managing the Alternaria leaf blight in the tomato plants. Therefore, it can be explored further for the development of a natural fungicide.

scholarly journals In vitro antifungal activity of lime peels extract (Citrus aurantifolia (Cristm.) Swingle.) against Fusarium oxysporum Schlecht. em. Snyd. & Hans.

2019 ◽  
Vol 2 ◽  
Author(s):  
Rizki Wuri Widaryuni ◽  
Oktira Roka Aji
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Food Poisoning ◽  
Ethanol Extract ◽  
Positive Control ◽  
Optimum Concentration ◽  
Citrus Aurantifolia ◽  
Lime Peel

Fusarium wilt in plants is caused by  Fusarium oxysporum. Fusarium wilt disease prevention using synthetic fungicides cause various problems in the environment and human health, it is necessary to change to natural fungicides like lime peels. This study was conducted to determine the percentage of antifungal activity at the optimum concentration of ethanol extract of lime peel (Citrus aurantifolia) to inhibit the growth of Fusarium oxysporum. Lime peel extract was obtained from the maceration process using 96% ethanol solvent. Antifungal activity test uses a food poisoning method with mycelium diameter parameters that grow on the media. The concentration of ethanol extract of lime peels tested was 0%, 15%, 30%, 45%, and 60%, and the positive control used was synthetic antracol fungicide 0.3%. The results showed the optimum concentration of ethanol extract of lime peel to inhibit the growth of Fusarium oxysporum was a concentration of 15% with a percentage of antifungal activity is 56.34%. This is because the concentration is the lowest concentration that can inhibit the growth of Fusarium oxysporum with a strong level of activity.

scholarly journals Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1

2021 ◽  
pp. 466-478
Author(s):  
Mileidy Cruz Martín ◽  
Liliana Leyva ◽  
Mayra Acosta Suárez ◽  
Tatiana Pichardo ◽  
Idalmis Bermúdez Caraballoso ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Culture Filtrate ◽  
Bacillus Amyloliquefaciens ◽  
Biological Control Agent ◽  
Antifungal Effect ◽  
Antagonistic Microorganisms ◽  
Race 1

Introduction. Due to the absence of totally effective either economically viable chemical agents for the control of Fusarium wilt, the use of antagonistic microorganisms is of great interest since it could represent a more economically and ecologically sustainable alternative. Objective. To analyze the antifungal effect of the Bacillus amyloliquefaciens CCIBP-A5 strain against Fusarium oxysporum. Materials and methods. The work was carried out in the Laboratory of Applied Microbiology of the Instituto de Biotecnología de las Plantas, Cuba, between September 2017 and June, 2018. The in vitro and in vivo antifungal activity of its culture filtrate and cell against F. oxysporum has been assayed. Results. The results indicated that the metabolites present in the culture filtrate of B. amyloliquefaciens CCIBP-A5 significantly influenced the growth and morphology of the mycelium and the conidia. They also caused oxidative damage to the lipid molecules of F. oxysporum. In addition, this strain showed inhibitory effects on the development of the disease under controlled conditions. These aspects are key when selecting a bacterial candidate as a biological control agent. Conclusions. The results showed that the B. amyloliquefaciens CCIBP-A5 strain, isolated from Musa sp., had an in vitro antifungal effect against the vegetative and reproductive structures of Foc race 1 as well as on the Musa spp.-F. oxysporum interaction. This strain is suggested for the development of a bioproduct for Fusarium wilt management.

scholarly journals Association between field and in vitro reaction to alternaria leaf blight in sunflower genotypes

Helia ◽  
2003 ◽  
Vol 26 (38) ◽  
pp. 109-114 ◽  
Author(s):  
S.M. Shaik ◽  
R.L. Ravikumar
Keyword(s):  
Leaf Blight ◽  
Alternaria Leaf Blight

scholarly journals Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold

2018 ◽  
Vol 32 (2) ◽  
pp. 95-102 ◽  
Author(s):  
R. PRIYANKA ◽  
S. NAKKEERAN ◽  
I. ARUMUKA PRAVIN ◽  
A. S. KRISHNA MOORTHY ◽  
U. SIVAKUMAR ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Leaf Blight ◽  
Alternaria Leaf Blight ◽  
Subtilis Subsp

scholarly journals A Novel Antifungal Actinomycete Streptomyces sp. Strain H3-2 Effectively Controls Banana Fusarium Wilt

2021 ◽  
Vol 12 ◽  
Author(s):  
Niexia Zou ◽  
Dengbo Zhou ◽  
Yinglong Chen ◽  
Ping Lin ◽  
Yufeng Chen ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Wilt ◽  
Fermentation Broth ◽  
Membrane Integrity ◽  
Wilt Disease ◽  
Cell Membrane Integrity ◽  
Fusarium Wilt Disease ◽  
Fungal Phytopathogens ◽  
Environmentally Sound

Banana Fusarium wilt disease caused by Fusarium oxyspoum f. sp. cubense (Foc) seriously threatens the banana industry. Foc tropical race 4 (Foc TR4) can infect almost all banana cultivars. Compared with traditional physical and chemical practices, biocontrol strategy using beneficial microbes is considered as an environmentally sound option to manage fungal disease. In this study, a strain, H3-2, isolated from a non-infected banana orchard, exhibited high antifungal activity against Foc TR4. According to its morphological, physiological, and biochemical characteristics, the strain H3-2 was identified as Streptomyces sp. and convinced by the polymorphic phylogenic analysis of 16S rRNA sequences. Extracts of the strain H3-2 suppressed the growth and spore germination of Foc TR4 in vitro by destroying cell membrane integrity and mycelial ultrastructure. Notably, the strain and its extracts showed broad-spectrum antifungal activity against the selected seven fungal phytopathogens. Fourteen chemical compounds in the extracts were identified by gas chromatography–mass spectrometer (GC-MS), primarily phenolic compounds. Additional pot inoculation experiment demonstrated that the fermentation broth of the strain H3-2 promoted the growth of banana seedlings by efficiently inhibiting the spread of banana Fusarium wilt disease. This study demonstrated the potential application of the novel Streptomyces sp. H3-2 for the management of banana Fusarium wilt.

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