scholarly journals Template synthesis of macrocyclic complexes of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II): Spectroscopic, antibacterial and antifungal studies

2010 ◽  
Vol 75 (6) ◽  
pp. 763-772 ◽  
Author(s):  
Dharam Singh ◽  
Vandna Malik ◽  
Ramesh Kumar ◽  
Krishan Kumar
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Metal Ion ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
Carbonyl Oxygen ◽  
Macrocyclic Complexes ◽  
Spectral Studies ◽  
Distorted Octahedral Geometry ◽  
Divalent Metal Ions

A new series of macrocyclic complexes of the type [M(C17H14N4O2)X2], where M = Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and X = Cl-, NO3-, CH3COO-was synthesized by the condensation reaction of malonyldihydrazide with benzil in the presence of divalent metal ions. The complexes were characterized with the aid of elemental analyses, conductance measurements, magnetic susceptibilities, electronic, NMR and infrared spectral studies. On the basis of these studies, a six coordinate distorted octahedral geometry, in which two nitrogen and two carbonyl oxygen atoms are suitably placed for coordination towards metal ion, is proposed for all the complexes. The complexes were tested for their in vitro antibacterial activity and antifungal activities. The minimum inhibitory concentration shown by these complexes were compared with the minimum inhibitory concentration shown by standard drugs.

scholarly journals Synthesis and Characterization of Biologically Active 10-Membered Tetraazamacrocyclic Complexes of Cr(III), Mn(III), and Fe(III)

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Dharam Pal Singh ◽  
Vandna Malik ◽  
Ramesh Kumar
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Metal Ion ◽  
Inhibitory Concentration ◽  
Macrocyclic Ligand ◽  
Far Infrared ◽  
Carbonyl Oxygen ◽  
Biologically Active ◽  
Spectral Studies ◽  
Bacterial Strains

A new series of macrocyclic complexes of type [M(TML)X]X2; where M = Cr(III), Mn(III), or Fe(III); TML is tetradentate macrocyclic ligand and X = Cl−1, NO3−1, CH3COO−1 for Cr(III), Fe(III), and X = CH3COO−1 for Mn(III) has been synthesized by template condensation of succinyldihydrazide and glyoxal. The complexes have been formulated as [M(TML)X]X2 due to 1:2 electrolytic natures of these complexes as shown by conductivity measurements. The complexes have been characterized with the help of elemental analyses, molar conductance, electronic, infrared, far infrared spectral studies and magnetic susceptibilities. On the basis of these studies, a five-coordinate distorted square-pyramidal geometry, in which two nitrogens and two carbonyl oxygen atoms are suitably placed for coordination toward the metal ion, has been proposed for all the complexes. The complexes were tested for their in vitro antibacterial activity. Some of the complexes showed remarkable antibacterial activities against some selected bacterial strains. The minimum inhibitory concentration shown by these complexes was compared with minimum inhibitory concentration shown by some standard antibiotics like linezolid and cefaclor.

scholarly journals Synthesis, Characterization and Antifertility Activity of New Unsymmetrical Macrocyclic Complexes of Tin(II)

2000 ◽  
Vol 7 (5) ◽  
pp. 237-243 ◽  
Author(s):  
Kripa Sharma ◽  
S. C. Joshi ◽  
R. V. Singh
Keyword(s):  
Metal Ion ◽  
Amino Nitrogen ◽  
Pathogenic Fungi ◽  
Ring Expansion ◽  
Macrocyclic Complexes ◽  
Molar Ratio ◽  
Spectral Studies ◽  
Testicular Sperm ◽  
Sperm Density

A new series of unsymmetrical macrocyclic complexes of tin(ll) has been prepared by the template process using bis(3-oxo-2-butylidene)propane-1,3-diamine as precursor. This affords a method to synthesize these complexes with various ring sizes. The tetradentate macrocyclic precursor [N4mL] reacts with SnCl2 and different diamines in a 1:1:1 molar ratio in refluxing methanol to give complexes of the type [Sn(N4mL)Cl2]. The ring expansion has been achieved by varying the diamine between the two diacetyl amino nitrogen atoms. The macrocyclic precursor and its metal complexes have been characterized on the basis of elemental analysis, molar conductance, molecular weight determinations, IR, H1 NMR,C13 NMR, Sn119 NMR and electronic spectral studies. An octahedral geometry around the metal ion is suggested for these complexes. On the basis of molecular weights and conductivity measurements, their monomeric and non-electrolytic nature has been confirmed. The precursor and complexes have been screened in vitro against a number of pathogenic fungi and bacteria to assess their growth inhibiting potential. The testicular sperm density and testicular sperm morphology, sperm motility, density of cauda epididymal spermatozoa and fertility in mating trails and biochemicals parameters of reproductive organs have been examined and discussed.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

Synthesis,Characterization and Evaluation of Biological Activity of Sulfonylhydrazide-Schiff Base" (E)- N’-(2,5-dimethoxybenzalidene) naphthalene-2-sulfonohydrazide"

2020 ◽  
Vol 8 (1) ◽  
pp. 01-11
Author(s):  
Sameer Amereih ◽  
Abd Daraghmeh ◽  
Ismail Warad ◽  
Mohammed Al-Nuri
Keyword(s):  
Schiff Base ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
Gram Negative Bacteria ◽  
Biological Applications ◽  
Bacteriostatic Effect ◽  
1H And 13C Nmr ◽  
13C Nmr Analysis ◽  
Ft Ir

Condensation reaction of naphthalene -2-Sulfonylhydrazide, as starting material with 2,5-dimethoxy benzaldehyde was used to produce (E)-N’-(2,5-dimethoxybenzalidene)naphthalene-2-sulfonohydrazide. The Schiff base product was isolated, purified and then spectrally characterized via UV-Vis, GC/MS, FT-IR, 1H and 13C NMR analysis, where strong evidences confirmed the formation of the desired product. Antimicrobial activity of Schiff base product was evaluated in vitro against several types of bacteria such as: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and MRSA by Minimum Inhibitory Concentration (MIC) test using tetracycline (TE) as a standard antibiotic. The tests showed a promising bacteriostatic effect of this compound against gram negative bacteria such as P. aeruginosa and K. pneumoniae, such character is valuable for biological applications.

scholarly journals THE EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC 43718

2017 ◽  
Vol 10 (17) ◽  
pp. 26
Author(s):  
Khodijah Khodijah ◽  
Ratna Farida ◽  
Nurtami Soedarsono
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Spectrophotometric Analysis ◽  
Propolis Extract ◽  
Post Exposure ◽  
Colony Forming Units

Objective: This experiment aimed to analyze the effect of propolis extract and propolis containing candies on the growth of Aggregatibacter actinomycetemcomitans using spectrophotometric analysis and colony-forming units (CFU) counts.Methods: After A. actinomycetemcomitans were exposed to propolis extract and candies, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined with spectrophotometry and post-exposure colony counting.Results: The MIC of propolis extract against A. actinomycetemcomitans was determined to be 10%, and the MBC was 20%. A decrease in the total CFU count of A. actinomycetemcomitans was observed after propolis extract and candy exposure.Conclusions: Propolis extract and propolis candies were effective in inhibiting the growth of A. actinomycetemcomitans ATCC 43718 in vitro.

scholarly journals In VitroActivity of Omadacycline againstChlamydia pneumoniae

2018 ◽  
Vol 63 (2) ◽  
pp. e01907-18 ◽  
Author(s):  
Stephan A. Kohlhoff ◽  
Natalia Huerta ◽  
Margaret R. Hammerschlag
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Chlamydia Pneumoniae ◽  
Inhibitory Concentration ◽  
Content Type

ABSTRACTThein vitroactivities of omadacycline, azithromycin, doxycycline, moxifloxacin, and levofloxacin were tested against 15 isolates ofChlamydia pneumoniae. The minimum inhibitory concentration at which 90% of the isolates ofC. pneumoniaewere inhibited by omadacycline was 0.25 μg/ml (range, 0.03 to 0.5 μg/ml).

Activity of myxin against Ceratocystis ulmi

1969 ◽  
Vol 15 (1) ◽  
pp. 133-135 ◽  
Author(s):  
E. A. Peterson
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Liquid Culture ◽  
Inhibitory Concentration ◽  
In Vitro Tests ◽  
Strong Inhibition ◽  
Solid Media ◽  
Highly Sensitive ◽  
Paper Disc ◽  
Disc Assay

Eight strains of Ceratocystis ulmi originating from different locations and host species were found to be highly sensitive to the antibiotic myxin in in vitro tests. By paper disc assay, amounts as low as 0.5–1.0 μg caused strong inhibition of the fungus on solid media. The minimum inhibitory concentration in liquid culture was 0.2 μg/ml and levels of antibiotic above this concentration proved to be fungicidal.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals In vitro activities of Traganum nudatum and Mentha pulegium extracts combined with amphotericin B against Candida albicans in production of hydrolytic enzymes

2020 ◽  
Author(s):  
Ikram Tefiani ◽  
Sidi Mohammed Lahbib Seddiki ◽  
Moustafa Yassine Mahdad
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Culture Media ◽  
Hydrolytic Enzymes ◽  
Normal Flora ◽  
Mentha Pulegium ◽  
Hydrolytic Activities

Background and Purpose: Candida albicans is an important microorganism in the normal flora of a healthy subject; however, it has an expedient pathogenic character that induces hydrolytic virulence. Regarding this, the present study aimed to find an in vitro alternative that could reduce the virulence of this yeast. Materials and Methods: For the purpose of the study, the effect of amphotericin B (AmB) combined with the extract of Traganum nudatum (E1) or Mentha pulegium (E2) was evaluated against the hydrolytic activities of esterase, protease, and phospholipase. This effect was determined by calculating the minimum inhibitory concentration (MIC), used to adjust the extract/AmB mixtures in culture media. Results: The evaluated Pz values, which corresponded to the different enzymatic activities, showed a decrease in the hydrolytic activities of C. albicans strains after the addition of E1/AmB and E2/AmB combinations at descending concentrations (lower than the obtained MICs). Conclusion: Based on the findings, it would be possible to reduce the pathogenesis of this species without destabilizing the balance of the flora.

SYNTHESIS, SPECTRAL STUDIES, STRUCTURAL EVALUATION AND ANTIMICROBIAL ACTIVITY OF SOME NOVEL C R(III) COMPLEXES CONTAINING TETRADENTATE MACROCYCLIC LIGANDS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (06) ◽  
pp. 20-29
Author(s):  
S Shukla ◽  
◽  
S. Gautam ◽  
S Chandra ◽  
A. Kumar
Keyword(s):  
Metal Complexes ◽  
Metal Ion ◽  
Metal Salt ◽  
Condensation Reaction ◽  
Macrocyclic Ligand ◽  
Agar Plate ◽  
Macrocyclic Ligands ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Spectral Techniques

A string of novel coordination compounds of Cr(III) complexes have been derived and characterized from the macrocyclic ligands (L 1 -L 2 ) carried out by condensation reaction between ligands and the subsequent metal salt. The chemical composition of ligand was determined by analytical and spectral techniques i.e. elemental analysis, IR and Mass spectrocopy. Spectral techniques revealed tetradentate [N 4 ] the nature of ligand and its coordination mode to metal ion through nitrogen donor atoms. Metal complexes were characterized by elemental analyses, molar conductance, magnetic susceptibility measurements, IR, electronic spectra, ePR studies. The geometry of these complexes was ascertained by molecular modelling study by using Gaussian 09 program. All metal complexes were found to exhibit octahedral geometry around the metal ion. The newly synthesized macrocyclic ligands and metal complexes were subjected for antimicrobial screening to determine the inhibition and control against tested microorganisms, bacteria ( S.lutea , S.aureus, S.albus and E.coli ) and fungi ( A.fulviceps, U . hordei, A. niger and P.catinus ) by using disc diffusion method and agar plate technique, respectively. The experimental results suggest that metal complexes exhibit enhanced inhibition zone than free macrocyclic ligand.

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