scholarly journals Isolation of complexes formed between insulin-like growth factor-binding protein-3 and transferrin from the human serum

2012 ◽  
Vol 77 (5) ◽  
pp. 607-617 ◽  
Author(s):  
Goran Miljus ◽  
Miomir Petrovic ◽  
Olgica Nedic
Keyword(s):  
Growth Factors ◽  
Binding Protein ◽  
Biologically Active ◽  
Molecular Forms ◽  
Igf Binding Proteins ◽  
Igf Binding ◽  
The Relationship ◽  
Igfbp 3 ◽  
Insulin Like Growth Factors

Insulin-like growth factors (IGFs) play an important role in the regulation of cell growth, differentiation and metabolism. The amount of free, biologically active IGFs is regulated by the IGF-binding proteins (IGFBPs). IGFBP-3 is the most abundant binding protein and it is known to interact with other circulating proteins, including transferrin (Tf). In order to elucidate the possible role of IGF/IGFBP-3 in the iron metabolism, it is necessary to isolate IGFBP-3/Tf complexes. Several affinity-based techniques were employed. Results have shown that only double immunoprecipitation method with anti-Tf and anti-IGFBP-3 antibodies selectively separated complexes from other molecular forms, such as monomers, oligomers or fragments of IGFBP-3 and Tf. Isolated complexes can now be used to investigate the relationship between IGF/IGFBP-3 and iron, both in structural and metabolic t?rms.

Growth hormone (GH), insulin-like growth factors (IGFs), and IGF-binding protein-3 (IGFBP-3) in a child with Proteus syndrome

1994 ◽  
Vol 50 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Günter Rudolph ◽  
Werner F. Blum ◽  
Enno W. Jenne ◽  
Martin Schöning ◽  
Herbert Enders ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factors ◽  
Binding Protein ◽  
Proteus Syndrome ◽  
Igf Binding ◽  
Igf Binding Protein ◽  
Igfbp 3 ◽  
Insulin Like Growth Factors

Insulin-like growth factors and their binding proteins in domestic animals

1997 ◽  
Vol 64 (1) ◽  
pp. 1-15 ◽  
Author(s):  
K. L. Hossner ◽  
R. H. McCusker ◽  
M. V. Dodson
Keyword(s):  
Growth Factors ◽  
Binding Proteins ◽  
Initial Portion ◽  
Igf Binding Proteins ◽  
Hormone Effects ◽  
Igf Binding ◽  
Animal Growth ◽  
Metabolic Regulators ◽  
Insulin Like Growth Factors

AbstractInsulin-like growth factors (IGFs) and their binding proteins play an essential role in regulating animal growth and metabolism. The initial portion of the current review focuses on the physiological effects of the IGFs and delineates their role as regulators of animal growth and metabolism. The role of IGFs as mediators of growth hormone effects, as insulin-like metabolic regulators and as foetal growth regulators is discussed. The remainder of the review is devoted to the IGF binding proteins, their modulation of IGF action and their role in foetal and postnatal regulation of growth.

The role of growth hormone, insulin-like growth factors (IGFs), and IGF-binding proteins in experimental diabetic kidney disease

Metabolism ◽  
1995 ◽  
Vol 44 ◽  
pp. 67-71 ◽  
Author(s):  
Allan Flyvbjerg ◽  
Daniel Landau ◽  
Horacio Domene ◽  
Lute Hernandez ◽  
Henning Grønbæk ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factors ◽  
Kidney Disease ◽  
Diabetic Kidney Disease ◽  
Binding Proteins ◽  
Igf Binding Proteins ◽  
Diabetic Kidney ◽  
Igf Binding ◽  
Insulin Like Growth Factors
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