scholarly journals Tomato spotted wilt virus: One of the most destructive plant viruses

2008 ◽  
Vol 23 (3) ◽  
pp. 153-166 ◽  
Author(s):  
Branka Krstic ◽  
Aleksandra Bulajic ◽  
Ivana Djekic ◽  
Janos Berenji
Keyword(s):  
Molecular Biology ◽  
Host Range ◽  
Tomato Spotted Wilt Virus ◽  
Current Knowledge ◽  
Virus Transmission ◽  
Plant Viruses ◽  
Molecular Characteristics ◽  
Tomato Spotted Wilt ◽  
The World ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) has one of the largest host ranges among plant viruses and is widespread in all climates. TSWV is responsible for numerous epidemics in many parts of the world in different crops, mainly vegetables, tobacco and ornamentals. Its highly polyphagous nature, effectiveness of virus transmission by the thrips as its vectors, rapidity with which new variants arise, as well as difficulties in controlling the vectors make TSWV one of the most dangerous plant viruses. The ability of this virus to cause such severe losses on a broad range of crops, as well as its intriguing biological and molecular characteristics place TSWV amongst the most extensively studied plant viruses in the world at present. This paper provides a general overview of TSWV, encompassing all the major aspects of its biology and current knowledge on host range, symptomatology, molecular biology, vector relationship, control and diagnosis.

scholarly journals First Report of Tomato spotted wilt virus on a New Host Leuzea carthamoides in Bulgaria and the World

Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1258-1258 ◽  
Author(s):  
B. Dikova ◽  
N. Petrov ◽  
A. Djourmanski ◽  
H. Lambev
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Plant Viruses ◽  
Rt Pcr ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
The World ◽  
Systemic Symptoms ◽  
Wilt Virus ◽  
The Town ◽  
Das Elisa

The Siberian plant Leuzea carthamoides or maral root was introduced to Europe as a medicinal crop. Tomato spotted wilt virus (TSWV), genus Tospovirus, family Bunyaviridae, caused a harmful outbreak on L. carthamoides in central Bulgaria near the town of Kazanluk in 2009. In 2011, TSWV was identified on young sprouts from the rootages of L. carthamoides in the same place near the town of Kazanluk, Bulgaria, by means of indicator (test) plants, double antibody sandwich (DAS)-ELISA, and reverse transcription (RT)-PCR. Disease symptoms were small yellow spots on the young leaves grown from the tested sprouts and distortions of the leaf lamina. The old leaves had large yellow spots and necrosis, without deformations. Most of those L. carthamoides plants with such symptoms died in the second and third year. The number of the plants in the plantations decreased 20 to 40% during the 3-year period and some of these losses were from the virus disease except the environment conditions. DAS-ELISA was carried out with polyclonal TSWV antiserum of LOEWE Biochemica, GmbH, Germany. We obtained positive extinction values ODλ 405nm 0.358 ± 0.091 compared to the negative 0.053 ± 0.016 and the positive control 0.510 at a confidential interval at P ≤ 0.05. TSWV symptoms were observed on the following indicator plants according to Antignus et al. (1) and DPV/412 (2): Chenopodium quinoa, Cucumis sativus, Datura stramonium, Nicotiana glutinosa, N. rustica, N. tabacum cv. Samsun NN, and Petunia hybrida. TSWV caused on C. quinoa and on cotyledons of C. sativus cv. Delikates local chlorotic lesions only. In this TSWV differed from CMV because CMV caused systemic mosaic symptoms. Local small necrotic lesions and no systemic symptoms were observed on P. hybrida. We noticed systemic symptoms caused from TSWV on D. stramonium, N. glutinosa, N. rustica, and N. tabacum cv. Samsun NN. The systemic symptoms were chlorotic spots, concentric ring spots, and line patterns proceeding to necrosis. RT-PCR, adapted by Mumford et al. (3), was carried out on samples of L. carthamoides. Oligonucleotide primer sequences were used in accordance with Mumford et al. (3). The DNA fragment was visualized by UV trans-illumination. A fragment of the TSWV genome with a length of 276 base pairs was found in three young L. carthamoides leaf samples taken from the sprouts (marker 100 bp). The PCR fragment was sequenced and deposited to NCBI with GenBank Accession No. KC918808. PCR master mix without RNA template was used as a negative control. L. carthamoides is a newly established TSWV host in the world. To our knowledge, this is the first report of TSWV in L. carthamoides identified by RT-PCR. References: (1) Y. Antignus et al. Phytoparasitica 25:319, 1997. (2) R. Kormelink. Descriptions of Plant Viruses, p. 412, 2005. (3) R. A. Mumford et al. J. Virol. Methods 57:109, 1996.

Molecular biology of Tomato spotted wilt virus : An update

2013 ◽  
Vol 15 (02) ◽  
pp. 71-80 ◽  
Author(s):  
Saurabh Kulshrestha ◽  
Anshul Sharma ◽  
Chandrika Attri Seth
Keyword(s):  
Molecular Biology ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Specificity of accumulation and transmission of tomato spotted wilt virus (TSWV) in two genera, Frankliniella and Thrips (Thysanoptera: Thripidae)

2004 ◽  
Vol 94 (6) ◽  
pp. 501-507 ◽  
Author(s):  
T. Inoue ◽  
T. Sakurai ◽  
T. Murai ◽  
T. Maeda
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Virus Transmission ◽  
Leaf Disc ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Accumulation ◽  
Accumulation Pattern ◽  
N Protein ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Elisa Titre

AbstractThe accumulation and transmission of tomato spotted wilt virus (TSWV) was examined in second instar larvae and adults of two thrips genera, Frankliniella and Thrips. The species tested were F. occidentalis (Pergande), F. intonsa (Trybom), T. tabaciLindeman, T. setosus Moulton, T. palmi Karny and T. hawaiiensis (Morgan). In a standard petunia leaf disc assay, the efficiencies of TSWV transmission by two species of Frankliniella were higher than those of any Thrips species in the adult stage. A triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) showed that large amounts of the TSWV-nucleocapsid (N) protein were present in the ELISA-positive larvae of each species, with the exception of T. palmi. The ELISA titre of and the proportion of virus-infected individuals of the two Frankliniella species increased or did not significantly change from the larval to the adult stages, whereas those of the four Thrips species decreased significantly. These results show that the specificity of virus transmission by adult thrips is probably affected by the amount of viral N protein accumulation in the adults and that the accumulation pattern from the larval to the adult stages is in between the two genera tested in the present study.

scholarly journals THE MOST COMMON DISEASES OF ORNAMENTAL PLANTS IN THE ENTITY OF REPUBLIC OF SRPSKA (BOSNIA AND HERZEGOVINA)

AGROFOR ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Vojislav TRKULJA ◽  
Bojana ĆURKOVIĆ ◽  
Jelena VASIĆ ◽  
Bojana VUKOVIĆ ◽  
Gordana BABIĆ ◽  
...  
Keyword(s):  
Ornamental Plants ◽  
Plant Viruses ◽  
Plant Diseases ◽  
Impatiens Necrotic Spot Virus ◽  
Molecular Characteristics ◽  
Bacterial Diseases ◽  
Tomato Spotted Wilt ◽  
Alternaria Sp ◽  
Wilt Virus

During five year period (2011-2015) on territory of Republic of Srpska thecontinuous monitoring was carried out on different ornamental flowers for thepresence of plant diseases. For laboratory analysis following species Alyssum sp.,Aster sp., Bacopa sp., Begonia sp., Bellis perennis, Calceolaria sp.,Chrysanthemum sp., Cyclamen sp., Dahlia sp., Dianthus sp., Fuchsia sp., Gazaniasp., Gloxinia sp., Lobelia sp., Myosotis sp., Pelargonium sp., Petunia sp.,Petuniaxhybrida, Phlox sp., Plectranthus sp., Portulaca grandiflora, Primula sp.,Ranunculus sp., Rusmarinus officinalis, Salvia sp., Sansevaria sp., Tagetes sp.,Impatiens sp., Verbena sp. and Viola sp. were taken. Determination of causalpathogens was carried out on the basis of studying their morphological, pathogenic,biochemical and physiological, serological and molecular characteristics. The mostcommon causal pathogens were Alternaria sp., Botrytis sp., Golvonomyces sp.,Pestalotia sp., Phomopsis sp., Peronospora sp., Phytophtora sp., Puccinia sp. andSeptoria sp. Also, in much less extent, bacterial and viruses diseases were present.The most common bacterial diseases appear to be genera Pseudomonas andErwinia, while the most frequent plant viruses were Tomato spotted wilt virus(TSWV) and Impatiens necrotic spot virus (INSV).

scholarly journals Characterization and Establishment of a Recombinase Polymerase Amplification Assay for Rapid Detection of Tomato Spotted Wilt Virus in Pepper

2021 ◽  
Author(s):  
Kaiqiang Hao ◽  
Ming Gu ◽  
Miaoren Yang ◽  
Xinran Gao ◽  
Zihao Xia ◽  
...  
Keyword(s):  
Coat Protein ◽  
Phylogenetic Tree ◽  
Rapid Detection ◽  
Northeast China ◽  
Tomato Spotted Wilt Virus ◽  
Plant Viruses ◽  
Recombinase Polymerase Amplification ◽  
Liaoning Province ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV) is one of the most economically destructive and scientifically challenging plant viruses, which has seriously affected the production of commercial crops. At present, there is no effective strategy to control this virus. Therefore, there is an urgent need for a rapid and simple method to detect TSWV, which is of great significance to prevent its spread. In this study, an isolate of TSWV (TSWV-LNTL) infecting pepper from Liaoning Province of northeast China was obtained. A phylogenetic tree based on neighbor-joining using coat protein (CP) gene was established. A rapid method for detecting TSWV by recombinase polymerase amplification (RPA) was established. The phylogenetic tree based on the nucleotide sequences of coat protein (CP) genes of different TSWV isolates showed that the genetic relationship of TSWV-LNTL was most closely related to that of TSWV-LX-Lettuce-12 (Yunnan) and TSWV-TSHL (Shandong) isolates in China. It can be finished at 39 °C for 20 min and then purified by heating at 65 °C for 10 min. The RPA primers were highly specific and no cross-reactivity was detected with other selected viruses infecting pepper. The results of sensitivity test revealed that the detection limit of RPA is 1.0 × 103 copies/μL, which was tenfold lower than that of PCR method. In addition, the RPA method was successfully applied to detect TSWV in field samples. These results reported the occurrence of TSWV on crop in Liaoning Province of northeast China and demonstrated that the established RPA assay provided an effective molecular diagnostic tool for the accurate and rapid detection of TSWV to prevent its spread.

Effects of Thrips Density, Mode of Inoculation, and Plant Age on Tomato Spotted Wilt Virus Transmission in Peanut Plants

2015 ◽  
Vol 44 (1) ◽  
pp. 136-143 ◽  
Author(s):  
A. Shrestha ◽  
S. Sundaraj ◽  
A. K. Culbreath ◽  
D. G. Riley ◽  
M. R. Abney ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Virus Transmission ◽  
Plant Age ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

scholarly journals Crystal structure of tomato spotted wilt virus GNreveals a dimer complex formation and evolutionary link to animal-infecting viruses

2020 ◽  
Vol 117 (42) ◽  
pp. 26237-26244
Author(s):  
Yoav Bahat ◽  
Joel Alter ◽  
Moshe Dessau
Keyword(s):  
Crystal Structure ◽  
Tomato Spotted Wilt Virus ◽  
Structural Integrity ◽  
Structural Data ◽  
Plant Viruses ◽  
Insect Vector ◽  
Virus Acquisition ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Negative Sense

Tospoviridaeis a family of enveloped RNA plant viruses that infect many field crops, inflicting a heavy global economic burden. These tripartite, single-stranded, negative-sense RNA viruses are transmitted from plant to plant by thrips as the insect vector. The medium (M) segment of the viral genome encodes two envelope glycoproteins, GNand GC, which together form the envelope spikes. GCis considered the virus fusogen, while the accompanying GNprotein serves as an attachment protein that binds to a yet unknown receptor, mediating the virus acquisition by the thrips carrier. Here we present the crystal structure of glycoprotein N (GN) from the tomato spotted wilt virus (TSWV), a representative member of theTospoviridaefamily. The structure suggests that GNis organized as dimers on TSWV’s outer shell. Our structural data also suggest that this dimerization is required for maintaining GNstructural integrity. Although the structure of the TSWV GNis different from other bunyavirus GNproteins, they all share similar domain connectivity that resembles glycoproteins from unrelated animal-infecting viruses, suggesting a common ancestor for these accompanying proteins.

scholarly journals Importance and epidemiology of tomato spotted wilt virus

Biljni lekar ◽  
2021 ◽  
Vol 49 (2) ◽  
pp. 148-157
Author(s):  
Marina Ćuk ◽  
Zagorka Savić ◽  
Renata Iličić ◽  
Ferenc Bagi
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Frankliniella Occidentalis ◽  
Wide Distribution ◽  
Plant Viruses ◽  
Agricultural Crops ◽  
Agricultural Producers ◽  
Larval Stages ◽  
Tomato Spotted Wilt ◽  
Wide Range ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is the most economically important plant viruses from genus Tospovirus. It has a polyphagous character and infects a wide range of very significant agricultural crops. Vectors of viruses are insects from order Thysanoptera (Thripidae) and till know eight species are known to transmit tospoviruses of which Frankliniella occidentalis is considered to be economically most important vector. TSWV is transmitted by thrips in a persistent and propagative manner. Relationship between vector and TSWV is very specific because vectors acquire the virus in the larval stages, while imago plays a key role in transmission of the virus. TSWV causes wide range of symptoms depending on host plant, external environmental conditions and type of viruses. In addition to affecting the fruit quality of cultivated crops, greatly reduces the yield to agricultural producers. Tomato is the most commonly attacked by TSWV, and after the symptoms manifested on leaves in the form of a bronze color, the virus was name. Protection of agricultural crops is very challenging and difficult due to wide distribution of viruse vectors, their hidden way of life as well as wide range of TSWV hosts.

scholarly journals First Report of Tomato Spotted Wilt Virus on Potatoes in Iran

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 442-442 ◽  
Author(s):  
R. Pourrahim ◽  
Sh. Farzadfar ◽  
A. A. Moini ◽  
N. Shahraeen ◽  
A. Ahoonmanesh
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Chenopodium Quinoa ◽  
Plant Viruses ◽  
Thrips Tabaci ◽  
Enzyme Linked Immunosorbent Assay ◽  
Leaf Extracts ◽  
Systemic Necrosis ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields of Firouzkoh Province, Iran, during the summer of 1998. Infected plants died before the end of the growing season. Necrosis was more severe in cv. Agria than in cvs. Ajaxs and Arinda. A high population of Thrips tabaci was observed in August and September. Tomato spotted wilt virus (TSWV) (1) was detected in affected potatoes by using specific TSWV-IgG (from Bioreba) in double-antibody sandwich enzyme linked immunosorbent assay and by indicator plant reactions. Mechanical inoculation of indicator plants with leaf extracts of symptomatic potatoes produce necrotic local lesions in Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Vicia faba, Vigna sinensis, Phaseolus aureus var. Gohar, P. vulgaris, and Petunia hybrida. The virus caused systemic necrosis in Capsicum frutescens, Datura stramonium, D. metel, Nicotiana glutinosa, N. rustica, and Trapaeolum majus, preceded by systemic chlorotic spots. TSWV was reported from ornamental crops in Tehran and Absard areas near to Firouzkoh province (2), but this is the first report of TSWV occurrence on potatoes in Iran. References: (1) T. S. Ie. Descriptions of Plant Viruses. No. 39, 1970. (2) A. A. Moeini, et al. Iran. J. Plant Pathol. (In press.)

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