Optimization of cultivation medium composition for production of bioactive compounds effective against Penicillium sp.

Biological control is one of the best alternatives to pesticides as it avoids their weak points in plant disease control. In this study, the composition of cultivation medium for production of bioactive compounds by Bacillus subtilis ATCC 6633 was optimized. The produced bioactive compounds were tested against a phytopathogenic Penicillium sp. known for infesting different agricultural products and causing substantial crop losses. Antimicrobial activity assaying was carried out using the diffusion-disc method, and inhibition zone diameters were measured as direct indicators of antifungal activity. The response surface methodology (RSM) was used to evaluate the effects of different contents of initial nutrients (glycerol, NaNO2 and K2HPO4) in cultivation medium on inhibition zone diameter. Optimization was carried out using the desirability function method in order to maximize bioactive compounds yield and to minimize residual nutrients contents. The optimized concentrations of the selected nutrients in cultivation medium for production of bioactive compounds were: glycerol 20 g/l, NaNO2 1 g/l and K2HPO4 15 g/l.

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Optimization of the medium composition for production of antimicrobial substances by bacillus subtilis ATCC 6633

Acta periodica technologica ◽

10.2298/apt1748245r ◽

2017 ◽

pp. 245-259

Author(s):

Zorana Roncevic ◽

Jovana Djuran ◽

Jovana Grahovac ◽

Sinisa Dodic ◽

Aleksandra Ranitovic ◽

...

Keyword(s):

Bacillus Subtilis ◽

Sodium Nitrite ◽

Desirability Function ◽

Inhibition Zone ◽

Medium Composition ◽

Subtilis Atcc ◽

Antimicrobial Substances ◽

Nutritional Conditions ◽

Bacillus Subtilis Atcc ◽

Optimization Of Composition

In the effort to overcome the increase in antimicrobial resistance of different pathogens, natural products from microbial sources appear to be the most favorable alternative to current antibiotics. Production of antimicrobial compounds is highly dependent on the nutritional conditions. Hence, in order to achieve high product yields, selection of the media constituents and optimization of their concentrations are required. In this research, the possibility of antimicrobial substances production using Bacillus subtilis ATCC 6633 was investigated. Also, optimization of the cultivation medium composition in terms of contents of glycerol, sodium nitrite and phosphates was done. Response surface methodology and the method of desirability function were applied for determination of optimal values of the examined factors. The developed model predicts that the maximum inhibition zone diameters for Bacillus cereus ATCC 10876 (33.50 mm) and Pseudomonas aeruginosa ATCC 27853 (12.00 mm) are achieved when the initial contents of glycerol, sodium nitrite and phosphates were 43.72 g/L, 1.93 g/L and 5.64 g/L, respectively. The results of these experiments suggest that further research should include the utilization of crude glycerol as a carbon source and optimization of composition of such media and cultivation conditions in order to improve production of antimicrobial substances using Bacillus subtilis ATCC 6633.

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Biosynthesis of components with antifungal activity against Aspergillus spp. using Streptomyces hygroscopicus

Hemijska industrija ◽

10.2298/hemind130416009d ◽

2015 ◽

Vol 69 (2) ◽

pp. 201-208

Author(s):

Jelena Dodic ◽

Zorana Roncevic ◽

Jovana Grahovac ◽

Bojana Bajic ◽

Olivera Korolija

Keyword(s):

Antifungal Activity ◽

Soybean Meal ◽

Plant Protection ◽

Desirability Function ◽

Inhibition Zone ◽

Medium Composition ◽

Streptomyces Hygroscopicus ◽

Medium Components ◽

Optimal Medium

Losses of apple fruit during storage are mainly caused by fungal phytopathogens. Traditionally, postharvest fungal disease is controlled by the application of synthetic fungicides. However, the harmful impact on environment as well as human health largely limits their application. To reduce these problems in agrochemicals usage, new compounds for plant protection, which are eco-friendly, should be developed. The aim of this study is optimization of medium composition in terms of glucose, soybean meal and phosphates content, by applying response surface methodology, for the production of agents with antifungal activity against Aspergillus spp. For biosynthesis was used strain of Streptomyces hygroscopicus isolated from the environment. Experiments were carried out in accordance with Box-Behnken design with three factors on three levels and three repetitions in the central point. Antifungal activity of the obtained cultivation mediums against Aspergillus oryzae and Aspergillus niger was determined, in vitro, using the diffusion - disc method. For determination optimal medium components desirability function was used. Achieved model predicts that the maximum inhibition zone diameter (40.93 mm) against test microorganisms is produced when the initial content of glucose, soybean meal and phosphates are 47.77 g/l, 24.54 g/l and 0.98 g/l, respectively. To minimize the consumption of medium components and costs of effluents processing, additional three sets of optimization were made. The chosen method for optimization of medium components was efficient, relatively simple and time and material saving. Obtained results can be used for the further techno-economic analysis of the process to select optimal medium composition for industrial application.

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Optimization of cultivation medium for the production of antibacterial agents

Acta periodica technologica ◽

10.2298/apt1344217g ◽

2013 ◽

pp. 217-227

Author(s):

Jovana Grahovac ◽

Bojana Bajic ◽

Jelena Dodic ◽

Zorana Roncevic ◽

Aleksandar Jokic

Keyword(s):

Soybean Meal ◽

Pathogenic Bacteria ◽

Antibacterial Agents ◽

Desirability Function ◽

Inhibition Zone ◽

Antibiotic Activity ◽

Cultivation Medium ◽

Box Behnken Design ◽

Residual Sugar ◽

Streptomyces Spp

Optimization of the cultivation medium for production of antibiotic effective against pathogenic bacteria Staphylococcus aureus using strain of Streptomyces spp. isolated from the environment represents the aim of this study. After the biosynthesis, the medium was analyzed by determining residual sugar and nitrogen, and the antibiotic activity was determined using diffusion-disc method. Experiments were carried out in accordance with the Box-Behnken design, with three factors varied on three levels (glucose: 10.0, 30.0 and 50.0 g/L; soybean meal: 5.0, 15.0 and 25.0 g/L; phosphates: 0.5, 1.0 and 1.5 g/L) and for the optimization of selected parameters Response Surface Methodology was used. The obtained model with the desirability function of 0.985 estimates that the lowest amounts of residual sugar (0.89 g/L) and nitrogen (0.24 g/L) and the largest possible inhibition zone diameter (21.88 mm) that with its antibiotic activity against S. aureus creates the medium containing 10.0 g/L glucose, 5.0 g/L soybean meal and 1.04 g/L phosphates.

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Precision agriculture and geospatial techniques for sustainable disease control

Indian Phytopathology ◽

10.1007/s42360-021-00334-2 ◽

2021 ◽

Author(s):

Daniel P. Roberts ◽

Nicholas M. Short ◽

James Sill ◽

Dilip K. Lakshman ◽

Xiaojia Hu ◽

...

Keyword(s):

Big Data ◽

Disease Control ◽

Crop Production ◽

Plant Disease ◽

Production Systems ◽

Cropping Systems ◽

Data Driven ◽

Agricultural Community ◽

Plant Disease Control ◽

Crop Germplasm

AbstractThe agricultural community is confronted with dual challenges; increasing production of nutritionally dense food and decreasing the impacts of these crop production systems on the land, water, and climate. Control of plant pathogens will figure prominently in meeting these challenges as plant diseases cause significant yield and economic losses to crops responsible for feeding a large portion of the world population. New approaches and technologies to enhance sustainability of crop production systems and, importantly, plant disease control need to be developed and adopted. By leveraging advanced geoinformatic techniques, advances in computing and sensing infrastructure (e.g., cloud-based, big data-driven applications) will aid in the monitoring and management of pesticides and biologicals, such as cover crops and beneficial microbes, to reduce the impact of plant disease control and cropping systems on the environment. This includes geospatial tools being developed to aid the farmer in managing cropping system and disease management strategies that are more sustainable but increasingly complex. Geoinformatics and cloud-based, big data-driven applications are also being enlisted to speed up crop germplasm improvement; crop germplasm that has enhanced tolerance to pathogens and abiotic stress and is in tune with different cropping systems and environmental conditions is needed. Finally, advanced geoinformatic techniques and advances in computing infrastructure allow a more collaborative framework amongst scientists, policymakers, and the agricultural community to speed the development, transfer, and adoption of these sustainable technologies.

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Plant Disease Control Efficacy of Platycladus orientalis and Its Antifungal Compounds

Plants ◽

10.3390/plants10081496 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1496

Author(s):

Sohyun Bae ◽

Jae Woo Han ◽

Quang Le Dang ◽

Hun Kim ◽

Gyung Ja Choi

Keyword(s):

Disease Control ◽

Rice Blast ◽

Plant Disease ◽

Methanol Extract ◽

Crop Protection ◽

Ethyl Acetate Fraction ◽

Antifungal Compounds ◽

Control Efficacy ◽

Platycladus Orientalis ◽

Plant Disease Control

Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. In searching for natural alternatives to synthetic fungicides, we found that a methanol extract of the plant species Platycladus orientalis suppressed the disease development of rice blast caused by Magnaporthe oryzae. Through a series of chromatography procedures in combination with activity-guided fractionation, we isolated and identified a total of eleven compounds including four labdane-type diterpenes (1–4), six isopimarane-type diterpenes (5–10), and one sesquiterpene (11). Of the identified compounds, the MIC values of compounds 1, 2, 5 & 6 mixture, 9, and 11 ranged from 100 to 200 μg/mL against M. oryzae, whereas the other compounds were over 200 μg/mL. When rice plants were treated with the antifungal compounds, compounds 1, 2, and 9 effectively suppressed the development of rice blast at all concentrations tested by over 75% compared to the non-treatment control. In addition, a mixture of compounds 5 & 6 that constituted 66% of the P. orientalis ethyl acetate fraction also exhibited a moderate disease control efficacy. Together, our data suggest that the methanol extract of P. orientalis including terpenoid compounds has potential as a crop protection agent.

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