scholarly journals The effect of Nepeta rtanjensis essential oil on test micromycetes mycelia growth

2007 ◽  
pp. 173-177 ◽  
Author(s):  
Milica Ljaljevic-Grbic ◽  
Jelena Vukojevic ◽  
Marina Sokovic ◽  
Dragoljub Grubisic ◽  
Mihajlo Ristic
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Trichoderma Viride ◽  
Mycelia Growth ◽  
Alternaria Species ◽  
Alternaria Sp ◽  
Bipolaris Spicifera

The antifungal activity of Nepeta rtanjensis Diklic et Milojevic essential oil on mycelia growth has been performed by macrodilution method. The most efficient impact of N. rtanjensis essential oil on mycelia growth in vitro was found in Alternaria species with the same value of minimal inhibitory quantity (MIQ) of 0.6 ?l/ml. Bipolaris spicifera and Cladosporium cladosporoioides had MIQ values of 1.0 ?l/ml whereas Trichoderma viride with MIQ value of 1.6 ?l/ml showed the most efficient defense against the essential oil examined. The values of minimal fungicidal quantity (MFQ) in Alternaria sp. 2, B. spicifera and C. cladosporioides match the MIQ values whereas MFQ values in Alternaria sp. 1 is 0.8 ?l/ml, and in T. viride 1.8 ?l/ml.

scholarly journals Antifungal activity of Nepeta rtanjensis essential oil

2008 ◽  
Vol 73 (10) ◽  
pp. 961-965 ◽  
Author(s):  
Milica Grbic-Ljaljevic ◽  
Milos Stupar ◽  
Jelena Vukojevic ◽  
Marina Sokovic ◽  
Danijela Misic ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Trichoderma Viride ◽  
Cultivated Plants ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Alternaria Species ◽  
Agar Dilution ◽  
Bipolaris Spicifera ◽  
Main Component

The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklic & Milojevic was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aa,7a,7ab-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 ?g mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant.

Inhibitory effect of essential oil from Nepeta rtanjensis on fungal spore germination

2011 ◽  
Vol 6 (4) ◽  
pp. 583-586 ◽  
Author(s):  
Milica Grbić ◽  
Miloš Stupar ◽  
Jelena Vukojević ◽  
Dragoljub Grubišić
Keyword(s):  
Essential Oil ◽  
Trichoderma Viride ◽  
Fungal Spore ◽  
Inhibitory Effect ◽  
Cladosporium Cladosporioides ◽  
Germination Inhibition ◽  
Conidia Germination ◽  
Oil Concentration ◽  
Alternaria Species

AbstractThe ability of Nepeta rtanjensis essential oil to inhibit conidia germination of fungi was evaluated in vitro. Tested fungi included in research were Cladosporium cladosporioides, Trichoderma viride and two Alternaria species originally isolated from N. rtanjensis. The conidia of Cladosporium cladosporioides were most susceptible to the N. rtanjensis essential oil treatment, and the oil concentration of 0.1 µg ml−1 caused the maximum of conidia germination inhibition. The highest concentration used in experiment that caused the maximum of conidia germination inhibition was 0.6 µg ml−1 for Alternaria isolated from leaf surface of N. rtanjensis. The germ tube elongation of Alternaria isolates significantly decreased in response of different concentrations of oil used in experiment.

scholarly journals Antifungal Activity and Chemical Composition of Seven Essential Oils to Control the Main Seedborne Fungi of Cucurbits

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 104
Author(s):  
Marwa Moumni ◽  
Gianfranco Romanazzi ◽  
Basma Najar ◽  
Luisa Pistelli ◽  
Hajer Ben Amara ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
In Vivo Studies ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Mycelia Growth ◽  
Seedborne Fungi ◽  
Seedborne Pathogens

Essential oils represent novel alternatives to application of synthetic fungicides to control against seedborne pathogens. This study investigated seven essential oils for in vitro growth inhibition of the main seedborne pathogens of cucurbits. Cymbopogon citratus essential oil completely inhibited mycelial growth of Stagonosporopsis cucurbitacearum and Alternaria alternata at 0.6 and 0.9 mg/mL, respectively. At 1 mg/mL, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and two Origanum majorana essential oils inhibited mycelia growth of A. alternata by 54%, 71%, 68%, 36%, 90%, and 74%, respectively. S. cucurbitacearum mycelia growth was more sensitive to Lavandula essential oils, with inhibition of ~74% at 1 mg/mL. To determine the main compounds in these essential oils that might be responsible for this antifungal activity, they were analyzed by gas chromatography–mass spectrometry (GC-MS). C. citratus essential oil showed cirtal as its main constituent, while L. dentata and L. nobilis essential oils showed eucalyptol. The M. alternifolia and two O. majorana essential oils had terpinen-4-ol as the major constituent, while for L. hybrida essential oil, this was linalool. Thus, in vitro, these essential oils can inhibit the main seedborne fungi of cucurbits, with future in vivo studies now needed to confirm these activities.

scholarly journals Phytochemical Investigation and In-vitro Antifungal Activity of Essential Oil from the Roots of Selinum vaginatum C. B. Clarke

2019 ◽  
pp. 1-11
Author(s):  
Pawan Kumar ◽  
Sanjay Kumar ◽  
Mohammad S. Javed ◽  
A. K. Tewari ◽  
K. S. Bisht
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Investigation ◽  
Mycelia Growth ◽  
Ic50 Values ◽  
Minor Portion ◽  
In Vitro Antifungal Activity

Aim: The study was to evaluate the phytogenic chemical compounds and Antifungal Activity of essential oil from roots of Selinum vaginatum C.B. Clarke, growing in the Himalayan region of Jammu & Kashmir. Methodology: The essential oil was analyzed by Gas Chromatography & Gas Chromatography-Mass Spectrometry in relation with their Kavot indices and mass spectra. Results: The oil was found completely dominated by oxygenated sesquiterpenoids (71.9%) which includes 14-hydroxy-δ-cadinene (37.5%), khusinol (20.7%), viridiflorol (8.0%), acorenone -B (4.2%) and 14-oxy-α-muurolene (1.1%) whereas δ-cadinene (8.9%), α-copaene (6.8%), germacrene-A (2.5%), and β-caryophyllene (1.3%) were the major compounds among sesquiterpenoids. Monoterpenoids constituted as the minor portion (3.8%) of essential oil. The oil was found almost free from oxygenated monoterpenoids (0.2%). The roots of S. vaginatum are used in folk lore medicines in Jammu & Kashmir. The oil from the roots showed marked antifungal activity. The oil had shown 100% mycelia growth inhibition against A. tenuis, C. graminicola, R. solani and S. sclerotiorum at a concentration of 500 µg/ml, 2000 µg/mL, 2000 µg/mL and 300 µg/mL respectively. However F. oxysporum was found less susceptible to the root oil of S. vaginatum. The IC50 values showed a range from 57.4 µg/mL–74.7 µg/mL as compared to standard fungicides with IC50 values 32.8 µg/mL–98.6 µg/mL. The spore germination inhibition test revealed the root oil as a potent inhibitor with IC50 values as 201.4 µg/mL, 414.7 µg/mL and 784.7 µg/mL for A. tenuis, C. graminicola and F. oxysporum. Conclusion: Our study showed that14-hydroxy-δ-cadinene (37.5%), khusinol (20.7%), & viridiflorol (8.0%) are the major components in this oil and possessed potent antifungal activity against test fungal strain, respectively.

scholarly journals Succeptibility of some fungi to Boswellia carteri Birdw. essential oil

2016 ◽  
pp. 19-27 ◽  
Author(s):  
Milos Stupar ◽  
Marina Kostic ◽  
Zeljko Savkovic ◽  
Nikola Unkovic ◽  
Milica Ljaljevic-Grbic ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Pathogenic Fungi ◽  
Positive Control ◽  
Stachybotrys Chartarum ◽  
Commercial Sample ◽  
Mycelia Growth ◽  
Susceptible Isolate ◽  
Oil Concentration

Antifungal activity of commercial sample of Boswellia carteri essential oil against selected micromycetes was evaluated in vitro using a microatmosphere method. When compared with biocide Sanosil S003, used as positive control, the tested essential oil showed moderate antifungal activity. The most susceptible fungi to oil treatment were Stachybotrys chartarum and Trichotecium roseum. For both fungi, mycelia growth inhibition of 85% was recorded at oil concentration of 100 ?L mL-1. The tested essential oil caused inhibition of S. chartarum sporulation as well as depigmentation of conidi?, which is very significant since melanin contributes to virulence, survival and endurance of pathogenic fungi spores. Aspergillus niger was the least susceptible isolate to essential oil treatment. Mycelial growth of this fungus was not inhibited by any oil concentrations used in the experiment.

scholarly journals Antifungal activity of nanoemulsions encapsulating oregano (Origanum vulgare) essential oil: in vitro study and application in Minas Padrão cheese

2018 ◽  
Vol 49 (4) ◽  
pp. 929-935 ◽  
Author(s):  
Carolina M. Bedoya-Serna ◽  
Gustavo C. Dacanal ◽  
Andrezza M. Fernandes ◽  
Samantha C. Pinho
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
In Vitro Study ◽  
Vitro Study ◽  
Origanum Vulgare

scholarly journals Chemical composition and antifungal activity of essential oils from four Asteraceae plants grown in Egypt

2018 ◽  
Vol 73 (7-8) ◽  
pp. 313-318 ◽  
Author(s):  
Rose Vanessa Bandeira Reidel ◽  
Simona Nardoni ◽  
Francesca Mancianti ◽  
Claudia Anedda ◽  
Abd El-Nasser G. El Gendy ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
First Time

Abstract The objective of the present paper was the assessment of the chemical composition of the essential oils from four Asteraceae species with a considerable food, medicinal, and agricultural value, collected in Egypt, together with their in vitro inhibitory activity against molds and yeasts. The essential oil of Launaea cornuta flowers was also evaluated for the first time, but because of its very low yield (<0.01%), no antifungal test was performed.

scholarly journals Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tamirat Bekele Beressa ◽  
Serawit Deyno ◽  
Paul E. Alele
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Cryptococcus Neoformans ◽  
Antioxidant Properties ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

scholarly journals In Vitro Antifungal Activity of Polysulfides-Rich Essential Oil of Ferula Latisecta Fruits against Human Pathogenic Dermatophytes

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Abdolmajid Fata ◽  
Bahareh Emami ◽  
Bibi Mohadeseh Jalalzadeh Shahri ◽  
Bibi Sedigheh Fazly Bazzaz
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution ◽  
Sulfur Containing ◽  
Type Strains ◽  
In Vitro Antifungal Activity

The increase in dermatophytoses and the fact that some patients do not respond well to therapy make it necessary to find new antifungal agents. As part of our ongoing studies on medicinal plants from Iran, we studied antidermatophytic activities of Ferula latisecta essential oil, which had shown considerable antifungal activity in preliminary antimicrobial screening. Antifungal activity was evaluated by determination of MIC values using the agar dilution method on type strains of Candida albicans and dermatophytes. The composition of the oil was characterized by GC and GC/MS analyses. The essential oil was rich in polysulfides (75.2%) and exhibited good activity against Trichophyton rubrum and T. verrucosom for about three weeks, with a MIC value 96 μg/mL. The oil showed antifungal activity, especially against dermatophytes, and the activity is probably related to the sulfur-containing components of the oil. This study has identified that the polysulfides-rich essential oil of Ferula latisecta fruits has activity against a range of human pathogenic dermatophytes, justifying future clinical trials to validate its use as a therapeutic alternative for dermatophytosis.

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