In Vitro Hatching of Ascaris suum Eggs

Sperm of the nematode, Ascaris suum, are amoeboid cells that do not require actin or myosin to crawl over solid substrata. In these cells, the role usually played by actin has been taken over by major sperm protein (MSP), which assembles into filaments that pack the sperm pseudopod. These MSP filaments are organized into multi-filament arrays called fiber complexes that flow centripetally from the leading edge of the pseudopod to the cell body in a pattern that is intimately associated with motility. We have characterized structurally a hierarchy of helical assemblies formed by MSP. The basic unit of the MSP cytoskeleton is a filament formed by two subfilaments coiled around one another along right-handed helical tracks. In vitro, higher-order assemblies (macrofibers) are formed by MSP filaments that coil around one another in a left-handed helical sense. The multi-filament assemblies formed by MSP in vitro are strikingly similar to the fiber complexes that characterize the sperm cytoskeleton. Thus, self-association is an intrinsic property of MSP filaments that distinguishes these fibers from actin filaments. The results obtained with MSP help clarify the roles of different aspects of the actin cytoskeleton in the generation of locomotion and, in particular, emphasize the contributions made by vectorial assembly and filament bundling.

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Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f090101 ◽

2011 ◽

Vol 9 (1) ◽

pp. 1-6

Author(s):

OKKIE MHARGA SENTANA ◽

SRI HARYATI ◽

YUL MARIYAH

Keyword(s):

Linear Regression ◽

Ascaris Suum ◽

Linear Regression Analysis ◽

Negative Relationship ◽

Sampling Technique ◽

Ethanol Extract ◽

Probit Analysis ◽

Control Group ◽

Ocimum Americanum

Sentana OM, Haryati S, Mariyah Y. 2011. Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro. Biofarmasi 9: 1-6. This research aimed to examine the relationship between the ethanol extract of basil leaf against Ascaris suum mortality rate in vitro. This study was a laboratory experiment with a research design of the post-test with controlled group design. The samples were Ascaris suum Goeze that still actively moved and obtained from pig intestines. The sampling technique used was a purposive sampling by equating the type and the length of worms, and it did not distinguished for the sex of worms. The subjects were divided into five groups, each group consisted of 5 worms and repeated in 6 times. The control group contained 25 mL of physiological saline solution and 25 mL pyrantel pamoate 5 mg/mL. Three other groups contained basil leaves extracts consisted of various concentrations, i.e. 30%, 40% and 50%. The number of dead worms was counted every 2 hours until all the worms in the group died. The data were analyzed by using a linear regression analysis and a probit analysis. Simple linear regression statistical test showed a significance correlation at 0.000 with 0.837 correlation coefficient was negative, R square 0.701, in which the significant correlation <0.05 or the value of 37.565 with a significance of means, there was a negative relationship between the length of time of death of worms with large concentrations of basil extract. Analysis using a probit analysis obtained LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours. The ethanol extract of basil leaf could affect the death of A. suum in vitro with LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours.

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Influence of pH, Serum, and Cell Cultures on Development of Ascaris suum to Fourth Stage In vitro

Journal of Parasitology ◽

10.2307/3277649 ◽

1970 ◽

Vol 56 (2) ◽

pp. 238 ◽

Cited By ~ 8

Author(s):

Frank W. Douvres ◽

Francis G. Tromba

Keyword(s):

Cell Cultures ◽

Ascaris Suum ◽

Fourth Stage ◽

Influence Of Ph

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In vitro Anthelminthic Activity of Crude Methanolic Leaf Extract of Hyptis suaveolens (L.) Poit (Bush mint) on Ascaris suum.

Journal of Veterinary and Biomedical Sciences ◽

10.36108/jvbs/9102.20.0101 ◽

2019 ◽

Vol 2 (1) ◽

pp. 82-87

Author(s):

O. S Salami

Keyword(s):

Leaf Extract ◽

Ascaris Suum ◽

Positive Control ◽

Negative Control ◽

Phytochemical Analysis ◽

Egg Hatchability ◽

Anthelminthic Activity ◽

Life Cycle Stages ◽

Hyptis Suaveolens

Ascariasis is a neglected tropical disease prevalent in areas with poor hygiene and low standard of living in tropical countries. Hyptis suaveolens is been used traditionally around the world for various ailments. The aim of the study was to evaluate the anthelminthic activity of the methanolic leaf extract of the plant on the three life cycle stages of Ascaris suum synonymous to Ascaris lumbricoides. The phytochemical analysis of the extract was evaluated. The in-vitro anthelminthic activity of the methanolic leaf extract of Hyptis suaveolens on the egg hatchability and larva inhibition of Ascaris suum were assessed using Bizimenyara inhibition method, where 100 eggs were counted and incubated in 5 different plate-wells containing positive control, negative control and 3 concentrations (50, 70 and 100 mg/ml) of extract. The plate wells were brought out after incubation and content examined under the microscope. The effect of the methanolic leaf extract and pyrantel pamoate on cuticle peeling, motility and mortality were evaluated using adult worms. The egg hatchability and larval development after incubation for 48 h and 21 days significantly (p<0.05 for both) inhibited with 98% unhatched eggs and 95% undeveloped larva of A. suum respectively. There was significant (p<0.05) visible peeling of cuticles, reduced motility and mortality of the adult worms after 30, 12 and 80 minutes respectively. It can be concluded that H. suaveolens has anthelminthic activity on A. suum which may be attributed to some of the phytochemical constituents.

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