Lactoferrin Promotes Proliferation and Wound Healing of Human Gingival Fibroblasts -in vitro study

Abstract Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.

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In vitro study of surface alterations to polyetheretherketone and titanium and their effect upon human gingival fibroblasts

Journal of Prosthetic Dentistry ◽

10.1016/j.prosdent.2019.12.012 ◽

2021 ◽

Vol 125 (1) ◽

pp. 155-164 ◽

Cited By ~ 1

Author(s):

Maryam Gheisarifar ◽

Geoffrey A. Thompson ◽

Carl Drago ◽

Fahimeh Tabatabaei ◽

Morteza Rasoulianboroujeni

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Human Gingival Fibroblasts ◽

Gingival Fibroblasts

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PR528: Human gingival fibroblasts proliferation and attachment to different novel implant-abutment materials: an in vitro study

Journal Of Clinical Periodontology ◽

10.1111/jcpe.529_12915 ◽

2018 ◽

Vol 45 ◽

pp. 299-299

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Human Gingival Fibroblasts ◽

Gingival Fibroblasts ◽

Implant Abutment

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Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study

The Kaohsiung Journal of Medical Sciences ◽

10.1016/j.kjms.2015.03.005 ◽

2015 ◽

Vol 31 (6) ◽

pp. 303-308 ◽

Cited By ~ 3

Author(s):

Hye-Jin Lee ◽

Choong-Ho Choi

Keyword(s):

Sodium Fluoride ◽

In Vitro Study ◽

Vitro Study ◽

Human Gingival Fibroblasts ◽

Gingival Fibroblasts ◽

Bamboo Salt ◽

Anti Inflammatory

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Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study

Brazilian Dental Journal ◽

10.1590/0103-6440201300238 ◽

2015 ◽

Vol 26 (6) ◽

pp. 602-606 ◽

Cited By ~ 5

Author(s):

Rafaela S. R. e Lima ◽

Daiane C. Peruzzo ◽

Marcelo H. Napimoga ◽

Eduardo Saba-Chujfi ◽

Silvio Antonio dos Santos-Pereira ◽

...

Keyword(s):

Extracellular Matrix ◽

In Vitro Study ◽

Vitro Study ◽

Human Gingival Fibroblasts ◽

Soft Tissue Augmentation ◽

Biological Behavior ◽

Type Iii Collagen ◽

Gingival Fibroblasts ◽

Type Iii

Mucograft(r) is a resorbing porcine matrix composed of type I and type III collagen, used for soft tissue augmentation in guided tissue bony regeneration procedures. This in vitro study aimed to evaluate the biological behavior of Mucograft(r) in human gingival fibroblasts, as well as the ability of the matrix to induce production of extracellular matrix. Six resorbing Mucograft(r) matrices (MCG) were cut into 3 x 2 mm rectangles and 5 x 5 mm squares and were placed in 96- and 24-well plates, respectively. The control group (CTRL) consisted of cells plated on polystyrene without the MCG. After one, two, three and seven days, cell proliferation and viability were assessed using the Trypan exclusion method and MTT test, respectively. Type III collagen (COL 3A1) and vimentin (VIM) expression were also evaluated at 10 and 14 days, using Western blotting. Statistical analysis, using ANOVA with post hoc Bonferroni test, revealed that human gingival fibroblasts from MCG showed similar results (p>0.05) for proliferation and viability as the cells cultured on CTRL. After 14 days, a significant decrease in COL 3A1 expression (p<0.05) was observed when cultured with the MCG. VIM expression showed no significant difference at any time period (p>0.05). Although no increase in extracellular matrix secretion was observed in this in vitro study, Mucograft(r) presented cellular compatibility, being an option for a scaffold whenever it is required.

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