A Case of Lymphocutaneous Nocardiosis Caused by Nocardia brasiliensis-Early Diagnostic Availability of Gram Staining and Kinyoun Staining-

O isolamento e identificação do agente etiológico causador de infecção a partir de líquidos biológicos podem ser um fator crítico para a recuperação da saúde do paciente, pois estas infecções geralmente são graves e deixam seqüelas. Neste trabalho foram analisadas 82 amostras de diferentes líquidos biológicos, comparando os resultados da coloração de Gram com os das culturas, além de verificar incidência dos microrganismos isolados. O Gram apresentou sensibilidade e especificidade de 62,5% e 93,9% respectivamente. Pseudomonas aeruginosa, Escherichia coli e Staphylococcus aureus foram as bactérias mais isoladas. Concluiu-se que a Coloração de Gram pode ser uma ferramenta útil na análise dos líquidos biológicos, contribuindo no diagnóstico preliminar destas infecções. SENSITIVITY OF GRAM STAINING FOR EARLY DIAGNOSTIC OF INFECTIONS IN STERILE BODY SITES Abstract Isolation and identification of an etiologic agent from biological fluids can be a critic factor for the clinical outcome of the patient, because this infection can generally be severe and cause sequels. Eighty-two different samples of biological fluids were analyzed through the Gram staining method. The results of Gram were compared with those obtained with culture, and the incidence of isolated microorganisms was also analyzed. The Gram staining presented 62.5% and 93.9% of sensitivity and specificity, respectively. Among all types of isolated and identified bacteria, P. aeruginosa, E. coli and S. aureus were the commonest ones. It was concluded that the Gram staining could be a useful tool on analysis of biological fluids, contributing for the previous diagnostic of these infections.

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Dual stain kit for the microscopic gram classification of ocular infection bacteria

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147089 ◽

1993 ◽

Vol 51 ◽

pp. 248-249

Author(s):

Jacob S. Hanker ◽

Dale N. Holdren ◽

Kenneth L. Cohen ◽

Beverly L. Giammara

Keyword(s):

Contact Lenses ◽

Ocular Infection ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Gram Staining ◽

Ocular Infections ◽

Different Types ◽

Culture Positive ◽

Increased Susceptibility

Keratitis and conjunctivitis (infections of the cornea or conjunctiva) are ocular infections caused by various bacteria, fungi, viruses or parasites; bacteria, however, are usually prominent. Systemic conditions such as alcoholism, diabetes, debilitating disease, AIDS and immunosuppressive therapy can lead to increased susceptibility but trauma and contact lens use are very important factors. Gram-negative bacteria are most frequently cultured in these situations and Pseudomonas aeruginosa is most usually isolated from culture-positive ulcers of patients using contact lenses. Smears for staining can be obtained with a special swab or spatula and Gram staining frequently guides choice of a therapeutic rinse prior to the report of the culture results upon which specific antibiotic therapy is based. In some cases staining of the direct smear may be diagnostic in situations where the culture will not grow. In these cases different types of stains occasionally assist in guiding therapy.

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