721-P: Exercise Training Alters Subcutaneous Adipose Tissue Morphology in Obese Adults Even without Weight Loss

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Vol 68 (Supplement 1) ◽  
pp. 721-P
Author(s):  
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BENJAMIN J. RYAN ◽  
JENNA B. GILLEN ◽  
ALISON LUDZKI ◽  
MICHAEL W. SCHLEH ◽  
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BENJAMIN J. RYAN ◽  
CHIWOON AHN ◽  
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JEFFREY F. HOROWITZ

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Biomedicines ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 123 ◽  
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Macrophage infiltration in two subcutaneous adipose tissue depots and systemic low-grade inflammation were studied in post-obese (PO), obese (O), and control (C) subjects. Young males were recruited into PO: (n = 10, weight-loss avg. 26%, BMI: 26.6 ± 0.7, mean ±SEM kg/m2), O: (n = 10, BMI: 33.8 ± 1.0kg/m2) and C: (n = 10, BMI: 26.6 ± 0.6 kg/m2). PO and C were matched by BMI. Blood and abdominal and gluteal subcutaneous adipose tissue were obtained in the overnight fasted state. Plasma concentrations of IL-6 and CRP were higher (p < 0.05) in O than in PO and C, TNF-α was higher (p < 0.05) only in O compared to PO and IL-18 was similar between groups. The number of CD68+ macrophages was higher (p < 0.05) in the gluteal than the abdominal depot, and higher (p < 0.05) in O and PO compared to C in both depots. The content of CD163+ macrophages was similar between depots but was higher (p < 0.05) in PO compared to C and O in the gluteal depot. In post obese men with a long-term sustained weight loss, systemic low-grade inflammation was similar to non-obese controls despite a higher subcutaneous adipose tissue CD68+ macrophage content. Interestingly, the anti-inflammatory CD163+ macrophage adipose tissue content was consistently higher in post obese than obese and controls.


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