scholarly journals PERBEDAAN KEBERHASILAN TINGKAT POLIPLOIDISASI IKAN MAS (Cyprinus carpio Linn.) MELALUI KEJUTAN PANAS

2005 ◽  
Vol 10 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Akhmad Taufiq Mukti
Keyword(s):  
Data Analysis ◽  
Survival Rate ◽  
Heat Shock ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Shock Treatment ◽  
Initial Time ◽  
Hatching Rate ◽  
Heat Shock Treatment ◽  
The Common

The easiest, cheapest, simpliest and efficient polyploidization method was heat shock treatment. Each fish species has different tolerance either initial time, temperature or duration of heat shock. The aim of this study were to show and determine the heat shock effects on differentiation of hatching rate, abnormality, survival rate, and successful polyploidization of common carp. The method that used in this study was experiment. Treatment that used was heat shock 40° C during 1.5 minutes of common carp eggs to 3 and 29 minutes after fertilization. Ten replicates were carried out for each treatment. Parameters test were hatching rate, abnormality, survival rate and polyploidy induction by counting of nucleolus number. Data analysis that used was descriptive. The result of this study indicated that heat shock treatment influenced on hatching rate, abnormality, survival rate and polyploidy induction of the common carp polyploidization. Hatching rate of common carp, triploidy and tetraploidy were 22.63 ± 8.36 percent and 11.10 ± 8.60 percent, abnormality 13.81 ± 4.67 percent and 24.86 ± 8.37 percent, survival rate 52.64 ± 8.46 percent and 55.04 ± 8.15 percent and polyploidy induction 70 ± 7.07 percent and 60 ± 7.07 percent, respectively.

Heat-induced triploids in Brycon amazonicus: a strategic fish species for aquaculture and conservation

Zygote ◽  
2021 ◽  
pp. 1-5
Author(s):  
Nivaldo Ferreira do Nascimento ◽  
Rafaela Manchin Bertolini ◽  
Lucia Soares Lopez ◽  
Laura Satiko Okada Nakaghi ◽  
Paulo Sérgio Monzani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Heat Shock ◽  
Early Development ◽  
Fish Species ◽  
Survival Rates ◽  
Shock Treatment ◽  
Hatching Rate ◽  
Heat Shock Treatment ◽  
Control Survival ◽  
Ploidy Status

Summary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus.

The induction of Hsp70 synthesis by non-lethal heat shock confers thermotolerance and resistance to lethal ammonia stress in the common carp,Cyprinus carpio(Linn)

2013 ◽  
Vol 45 (10) ◽  
pp. 1706-1712 ◽  
Author(s):  
Yeong Yik Sung ◽  
Hon Jung Liew ◽  
Abol Munafi Ambok Bolong ◽  
Mohd. Effendy Abdul Wahid ◽  
Thomas H MacRae
Keyword(s):  
Heat Shock ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
The Common ◽  
Ammonia Stress ◽  
Hsp70 Synthesis

Effects of heat shock treatment on the survival rate of Lactobacillus acidophilus after freeze-drying

2020 ◽  
Vol 136 ◽  
pp. 109507
Author(s):  
Ni Zhen ◽  
Xiaoqun Zeng ◽  
Huijun Wang ◽  
Jing Yu ◽  
Daodong Pan ◽  
...  
Keyword(s):  
Survival Rate ◽  
Heat Shock ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Shock Treatment ◽  
Heat Shock Treatment

scholarly journals ANALISIS JUMLAH KROMOSOM PADA TRIPLOIDISASI IKAN MAS (Cyprinus carpio Linn) RAS PUNTEN DENGAN LAMA PERENDAMAN KEJUT SUHU PANAS YANG BERBEDA

2018 ◽  
Vol 1 (1) ◽  
pp. 59
Author(s):  
Rahmad Hendro Susilo ◽  
Farikhah Farikhah ◽  
Andi Rahmad Rahim
Keyword(s):  
Heat Shock ◽  
Cyprinus Carpio ◽  
Seed Quality ◽  
T Test ◽  
Effect Of Temperature ◽  
Shock Duration ◽  
Hatching Rate ◽  
Heat Shock Treatment ◽  
Triploid Fish

Goldfish (Cyprinus carpio Linn) punten breeds is a type of freshwater fish that has greatpotential in terms of quality and quantity. Seed production from generation to generation hasdecreased quality. An alternative effort was made to maintain the seed quality of geneticengineering with triploidization process using heat shock treatment. The heat shock can preventpolar body II from splitting on eggs during cleavage of meiosis II level resulting in 3Nchromosome or sterile fish. The purpose of this research is to analyze chromosome number intriploidization process of punten breeds fish and to analyze the effect of temperature shockduration on hatching rate of goldfish (Cyprinus carpio Linn) Punten Breeds. This research wasdescriptive research with 2 treatments and 3 replications in each treatment that was heat shocktreatment 40 ºC at minute 2 after fertilization with difference of heat shock duration 1,5 minutes(treatment A) and shock time temperature 2 minutes (treatment B). The parameters observedwere triploid success, hatching rate, survival rate, and growth of of goldfish (Cyprinus carpioLinn) Punten Breeds, analyzed by T-test with 95% confidence interval. The result of heat shockduration of 1.5 minutes showed the percentage of triploid fish 53,57% and shock timetemperature 2 minute 43,64%. The result of T-test of both treatments had no significant effect onall observation parameters but had varied relationship that was enough and inversely related toall observation parameters

scholarly journals Characterization of the thermotolerant cell. I. Effects on protein synthesis activity and the regulation of heat-shock protein 70 expression.

1988 ◽  
Vol 106 (4) ◽  
pp. 1105-1116 ◽  
Author(s):  
L A Mizzen ◽  
W J Welch
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Stress Proteins ◽  
Stress Protein ◽  
Shock Treatment ◽  
Heat Shock Treatment ◽  
Normal Medium ◽  
Growth Environment ◽  
Mammalian Cell Lines ◽  
C 30

Exposure of mammalian cells to a nonlethal heat-shock treatment, followed by a recovery period at 37 degrees C, results in increased cell survival after a subsequent and otherwise lethal heat-shock treatment. Here we characterize this phenomenon, termed acquired thermotolerance, at the level of translation. In a number of different mammalian cell lines given a severe 45 degrees C/30-min shock and then returned to 37 degrees C, protein synthesis was completely inhibited for as long as 5 h. Upon resumption of translational activity, there was a marked induction of heat-shock (or stress) protein synthesis, which continued for several hours. In contrast, cells first made thermotolerant (by a pretreatment consisting of a 43 degrees C/1.5-h shock and further recovery at 37 degrees C) and then presented with the 45 degrees C/30-min shock exhibited considerably less translational inhibition and an overall reduction in the amount of subsequent stress protein synthesis. The acquisition and duration of such "translational tolerance" was correlated with the expression, accumulation, and relative half-lives of the major stress proteins of 72 and 73 kD. Other agents that induce the synthesis of the stress proteins, such as sodium arsenite, similarly resulted in the acquisition of translational tolerance. The probable role of the stress proteins in the acquisition of translational tolerance was further indicated by the inability of the amino acid analogue, L-azetidine 2-carboxylic acid, an inducer of nonfunctional stress proteins, to render cells translationally tolerant. If, however, analogue-treated cells were allowed to recover in normal medium, and hence produce functional stress proteins, full translational tolerance was observed. Finally, we present data indicating that the 72- and 73-kD stress proteins, in contrast to the other major stress proteins (of 110, 90, and 28 kD), are subject to strict regulation in the stressed cell. Quantitation of 72- and 73-kD synthesis after heat-shock treatment under a number of conditions revealed that "titration" of 72/73-kD synthesis in response to stress may represent a mechanism by which the cell monitors its local growth environment.

Acute Toxicity of Synthetic Pyrethroid Cypermethrin on the Common Carp (Cyprinus carpio L.) Embryos and Larvae

2005 ◽  
Vol 13 (5) ◽  
pp. 451-458 ◽  
Author(s):  
Rahmi Aydın ◽  
Kenan Köprücü ◽  
Mustafa Dörücü ◽  
Sibel Şimşek Köprücü ◽  
Murat Pala
Keyword(s):  
Acute Toxicity ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Synthetic Pyrethroid ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
The Common
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