scholarly journals Epidemiology of Ovine Pasteurellosis in Lume District, East Shewa Zone of Oromiya Region, Ethiopia

2016 ◽  
Vol 1 (3) ◽  
pp. 1-9
Author(s):  
Jarso D
Keyword(s):  
Veterinary Medicine ◽  
Pasteurella Multocida ◽  
Addis Ababa ◽  
Open Access Journal ◽  
Lung Lesion ◽  
Fishery Resource ◽  
Indirect Haemagglutination ◽  
Nasal Swabs ◽  
Addis Ababa University ◽  
Pasteurella Trehalosi

Open Access Journal of Veterinary Science & Research ISSN: 2474 - 92 22 Epidemiology of Ovine Pasteurellosis in Lume District, East Shewa Zone of Oromiya Region, Ethiopia J Vet Sci Res Epidemiology of Ovine Pasteurellosis in Lume District, East Shewa Zone of Oromiya Region, Ethiopia Sadia H 1 , Abunna F 2 and Jarso D 2 * 1 Oromia Livestock and Fishery Resource Development Bureau, Addis Ababa, Ethiopia 2 Department of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia *Corresponding author: Jarso D, Department of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia, E - mail: [email protected] Abstract The study of ovine pasteurellosis was conducted in Lume districts, East Shoa Zone of Oromia region, Ethiopia to determine the prevalence of M annheimia haemolytica , Pasteurella trehalosi and Pasteurella multocida from nasal swabs (384), abattoir specimens (145 ), and the serotype diversity among the species from sheep sera (150). A total of 115 isolates of M. haemolytica , P. treha losi and P. multocida were isolated from nasal swabs of apparently health and clinically sick sheep and from pneumonic lungs. The M. haemolytica, P. trehalosi and Pasteurella multocida were isolated from the nasal swabs (11.2%), (7.6%) and (2.1) respective ly, whereas M. haemolytica and P. trehalosi were isolated from pneumonic lungs (11.7%) , ( 10.3 ) respectively. However, Pasteurella multocida was the lowest among species isolated (2.1%). The overall isolation rate of M. haemolytica, P. trehalosi and Pasteurella multocida was 15.7%, 11.5% and 2.9%, respectively. From 145 lung samples collected and cultured, Pasteurella was isolated successfully in 35 (24.1%) sheep. Out of 35 sheep lung lesion sample, the percentage recovery rate of M. haemolytica 17(11 .7%), P. trehalose 15(10.3%) and P. multocida 3(2.1) % respectively. On the basis of these results, M. haemolytica and P.trehalose were the most common cause of pasteurellosis in sheep at the study area. A total of 150 sheep sera were examined for serotype specific antibodies using indirect haemagglutination test for M. haemolytica, P. trehalose and P. multocida serotypes. Variation in prevalence among the different serotypes was observed (P<0.001). The IHA test revealed that serotype A1, A2, A7, T3, T10, a nd T15 were the dominant serotypes with 23.3%, 42.6%, 32, 51.3, 29.3 and 30% positive by IHA whereas serotypes P. multocida biotype A and T4 were the least positive with 14.6% and16% respectively. Generally, both bacterial and serological results of this s tudy revealed that the causal agents of pasteurellosis are prevalent in the area, and serotypes A1, A2, A7, T3, T10 and T15 were dominant over the other serotypes

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

scholarly journals Estimating Clinically Relevant Cut-Off Values for a High-Throughput Quantitative Real-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle

2021 ◽  
Vol 8 ◽  
Author(s):  
Alicia F. Klompmaker ◽  
Maria Brydensholt ◽  
Anne Marie Michelsen ◽  
Matthew J. Denwood ◽  
Carsten T. Kirkeby ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Pasteurella Multocida ◽  
Age Groups ◽  
Therapeutic Interventions ◽  
Respiratory Pathogens ◽  
Logistic Regression Models ◽  
Histophilus Somni ◽  
Nasal Swabs

Bovine respiratory disease (BRD) results from interactions between pathogens, environmental stressors, and host factors. Obtaining a diagnosis of the causal pathogens is challenging but the use of high-throughput real-time PCR (rtPCR) may help target preventive and therapeutic interventions. The aim of this study was to improve the interpretation of rtPCR results by analysing their associations with clinical observations. The objective was to develop and illustrate a field-data driven statistical method to guide the selection of relevant quantification cycle cut-off values for pathogens associated with BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based on nasal swabs from calves. We used data from 36 herds enrolled in a Danish field study where 340 calves within pre-determined age-groups were subject to clinical examination and nasal swabs up to four times. The samples were analysed with the rtPCR system. Each of the 1,025 observation units were classified as sick with BRD or healthy, based on clinical scores. The optimal rtPCR results to predict BRD were investigated for Pasteurella multocida, Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica, and Trueperella pyogenes by interpreting scatterplots and results of mixed effects logistic regression models. The clinically relevant rtPCR cut-off suggested for P. multocida and M. bovis was ≤ 21.3. For H. somni it was ≤ 17.4, while no cut-off could be determined for M. haemolytica and T. pyogenes. The demonstrated approach can provide objective support in the choice of clinically relevant cut-offs. However, for robust performance of the regression model sufficient amounts of suitable data are required.

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

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