Аpplication of xenogeneic native bone collagen for bone repair in critical-sized rat calvarial defect model

Aim. To evaluate the efficacy of bone repair using xenogeneic native bone collagen (Bongraf COLLAGEN) as compared to other widely applied orthopaedic solutions (xenogeneic native bone mineral Geistlich Bio-Oss® and bone autograft).Materials and Methods. We employed a conventional critical-sized (8 mm) rat calvarial defect model (48 Wistar or Sprague-Dawley rats). The artificial defect was repaired using Bongraf COLLAGEN, Geistlich Bio-Oss® utilised as a comparator, bone calvarial autograft, or remained unfilled (n = 6 per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 3 per time point) with the subsequent examination (repair extent, volume, thickness and mineral density of the repaired tissue) by means of microcomputed tomography and hematoxylin and eosin staining.Results. Expectedly, highest volume, thickness and mineral density of the repaired tissue have been observed in defects filled with autografts. Bongraf COLLAGEN and Geistlich Bio-Oss® also demonstrated a comparable and significant repair capability, yet the former option demonstrated higher bone regeneration rate.Conclusion. Xenogeneic native bone collagen (Bongraf COLLAGEN) is comparable with xenogeneic native bone mineral (Geistlich Bio-Oss®).

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Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects

Materials ◽

10.3390/ma13153393 ◽

2020 ◽

Vol 13 (15) ◽

pp. 3393 ◽

Cited By ~ 1

Author(s):

Alexey Veremeev ◽

Roman Bolgarin ◽

Vladimir Nesterenko ◽

Alexander Andreev-Andrievskiy ◽

Anton Kutikhin

Keyword(s):

Bone Mineral ◽

Bone Repair ◽

Bone Matrix ◽

Bone Collagen ◽

Demineralised Bone Matrix ◽

Bovine Bone ◽

Mineral Density ◽

Artificial Defect ◽

Demineralised Bone ◽

Mineral Powder

Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies.

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Xenogeneic bone mineral is efficient for the repair of critical-sized rat calvarial defects

Fundamental and Clinical Medicine ◽

10.23946/2500-0764-2021-6-1-16-26 ◽

2021 ◽

Vol 6 (1) ◽

pp. 16-26

Author(s):

A. V. Veremeev ◽

R. N. Bolgarin ◽

V. G. Nesterenko ◽

A. A. Andreev-Andrievskiy

Keyword(s):

Bone Graft ◽

Bone Mineral ◽

Bone Repair ◽

3D Structure ◽

Autologous Bone Graft ◽

Bone Volume ◽

Calvarial Defect ◽

Sprague Dawley ◽

Mineral Density ◽

Autologous Bone

Aim. To evaluate the efficiency of bone repair on a critical-sized rat calvarial defect model using our original xenogeneic bone mineral, widely established Geistlich Bio-Oss®, and autologous bone graft.Materials and Methods. We created a critical-sized calvarial defect in Sprague-Dawley rats (n = 48) and then divided them into 4 groups (unfilled defect, autologous bone graft, Geistlich BioOss® and our original xenogeneic bone mineral, 12 rats per group). Rats were sacrificed upon 4 and 12 months (6 rats per time point) with the following excision of the implant and adjacent tissues. 3D structure, extent of mineralisation, and bone volume were measured by means of microcomputed tomography. Microanatomy of the explants and adjacent tissue was investigated by haematoxylin and eosin staining.Results. The highest and the lowest bone volume was expectedly detected when the defect was filled with the autologous bone graft or remained unfilled, respectively. Replacement of the defect by the original bone mineral entailed better regeneration as compared to Geistlich Bio-Oss. Bone mineral density, bone thickness and the extent of mineralisation did not differ significantly between the experimental groups and were close to the positive control values, indicating efficient bone repair.Conclusions. Original xenogeneic bone mineral promotes induction of bone regeneration as compared to Geistlich Bio-Oss®, a commercially available bone mineral widely used in the clinical practice.

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Osteogenic potential of cultured human periosteum-derived cells – A pilot study of human cell transplantation into a rat calvarial defect model

Journal of Cranio-Maxillofacial Surgery ◽

10.1016/j.jcms.2006.07.861 ◽

2006 ◽

Vol 34 (8) ◽

pp. 461-465 ◽

Cited By ~ 37

Author(s):

Yoshiro Sakata ◽

Takaaki Ueno ◽

Toshimasa Kagawa ◽

Miwa Kanou ◽

Takashi Fujii ◽

...

Keyword(s):

Pilot Study ◽

Cell Transplantation ◽

Human Cell ◽

Osteogenic Potential ◽

Calvarial Defect ◽

Defect Model ◽

Rat Calvarial Defect

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Photo-immobilization of bone morphogenetic protein-2 using azidophenyl gelatin on a collagen sheet enhances osteogenesis in a rat calvarial defect model

Journal of Industrial and Engineering Chemistry ◽

10.1016/j.jiec.2016.06.021 ◽

2016 ◽

Vol 40 ◽

pp. 177-184 ◽

Cited By ~ 5

Author(s):

Dae Woong Ham ◽

Eui Chan Jang ◽

Tae Il Son ◽

Tae Jin Lee ◽

Kwang-Sup Song

Keyword(s):

Bone Morphogenetic Protein ◽

Bone Morphogenetic Protein 2 ◽

Calvarial Defect ◽

Defect Model ◽

Morphogenetic Protein ◽

Rat Calvarial Defect

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Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

Journal of Materials Chemistry B ◽

10.1039/c8tb03361g ◽

2019 ◽

Vol 7 (20) ◽

pp. 3250-3259 ◽

Cited By ~ 2

Author(s):

Yali Miao ◽

Yunhua Chen ◽

Xiao Liu ◽

Jingjing Diao ◽

Naru Zhao ◽

...

Keyword(s):

Bone Regeneration ◽

Tricalcium Phosphate ◽

Calvarial Defect ◽

Defect Model ◽

Beta Tricalcium Phosphate ◽

3D Printed ◽

Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

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Bone quality associated with daily intake of coffee: a biochemical, radiographic and histometric study

Brazilian Dental Journal ◽

10.1590/s0103-64402010000300004 ◽

2010 ◽

Vol 21 (3) ◽

pp. 199-204 ◽

Cited By ~ 16

Author(s):

Suzie Aparecida Lacerda ◽

Renata Inahara Matuoka ◽

Rander Moreira Macedo ◽

Sergio Olavo Petenusci ◽

Alessandra Aparecida Campos ◽

...

Keyword(s):

Bone Mineral Density ◽

Bone Quality ◽

Bone Mineral ◽

Bone Repair ◽

Daily Intake ◽

Male Rats ◽

Lower Amount ◽

Caffeine Intake ◽

Mineral Density ◽

Coffee Intake

Caffeine induces loss of calcium and influences the normal development of bone. This study investigated the effects of coffee on bone metabolism in rats by biochemical measurement of calcium, bone densitometry and histometry. Male rats, born of female treated daily with coffee and with coffee intake since born, were anesthetized, subjected to extraction of the upper right incisor, and sacrificed 7, 21 and 42 days after surgery. Blood and urine samples were taken, and their maxilla radiographed and processed to obtain 5-µm-thick semi-serial sections stained with hematoxylin and eosin. The volume and bone quality were estimated using an image-analysis software. The results showed significantly greater amount of calcium in the plasma (9.40 ± 1.73 versus 9.80 ± 2.05 mg%) and urine (1.00 ± 0.50 versus 1.25 ± 0.70 mg/24 h) and significantly less amount in bone (90.0 ± 1.94 versus 86.0 ± 2.12 mg/mg bone), reduced bone mineral density (1.05 ± 0.11 versus 0.65 ± 0.15 mmAL), and lower amount of bone (76.19 ± 1.6 versus 53.41 ± 2.1 %) (ANOVA; p≤0.01) in animals treated with coffee sacrificed after 42 days. It may be concluded that coffee/caffeine intake caused serious adverse effects on calcium metabolism in rats, including increased levels of calcium in the urine and plasma, decreased bone mineral density and lower volume of bone, thus delaying the bone repair process.

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