scholarly journals STOKES-PARAMETRIC AUTOFLUORESCENCE MICROSCOPIC IMAGES OF OPTICALLY ANISOTROPIC HISTOLOGICAL SECTIONS OF THE PANCREATIC TISSUES OF RATS AT THE EARLY STAGES OF EXPERIMENTAL DIABETES

2015 ◽  
Vol 14 (4) ◽  
pp. 99-103
Author(s):  
T. M. Boichuk ◽  
O. H. Ushenko ◽  
M. I. Hrytsiuk
Keyword(s):  
Experimental Diabetes ◽  
Microscopic Images ◽  
Histological Sections ◽  
Early Stages

MORPHOFUNCTIONAL CHANGES IN THE LIVER OF 24-MONTH-OLD RATS IN THE EARLY STAGES OF EXPERIMENTAL DIABETES MELLITUS DEVELOPMENT

2018 ◽  
Vol 14 (65) ◽  
pp. 152
Author(s):  
O. Ya. Zhurakivska ◽  
Yu. V. Bodnarchuk ◽  
V. M. Pertsovych ◽  
H. B. Kulynych ◽  
V. A. Мiesoiedova
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Experimental Diabetes Mellitus ◽  
Early Stages ◽  
Old Rats

Upregulation of PKC genes and isozymes in cardiovascular tissues during early stages of experimental diabetes

2003 ◽  
Vol 12 (2) ◽  
pp. 139-146 ◽  
Author(s):  
Mingzhang Guo ◽  
Mack H. Wu ◽  
Ferenc Korompai ◽  
Sarah Y. Yuan
Keyword(s):  
Protein Expression ◽  
Cardiovascular System ◽  
Time Course ◽  
Experimental Diabetes ◽  
Pathological Condition ◽  
Expression Profiles ◽  
Diabetic Patients ◽  
Mrna Levels ◽  
Early Stages ◽  
Protein Expression Profiles

The protein kinase C (PKC) pathway has recently been recognized as an important mechanism in the development of diabetic complications including cardiomyopathy and angiopathy. Although an increase in PKC kinase activity has been detected in the cardiovascular system of diabetic patients and animals, it is unclear whether the same pathological condition alters PKC at the transcriptional and translational levels. In this study we assessed quantitatively the mRNA and protein expression profiles of PKC isozymes in the heart and vascular tissues from streptozotocin-induced diabetic pigs. Partial regions of the porcine PKCα, β1, and β2 mRNAs were sequenced, and real-time RT-PCR assays were developed for PKC mRNA quantification. The results showed a significant increase in the mRNA levels of PKCα, β1, and β2 in the heart at 4–8 wk of diabetes. In concomitance, the PKCβ1 and β2 genes, but not the PKCα gene, were upregulated in the diabetic aorta. Correspondingly, there was a significant increase in the protein expression of PKCα and β2 in the heart and PKCβ2 in the aorta with a time course correlated to that of mRNA expression. In summary, PKCβ2 was significantly upregulated in the heart and aorta at both the transcriptional and translational levels during early stages of experimental diabetes, suggesting that PKCβ2 may be a prominent target of diabetic injury in the cardiovascular system.

scholarly journals Differential diagnostics of the presence of damage by the method of digital polarization mapping of microsocopy images of hystological sections of human organs

2021 ◽  
pp. 70-78
Author(s):  
Oleksandra Litvinenko ◽  
Oleh Vanchulyak ◽  
Irina Soltys ◽  
Olga Mikhailova ◽  
Artem Motrich
Keyword(s):  
Lung Tissue ◽  
Experimental Testing ◽  
Biological Tissues ◽  
Differential Diagnostics ◽  
Time Interval ◽  
Diagnostic Efficiency ◽  
Internal Organs ◽  
Microscopic Images ◽  
Histological Sections ◽  
Ellipticity Of Polarization

The article presents the results of experimental testing of methods of azimuthal-invariant polarization mapping of microscopic images of samples of histological sections of the myocardium and lung tissue; time monitoring of changes in the magnitude of statistical moments of the 1-4th orders, characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of myocardial and lung tissue samples with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping azimuth maps and polarization ellipticity of microscopic images. Aim of the work. Development of a digital histology technique for samples of human internal organs. Materials and methods. The object of the study was the histology of samples of human internal organs (myocardium, lung tissue) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples from those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the method of digital polarization mapping of microscopic images of histological sections of biological tissues of human internal organs. Results. Revealed the following ranges ramp variation quantity statistics polarizing digital histology and accuracy of the limitation of damage: a. Arts azimuth polarization microscopic image of a magnification ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 55-60 min), maps of ellipticity of polarization of microscopic images with a magnification of ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 65-75 min). Conclusions. Scenarios of changes in the statistical structure of maps of azimuth and ellipticity of polarization of microscopic images of histological sections of human internal organs have been determined - with an increase in the duration of damage, the value of the mean and variance decreases, the asymmetry and kurtosis increase.

scholarly journals Histological Changes of the Mandibular Condyle in the Human Fetus at Early Stages of Gestation

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Wilfredo Molina
Keyword(s):  
Bone Formation ◽  
Human Fetus ◽  
Mandibular Condyle ◽  
Endochondral Bone Formation ◽  
Endochondral Bone ◽  
Histological Changes ◽  
Histological Sections ◽  
Early Stages ◽  
Mesenchymal Tissue ◽  
Periodic Reaction

Histochemical studies on the mandibular condyle of the human fetus at gestational ages 12, 14, and 16 weeks were performed. Methods. Histological sections were stained with Schiff’s periodic reaction for glicoproteins, hematoxiline eosine detects mesenchymal tissue and trichhromic stain for collagen. The ANOVA one-way test was used to evaluate the differences during stained zones in the three fetus groups. Results. The percentage of glycoproteins and mesenchymal tissue was denser at 12 weeks. This percentage decreases at 14 weeks and is less at 16 weeks. An increase in the amount of collagen in the studied weeks was observed. The percentages of glycoproteins, mesenchymal tissue, and collagen were significantly different; f = 4373, 9624.8, and 3674, P<0.0001 for the three studied groups. Conclusion. The endochondral bone formation of the mandibular condyle includes modifications of the quantities of glycoproteins, mesenchymal tissue, and collagen.

scholarly journals Laser polarization-variable autofluorescence of the network of optically anisotropic biological tissues: Diagnostics and differentiation of early stages of cancer ofcervix uteri

2014 ◽  
Vol 07 (03) ◽  
pp. 1450024 ◽  
Author(s):  
Yu. A. Ushenko ◽  
M. I. Sidor ◽  
N. Pashkovskaia ◽  
G. D. Koval ◽  
Yu. F. Marchuk ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Cervix Uteri ◽  
Linear Birefringence ◽  
Laser Polarization ◽  
Histological Sections ◽  
Early Stages ◽  
Operational Characteristics ◽  
Carcinoma Of Cervix ◽  
Endometrium Biopsy ◽  
Sensitivity Specificity

This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears) in the task of diagnostics and differentiation of early stages of cancer: Dysplasia — cervical microinvasive carcinoma of cervix uteri. The analytical conditions of polarization-optimal probing of biological layers were determined basing on the model of linear birefringence and dichroism of birefringent (fibrillar, porphyrin) networks. The technique of polarization-variable laser autofluorescence was developed and experimentally tested. The objective criteria (statistical moments) of differentiation of histological sections autofluorescent images of endometrium biopsy and cytological smears of it mucous coat were defined. The operational characteristics (sensitivity, specificity, accuracy) of this technique were determined concerning the positions of probative medicine, and clinical efficiency.

scholarly journals ENTERAL INSUFFICIENCY COURSE SEVERITY DEGREES DIAGNOSTICS IN PERITONITIS BY SMALL INTESTINAL MICROSCOPIC IMAGES PHASE MAPING

2021 ◽  
pp. 25-30
Author(s):  
V. P. Polyovyy ◽  
R. I. Sydorchuk ◽  
S. P. Polyova ◽  
A. S. Palianytsia ◽  
I. G. Chepega
Keyword(s):  
Small Intestine ◽  
Phase Measurement ◽  
Measurement Data ◽  
Statistical Parameters ◽  
Logical Scheme ◽  
Microscopic Images ◽  
Laboratory Rats ◽  
Histological Sections ◽  
Insufficiency Syndrome ◽  
Polarization Phase

Summary. The aim is to determine the efficiency of diagnostics of the enteric insufficiency syndrome severity in peritonitis by phase mapping of microscopic images of histological sections of the laboratory rats’ small intestine. Material and methods. Seventy-eight white nonlinear rats of both sexes were selected, weighing from 180 to 220 g, underwent simulated mild, moderate and severe experimental peritonitis according to the standard method. Samples of optically thin histological sections of the small intestine of rats with different severity of the course of peritonitis were used as the research object. Results. As a result of the study, a structural and logical scheme for studying the effectiveness of differential diagnosis of the severity of peritonitis by means of statistical analysis of the structure of the distribution of phases of microscopic images of biological preparations of rats was developed, which helped to determine the relationships between the distribution of statistical parameters of the 1st–4th orders, which characterize phase maps depending on the severity of the peritonitis course. Conclusion. The revealed changes in the statistical structure of phase maps fully correlate with the measurement data for the polarization phase manifestations of the properties of the polycrystalline component of histological sections, both in the case of the phase measurement of polycrystalline blood films and the wall of the small intestine.

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