scholarly journals Cutaneous hemangiosarcoma in a buff-throated saltator (Saltator maximus) with lung metastasis

2021 ◽  
Vol 14 (2) ◽  
pp. 107-110
Author(s):  
José Duarte ◽  
◽  
Francisca Barbosa ◽  
Rubia Sampaio ◽  
Rafael Oliveira ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Factor Viii ◽  
Blood Vessels ◽  
Lung Metastasis ◽  
Surgical Procedure ◽  
Histological Evaluation ◽  
Malignant Neoplasms ◽  
Factor Viii Related Antigen ◽  
Nodular Mass ◽  
Veterinary Hospital

Hemangiosarcomas are malignant neoplasms arising from endothelial cells of blood vessels. A three-year-old male buff-throated saltator (Saltator maximus) was referred to the Veterinary Hospital of the Universidade Federal da Paraíba (HV-UFPB) due to a recurrent tan nodular mass in the pericloacal region. Surgery for excision of the mass was attempted but the bird died during the surgical procedure. The nodule and the carcass were sent for gross and histological evaluation. Histopathology revealed large blood-filled vascular spaces lined by pleomorphic endothelial cells, which were also observed in the lungs. These findings suggested the diagnosis of hemangiosarcoma that was confirmed by immunohistochemistry against factor VIII-related antigen.

scholarly journals Localization of factor-VIII-related antigen in human vascular subendothelium

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 752-756
Author(s):  
JH Rand ◽  
II Sussman ◽  
RE Gordon ◽  
SV Chu ◽  
V Solomon
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Blood Vessel ◽  
Factor Viii ◽  
Platelet Adhesion ◽  
Vascular Endothelial Cells ◽  
Staining Technique ◽  
Immunofluorescent Staining ◽  
Vascular Endothelial ◽  
Factor Viii Related Antigen

Factor-VIII-related antigen has previously been shown to be synthesized by vascular endothelial cells. Using both an immunofluorescent staining technique and electron microscopy, we have demonstrated the presence of factor-VIII-related antigen in human vascular subendothelium. This finding may have implications in the mechanism of platelet adhesion to deendothelialized blood vessel surfaces.

scholarly journals Electron microscopic localization of factor-VIII-related antigen in adult human blood vessels

Blood ◽  
1982 ◽  
Vol 60 (3) ◽  
pp. 627-634
Author(s):  
JH Rand ◽  
RE Gordon ◽  
II Sussman ◽  
SV Chu ◽  
V Solomon
Keyword(s):  
Factor Viii ◽  
Blood Vessels ◽  
Human Blood ◽  
Morphological Evidence ◽  
Electron Microscopic ◽  
Factor Viii Related Antigen ◽  
Adult Human ◽  
Adhesion Of Platelets ◽  
Elastic Lamina ◽  
Electron Microscopic Localization

We have localized factor-VIII-related antigen, using immunofluorescence and electron microscopy, in adult human blood vessels. In addition to its presence in endothelial cells, the antigen was localized within subendothelium and the layers of elastic lamina closest to the lumen. Also, we provide the first morphological evidence that factor-VIII- related antigen is associated with collagen fibrils within the vessel wall. These studies suggest that this subendothelial factor-VIII- related antigen may play a role in the adhesion of platelets to subendothelial components following endothelial injury.

scholarly journals Localization of factor-VIII-related antigen in human vascular subendothelium

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 752-756 ◽  
Author(s):  
JH Rand ◽  
II Sussman ◽  
RE Gordon ◽  
SV Chu ◽  
V Solomon
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Blood Vessel ◽  
Factor Viii ◽  
Platelet Adhesion ◽  
Vascular Endothelial Cells ◽  
Staining Technique ◽  
Immunofluorescent Staining ◽  
Vascular Endothelial ◽  
Factor Viii Related Antigen

Abstract Factor-VIII-related antigen has previously been shown to be synthesized by vascular endothelial cells. Using both an immunofluorescent staining technique and electron microscopy, we have demonstrated the presence of factor-VIII-related antigen in human vascular subendothelium. This finding may have implications in the mechanism of platelet adhesion to deendothelialized blood vessel surfaces.

scholarly journals Ultrastructural localization of factor VIII-related antigen in cultured human brain microvessel endothelial cells.

1992 ◽  
Vol 40 (5) ◽  
pp. 689-696 ◽  
Author(s):  
K Dorovini-Zis ◽  
H K Huynh
Keyword(s):  
Endothelial Cells ◽  
Human Brain ◽  
Factor Viii ◽  
Ionophore A23187 ◽  
Specific Storage ◽  
Immunogold Staining ◽  
Factor Viii Related Antigen ◽  
Cytoplasmic Vesicles ◽  
Microvessel Endothelial Cells ◽  
Brain Microvessel

Immunogold staining of primary cultures of human brain microvessel endothelial cells demonstrated the presence of Factor VIII-related antigen within cytoplasmic vesicles in close association with the rough endoplasmic reticulum and Golgi apparatus. Immunoperoxidase staining, at the light microscopic level, revealed a similar granular, perinuclear staining. The morphology and location of these vesicular profiles indicate that they are part of the trans-Golgi region where terminal processing and short-term storage of Factor VIII-related antigen takes place. Weibel-Palade bodies, specific storage organelles for von Willebrand factor in large vessel endothelium, were not observed in cerebral microvessel endothelium. The release of Factor VIII-related antigen from the cytoplasmic vesicles was influenced by some of the factors known to stimulate or inhibit the regulated pathway of secretion from Weibel-Palade bodies. Thus, stimulation of endothelial cells with calcium ionophore A23187 resulted in almost complete loss of staining, while addition of EGTA to the culture medium led to slight increase of intracellular pools of Factor VIII-related antigen. Pre-incubation of monolayers with interferon-gamma was associated with significant increase in the number of labeled vesicles, suggesting an additional role of this cytokine in the localized immune reaction within the central nervous system.

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