scholarly journals Effect of growth regulators on rooting of Three Citrus rootstock propagated in vitro

2012 ◽  
Vol 6 (2) ◽  
pp. 62-69
Author(s):  
Z.A. Hussain ◽  
W. Abd-Alazez ◽  
A. Rdha ◽  
F. N. Hassan
Keyword(s):  
Growth Regulators ◽  
Shoot Length ◽  
Green House ◽  
Carrizo Citrange ◽  
Citrus Rootstock ◽  
Diluted Solution ◽  
Concentrated Solution ◽  
Troyer Citrange

oft cuttings 15cm of three citrus rootstocks (Carrizo citrange, Troyer citrange, Swingle citrumello) which propagated in vitro and transplanting in Green house, and rooted in diluted solution at (0.0, 10NAA,10 IBA, 10 NOA, 10NAA+2IBA, 10 IBA+2NAA, 10NOA+2IBA) mg/L and Concentrated solution at (0.0, 1000 IAA,1000 IBA,1000 NAA,1000 NOA mg/L and Serdix, Serdix with sol.). Data of Rooting percentage, number of roots per plant after (40,50) days and their root lengths were investigated. Results showed that diluted solution significant effect on all character after (40,50) days, Based on diluted solution (10NAA+2 1BA) mg/L showed the highest rooting percentage, root per shoot, length of shoot reached (60%, 1.47 root/culting, 3.69cm ), respectively after 50 day While, there was no significant effected of concentrated solution in Rooting percentage after 40 days, 1000mg/L IAA gave highest rooting percentage reached 66.67% and highest mean in roots per shoot and length (1.53 root/culting , 2.77cm) respectively , after 50 days. There was no significant effect of stocks on Rooting percentage by using diluted and concentrated solution While, Troyer citrange showed highest mean in number of root reached 1.2 root/ culting, Carrizo citrange gave highest mean in length of roots reached 2.33 cm by using concentrated solution.

In vitro propagation of Troyer Citrange : A horticulturally important citrus rootstock

Green Farming ◽  
2020 ◽  
Vol 11 (2-3) ◽  
pp. 130
Author(s):  
VIJAYAKUMARI N. ◽  
P. GHOSH ◽  
Y.B. LAHANE ◽  
K.P. FISKE
Keyword(s):  
In Vitro Propagation ◽  
Citrus Rootstock ◽  
Troyer Citrange

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals In vitro Propagation of Bacopa monnieri (L.)

2020 ◽  
pp. 32-39
Author(s):  
Poornima Raj ◽  
J. Anbumalarmathi ◽  
S. Aruna Sharmili
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Bacopa Monnieri ◽  
Shoot Length ◽  
Ms Medium ◽  
Root Initiation ◽  
Shoot Induction ◽  
Propagation Technique

An experiment was conducted for standardization of in vitro propagation technique of Bacopa monnieri (L.), a medicinal herb of India. Healthy leaf segments of the herb were used as explants with basic Murashige and Skoog (MS) medium containing various combinations of different growth regulators for callus, shoot and root initiation. The best callus induction percentage (95.47%) was observed on MS + 0.5 mg/L NAA and 2.0 mg/L BAP (T3). The maximum number of shoots (8), shoot length (9.30 cm) and shoot induction percentage (90.48%) was achieved on MS + 3.0 mg/L BAP and 1.0 mg/L Kn (ST4). The maximum number of roots (8) and root length (7) was observed on MS + 1.5 mg/L IAA (RT5). The rooted micro shoots were successfully hardened and acclimatized in green house and subsequently established in soil with survival rate of 90%.

scholarly journals In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

2020 ◽  
Vol 66 (4) ◽  
pp. 25-31
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Leaf Area ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation ◽  
Number Of Leaves ◽  
In Vitro Clonal Propagation

Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.

scholarly journals In vitro establishment of pepper-rosmarin nodal segments

2008 ◽  
Vol 26 (2) ◽  
pp. 255-258 ◽  
Author(s):  
Arie F Blank ◽  
Andréa S da Costa ◽  
Maria de Fátima Arrigoni-Blank ◽  
Aline B de Mendonça ◽  
Ana da S Ledo
Keyword(s):  
Essential Oil ◽  
Medicinal Plant ◽  
Growth Regulators ◽  
Culture Medium ◽  
Antimicrobial Properties ◽  
Shoot Length ◽  
Nodal Segments ◽  
Lippia Sidoides ◽  
In Vitro Establishment

Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA (0.0; 0.5; 1.0; 1.5; and 2.0 mg L-1) in assay 4; and five concentrations of GA3 (0.0; 0.1; 0.5; 1.0; and 1.5 mg L-1) in assay 5. The use of BAP, IBA, NAA and KIN did not influence the number of shoots. The addition of 0.1 mg L-1 of NAA at the culture medium resulted in larger shoot length per explant, while the concentration of 1.0 mg L-1 of kinetin promoted larger shoot length in general. GA3 did not affect the elongation of pepper-rosmarin shoots when cultivated in vitro.

scholarly journals Increasing In Vitro Shoot Elongation and Proliferation of ‘G.30’ and ‘G.41’ Apple by Chilling Explants and Plant Growth Regulators

HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 899-904 ◽  
Author(s):  
Fang Geng ◽  
Renae Moran ◽  
Michael Day ◽  
William Halteman ◽  
Donglin Zhang
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Growth ◽  
Shoot Elongation ◽  
Shoot Length ◽  
Optimum Concentration ◽  
Shoot Number ◽  
Collection Date

These studies were conducted to determine the most effective methods for increasing shoot elongation during the initial proliferation stage of micropropagation in two dwarfing apple, Malus ×domestica (Borkh.), rootstock cultivars. Several experiments were conducted to compare explant collection date, exposure to chilling (5 ± 1 °C) temperatures, and varying concentrations of plant growth regulators in Murashige and Skoog (MS) media. Microshoot growth of ‘Geneva 41’ (‘G.41’) was very low and unaffected by chilling duration from 0 to 8 weeks or by gibberellic acid (GA3) concentration from 0 to 1.0 mg·L−1, but was improved by an additional subculture which increased shoot length from 1 to 15 mm. In ‘Geneva 30’ (‘G.30’), shoot elongation was most affected by date, chilling explants, and by optimizing cytokinin concentration and type. Explant collection date in April increased shoot growth compared with August or November. Microshoot growth of ‘G.30’ was increased by chilling nodal explants for 4 and 6 weeks when explants were collected in August and November, but not in April. Eight weeks chilling was detrimental for explants collected in April, and generally had little or no effect with August and November. The cytokinin 6-benzylaminopurine (BA) increased shoot number to a greater extent than thidiazuron (TDZ) or zeatin (ZT), and was also more effective for increasing shoot elongation with concentrations of 0 to 2.0 mg·L−1. In ‘G.30’, GA3 increased shoot growth at the optimum concentration of BA, but not with lower concentrations. ‘G.30’ microshoots were fewer and shorter with 24-epi-brassinolide (EBR) at concentrations of 0.1 and 1.0 mg·L−1. Chemical names: N-phenyl-N’-(1,2,3-thiadiazol-5-yl)urea (TDZ), 6-(4-hydroxy-3-methylbut-2-enylamino)purine (ZT).

scholarly journals Carrizo citrange Plants Do Not Require the Presence of Roots to Modulate the Response to Osmotic Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Rosa M. Pérez-Clemente ◽  
Almudena Montoliu ◽  
Sara I. Zandalinas ◽  
Carlos de Ollas ◽  
Aurelio Gómez-Cadenas
Keyword(s):  
Osmotic Stress ◽  
Antioxidant Activities ◽  
Culture Media ◽  
Stress Conditions ◽  
Plant Performance ◽  
Plant Responses ◽  
Adverse Condition ◽  
Carrizo Citrange ◽  
Citrus Rootstock

The study of the effects of a specific stress condition on the performance of plants grown under field conditions is difficult due to interactions among multiple abiotic and biotic factors affecting the system.In vitrotissue-culture-based techniques allow the study of each adverse condition independently and also make possible to investigate the performance of genotypes of interest under stress conditions avoiding the effect of the root. In this paper, the response of Carrizo citrange, a commercial citrus rootstock, to osmotic stress was evaluated by culturingin vitrointact plants and micropropagated shoots. The osmotic stress was generated by adding two different concentrations of polyethyleneglycol to the culture media. Different parameters such as plant performance, organ length, antioxidant activities, and endogenous contents of proline, malondialdehyde, and hormones were determined. Differently to that observed under high salinity, when subjected to osmotic stress conditions, Carrizo citrange showed increased endogenous levels of MDA, proline, and ABA. These results evidence that the mechanisms of response of Carrizo citrange to saline or osmotic stress are different. The presence of roots was not necessary to activate any of the plant responses which indicates that the organs involved in the stress perception and signaling depends on the type of adverse condition to which plants are subjected.

scholarly journals Multiplicação in vitro de Caesalpinia pyramidalis (Leguminosae)

2013 ◽  
Vol 13 ◽  
Author(s):  
Tecla Dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Bárbara Paula dos Santos Borges ◽  
Bruno Freitas Matos Alvim ◽  
José Raniere Ferreira De Santana
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Dry Mass ◽  
Shoot Length ◽  
Nodal Segments ◽  
In Vitro Multiplication ◽  
Different Types

Caesalpinia pyramidalis is a species endemic to the Caatinga and known popularly as catingueira, which is widely used by local people, mainly for its timber and medicinal and fodder properties. This study investigated the effects of different types and concentrations of plant growth regulators on the in vitro multiplication of C. pyramidalis. In the first experiment, nodal segments were inoculated in media containing different combinations (0.0–8.0 µM) of BAP and NAA. In the second experiment, nodal segments wereinoculated in media containing different types (KIN, BAP and TDZ) and concentrations (0.0–16μM) of cytokinins. We used a WPM medium supplemented with 87.64 mM sucrose and solidified with 7.0 g L-1 agar. After 45 days, the highest number of shoots, leaf number, shoot length and dry mass of shoots were obtained when nodal segments were inoculated into a culture medium without plant growth regulators.

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