scholarly journals Occurrence of Anaplasmosis (Anaplasma Marginale) in cattle in Sulaimani province, Kurdistan region of Iraq

passer ◽  
2019 ◽  
Vol 3 (1) ◽  
pp. 180-186
Author(s):  
Shakhawan Latif Mahmmod ◽  
Rebwar Bahir Ahmed ◽  
Nawroz Akram Kakarash ◽  
Ihsan K Zangana ◽  
Mohammed Omar Baba Sheikh
Keyword(s):  
Microscopic Examination ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
P Value ◽  
Kurdistan Region ◽  
Pcr Assay ◽  
Msp4 Gene ◽  
Significant Difference ◽  
Major Surface ◽  
First Time

The goal of this study was to determine both the incidence of anaplasmosis (Anaplasma Marginale) and phylogenetic relationship between A. marginale isolates from cattle in Sulaimani province, Kurdistan Region- Iraq during (March 10th to April 10th 2021) and those from other Anaplasma spp. A total of two isolates were tested for the major surface protein (msp4) gene for this purpose. Eighty blood samples of cattle (51 males and 29 females) were examined using both microscopic examination and PCR tests. Overall results were 23/80 (28.7 5%) and 8/80 (10 %) using microscopic examination and PCR assay, respectively. Age and sex were not significant factors in the appearance of infection, since no statistically significant difference in infection rate has been observed among sex and age group of cattle (P value >0.05). The results also revealed that the accuracies of traditional method and PCR assays in the diagnosis of the disease were 81 %, and 100 respectively. There was moderate correlation (0.43) between both techniques by the Kappa (k) test. However, The PCR technique recorded the highest sensitivity (100%) and specify (100%) for A. marginale detection. In conclusion, by the findings of the present study, it has been confirmed for the first time that A. marginale is the causative agent of anaplasmosis of cattle in the study areas and the best technique for the detection of either acute or chronic cases in cattle was the PCR assay.

scholarly journals Anaplasma marginale Major Surface Protein 2 CD4+-T-Cell Epitopes Are Evenly Distributed in Conserved and Hypervariable Regions (HVR), Whereas Linear B-Cell Epitopes Are Predominantly Located in the HVR

2004 ◽  
Vol 72 (12) ◽  
pp. 7360-7366 ◽  
Author(s):  
Jeffrey R. Abbott ◽  
Guy H. Palmer ◽  
Chris J. Howard ◽  
Jayne C. Hope ◽  
Wendy C. Brown
Keyword(s):  
T Cell ◽  
B Cell ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
T Cell Epitopes ◽  
B Cell Epitopes ◽  
Major Surface Protein ◽  
Hypervariable Regions ◽  
Major Surface ◽  
Linear B

ABSTRACT Organisms in the genus Anaplasma express an immunodominant major surface protein 2 (MSP2), composed of a central hypervariable region (HVR) flanked by highly conserved regions. Throughout Anaplasma marginale infection, recombination results in the sequential appearance of novel MSP2 variants and subsequent control of rickettsemia by the immune response, leading to persistent infection. To determine whether immune evasion and selection for variant organisms is associated with a predominant response against HVR epitopes, T-cell and linear B-cell epitopes were localized by measuring peripheral blood gamma interferon-secreting cells, proliferation, and antibody binding to 27 overlapping peptides spanning MSP2 in 16 cattle. Similar numbers of MSP2-specific CD4+ T-cell epitopes eliciting responses of similar magnitude were found in conserved and hypervariable regions. T-cell epitope clusters recognized by the majority of animals were identified in the HVR (amino acids [aa] 171 to 229) and conserved regions (aa 101 to 170 and 272 to 361). In contrast, linear B-cell epitopes were concentrated in the HVR, residing within hydrophilic sequences. The pattern of recognition of epitope clusters by T cells and of HVR epitopes by B cells is consistent with the influence of protein structure on epitope recognition.

scholarly journals Microscopic And Molecular Identification of Anaplasma Ovis in Small Ruminants in Duhok Province in Kurdistan Region of Iraq

2020 ◽  
Vol 23 (2) ◽  
pp. 228-235
Author(s):  
Adnan Ahmed ◽  
Jassim M Abdo
Keyword(s):  
Microscopic Examination ◽  
Prevalence Rate ◽  
Small Ruminants ◽  
Surface Protein ◽  
Positive Sample ◽  
Pcr Analysis ◽  
Anaplasma Ovis ◽  
Blood Samples ◽  
Major Surface Protein ◽  
Major Surface

In last ten years, there has been a developing enthusiasm for microscopic organisms from the genus Anaplasma, particularly the species A. ovis. It is associated with the pathogenic action of these microscopic organisms in livestock. Anaplasma ovis is a tick-borne obligate intracellular rickettsial bacterium that causes anaplasmosis in domestic and wild small ruminants. The samples of the present study were collected from small ruminants from inside seven distinct regions (Akre, Simele, Zummar, Feshchapoor, Deraboon, Bajed Kandal,Karoda)of Duhok province, 389 (goats 75 and sheep 314) during the period of April and May 2018, blood sample were taken and thin smear was formed, after Giemsa’s staining the slide is observed under microscope. In this study used Giemsa stain for microscopic examination out of 389 animals 250 were found positive for Anaplasma ovis infection with a prevalence rate of 64.26 % and 139 of them were negative with a prevalence rate of 35.73 %. According to the species of animals, the highest prevalence of A. ovis infection in animals by using microscopic examination was 67.83 %, 213 positive sample from total 314 blood samples from sheep and lowest prevalence was 49.33 %, 37 positive sample from total 75 blood samples from goats. PCR analysis of 100 blood samples obtained from total 250 positive blood samples after DNA extraction and measure of concentration and purity we used 2 primers that target major surface protein 4 (MSP4) in A. ovis genomic DNA. The results of PCR test with major surface protein 4 primer was 83 samples positive from total 100 samples, According to the species of animals, the highest prevalence of A. ovis was 83.7 %, 72 positive sample from total 86 blood samples from sheep and lowest prevalence was 78.5 %, 11 positive sample from total 14 blood samples from goats.

scholarly journals Comparison between indirect enzyme-linked immunosorbent assays for Anaplasma marginale antibodies with recombinant major surface protein 5 and initial body antigens

2006 ◽  
Vol 101 (5) ◽  
pp. 511-516 ◽  
Author(s):  
Virgínia MG Silva ◽  
Flábio R Araújo ◽  
Claudio R Madruga ◽  
Cleber O Soares ◽  
Raul H Kessler ◽  
...  
Keyword(s):  
Surface Protein ◽  
Anaplasma Marginale ◽  
Major Surface Protein ◽  
Immunosorbent Assays ◽  
Major Surface

Phylogeography of New World isolates of Anaplasma marginale based on major surface protein sequences

2002 ◽  
Vol 88 (3) ◽  
pp. 275-285 ◽  
Author(s):  
José de la Fuente ◽  
Ronald A Van Den Bussche ◽  
Jose C Garcia-Garcia ◽  
Sergio D Rodrı́guez ◽  
Miguel A Garcı́a ◽  
...  
Keyword(s):  
New World ◽  
Protein Sequences ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Major Surface Protein ◽  
Major Surface

scholarly journals Infection of Tick Cells and Bovine Erythrocytes with One Genotype of the Intracellular Ehrlichia Anaplasma marginale Excludes Infection with Other Genotypes

2002 ◽  
Vol 9 (3) ◽  
pp. 658-668 ◽  
Author(s):  
José de la Fuente ◽  
Jose C. Garcia-Garcia ◽  
Edmour F. Blouin ◽  
Jeremiah T. Saliki ◽  
Katherine M. Kocan
Keyword(s):  
United States ◽  
Tandem Repeats ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
The United States ◽  
The Other ◽  
Tick Cell ◽  
Anaplasma Ovis ◽  
Major Surface ◽  
Bovine Erythrocytes

ABSTRACT Anaplasma marginale, a tick-borne rickettsial pathogen of cattle, is endemic in several areas of the United States. Many geographic isolates of A. marginale that occur in the United States are characterized by the major surface protein 1a, which varies in sequence and molecular weight due to different numbers of tandem repeats of 28 or 29 amino acids. Recent studies (G. H. Palmer, F. R. Rurangirwa, and T. F. McElwain, J. Clin. Microbiol. 39:631-635, 2001) of an A. marginale-infected herd of cattle in an area of endemicity demonstrated that multiple msp1α genotypes were present but that only one genotype was found per individual bovine. These findings suggested that infection of cattle with other genotypes was excluded. The present study was undertaken to confirm the phenomenon of infection exclusion of A. marginale genotypes in infected bovine erythrocytes and cultured tick cells. Two tick-transmissible isolates of A. marginale, one from Virginia and one from Oklahoma, were used for these studies. In two separate trials, cattle inoculated with equal doses of the two isolates developed infection with only one genotype. Tick cell cultures inoculated with equal doses of the two isolates became infected with only the Virginia isolate of A. marginale. When cultures were inoculated with different ratios of the Oklahoma and Virginia isolates of A. marginale, the isolate inoculated in the higher ratio became established and excluded infection with the other. When cultures with established infections of one isolate were subsequently infected with the other, only the established isolate was detected. We documented infection exclusion during initial infection in cell culture by labeling each isolate with a different fluorescent dye. After 2 days in culture, only a single isolate was detected per cell by fluorescence microscopy. Finally, when Anaplasma ovis infections were established in cultures that were subsequently inoculated with the Virginia or Oklahoma isolate of A. marginale, A. marginale infection was excluded. These studies confirm that infection exclusion occurs with A. marginale in bovine erythrocytes and tick cells, resulting in the establishment of only one genotype, and appears to be the first report of infection exclusion for Anaplasma and Ehrlichia species.

scholarly journals Interleukin-12 as an Adjuvant Promotes Immunoglobulin G and Type 1 Cytokine Recall Responses to Major Surface Protein 2 of the Ehrlichial Pathogen Anaplasma marginale

2000 ◽  
Vol 68 (1) ◽  
pp. 270-280 ◽  
Author(s):  
Wenbin Tuo ◽  
Guy H. Palmer ◽  
Travis C. McGuire ◽  
Daming Zhu ◽  
Wendy C. Brown
Keyword(s):  
Protective Immunity ◽  
Mononuclear Cells ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Interleukin 12 ◽  
Major Surface Protein ◽  
Ifn Γ ◽  
Major Surface ◽  
Type 1 Cytokine

ABSTRACT Anaplasma marginale is a tick-transmitted pathogen of cattle closely related to the human ehrlichiae, Ehrlichia chaffeensis and the agent of human granulocytic ehrlichiosis (HGE). These pathogens have in common a structurally conserved outer membrane protein (OMP) designated the major surface protein 2 (MSP-2) in A. marginale and HGE and OMP-1 in E. chaffeensis. Protective immunity against ehrlichial pathogens is believed to require induction of gamma interferon (IFN-γ) and opsonizing immunoglobulin (Ig) subclasses directed against OMP epitopes that, in concert, activate macrophages for phagocytosis and killing. Because interleukin-12 (IL-12) acts as an adjuvant for protein immunization to induce IFN-γ and protective immunity against intracellular pathogens, we hypothesized that as an adjuvant with MSP-2, IL-12 would augment type 1 recall responses to A. marginale. IL-12 was coadsorbed with MSP-2 to alum and shown to significantly enhance IFN-γ production by lymph node cells (LNC) and LNC-derived CD4+ T-cell lines from immunized calves following recall stimulation with A. marginale. LNC proliferation and IL-2 production were also enhanced in IL-12-treated calves. Elevated recall proliferative responses by peripheral blood mononuclear cells were still evident 9 months after immunization. Serum IgG levels were consistently increased in IL-12 immunized calves, predominantly due to higher IgG1 responses. The results support the use of IL-12 coadsorbed with OMP of ehrlichial pathogens in alum to amplify both antibody and type-1 cytokine responses important for protective immunity.

scholarly journals Characterization of Anaplasma phagocytophilum Major Surface Protein 5 and the Extent of Its Cross-Reactivity with A. marginale

2007 ◽  
Vol 14 (3) ◽  
pp. 262-268 ◽  
Author(s):  
N. I. Strik ◽  
A. R. Alleman ◽  
A. F. Barbet ◽  
H. L. Sorenson ◽  
H. L. Wamsley ◽  
...  
Keyword(s):  
United States ◽  
Anaplasma Phagocytophilum ◽  
Indirect Elisa ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
The United States ◽  
Cross Reactivity ◽  
Serum Samples ◽  
Major Surface Protein ◽  
Major Surface

ABSTRACT Major surface protein 5 (Msp5) of Anaplasma marginale is highly conserved in the genus Anaplasma and the antigen used in a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for serologic identification of cattle with anaplasmosis. This study analyzes the degrees of conservation of Msp5 among various isolates of Anaplasma phagocytophilum and the extent of serologic cross-reactivity between recombinant Msp5 (rMsp5) of Anaplasma marginale and A. phagocytophilum. The msp5 genes from various isolates of A. phagocytophilum were sequenced and compared. rMsp5 proteins of A. phagocytophilum and A. marginale were used separately in an indirect ELISA to detect cross-reactivity in serum samples from humans and dogs infected with A. phagocytophilum and cattle infected with A. marginale. Serum samples were also tested with a commercially available competitive ELISA that uses monoclonal antibody ANAF16C1. There were 100% sequence identities in the msp5 genes among all of the A. phagocytophilum isolates from the United States and a horse isolate from Sweden. Sheep isolates from Norway and dog isolates from Sweden were 99% identical to one another but differed in 17 base pairs from the United States isolates and the horse isolate. Serologic cross-reactivity was identified when serum samples from cattle infected with A. marginale were reacted with rMsp5 of A. phagocytophilum and when serum samples from humans and dogs infected with A. phagocytophilum were reacted with rMsp5 of A. marginale in an indirect-ELISA format. Serum samples from dogs or humans infected with A. phagocytophilum did not cross-react with rMsp5 of A. marginale when tested with the commercially available cELISA. These results suggest that rMsp5 of A. phagocytophilum is highly conserved among United States and European isolates and that serologic distinction between A. phagocytophilum and A. marginale infections cannot be accomplished if rMsp5 from either organism is used in an indirect ELISA.

Characterization of the functional domain of major surface protein 1a involved in adhesion of the rickettsia Anaplasma marginale to host cells

2003 ◽  
Vol 91 (2-3) ◽  
pp. 265-283 ◽  
Author(s):  
José de la Fuente ◽  
Jose C Garcia-Garcia ◽  
Edmour F Blouin ◽  
Katherine M Kocan
Keyword(s):  
Surface Protein ◽  
Anaplasma Marginale ◽  
Host Cells ◽  
Functional Domain ◽  
Major Surface Protein ◽  
Major Surface

Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences

2007 ◽  
Vol 119 (2-4) ◽  
pp. 382-390 ◽  
Author(s):  
José de la Fuente ◽  
Paula Ruybal ◽  
Moses S. Mtshali ◽  
Victoria Naranjo ◽  
Li Shuqing ◽  
...  
Keyword(s):  
Surface Protein ◽  
Anaplasma Marginale ◽  
Major Surface Protein ◽  
Repeat Sequences ◽  
Major Surface
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