scholarly journals OPTIMIZATION OF THE ACID HYDROLYSIS OF CLADODES OF Opuntia ficus-indica BY RESPONSE SURFACE METHODOLOGY

2018 ◽  
Vol 17 (3) ◽  
pp. 1095-1104
Author(s):  
A. Texco ◽  
Author(s):  
Ebrahim Eskandari Nasab ◽  
Mehran Habibi-Rezaei ◽  
Afshin Khaki ◽  
Mohammad Balvardi

In this study, the acid hydrolysis of inulin was investigated as a function of three variables: pH, temperature and time. Inulin hydrolysis was detected by measurement of reducing sugars, using Dinitro Salicylic acid (DNS) method. The central composite rotatable design (CCRD) was applied to design an experimental program to model the effects of acidic and alkaline pH on the inulin hydrolysis. Additionally, response surface methodology (RSM) was utilized for data analysis. The statistical analysis of the results confirmed that pH, temperature and time are significant variables at acidic pH, whereas at alkaline pH, these variables are insignificant. The maximum amount of inulin hydrolysis obtained at the pH < 2, temperature > 90°C and the time of 1 hrs.


2013 ◽  
Vol 699 ◽  
pp. 77-82 ◽  
Author(s):  
S. Duangwang ◽  
C. Sangwichien

Oil palm empty fruit bunch is a lignocellulosic material from palm oil plantations. It is a potential source of xylose which can be used as a raw material for production of xylitol. Using of lignocellulosic waste for bioconversion to fuels and chemicals is justifiable as these materials are low cost, renewable and widespread sources of sugars. The objective of the present study was to determine the effect of H2SO4 concentration, reaction temperature and reaction time for acid hydrolysis of pretreated OPEFB, pretreated OPEFB with reaction temperature, reaction time and NaOH concentration were 130 °C, 40 min and 15% (w/v), respectively to achieve high xylose yield. Batch reactions were carried out under various reaction temperature, reaction time and H2SO4 concentration. Response Surface Methodology (RSM) was followed to optimize acid hydrolysis in order to obtain high yield of xylose. The optimum reaction temperature, reaction time and H2SO4 concentration were found to be 140 °C, 90 min and 7% (w/v), respectively. The maximum value of xylose was obtained 56.39 g/l by using the above condition. The best result of xylose yield obtained was 126%.


2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.


2018 ◽  
Vol 52 (6) ◽  
pp. 581-587
Author(s):  
Vinayaka B. Shet ◽  
Nisha sanil ◽  
Manasa Bhat ◽  
Manasa Naik ◽  
Leah Natasha Mascarenhas ◽  
...  

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