Inhibition Effect of Gold (III) Theophylline Nano-complex on ALT Enzyme Activity in Human Serumof Iraqi Patients with Liver Disease

Introduction: The ultrasonic sonication approach was used to create an Au(III) nano complex with theophylline. Methods: To explore and suggest the structure of the nano complex, UV-Vis spectroscopy, Fourier transform infrared (FT-IR), and carbon hydrogen and nitrogen (CHN) elemental studies were used. The FE-SEM was used to prove the nanoscale of the prepared complex and was to be less than 20 nm. The effect of the gold nano complex (Au (THP)2(Cl)2) on alanine transaminase (ALT) activity in the serum of chronic liver disease patients was investigated. Results: Compared to the control group, the patients with chronic liver disease with and without nano complex had a significant rise in serum levels of ALT activity (P < 0.001). Furthermore, in individuals with chronic liver disease who received nano complex, the blood levels of ALT activity were significantly lower than those who did not receive nano complex. The reason is that the Au nano complex aggressively interacts with carboxylic groups of important enzymes and inactivates them; further, the Au nano complex had an inhibitory effect on serum ALT activity.

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Chronic Alcoholism Decreases Polyunsaturated Fatty Acid Levels in Human Plasma, Erythrocytes, and Platelets – Influence of Chronic Liver Disease

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657633 ◽

1997 ◽

Vol 78 (02) ◽

pp. 808-812 ◽

Cited By ~ 10

Author(s):

María-Luisa Pita ◽

José-María Rubio ◽

María-Luisa Murillo ◽

Olimpia Carreras ◽

Mariá-José Delgado

Keyword(s):

Fatty Acid ◽

Liver Disease ◽

Chronic Liver Disease ◽

Chronic Alcoholism ◽

Chronic Liver ◽

Chronic Ethanol ◽

Ethanol Ingestion ◽

Control Group ◽

Docosatetraenoic Acid ◽

Long Chain

SummaryThe effect of chronic ethanol ingestion on fatty acid composition of plasma, erythrocyte and platelet phospholipids and on plasma 6-keto-PGF1α was studied. Two groups of alcoholic subjects, one of them with chronic liver disease, were studied and compared to a control group of healthy subjects. Linoleic acid was not affected by alcoholism but its larger metabolites arachidonic acid (20:4n6) and docosatetraenoic acid (22: 4n6) tended to be lower in erythrocytes and platelets of both groups of alcoholic patients; the decrease was more marked in the presence of chronic liver disease. Docosahexaenoic acid (22:6n3) was markedly decreased in plasma, erythrocytes and platelets obtained from alcoholic patients with chronic liver disease. Plasma levels of 6-keto-PGF1α, a metabolite of prostacyclin (PGI2), remained unchanged. We conclude that chronic ethanol ingestion induces important changes in long-chain polyunsaturated fatty acids, mainly in platelets, and that these changes are exacerbated when patients suffer from chronic liver disease.

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Procollagen peptide serum levels in chronic liver disease: Comparison of antibody — And FAB — Radioimmunoassays for assessment of liver fibrosis

Journal of Hepatology ◽

10.1016/s0168-8278(85)80737-6 ◽

1985 ◽

Vol 1 ◽

pp. S351

Keyword(s):

Liver Disease ◽

Liver Fibrosis ◽

Chronic Liver Disease ◽

Serum Levels ◽

Chronic Liver

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Immature Platelet Fraction in Patients with Chronic Liver Disease, A Marker for Evaluating Cirrhotic Changes.

Annals of PIMS-Shaheed Zulfiqar Ali Bhutto Medical University ◽

10.48036/apims.v17i2.531 ◽

2021 ◽

Vol 17 (2) ◽

pp. 174-179

Author(s):

Muhammad Javaid Iqbal ◽

Muhammad Usman ◽

Mubarak Ali Anjum ◽

Yasir Yaqoob ◽

Ghulam Mujtaba Nasir ◽

...

Keyword(s):

Platelet Count ◽

Liver Disease ◽

Chronic Liver Disease ◽

White Blood Cells ◽

Chronic Liver ◽

Control Group ◽

P Value ◽

Master Program ◽

Immature Platelet Fraction ◽

The Mean

Objective: To evaluate the role of Immature platelet fraction in patients with chronic liver disease, a marker for evaluating cirrhotic changes. Methodology: This case control study was conducted at department of Pathology, Aziz Fatima Medical and Dental College, Faisalabad, over a period of Seven months from June 2020 to January 2021. A total of 126 participants were included in the study consisting of 63 patients with chronic liver disease in group A and 63 participants without any known disease in group B as control. The IPF master program in combination with XE-2100 multiparameter automatic hematology analyzer was used to measure the immature platelet fraction. Ethylene diamine tetraacetic acid was used to collect the blood sample for IPF measurement and was maintained till analysis on room temperature. Ten repeated analyses, immediately and after 24 hours were done for reproducibility of IPF%. Results: The mean age of liver disease patients was 52.35 ± 13.64 years and in control group the mean age was 51.62 ± 11.27 years. There was no significant (p-value > 0.05) difference between both groups based on age and gender. The hemoglobin level and red cell count was found to be significantly (p-value < 0.05) reduced in cases group. While white blood cells count was comparable in both groups. The mean platelet count was significantly (p-value < 0.05) less in cases group (163.5 ± 90.4 vs 233.4 ± 54.5 (x10*3/µl). The mean value of immature platelet fraction (IPF%) was significantly (p-value < 0.05) raised in cases group (5.62 ± 2.92 vs 3.06 ± 1.87). The multivariate discriminant analysis (MDA) score showed a significant (p-value < 0.05) association with chronic hepatis as compared to other liver related diseases. Conclusions: In chronic liver disease patients, there is an inverse relationship between platelet count and IPF% with decreased platelet count and increased IPF%. The proposed MDA function can be used to identify the cirrhotic changes in liver disease patients.

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Serum levels of anti-heat shock protein 27 antibodies in patients with chronic liver disease

Cell Stress and Chaperones ◽

10.1007/s12192-020-01164-3 ◽

2020 ◽

Vol 26 (1) ◽

pp. 151-157

Author(s):

Gabriella Gruden ◽

Patrizia Carucci ◽

Federica Barutta ◽

Davina Burt ◽

Arianna Ferro ◽

...

Keyword(s):

Liver Disease ◽

Heat Shock ◽

Heat Shock Protein ◽

Chronic Liver Disease ◽

Serum Levels ◽

Heat Shock Protein 27 ◽

Chronic Liver

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Under-Vaccination in Pediatric Liver Transplant Candidates with Acute and Chronic Liver Disease—A Retrospective Observational Study of the European Reference Network TransplantChild

Children ◽

10.3390/children8080675 ◽

2021 ◽

Vol 8 (8) ◽

pp. 675

Author(s):

Tobias Laue ◽

Zeynep Demir ◽

Dominique Debray ◽

Mara Cananzi ◽

Paola Gaio ◽

...

Keyword(s):

Liver Disease ◽

Liver Transplant ◽

Chronic Liver Disease ◽

Vaccination Coverage ◽

Chronic Liver ◽

Control Group ◽

Reference Network ◽

Pediatric Liver ◽

Transplant Candidates ◽

Pediatric Liver Transplant

Infection is a serious concern in the short and long term after pediatric liver transplantation. Vaccination represents an easy and cheap opportunity to reduce morbidity and mortality due to vaccine-preventable infection. This retrospective, observational, multi-center study examines the immunization status in pediatric liver transplant candidates at the time of transplantation and compares it to a control group of children with acute liver disease. Findings show only 80% were vaccinated age-appropriately, defined as having received the recommended number of vaccination doses for their age prior to transplantation; for DTP-PV-Hib, less than 75% for Hepatitis B and two-thirds for pneumococcal conjugate vaccine in children with chronic liver disease. Vaccination coverage for live vaccines is better compared to the acute control group with 81% versus 62% for measles, mumps and rubella (p = 0.003) and 65% versus 55% for varicella (p = 0.171). Nevertheless, a country-specific comparison with national reference data suggests a lower vaccination coverage in children with chronic liver disease. Our study reveals an under-vaccination in this high-risk group prior to transplantation and underlines the need to improve vaccination.

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Biomarkers of Angiogenesis in Hepatocellular Carcinoma: A Novel Sunshine Road

10.20944/preprints202109.0502.v1 ◽

2021 ◽

Author(s):

Krizia Pocino ◽

Cecilia Napodano ◽

Gabriele Ciasca ◽

Mariapaola Marino ◽

Nicoletta De Matthaeis ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

Liver Disease ◽

Chronic Liver Disease ◽

Venous Blood ◽

Serum Levels ◽

Chronic Liver ◽

Number Of Factors ◽

Measurement Results ◽

Before And After

Background: Hepatocellular carcinoma (HCC) is a global health problem associated with chronic liver disease. The pathogenesis of chronic liver disease varies according to the underlying etiological factor, although in most cases it develops from a liver cirrhosis. The worsening progression of liver disease is accompanied by pathological angiogenesis, which is a prerequisite that favors the development of HCC. The aim of this study is to evaluate the clinical utility of circulating angiogenic markers VEGF, Ang-1, Ang-2, the Angiopoietin receptor (Tie1/2), HGF and PECAM-1 to screen early onset patients and to follow the evolution of HCC. Materials and Methods: We enrolled 62 patients; 33 out of 62 subjects were diagnosed for HCC and 29/62 for liver cirrhosis of different etiology without signs of neoplasia. Patients underwent venous blood sampling before and after treatments for VEGF, Ang-1, Ang-2, Tie1, Tie2, HGF and PECAM-1 measurement. Results: Ang-1 and Ang-2 are detectable not only in patients already suffering from HCC but also in cirrhotic patients without signs of cancer. Patients with HCC show higher HGF concentrations than patients with cirrhosis. A significant reduction in serum levels of Ang-2, Ang-2/Ang-1 and Ca 19-9 after DAAs therapy was observed. Moreover, VEGF levels were increased after treatment of HCC. Conclusion: The preliminary study here presented confirms that the mechanism of tumor angiogenesis is very complex and involves a very large number of factors. The integration of different methodologies and multi-marker algorithms is likely to emerge for the early diagnosis of HCC and the monitoring of the risk of relapse.

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Genetic Susceptibility to Chronic Liver Disease in Individuals from Pakistan

International Journal of Molecular Sciences ◽

10.3390/ijms21103558 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3558

Author(s):

Asad Mehmood Raja ◽

Ester Ciociola ◽

Imran Nazir Ahmad ◽

Faisal Saud Dar ◽

Syed Muhammad Saqlan Naqvi ◽

...

Keyword(s):

Liver Disease ◽

Genetic Susceptibility ◽

Chronic Liver Disease ◽

Disease Risk ◽

Protective Role ◽

Chronic Liver ◽

Control Group ◽

Chi Square ◽

Logistic Analysis ◽

Common Genetic Variants

Chronic liver disease, with viral or non-viral etiology, is endemic in many countries and is a growing burden in Asia. Among the Asian countries, Pakistan has the highest prevalence of chronic liver disease. Despite this, the genetic susceptibility to chronic liver disease in this country has not been investigated. We performed a comprehensive analysis of the most robustly associated common genetic variants influencing chronic liver disease in a cohort of individuals from Pakistan. A total of 587 subjects with chronic liver disease and 68 healthy control individuals were genotyped for the HSD17B13 rs7261356, MBOAT7 rs641738, GCKR rs1260326, PNPLA3 rs738409, TM6SF2 rs58542926 and PPP1R3B rs4841132 variants. The variants distribution between case and control group and their association with chronic liver disease were tested by chi-square and binary logistic analysis, respectively. We report for the first time that HSD17B13 variant results in a 50% reduced risk for chronic liver disease; while MBOAT7; GCKR and PNPLA3 variants increase this risk by more than 35% in Pakistani individuals. Our genetic analysis extends the protective role of the HSD17B13 variant against chronic liver disease and disease risk conferred by the MBOAT7; GCKR and PNPLA3 variants in the Pakistani population.

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P-019 Use of serum levels of cytokine TGF &bgr;1 in detection of Hepatocellular carcinoma in patients with chronic liver disease

Annals of Oncology ◽

10.1093/annonc/mdw199.19 ◽

2016 ◽

Vol 27 ◽

pp. ii6

Author(s):

A. Mehmedovic ◽

B. Prnjavorac ◽

Z. Vukobrat- Bijedić

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Disease ◽

Chronic Liver Disease ◽

Serum Levels ◽

Chronic Liver

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Carbohydrate 19.9 Antigen Serum Levels in Liver Disease

BioMed Research International ◽

10.1155/2013/531640 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 19

Author(s):

Gaetano Bertino ◽

Annalisa Maria Ardiri ◽

Giuseppe Stefano Calvagno ◽

Giulia Malaguarnera ◽

Donatella Interlandi ◽

...

Keyword(s):

Chronic Hepatitis ◽

Liver Disease ◽

Chronic Liver Disease ◽

Serum Levels ◽

Chronic Viral Hepatitis ◽

Chronic Liver ◽

Neoplastic Disease ◽

Prospective Longitudinal Study ◽

Ca 19.9 ◽

Prospective Longitudinal

Background.Carbohydrate 19.9 antigen (CA19.9) has been used in the diagnosis and followup of gastrointestinal tumours. The aim of this prospective longitudinal study was the evaluation of CA19.9 levels in patients with chronic hepatitis and hepatic cirrhosis hepatitis C virus and B virus correlated.Materials and Methods. 180 patients were enrolled, 116 with HCV-related chronic liver disease (48% chronic hepatitis, 52% cirrhosis) and 64 with HBV-related chronic liver disease (86% chronic hepatitis, 14% cirrhosis). Patients with high levels of CA19.9 underwent abdominal ecography, gastroendoscopy, colonoscopy, and abdominal CT scan.Results.51.7% of patients with HCV-related chronic liver disease and 48.4% of those with HBV-related chronic liver disease presented high levels of CA19.9. None was affected by pancreatic or intestinal neoplasia, cholestatic jaundice, or other diseases potentially able to induce Ca19.9 elevations. CA19.9 levels were elevated in 43.3% of HCV chronic hepatitis, in 56.3% of HCV cirrhosis, in 45.1% of HBV chronic hepatitis, and in 58% of HBV cirrhosis.Conclusions.CA19.9 commonly increases in the serum of patients with chronic viral hepatitis. Elevation of CA 19.9 is not specific for neoplastic disease and is related to the severity of fibrosis and to the viral aetiology of hepatitis.

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