scholarly journals MICROPROPAGATION STUDIES AND ANTIOXIDANT ANALYSIS OF THE ENDANGERED PLANTS OF BULGARIAN YELLOW GENTIAN (Gentiana lutea L.)

2019 ◽  
Vol 18 (3) ◽  
pp. 71-78
Author(s):  
Maria I. Petrova ◽  
Ely G. Zayova ◽  
Lyudmila I. Dimitrova ◽  
Maria P. Geneva ◽  
Kamelia D. Miladinova-Georgieva
Keyword(s):  
Growth Regulators ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Guaiacol Peroxidase ◽  
Nutrient Media ◽  
Shoot Height ◽  
Gentiana Lutea ◽  
Antioxidant Metabolites ◽  
Rare And Endangered

In order to develop an efficient micropropagation system, it is essential to establish the appropriate concentration of growth regulators for seed germination, shoot formation and rooting. Nodal segments from in vitro obtained seedlings of Gentiana lutea L. were cultured in vitro in Murashige and Skoog’s medium supplemented with BAP, Thidiazuron and Zeatin (0.5, 1.0 and 2.0 mg L−1). A maximum number of shoots with the highest height was recorded at 2.0 mg L−1 BAP. For further optimization of the process, we used nutrient media containing BAP and Zeatin with a combination of low concentration of Indoleacetic acid. MS medium containing 2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA resulted in maximum numbers of shoots 94.3) with shoot height 2.5 cm. The multiple plants were successfully ex vitro acclimatized with 65% survival. The presence of growth regulators (2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA) in the nutrient media resulted in an effective antioxidant activity in G. Lutea determined by the low molecular antioxidant metabolites such as phenols and flavonoids and activities of antioxidant enzymes – catalase, ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase. The described protocol allows the establishment of numerous micropropaged plants of rare and endangered G. lutea.

scholarly journals REGENERATION CAPACITY OF NARROW-LOCALIZED ENDEMIC SPECIES Dianthus hypanicus Andrz. in vitro

2021 ◽  
Vol 14 (3) ◽  
pp. 39-45
Author(s):  
L.A. Koldar ◽  
Keyword(s):  
Growth Regulators ◽  
Nutrient Medium ◽  
Shoot Formation ◽  
Natural Habitats ◽  
Nutrient Media ◽  
Leafy Shoots ◽  
Mass Reproduction ◽  
Rare And Endangered ◽  
Propagation Methods

Development of effective technology of rare and endangered plant species mass reproduction in vitro is one of the urgent nowadays tasks. Diantus hypanicus Andrz. of the Caryophyllaceae Juss. family belongs to this group of plants. It is an endemic, narrow-localized species listed in the Red Book of Ukraine, the European Red List and the Annex to the Berne Convention. Prospects for the conservation of this species are associated with the study of its viability and require the development of effective reproduction methods. Aim. The purpose of the experiment was to determine the effect of different growth regulators concentrations on the D. hypanicus explants regenerative capacity during in vitro propagation. Methods. For the experiment the seed were used collected from plants of natural habitats and sown on a hormone-free nutrient medium according to the recipe of Murashige and Skoog. For 12–15 days, 4–6 cm long seedlings were obtained which were transferred to nutrient media modified with the content of growth-regulating substances: BAP 0.5–2.0 mg / l, IBA 0.01 mg / l and IAA 0.1 mg / l. Results. Initiation of adventitious buds with subsequent shoot formation was observed for 12–16 days. Each variant plants differed in number, growth activity and intensity, morphometric parameters. After 35–40 days from the explant introduction, conglomerates with well-developed leafy shoots were formed. It was found that, when modifying nutrient media with growth regulators BAP, IBA, IAA of different concentrations, D. hypanicus explants were actively undergoing regeneration processes of different intensity. Conclusions. It was proved that high ability to regenerate was the characteristic of explants cultured on a nutrient medium modified by the addition of BAP – 0.5 mg / l and IAA – 0.1 mg / l, where, on average, 18 ± 0.24 shoots of 2.42 ± 0.17 cm long were formed with a net reproduction of 19.06 ± 0.14. Shoots with 3.0–5.0 pairs of leaves that reached 3.5–6.0 cm were selected and transferred to nutrient media for rooting. Less developed shoots were planted on nutrient media for further reproduction. The next stage of the study is aimed at modifying nutrient media to achieve explants rhizogenesis and obtain a large number of plants necessary for the preservation of this endemic narrow-localized species in culture with subsequent repatriation to natural places of growth.

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

scholarly journals Genetic Variability and Heritability of in vitro Chrysanthemum on Various Combination of Plant Growth Regulators

2018 ◽  
Vol 3 (2) ◽  
pp. 69-77
Author(s):  
Muh. Farid Bdr
Keyword(s):  
Genetic Variability ◽  
Growth Regulators ◽  
Culture Media ◽  
Coconut Water ◽  
Shoot Height ◽  
High Heritability ◽  
Main Plot ◽  
Department Faculty ◽  
Plot Design

Heritability is a measure that describes the magnitude of a genetic influence on a character rather than an environmental factor. The higher the heritability value of a character, the higher the genetic variability. The aim of the study was to know the interaction between the combination of growth regulators and chrysanthemum varieties that gave the best chrysanthemum growth in vitro and analyzed the heritability of various chrysanthemum characters. The research was carried out at the In vitro Laboratory of Agronomy Department, Faculty of Agriculture, Hasanuddin University, Makassar from May to September 2016. The study used a separate plot design in the group. The main plot was a combination of growth regulator consisting of 200 ml Coconut Water, 0.5 ppm BAP + Coconut Water 100 ml, BAP 1 ppm + Coconut Water 100 ml, 2,4-D 0.5 ppm + Coconut Water 200 ml, 2,4-D 0.5 ppm + BAP 0.5 ppm + Coconut Water 100 ml, 2,4-D 0.5 ppm + BAP 1 ppm + Coconut Water 100 ml. The subplots included 8 varieties of chrysanthemum (Fiji Pink, Marimar, Pasopati, Salzieta, Solida Pelangi, Limeron, Arosuka Pelangi, Yellow Tumohon). The basic media used was MS medium. The results showed that adding 200 ml young coconut to the culture media gave the best in-vitro chrysanthemum growth in terms of shoot and root formation, shoot count, root number, and shoot height in all chrysanthemum varieties; as well as all observed characters have high heritability and genetic variability

scholarly journals Callus Induction and Shoot Formation for Mexican Red Bean (Phaseolus vulgaris L.) Pinto Cultivar in Vitro

2020 ◽  
pp. 1887-1893
Author(s):  
Rasha K. Mohammed Al-Saedi ◽  
Ansam G. Abdulhalem
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Phaseolus Vulgaris L ◽  
Different Types ◽  
Red Bean ◽  
Internode Explants

     The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as  internodes, cotyledons and roots,  were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.

scholarly journals In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta)

HortScience ◽  
2012 ◽  
Vol 47 (1) ◽  
pp. 84-87 ◽  
Author(s):  
Milorad Vujičić ◽  
Aneta Sabovljević ◽  
Jasmina Šinžar-Sekulić ◽  
Marijana Skorić ◽  
Marko Sabovljević
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
Ex Situ ◽  
High Mountain ◽  
Natural Habitats ◽  
Rare And Endangered ◽  
Half Strength ◽  
Vitro Development ◽  
Maximum Development

The high mountain pottioid moss Molendoa hornschuchiana (Hook) Lindb. ex Limpr. is a very rare and critically endangered bryophyte species in Europe in need for ex situ conservation. A 25-year-old herbarium sample was used to revive and propagate this species for further reintroduction and introduction to potential natural habitats. The reviving of “dead” herbarium specimen was achieved by disposing of axenical organisms as well as adjusting condition for developing secondary protonema, bud inductions, and optimization of gametophyte propagation in vitro condition.The influence of exogenously added growth regulators on the morphogenesis of this species was studied. The plants were cultured in the two basic types of media, viz., BCD and half-strength Murashige and Skoog (MS) supplemented with different concentrations (0.01–0.3 μM) of indole-3-butyric acid (IBA) and N6-benzyladenine (BA) under a 16-h photoperiod. The influence of growth regulators on gametophores multiplication in vitro as well as on protonemal diameter was recorded. Well-developed gametophores were obtained on BCD medium, whereas on half-strength MS medium, secondary protonema was produced, both on hormone-free and supplemented substrate exclusively. Based on multiplication index in vitro, maximum development of gametophores was realized on BCD medium supplemented with 0.3 μM IBA and 0.1 μM BA. However, the widest diameter of secondary protonema was obtained on BCD medium enriched with low concentration of both BA (0.01 and 0.03 μM) and constant concentration of IBA (0.03 μM). Chemical names used: indole-3-butyric acid (IBA), N6-benzyladenine (BA), Murashige and Skoog medium (MS).

scholarly journals In vitro culture of jambu with different growth regulators

2020 ◽  
Vol 38 (2) ◽  
pp. 134-138
Author(s):  
Susan P Almeida ◽  
Joanne MM Souza ◽  
Andredy MT Amorim ◽  
Sérgio AL de Gusmão ◽  
Rodrigo ORM Souza ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Nodal Segments ◽  
Root Production ◽  
In Vitro Cultivation ◽  
Average Height ◽  
Ms Medium ◽  
Fresh Matter ◽  
Friable Callus

ABSTRACT The aim of this study was to establish the best concentrations of growth regulators for in vitro cultivation of jambu for a subsequent elaboration of an efficient micropropagation protocol. After sterilized, the seeds were inoculated on different media (MS, ½MS and water-agar) for in vitro germination. Nodal segments of in vitro germinated jambu seedlings were used as explants in the micropropagation with different concentrations of 6-benzylaminopurine (BAP) (0.0; 0.125; 0.25; 0.50 and 0.75 mg L-1) and callus induction with 2.4- dichlorophenoxyacetic acid (2.4-D) (0.0; 0.25; 0.50; 0.75 and 1.0 mg L-1) on Murashige & Skoog’s (MS) medium. The highest germination rates were obtained on MS medium with better seedling development and greater height (3.7cm). In micropropagation, the best treatment was obtained on 0.125 BAP (T2), with an average of 2.2 sprouts/explant, average height of 2.4 cm and vigorous sprouts. In callus induction, all treatments with 2.4-D had developed friable calluses in 30 days and using doses of 0.25 and 0.50 mg L-1 provided greater fresh matter. The induction of friable callus and the root production occur without supplementation of exogenous growth regulator.

scholarly journals Studying on the in vitro shoot formation of Opuntia ficus – indica (L.) Mill.

2019 ◽  
Vol 3 (1) ◽  
pp. 18-28
Author(s):  
Nguyen Thi Cam Duyen ◽  
Bui Trang Viet ◽  
Tran Thanh Huong
Keyword(s):  
Acetic Acid ◽  
Respiration Rate ◽  
Shoot Formation ◽  
Photosynthesis Rate ◽  
Naphthalene Acetic Acid ◽  
Opuntia Ficus Indica ◽  
Shoot Height ◽  
Explant Position ◽  
Meristematic Region

This study presented the elimination of the glochids in the areoles of Opuntia ficus-indica cladode induced the shoot formation from the explants containing the shoot apical meristem (SAM) on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) 5 mg/L. Morphological and physiological changes in shoot formation process were analyzed. This process involved the constant stages: activation of cell division, initiating of meristematic region; formation of shoot primordium and shoot with leaves. The SAM position in the upper front of the cladode gave the highest shoot productivity. This position had the photosynthesis rate, respiration rate, endogenous indole acetic acid (IAA) and zeatin activity higher than the others. Difference of auxin concentrations increased the amount of shoots and shoot height. The maximum number of shoots per explant was obtained on MS medium supplemented with BA 5 mg/L and 1-naphthalene acetic acid (NAA) 0.5 mg/L. The elimination of SAM by removing the surface of the shoot gave the highest number of the shoot. The correlation of the glochids in the areoles, explant position, plant hormones, photosynthesis rate respiration rate and shoot formation were discussed.  

scholarly journals Growth regulators and their reflection on different hop genotypes cultivated under in vitro conditions

2022 ◽  
Vol 82 ◽  
Author(s):  
R. de-Souza ◽  
C. R. Adams ◽  
R. C. de-Melo ◽  
A. F. Guidolin ◽  
A. Michel ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Culture Media ◽  
Variable Number ◽  
Nodal Segments ◽  
In Vitro Cultivation ◽  
Path Coefficient ◽  
Orthogonal Contrasts ◽  
Response Variables

Abstract Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.

scholarly journals COMBINATION OF NAA AND TDZ FOR In Vitro MULTIPLICATION OF Eugenia involucrata DC

2018 ◽  
Vol 41 (5) ◽  
Author(s):  
Diego Pascoal Golle ◽  
Lia Rejane Silveira Reiniger ◽  
Charlene Moro Stefanel ◽  
Marlove Fátima Brião Muniz ◽  
Karol Buuron da Silva
Keyword(s):  
Acetic Acid ◽  
Tree Species ◽  
Shoot Formation ◽  
Forest Tree ◽  
Ornamental Plant ◽  
Nodal Segments ◽  
In Vitro Multiplication ◽  
Forest Tree Species

ABSTRACT Eugenia involucrata DC. (Myrtaceae), an economically important forest tree species, is prized for its timber and fruits, and is also an important ornamental plant. This study aimed to evaluate the effect of Thidiazuron (TDZ) and α-Naphthaleneacetic acetic acid (NAA) on in vitro multiplication of nodal segments of E. involucrata. We tested the effect of the absence and presence of NAA (0.5 µM), combined with TDZ at concentrations of 0, 2, 4, 8, 16, or 32 µM, on the in vitro multiplication of E. involucrata. The use of TDZ combined with NAA (0.5 µM) favored the formation of shoots and buds in the explants, especially at 32 µM TDZ concentration. Intermediate concentrations of TDZ also promoted shoot formation but induced hyperhydricity in the explants. It is possible to induce organogenesis leading to the multiplication of E. involucrata nodal segments using TDZ, preferably combined with NAA.

Micropropagation of Highly Productive Forms of Diploid and Triploid Aspen

2014 ◽  
Vol 962-965 ◽  
pp. 681-690 ◽  
Author(s):  
Dmitriy Nikolaevich Zontikov ◽  
Svetlana Zontikova ◽  
Roman Sergeev ◽  
Alex Shurgin ◽  
M. Sirotina
Keyword(s):  
Growth Regulators ◽  
Source Material ◽  
Soil Conditions ◽  
Nutrient Media ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Triploid Clones

The stages of getting the planting material for laying plantations of the triploid aspen (Populustremula L.) are illustrated in this work. The source material selected from the genetic fund “The gigantic aspens” is characterized. The peculiarities of using the method of clonal micropropagation when getting the planting material, the usage of a rootstock as a source of donor explants for the introduction into the culture in vitro, the influence of a kind of nutrient media and growth regulators NAA and BAP on the growth and the development of microsprouts. The distinctions in the speed of the growth of the culture in vitro of diploid and triploid clones have been analyzed. The adaptation of the plants-regenerants to the soil-conditions.

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