scholarly journals Genetic Variability and Heritability of in vitro Chrysanthemum on Various Combination of Plant Growth Regulators

2018 ◽  
Vol 3 (2) ◽  
pp. 69-77
Author(s):  
Muh. Farid Bdr
Keyword(s):  
Genetic Variability ◽  
Growth Regulators ◽  
Culture Media ◽  
Coconut Water ◽  
Shoot Height ◽  
High Heritability ◽  
Main Plot ◽  
Department Faculty ◽  
Plot Design

Heritability is a measure that describes the magnitude of a genetic influence on a character rather than an environmental factor. The higher the heritability value of a character, the higher the genetic variability. The aim of the study was to know the interaction between the combination of growth regulators and chrysanthemum varieties that gave the best chrysanthemum growth in vitro and analyzed the heritability of various chrysanthemum characters. The research was carried out at the In vitro Laboratory of Agronomy Department, Faculty of Agriculture, Hasanuddin University, Makassar from May to September 2016. The study used a separate plot design in the group. The main plot was a combination of growth regulator consisting of 200 ml Coconut Water, 0.5 ppm BAP + Coconut Water 100 ml, BAP 1 ppm + Coconut Water 100 ml, 2,4-D 0.5 ppm + Coconut Water 200 ml, 2,4-D 0.5 ppm + BAP 0.5 ppm + Coconut Water 100 ml, 2,4-D 0.5 ppm + BAP 1 ppm + Coconut Water 100 ml. The subplots included 8 varieties of chrysanthemum (Fiji Pink, Marimar, Pasopati, Salzieta, Solida Pelangi, Limeron, Arosuka Pelangi, Yellow Tumohon). The basic media used was MS medium. The results showed that adding 200 ml young coconut to the culture media gave the best in-vitro chrysanthemum growth in terms of shoot and root formation, shoot count, root number, and shoot height in all chrysanthemum varieties; as well as all observed characters have high heritability and genetic variability

scholarly journals Genetic Variability and Heritability of in vitro Chrysanthemum on Various Combination of Plant Growth Regulators

2019 ◽  
Vol 3 (2) ◽  
pp. 69-77
Author(s):  
Muh Farid BDR
Keyword(s):  
Genetic Variability ◽  
Growth Regulators ◽  
Culture Media ◽  
Coconut Water ◽  
Shoot Height ◽  
High Heritability ◽  
Main Plot ◽  
Department Faculty ◽  
Plot Design

Heritability is a measure that describes the magnitude of a genetic influence on a character rather than an environmental factor. The higher the heritability value of a character, the higher the genetic variability. The aim of the study was to know the interaction between the combination of growth regulators and chrysanthemum varieties that gave the best chrysanthemum growth in vitro and analyzed the heritability of various chrysanthemum characters. The research was carried out at the In vitro Laboratory of Agronomy Department, Faculty of Agriculture, Hasanuddin University, Makassar from May to September 2016. The study used a separate plot design in the group. The main plot was a combination of growth regulator consisting of 200 ml Coconut Water, 0.5 ppm BAP + Coconut Water 100 ml, BAP 1 ppm + Coconut Water 100 ml, 2,4-D 0.5 ppm + Coconut Water 200 ml, 2,4-D 0.5 ppm + BAP 0.5 ppm + Coconut Water 100 ml, 2,4-D 0.5 ppm + BAP 1 ppm + Coconut Water 100 ml. The subplots included 8 varieties of chrysanthemum (Fiji Pink, Marimar, Pasopati, Salzieta, Solida Pelangi, Limeron, Arosuka Pelangi, Yellow Tumohon). The basic media used was MS medium. The results showed that adding 200 ml young coconut to the culture media gave the best in-vitro chrysanthemum growth in terms of shoot and root formation, shoot count, root number, and shoot height in all chrysanthemum varieties; as well as all observed characters have high heritability and genetic variability

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

In vitro multiplication and rooting of grapevine: Culture media and plant growth regulators

2019 ◽  
Vol 15 (1) ◽  
pp. 1-7
Author(s):  
Daniele Camargo Nascimento ◽  
Maximiliano Dini ◽  
Norton Victor Sampaio ◽  
Márcia Wulff Schuch
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
In Vitro Multiplication

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals In Vitro Propagation of Lilium longiflorum Bulbs Using NAA and BAP Plant Growth Regulator Treatment

2020 ◽  
Author(s):  
Ni Wayan Deswiniyanti ◽  
Ni Kadek Dwipayani Lestari
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Lilium Longiflorum ◽  
Herbaceous Plant ◽  
Perennial Herbaceous Plant ◽  
Plant Parts ◽  
Lily Bulb

Lily (Lilium longiflorum) is a perennial herbaceous plant with white trumpet-shaped flowers, fragrant and bulbous. In vitro culture through bulbs is one of way propagation of lily plants, but it requires a long time and only produces limited plants. In vitro propagation is a very promising technique for plant propagation because it can produce a lot of plant seeds in a short time. Bulbs are one of the fastest explants for growing shoots in lilies, but it is not known for certain which cuts of explants from bulb scales are best for multiplying in vitro. This study aims to determine the effect of lily bulb explants and the concentration of NAA and BAP growth regulators on the growth of lily bulb explants. The best results were obtained on the base and middle cuts explant of bulb scales compared to the tip cuts explant ones. The best results of the growing percentage, the number of shoots and the best growing time are shown in the combination treatment of growth regulator 1 mg L−1 NAA and 1 mg L−1 BAP. The optimum results on the number of micro bulbs were found in the treatment of growth regulators 0.5 mg L−1 NAA and 1 mg L−1 BAP. The best results of the average time formed micro bulb was in the treatment of 1 mg L−1 NAA and BAP with middle explant cuts, and treatment concentrations of 0.5 mg L−1 NAA and BAP in the base explant section. The base and middle bulb explants are able to regenerate or grow higher shoots. This is caused by the presence of endogenous natural auxin and the spread of auxin in plant parts not in the same amount. Therefore when added to the exogenous growth regulator such as auxin or cytokines to culture media will further trigger the formation of micro tubers more quickly,. It can increase the concentration of endogenous growth regulators in cells, help growing process and developing tissue.   Keywords: Bulb, lily, micro bulbs, in vitro, shoots

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

scholarly journals In vitro Propagation of Laelia albida (Orchidaceae) for Conservation and Ornamental Purposes in Mexico

HortScience ◽  
2005 ◽  
Vol 40 (2) ◽  
pp. 439-442 ◽  
Author(s):  
Leticia Santos-Hernández ◽  
Martha Martínez-García ◽  
Jorge E. Campos ◽  
Ernesto Aguirre-León
Keyword(s):  
Culture Media ◽  
Potato Starch ◽  
Seed Viability ◽  
Germination Percentage ◽  
Coconut Water ◽  
Naphthaleneacetic Acid ◽  
Monthly Basis ◽  
In Vitro Techniques ◽  
South Central

The orchid Laelia albida is an important cultural and religious plant resource of the Tehuacan-Cuicatlan Valley Biosphere Reserve in south-central Mexico. It is gradually becoming scarce due to overcollecting and habitat perturbation. For this reason, and for preservation purposes, the aim of the present work was to obtain the conditions to successfully propagate this species, through the use of mature seeds stored at 4 °C, and basal buds following in vitro techniques. Seeds with different storage periods (lot 1 and lot 2) were analyzed for seed viability as determined by the TTC method, germination percentage and germination index were analyzed on a monthly basis for 11 months on two Knudson C (K-4003, K-4128; Sigma). Seedlings were initially grown on the same culture media variants, but 30 days later they needed to be supplemented with potato starch (20 g·L-1). Basal buds were cultured on Knudson C containing nine BAP and NAA combinations two of which were also supplemented with potato starch (20 g·L-1) and coconut water (10%). Seed viability ranged from 78% to 98% throughout the 11 months and germination percentage was 70% to 90% without significant differences between the two lots. K-4003, either alone or supplemented with potato starch, was the best culture medium used to achieve all the development stages as well as seedlings with rhizoids. Bud proliferation was also successful on K-4003 added with BAP, NAA, potato starch and coconut water. The BAP (7.4 μm) and NAA (5.3 μm) combination promoted shoots and protocorm-like bodies (PLB). The results obtained with basal buds, make its preservation through in vitro culture possible and give the possibility to obtain available plantlets for its cultivation in regional nurseries. Storage conditions tested here may be useful for seed bank management for this species. Chemical names used: 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA).

scholarly journals Growth regulators and their reflection on different hop genotypes cultivated under in vitro conditions

2022 ◽  
Vol 82 ◽  
Author(s):  
R. de-Souza ◽  
C. R. Adams ◽  
R. C. de-Melo ◽  
A. F. Guidolin ◽  
A. Michel ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Culture Media ◽  
Variable Number ◽  
Nodal Segments ◽  
In Vitro Cultivation ◽  
Path Coefficient ◽  
Orthogonal Contrasts ◽  
Response Variables

Abstract Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.

scholarly journals MICROPROPAGATION STUDIES AND ANTIOXIDANT ANALYSIS OF THE ENDANGERED PLANTS OF BULGARIAN YELLOW GENTIAN (Gentiana lutea L.)

2019 ◽  
Vol 18 (3) ◽  
pp. 71-78
Author(s):  
Maria I. Petrova ◽  
Ely G. Zayova ◽  
Lyudmila I. Dimitrova ◽  
Maria P. Geneva ◽  
Kamelia D. Miladinova-Georgieva
Keyword(s):  
Growth Regulators ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Guaiacol Peroxidase ◽  
Nutrient Media ◽  
Shoot Height ◽  
Gentiana Lutea ◽  
Antioxidant Metabolites ◽  
Rare And Endangered

In order to develop an efficient micropropagation system, it is essential to establish the appropriate concentration of growth regulators for seed germination, shoot formation and rooting. Nodal segments from in vitro obtained seedlings of Gentiana lutea L. were cultured in vitro in Murashige and Skoog’s medium supplemented with BAP, Thidiazuron and Zeatin (0.5, 1.0 and 2.0 mg L−1). A maximum number of shoots with the highest height was recorded at 2.0 mg L−1 BAP. For further optimization of the process, we used nutrient media containing BAP and Zeatin with a combination of low concentration of Indoleacetic acid. MS medium containing 2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA resulted in maximum numbers of shoots 94.3) with shoot height 2.5 cm. The multiple plants were successfully ex vitro acclimatized with 65% survival. The presence of growth regulators (2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA) in the nutrient media resulted in an effective antioxidant activity in G. Lutea determined by the low molecular antioxidant metabolites such as phenols and flavonoids and activities of antioxidant enzymes – catalase, ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase. The described protocol allows the establishment of numerous micropropaged plants of rare and endangered G. lutea.

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