Micropropagation of Highly Productive Forms of Diploid and Triploid Aspen

2014 ◽  
Vol 962-965 ◽  
pp. 681-690 ◽  
Author(s):  
Dmitriy Nikolaevich Zontikov ◽  
Svetlana Zontikova ◽  
Roman Sergeev ◽  
Alex Shurgin ◽  
M. Sirotina
Keyword(s):  
Growth Regulators ◽  
Source Material ◽  
Soil Conditions ◽  
Nutrient Media ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Triploid Clones

The stages of getting the planting material for laying plantations of the triploid aspen (Populustremula L.) are illustrated in this work. The source material selected from the genetic fund “The gigantic aspens” is characterized. The peculiarities of using the method of clonal micropropagation when getting the planting material, the usage of a rootstock as a source of donor explants for the introduction into the culture in vitro, the influence of a kind of nutrient media and growth regulators NAA and BAP on the growth and the development of microsprouts. The distinctions in the speed of the growth of the culture in vitro of diploid and triploid clones have been analyzed. The adaptation of the plants-regenerants to the soil-conditions.

scholarly journals The study of the method of culture in vitro as a method of vegetative propagation of coniferous trees

2019 ◽  
pp. 19-22
Author(s):  
V. V. Krasnoperova ◽  
I. L. Bucharina
Keyword(s):  
Growth Regulators ◽  
Vegetative Propagation ◽  
Test Tube ◽  
Dichlorophenoxyacetic Acid ◽  
Nutrient Media ◽  
Culture In Vitro ◽  
Coniferous Trees ◽  
Planting Material ◽  
Economically Valuable Traits

Research is aimed at improving the technology of vegetative propagation of coniferous trees in vitro by selecting optimal media for sterilizing the explants of vegetative parts of plants, and using growth regulators to obtain callus and stimulating the root formation of test-tube plants. The objects of study are the vegetative parts of conifers: the buds and parts of the annual shoots. Plant objects sterilized and planted on nutrient media of different composition according to the scheme of experiments. Laboratory studies were conducted, on the basis of which successful use of sodium hypochlorite was noted compared with other reagents for sterilization of explants. According to the results of the experiments, a Woody Plant Medium nutrient medium was identified which promotes the best survival of the explants of conifers. The effect on growth survival of explants and their viability in culture in vitro, growth regulators such as 2.4-dichlorophenoxyacetic acid and 2.4-dinitrophenol in nutrient media was also determined. With the further cultivation and reproduction of coniferous plants, this technique allows to obtain planting material that most economically preserved in the offspring the economically valuable traits and properties of the parent plant.

scholarly journals Optimization of nutrient media for clonal micropropagation of the rootstock grape variety ‘Kober 5BB’

2020 ◽  
pp. 298-303
Author(s):  
Игорь Владимирович Гавриленко ◽  
Юлия Сергеевна Матяш ◽  
Анжела Владимировна Гавриленко ◽  
Дмитрий Александрович Шанин ◽  
Ирина Александровна Павлова ◽  
...  
Keyword(s):  
Culture Medium ◽  
Test Results ◽  
Grape Variety ◽  
Cultivation Conditions ◽  
Nutrient Media ◽  
Biometric Parameters ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Pathogenic Infection

Сорт винограда Кобер 5 ББ (Берландиери x Рипариа Кобер 5ББ) - один из основных подвоев, используемых в питомниководстве для получения привитых саженцев, поэтому в настоящее время крайне актуально создание маточников данного подвоя посадочным материалом категории «оригинальный». Это объясняет необходимость проведения исследований, связанных с оптимизацией условий культивирования сорта Кобер 5ББ, для повышения эффективности массового клонального микроразмножения с сохранением его генетической однородности и стабильности. Целью исследования являлась оптимизация и подбор питательных сред для клонального микроразмножения сорта-подвоя Кобер 5 ББ на этапе тиражирования (микрочеренкования). Материалом для исследований служили растения in vitro сорта подвоя Кобер 5ББ, свободные от основной патогенной инфекции (по результатам тестирования). Исследования проводили на средах: МS; WPM; DKW; PG (контроль). В качестве регуляторов роста использовали GA (гиббереллиновая кислота) в концентрациях: 0,2; 0,6; 1; 1,4 мг/л в сочетании с NAA (α-нафтилуксусная кислота) 0,05 мг/л. Показано, что растения на среде WPM, содержащей NAA-0,05 мг/л, по биометрическим показателям превосходили развившиеся на среде PG с аналогичным гормональным составом. Проведенные исследования по оптимизации среды культивирования для ускорения ростовых процессов позволили по результатам биометрических показателей выделить блок сред с основой WPM для размножения сорта-подвоя Кобер 5 ББ на этапе микрочеренкования. После проведения дополнительных исследований с расширенной выборкой среду WPM можно будет рекомендовать для клонального микроразмножения винограда на этапе микрочеренкования. The grape variety ‘Kober 5 BB’ (‘Berlandieri x Riparia Kober 5BB’) is one of the main rootstocks used in rootstock-growing farming to obtain grafted seedlings. Currently it is a hot issue to create nurseries for the rootstock grapevine with planting material of the "original" category. This explains the need for research related to optimization of cultivation conditions of the variety ‘Kober 5BB’ to increase the efficiency of mass clonal micropropagation while retaining its genetic homogeny and stability. The aim of the study was to optimize and select nutrient media for clonal micropropagation of the rootstock variety ‘Kober 5 BB’ at the stage of tiraging (micropropagation by cutting). The material of research was the in vitro plants of ‘Kober 5BB’ rootstock variety, free from basic pathogenic infection (according to the test results). The studies were carried out on media: MS; WPM; DKW; PG (control). Gibberellic acid (GA) was used as a growth regulator at concentrations: 0.2; 0.6; 1; 1.4 mg/l in combination with NAA (α-naphthyl acetic acid) 0.05 mg/l. Plants on WPM medium containing NAA-0.05 mg/l in biometric parameters were superior to those grown on PG medium with a similar hormonal composition. According to the results of biometric parameters the studies on optimization the culture medium for accelerating growth processes made it possible to isolate a group of media with a WPM base for propagation of the rootstock variety ‘Kober 5 BB’ at the stage of microcutting. After additional studies with expanded selection, the WPM medium can be recommended for clonal micropropagation of grapes at the stage of microcutting.

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

2021 ◽  
pp. 520-528
Author(s):  
Sergey Makarov ◽  
Sergey Rodin ◽  
Irina Kuznetsova ◽  
Anton Chudetsky ◽  
Svetlana Tsaregradskaya
Keyword(s):  
In Vitro Rooting ◽  
Light Emitting ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Medicinal Value ◽  
American Cranberry ◽  
Food Market ◽  
Spectral Ranges

Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.

scholarly journals Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.)

2021 ◽  
Vol 51 (1) ◽  
pp. 67-76
Author(s):  
Sergey Makarov ◽  
Irina Kuznetsova ◽  
Mikhail Upadyshev ◽  
Sergey Rodin ◽  
Anton Chudetsky
Keyword(s):  
Survival Rate ◽  
Nutrient Medium ◽  
Average Length ◽  
Forest Products ◽  
Hybrid Form ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Total Growth

Introduction. The last decade saw a considerable increase in the demand for European cranberry planting material (Oxyccocus palustris Pers.) among consumers of non-timber forest products. Cranberry possesses high nutritional and medicinal value. Cultivars and hybrids of European cranberry prove extremely productive for plantation growth using the method of clonal micropropagation with revitalized planting material. Study objects and methods. The research featured European cranberry plants of the Dar Kostromy cultivar and its hybrid form 1-15-635. The study focused on the effect of various medications and growth regulators on the biometric profile of European cranberry and its adaptation to non-sterile conditions at all stages of in vivo clonal micropropagation. Results and discussion. During the introduction stage, the highest viability belonged to the explants treated with AgNO3 (95–96%) and Lizoformin 3000 (5%) as the main sterilizing solutions at a 10-min exposure and a 5% solution of Ecosterilizer (1:1) at a 20-min exposure (90–95%). During the micropropagation proper, the number, average length, and total growth of shoots increased as the concentration of cytokinin 2ip in the WPM 1/4 nutrient medium rose from 1.0 to 5.0 mg/L. At the stage of in vitro rooting, the maximal number, average length, and total growth of roots in regenerated plants for both cultivars were observed when Kornerost 5.0 mg/L was added to the WPM 1/4 nutrient medium. At the stage of adaptation to in vivo conditions, Micogel 0.2 mg/L contributed to the highest survival rate (94–100%). Conclusion. During clonal micropropagation in vitro, the biometric profile of European cranberry (Oxyccocus palustris Pers.) and its survival rate under non-sterile conditions in vivo proved to depend on various growth-regulating substances and their concentrations.

scholarly journals Use of growth regulators and experimental LED phytoirradiator in clonal micropropagation of garden strawberry (Fragaria × ananassa, Duchesne ex Weston)

2019 ◽  
Vol 20 (4) ◽  
pp. 324-333 ◽  
Author(s):  
M. G. Markova ◽  
E. N. Somova
Keyword(s):  
Experimental Data ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Fragaria Ananassa ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Combined Use ◽  
The Impact ◽  
Reproduction Factor

The article provides experimental data of 2017-2018 study on the effect of growth regulators and LED phytoirradiator on the proliferation and rooting of promising garden strawberry (Fragaria ananassa) varieties in vitro. Micro-shoots of Korona and Brighton strawberry varieties were taken as the object of the research. Strawberry micro-shoots were cultivated under fluorescent lamps in the control variant. A programmable combined blinking LED phytoirradiator was under study. The combined effect of cytokinin and gibberellic acid by adding them to the Murashige and Skoog nutrient medium, as well as the impact of Siliplant and EcoFus growth regulators on strawberry micropropagation has been studied. It was established that in the cultivation of Korona variety the combined use of Siliplant and EcoFus under illumination with LED phytoirradiator provided an increase in the reproduction factor. The coefficient was 5.0 pcs./explant that was 1.7 times higher than the control (3.0 pcs/explant), the LSD05 1.4 pcs/explant. The maximum reproduction factor of remontant strawberry Brighton variety was obtained in the variant with the use of Siliplant and LED phytoirradiator and amounted to 4.9 pcs./explant (4.2 pcs./explant in the control), the LSD05 was 1.5 pcs./ explant. Regardless of the lighting, the use of RibavExtra in all variants under study increased the rooting rate of the strawberry Korona micro-shoots from 92.8 to 99.1%, the LSD05 6.1%. The use of LED phytoirradiator in comparison with the luminescent one (94.3%) provided a significant increase in the rooting rate of the strawberry Korona micro-shoots to 98.1% regardless of the growth regulators used, the LSD05 3.5%. The combined use of LED phytoirradiator and Ribav-Extra growth regulator in concentrations of 1.0 and 1.5 mg/l resulted in rooting of strawberry Korona micro-shoots up to 100%. Regardless of the growth regulator used, the use of LED phytoirradiator in comparison with the luminescent one (88.9%) provided a significant increase in the rooting rate of the strawberry Brighton micro-shoots to 97.2%, the LSD05 4.6%. The rooting rate of the remontant strawberry Brighton microshoots was 100% in the variant with the use of Ribav-Extra in the concentration of 1.0 mg /l combined with LED phytoirradiator 20 days after transplanting for rooting.

scholarly journals Clonal micropropagation of bog cranberry (Vaccinium oxycoccos L.) in the Udmurt Republic

2021 ◽  
Vol 22 (1) ◽  
pp. 57-66
Author(s):  
E. N. Cheremnykh ◽  
T. G. Lekontseva ◽  
A. V. Khudyakova ◽  
A. V. Fedorov
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Root Formation ◽  
Sphagnum Moss ◽  
Multiplication Factor ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Udmurt Republic

The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.

scholarly journals Producing Planting Material of Rubus arcticus L. by Clonal Micropropagation

2021 ◽  
pp. 89-99
Author(s):  
Sergey S. Makarov ◽  
◽  
Galina V. Tyak ◽  
Anton I. Chudetsky ◽  
Irina B. Kuznetsova ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Experimental Studies ◽  
Forest Products ◽  
The Arctic ◽  
High Yield ◽  
Nontimber Forest Products ◽  
Effect Analysis ◽  
Clonal Micropropagation ◽  
Planting Material

The article presents the results of experimental studies on various propagation methods of the Arctic bramble in the Kostroma region. Industrial cultivation of forest berry plantations is a possible effective solution to the problem of low profitability of using nontimber forest products, reduction of the wild berries resources and their productivity and quality, and reclamation of cutover peatlands. It is advisable to use high-yield varietal planting material to create such plantations. Special attention is paid to propagation and production of healthy planting material using culture of plant cells and tissues. Data on sterilization of explants when introduced in vitro are given. The highest efficiency of sterilization was observed when using a chlorine-free eco-sterilizer (the plant survival rate on the MS nutrient medium was 90–93 %). The effect analysis of the passage number of regenerated plants on the multiplication factor of the Arctic bramble varieties was carried out. The optimal concentrations of cytokinins at the stage of micropropagation are shown. The largest number of the Arctic bramble roots was observed when adding 1.0 mg/L of Indole-3-butyric acid (IBA) and 0.5 mg/L of Ecogel to the nutrient medium. The technological and agrotechnical operations performed during the cultivation of planting material of forest berry plantations are considered. Data on the coefficients of vegetative propagation of plants and their resistance to diseases, yielding capacity, and recultivation of cutover peatlands are given. The best planting material of the Arctic bramble are ball-rooted seedlings. Sawdust and sphagnum were used in the cultivation of this berry on the peatland. The economic efficiency of its cultivation with the method of clonal micropropagation was 358.2 %. For citation: Makarov S.S., Tyak G.V., Kuznetsova I.B., Chudetsky A.I., Tsaregradskaya S.Yu. Producing Planting Material of Rubus arcticus L. by Clonal Micropropagation. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 6, pp. 89–99. DOI: 10.37482/0536-1036-2021-6-89-99

scholarly journals MICROPROPAGATION STUDIES AND ANTIOXIDANT ANALYSIS OF THE ENDANGERED PLANTS OF BULGARIAN YELLOW GENTIAN (Gentiana lutea L.)

2019 ◽  
Vol 18 (3) ◽  
pp. 71-78
Author(s):  
Maria I. Petrova ◽  
Ely G. Zayova ◽  
Lyudmila I. Dimitrova ◽  
Maria P. Geneva ◽  
Kamelia D. Miladinova-Georgieva
Keyword(s):  
Growth Regulators ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Guaiacol Peroxidase ◽  
Nutrient Media ◽  
Shoot Height ◽  
Gentiana Lutea ◽  
Antioxidant Metabolites ◽  
Rare And Endangered

In order to develop an efficient micropropagation system, it is essential to establish the appropriate concentration of growth regulators for seed germination, shoot formation and rooting. Nodal segments from in vitro obtained seedlings of Gentiana lutea L. were cultured in vitro in Murashige and Skoog’s medium supplemented with BAP, Thidiazuron and Zeatin (0.5, 1.0 and 2.0 mg L−1). A maximum number of shoots with the highest height was recorded at 2.0 mg L−1 BAP. For further optimization of the process, we used nutrient media containing BAP and Zeatin with a combination of low concentration of Indoleacetic acid. MS medium containing 2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA resulted in maximum numbers of shoots 94.3) with shoot height 2.5 cm. The multiple plants were successfully ex vitro acclimatized with 65% survival. The presence of growth regulators (2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA) in the nutrient media resulted in an effective antioxidant activity in G. Lutea determined by the low molecular antioxidant metabolites such as phenols and flavonoids and activities of antioxidant enzymes – catalase, ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase. The described protocol allows the establishment of numerous micropropaged plants of rare and endangered G. lutea.

scholarly journals Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona

2016 ◽  
Vol 8 (2) ◽  
pp. 535-540
Author(s):  
Priyadarshani P. Mohapatra ◽  
V.K. Batra ◽  
Subhash Kajla ◽  
Anil K. Poonia ◽  
N. Manoj Kumar
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Basal Medium ◽  
Potato Cultivar ◽  
Shoot Tips ◽  
Scale Production ◽  
Survival Percentage ◽  
Planting Material ◽  
Micro Propagation

In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.

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