Effects of mulberry (Morus alba L.) Leaf extracts on growth, immune response, and antioxidant functions in nile tilapia (Oreochromis niloticus)

Abstract This study evaluates how white mulberry (Morus alba L.) leaf extracts affect the growth, antioxidant activity, and immune response in Nile tilapia Oreochromis niloticus. Mulberry leaf extracts were obtained through aqueous extraction (AE) and ethanol extraction (EE). Powder of mulberry leaf (PML) was added directly to feed and compared with the effects of feeds supplemented with the different extracts. Fish were divided into eight groups for an 8-week feeding trial where they were fed the basal diet or supplementation with 10% PML, 10% AE, 20% AE, 40% AE, 10% EE, 20% EE, or 40% EE. The inclusion of mulberry leaf extract obtained with either method showed better effects on fish growth performance, antioxidant activities and acid phosphatase activity (ACP) in serum, immune cytokine expression, and intestinal morphology as compared with controls or fish fed the 10% PML diet. The specific growth rate was significantly higher in the 10% AE, 10% EE, and 20% EE groups compared with all other groups (P<0.05). Catalase activity was significantly greater in most groups fed an extract, and in the 10% PML group, when compared with controls. Similarly, ACP, interleukin (IL)-1, and IL-2 expression was significantly increased in groups fed an extract, and in the 10% PML group, when compared with controls (P<0.05). IL-1, IL-2, IL-10, and Toll-like receptor 2 expression was significantly greater in the 10% EE group than in the 10% PML and 10% AE groups (P<0.05). Villus length in the middle intestine was significantly increased in the 10% AE and 10% EE groups compared with controls and the 10% PML group (P<0.05). Thus, 10% mulberry leaf ethanol extract added to feed is recommended for enhancing the growth rate and health of cultured Nile tilapia.

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The use of Sponge Crude Extract to Increase Growth and Immune Response of Nile Tilapia Oreochromis niloticus

JURNAL ILMIAH PLATAX ◽

10.35800/jip.7.1.2019.23228 ◽

2019 ◽

Vol 7 (1) ◽

pp. 256

Author(s):

Merlyn D. Manurung ◽

Reiny A. Tumbol ◽

Henneke D. Pangkey ◽

Deiske A. Sumilat ◽

Remy E. P. Mangindaan

Keyword(s):

Immune Response ◽

Crude Extract ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Fish Growth ◽

Specific Immune Response ◽

Good Effect ◽

Fish Feed ◽

Nile Tilapia Oreochromis Niloticus ◽

Rotary Vacuum

This study aims to examine the effect of sponge crude extract addition to the fish feed on growth and non-specific immune response of nile tilapia (O. niloticus) and determine the most effective dose of the extract in raising the growth and the non-specific immune response of nile tilapia (O. niloticus). The sponge sample was macerated and then evaporated using a rotary vacuum evaporator until the sample became a paste and mixed with feed. The fish were fed for 14 days respectively as much as 5% of body weight per day with feeding frequency of two times a day. The results showed that addition of sponge crude extract to the fish feed gave good effect on the growth and the non-specific immune response of nile tilapia (O. niloticus) with best dose of 40 g / kg feed.Keywords: Sponge extract, fish immune response, fish growth, immune response, immunostimulant. ABSTRAKPenelitian ini bertujuan untuk menguji pengaruh penambahan ekstrak kasar spons pada pakan ikan terhadap pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus) serta menentukan dosis ekstrak kasar spons yang paling efektif dalam meningkatkan pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus). Sampel spons dimaserasi kemudian dievaporasi menggunakan rotary vacuum evaporator sehingga diperoleh ekstrak kasar berbentuk pasta yang kemudian dicampurkan pada pakan. Ikan uji diberi pakan perlakuan 5% dari bobot biomassa selama 14 hari dengan frekuensi pemberian pakan dua kali sehari. Hasil penelitian menunjukan bahwa penambahan ekstrak kasar spons pada pakan ikan memberikan pengaruh yang baik terhadap pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus), yang mana dosis yang terbaik adalah 40 gr / Kg pakan.Kata Kunci : Ekstrak spons, respon imun ikan, pertumbuhan ikan, respon imun, imunostimulan.

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Psidium Guajava Leaf Extracts Fed to Mono-sex Nile Tilapia Oreochromis Niloticus Enhance Immune Response Against Pseudomonas fluorescens

European Journal of Clinical and Biomedical Sciences ◽

10.11648/j.ejcbs.20170301.16 ◽

2017 ◽

Vol 3 (1) ◽

pp. 34 ◽

Cited By ~ 1

Author(s):

Jannatul Ferdous

Keyword(s):

Immune Response ◽

Pseudomonas Fluorescens ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Psidium Guajava ◽

Leaf Extracts

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Identification and functional characterization of CD154 in T cell-dependent immune response in Nile tilapia (Oreochromis niloticus)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2021.01.009 ◽

2021 ◽

Vol 111 ◽

pp. 102-110

Author(s):

Bingxi Li ◽

Yuan Li ◽

Siwei Wu ◽

Yanjian Yang ◽

Shengli Fu ◽

...

Keyword(s):

Immune Response ◽

T Cell ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Functional Characterization ◽

Nile Tilapia Oreochromis Niloticus

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Exploring the Multimodal Role of Yucca schidigera Extract in Protection against Chronic Ammonia Exposure Targeting: Growth, Metabolic, Stress and Inflammatory Responses in Nile Tilapia (Oreochromis niloticus L.)

Animals ◽

10.3390/ani11072072 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2072

Author(s):

Zizy I. Elbialy ◽

Abdallah S. Salah ◽

Ahmed Elsheshtawy ◽

Merna Rizk ◽

Muyassar H. Abualreesh ◽

...

Keyword(s):

Nile Tilapia ◽

Inflammatory Responses ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Metabolic Stress ◽

Fish Growth ◽

Control Group ◽

Feed Conversion ◽

Final Body Weight ◽

Yucca Schidigera

Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its control, ammonia intoxication remains one of the most critical issues to overcome. In this study, we explored the modulatory effect and potential mechanism by which Yucca schidigera extract (YSE) can ameliorate ammonia intoxication-induced adverse effects on tilapia health and metabolism. A total number of 120 Nile tilapia were evenly assigned into four groups with three replicates each. The first group served as normal control group; the second group was exposed to ammonia alone from the beginning of the experiment and for four weeks. The third group was supplied with YSE in water at a dose of 8 mg/L and exposed to ammonia. The fourth group was supplied with YSE only in water at a dose of 8 mg/L. YSE supplementation succeeded in improving water quality by reducing pH and ammonia levels. Moreover, YSE supplementation markedly alleviated chronic ammonia-induced adverse impacts on fish growth by increasing the final body weight (FBW), specific growth rate (SGR), feed intake and protein efficiency ratio (PER) while reducing the feed conversion ratio (FCR) via improvements in food intake, elevation of hepatic insulin-like growth factor (ILGF-1) and suppression of myostatin (MSTN) expression levels with the restoration of lipid reserves and the activation of lipogenic potential in adipose tissue as demonstrated by changes in the circulating metabolite levels. In addition, the levels of hepato-renal injury biomarkers were restored, hepatic lipid peroxidation was inhibited and the levels of hepatic antioxidant biomarkers were enhanced. Therefore, the current study suggests that YSE supplementation exerted an ameliorative role against chronic ammonia-induced oxidative stress and toxic effects due to its free radical-scavenging potential, potent antioxidant activities and anti-inflammatory effects.

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