scholarly journals Effects of copper and cadmium on physiology and antifouling defense of the marine macroalga Ulva reticulata

2021 ◽  
Vol 50 (2) ◽  
pp. 184-197
Author(s):  
Wejdan Ahmed Al-Khaldi ◽  
Lafi Al Solami ◽  
Sathianeson Satheesh

Abstract Heavy metals are major stressors for benthic macroalgal communities in marine ecosystems. In this study, the effects of copper and cadmium on some physiological parameters along with antifouling defense of the marine macroalga Ulva reticulata were assessed under laboratory conditions. Macroalgal samples were treated with three concentrations (1 mg l−1, 3 mg l−1 and 5 mg l−1) of copper and cadmium for 2 and 7 days. After treatment, algal samples were analyzed for chlorophyll-a, carotenoid, total polyphenol and total antioxidant capacity. Also, algal extracts were tested against biofilm-forming bacteria strains to understand differences in antifouling activity. The results indicated that exposure of U. reticulata to copper and cadmium, on the one hand, induced protective mechanisms such as total phenol production and antioxidant capacity against metal stress and, on the other hand, reduced photosynthesis. While the extract obtained from control algal samples showed a strong inhibitory effect on the growth of biofilm-forming bacteria, treatment with heavy metals resulted in reduced antibiofilm activity. In general, the results revealed that exposure of macroalgae to heavy metals can affect antifouling defense traits in addition to changes in photosynthetic pigment content.

2011 ◽  
Vol 396-398 ◽  
pp. 52-55 ◽  
Author(s):  
Ming Xiong Tan ◽  
Yan Cheng Liu ◽  
Xu Jian Luo ◽  
Dong Qing Li

The total alkaloids (TAE) were obtained from the fruits of Evodia rutaecarpa (Juss.) Benth. The antioxidant activities of TAE towards the inhibitory effect on 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical, total antioxidant capacity, and inhibition on lipid peroxidation were investigated. TAE was found that the total antioxidant capacity and inhibitory lipid peroxidation are superior to synthetic antioxidant 2, 6-di-ter-butyl-4-methylphenol (BHT), but scavenging activity on DPPH radical is lower than that of BHT at the same condition.


2012 ◽  
Vol 610-613 ◽  
pp. 467-471
Author(s):  
Tian Rong Guo

Photosynthetic pigment content and photosynthetic activity, as well as the antioxidant capacity were estimated in barley seedlings after 10 or 20 d supplies of Al and Cd individual or combined in nutrient solution. The results showed that stress exposure strongly decreased photosynthetic pigment content and photosynthetic activity, as well as induced oxidative damage as evidenced by increased the activities of peroxidase (POD) and superoxide dismutase (SOD), and malonaldehyde (MDA) content in barley leaves. The most obvious response was found in plants exposed to higher levels of Cd (10 μmol/L) or Al (90 μmol/L) in the nutrient medium. Binary metal combinations generally aggravated photosynthesis reduction and induced more serious oxidative stress as compared with Al or Cd single treatments, and the most deleterious effects were observed in the combination of 90 μmol/L Al+5 μmol/L Cd.


Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1821
Author(s):  
Alica Bobková ◽  
Alžbeta Demianová ◽  
Ľubomír Belej ◽  
Ľuboš Harangozo ◽  
Marek Bobko ◽  
...  

Tea (Camellia sinensis) is widely sought for beverages worldwide. Heavy metals are often the main aims of the survey of teas, given that the use of agricultural fertilization is very frequent. Some of these may affect the content of bioactive compounds. Therefore, in this study, we analyzed fermented and non-fermented teas of a single plant origin from Japan, Nepal, Korea, and China, and described mutual correlations and changes in the total antioxidant capacity (TAC), and the content of polyphenols (TPC), caffeine, and heavy metals in tea leaves, in relation to the origin and fermentation process. Using UV-VIS spectrophotometry and HPLC-DAD, we determined variations in bioactive compounds’ content in relation to the fermentation process and origin and observed negative correlations between TAC and TPC. Heavy metal content followed this order: Mn > Fe > Cu > Zn > Ni > Cr > Pb > Co > Cd > Hg. Given the homogenous content of these elements in relation to fermentation, this paper also describes the possibility of using heavy metals as determinants of geographical origin. Linear Discriminant Analysis showed an accuracy of 75% for Ni, Co, Cd, Hg, and Pb, explaining 95.19% of the variability between geographical regions.


Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Ingrid Žitňanová ◽  
Silvia Ranostajová ◽  
Hana Sobotová ◽  
Denisa Demelová ◽  
Ivan Pecháň ◽  
...  

AbstractRecent studies have demonstrated that dietary plants are rich source of antioxidants and can contribute to the protection from age-related diseases. The aim of our study was to determine the total antioxidant capacity of extracts from different kinds of fruits and vegetables, and to examine their inhibitory effect on the oxidative damage to proteins in vitro. For determination of antioxidant capacity we used two direct methods. Among the food materials chosen for the present study, blueberries and red beets gave the maximum antioxidant activity. The lowest activity was determined in pears and green beans. Some extracts were more active in one method, while their activity was lower using the other method. To investigate inhibitory effects of fruits and vegetables extracts on the oxidative damage to proteins in vitro, we induced the oxidative damage to plasma proteins by sodium hypochlorite leading to formation of carbonyl compounds detected by spectrophotometric method. All extracts of fruits and vegetables showed inhibitory activity on the oxidative damage to proteins with raspberries and leek as most effective. Results of this study will be useful as an aid for dietary choices to increase antioxidant intake and will allow the investigation of the relation between dietary antioxidants and oxidative stress-induced diseases.


Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.


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