Epidemiology of African Swine Fever in Poland since the detection of the first case

2014 ◽  
Vol 17 (4) ◽  
pp. 665-672 ◽  
Author(s):  
Z. Pejsak ◽  
M. Truszczyński ◽  
K. Niemczuk ◽  
E. Kozak ◽  
I. Markowska-Daniel
Keyword(s):  
Wild Boar ◽  
African Swine Fever ◽  
Reference Laboratory ◽  
Surveillance Programme ◽  
National Reference ◽  
Veterinary Research ◽  
First Case ◽  
The Russian Federation ◽  
National Veterinary Research Institute ◽  
Backyard Pigs

Abstract The purpose of this paper is to provide characteristics of the spread of African Swine Fever (ASF) in Poland from February to August, 2014. The samples from dead wild boar and domestic pigs were submitted to the National Veterinary Research Institute, National Reference Laboratory for ASF in Pulawy, Poland, for testing by PCR and ELISA methods. In the studied period, fourteen cases of ASF in wild boar and two outbreaks in backyard pigs were confirmed. In addition to the results of laboratory tests performed in 2014, the article describes the ASF surveillance programme in wild boar and pigs in Poland carried out in 2011-2013. The spread of ASF in Poland is compared with the epidemiological situation in Lithuania, Latvia, Belarus and the Russian Federation.

Molecular characterisation of Mycobacterium caprae strains isolated in Poland

2017 ◽  
Vol 182 (10) ◽  
pp. 292-292 ◽  
Author(s):  
Monika Krajewska-Wędzina ◽  
Monika Kozińska ◽  
Blanka Orłowska ◽  
Marcin Weiner ◽  
Krzysztof Szulowski ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Genotypic Diversity ◽  
Reference Laboratory ◽  
Molecular Characterisation ◽  
Wild Animals ◽  
Veterinary Research ◽  
Mycobacterium Caprae ◽  
Explicit Mention ◽  
Cattle Herds ◽  
National Veterinary Research Institute

Bovine tuberculosis (bovine TB, bTB) is caused by bovine bacilli: Mycobacterium bovis and M caprae. The studies conducted in Poland, in the National Bovine Tuberculosis Reference Laboratory in the Department of Microbiology of the National Veterinary Research Institute in Pulawy, show that animal tuberculosis in Poland is also caused by M caprae. We here describe the identification and genotypic assessment of 52 isolates of M caprae obtained from Polish cattle and wild animals over the last five years. We show that strains isolated from bison have significant genotypic diversity and are distinct compared with the genotypes of strains isolated from cattle. Similarly, isolates from cattle herds can be highly genotypically variable. Formal designation of the members of the Mycobacterium tuberculosis complex is controversial in Poland; there is a gap in veterinary legislation with regard to bTB and no explicit mention of M caprae causing tuberculosis in animal.

scholarly journals The African Swine Fever Epidemic in Wild Boar (Sus scrofa) in Lithuania (2014–2018)

2020 ◽  
Vol 7 (1) ◽  
pp. 15 ◽  
Author(s):  
Petras Mačiulskis ◽  
Marius Masiulis ◽  
Gediminas Pridotkas ◽  
Jūratė Buitkuvienė ◽  
Vaclovas Jurgelevičius ◽  
...  
Keyword(s):  
Wild Boar ◽  
Population Decline ◽  
African Swine Fever ◽  
Control Measures ◽  
Disease Spread ◽  
Wild Boar Population ◽  
Baltic States ◽  
Long Distance ◽  
First Case ◽  
The Baltic

In January 2014 the first case of African swine fever (ASF) in wild boar of the Baltic States was reported from Lithuania. It has been the first occurrence of the disease in Eastern EU member states. Since then, the disease spread further affecting not only the Baltic States and Poland but also south-eastern Europe, the Czech Republic and Belgium. The spreading pattern of ASF with its long-distance spread of several hundreds of kilometers on the one hand and the endemic situation in wild boar on the other is far from being understood. By analyzing data of ASF cases in wild boar along with implemented control measures in Lithuania from 2014–2018 this study aims to contribute to a better understanding of the disease. In brief, despite huge efforts to eradicate ASF, the disease is now endemic in the Lithuanian wild boar population. About 86% of Lithuanian’s territory is affected and over 3225 ASF cases in wild boar have been notified since 2014. The ASF epidemic led to a considerable decline in wild boar hunting bags. Intensified hunting might have reduced the wild boar population but this effect cannot be differentiated from the population decline caused by the disease itself. However, for ASF detection sampling of wild boar found dead supported by financial incentives turned out to be one of the most effective tools.

scholarly journals The Spillover of African Swine Fever in Western Poland Revealed Its Estimated Origin on the Basis of O174L, K145R, MGF 505-5R and IGR I73R/I329L Genomic Sequences

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1094 ◽  
Author(s):  
Natalia Mazur-Panasiuk ◽  
Marek Walczak ◽  
Małgorzata Juszkiewicz ◽  
Grzegorz Woźniakowski
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Wild Boar ◽  
Tandem Repeats ◽  
African Swine Fever ◽  
Whole Genome Sequence ◽  
Reference Sequence ◽  
Nucleotide Sequencing ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
First Case

The African swine fever epidemic occurred in Poland at the beginning of 2014 and, up to date, the disease has been spreading mainly in the eastern part of the country. Unexpectedly, in November 2019 an infected wild boar case was confirmed in Lubuskie voivodship in western Poland. During the following weeks, several dozen African swine fever virus (ASFV)-positive animals were notified in the neighboring area, causing severe concern regarding further spread of the disease to the mostly pig-dense region in Poland, namely, Wielkopolskie voivodship. Moreover, almost a year after, several infected wild boar cases were confirmed for the first time in Germany, just beyond the Polish border, sending out a shock wave through the global pig market. The whole genome sequence of ASFV, isolated from the first case of ASF in western Poland, and three selected viruses from other affected areas, revealed the tandem repeat and single nucleotide polymorphism (SNP) variations in reference to the Georgia 2007/1 strain. These data, supported by the conventional sequencing of selected genomic regions from a total of 154 virus samples isolated between 2017 and 2020 in Poland, shed a new light on pathogen epidemiology. The sequence variations within the O174L gene detected in this study showed that cases identified in western Poland might be originating from the so-called southern Warsaw cluster. Moreover, the viruses originating from the northern Warsaw cluster do not possess single nucleotide polymorphism (SNP) mutations within the K145R and MGF 505-5R genes, which are specific to all of the other Polish ASFV strains. These results led to a conclusion of their distinct origin. Supporting these results, the nucleotide sequencing of I73R/I329L intergenic region revealed its new, previously undescribed variant, called IGR IV, with an additional three tandem repeats of 10 nucleotides in comparison to the reference sequence of the Georgia 2007/1 strain.

scholarly journals Molecular Characterization of African Swine Fever Virus Isolates in Estonia in 2014–2019

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 582 ◽  
Author(s):  
Annika Vilem ◽  
Imbi Nurmoja ◽  
Tarmo Niine ◽  
Taavi Riit ◽  
Raquel Nieto ◽  
...  
Keyword(s):  
Wild Boar ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Fever Virus ◽  
Limited Area ◽  
First Case ◽  
Central Variable Region ◽  
Genotype Ii

After the extensive spread of the African swine fever virus (ASFV) genotype II in Eastern Europe, the first case of African swine fever (ASF) in Estonia was diagnosed in September 2014. By the end of 2019, 3971 ASFV-positive wild boars were found, and 27 domestic pig outbreaks were reported. A selection of ASFV isolates from wild boar and domestic pigs (during the period of September 2014–2019) was molecularly characterized using standardized genotyping procedures. One of the proven markers to characterize this virus is the central variable region (CVR) within the B602L gene. In summer 2015, a new ASFV genotype II CVR variant 2 (GII-CVR2) was confirmed in Estonia. The results suggest that the GII-CVR2 variant was only confirmed in wild boar from a limited area in southern Estonia in 2015 and 2016. In addition to GII-CVR2, a single nucleotide polymorphism (SNP) that resulted in amino acid change was identified within the genotype II CVR variant 1 (GII-CVR1). The GII-CVR1/SNP1 strain was isolated in Estonia in November 2016. Additional GII-CVR1/SNP1 cases were confirmed in two neighbouring counties, as well as in one outbreak farm in June 2017. Based on the available data, no GII-CVR2 and GII-CVR1/SNP1 have been reported by other affected European countries. The spread of variant strains in Estonia has been limited over time, and restricted to a relatively small area.

scholarly journals Experience in African swine fever control in the Russian Federation and its value for the other countries

2020 ◽  
pp. 38-43
Author(s):  
K. N. Gruzdev ◽  
A. K. Karaulov ◽  
A. S. Igolkin
Keyword(s):  
Wild Boar ◽  
Russian Federation ◽  
Population Control ◽  
African Swine Fever ◽  
Production Chain ◽  
The Russian Federation ◽  
Basic Characteristics ◽  
Management Approaches ◽  
High Level ◽  
First Time

Basic characteristics of African swine fever, being one of the most significant transboundary infections with a devastating potential for pig production, are presented in the paper. It occurred in Georgia for the first time in 2007 and spread among domestic pigs and wild boar in the European part of the Russian Federation. After that, ASF virus was detected in Belarus, Ukraine and since 2014, the disease has been reported in the European countries. Having spread in Europe, African swine fever became an epizooty, caused by ASFV genotype II and exhibiting a deteriorating trend. Modern ASF infected areas in the Russian Federation, Europe, China and Asia are described. Currently the disease is the most serious epidemiological problem due to huge losses (high mortality among susceptible animals); ability to occur and spread in the most unexpected parts of the world and absence of specific prevention tools and means. Russia has developed a high level of expertise in the disease prevention, control and eradication. A scientifically justified set of measures to prevent and eradicate ASF, which has proved its effectiveness in the disease control, was developed and introduced. The core of this set is formed by biosafety assurance along the whole production chain at the establishments of any type of ownership involved into breeding, keeping, slaughter, processing, storage, movement and marketing of live pigs and pig products; by wild boar population control and improvement of waste management approaches. Other ASF infected countries can benefit from the Russian Federation’s experience in ASF control by adapting it to the concrete region with due regard to local social and economic conditions.

scholarly journals First Occurence of African Swine Fever in Serbia

2019 ◽  
Vol 69 (4) ◽  
pp. 443-449
Author(s):  
Vesna Milićević ◽  
Branislav Kureljušić ◽  
Jelena Maksimović Zorić ◽  
Božidar Savić ◽  
Slobodan Stanojević ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Surveillance Program ◽  
Reference Laboratory ◽  
First Case ◽  
Record Data ◽  
Gross Lesions ◽  
Virus Isolates ◽  
The Eu

Abstract Until July 30th, 2019 when the first case of African swine fever (ASF) was confirmed, Serbia was a country free from ASF. After the owner reported atypical illness and death of a sow, the local veterinarian submitted the organ samples to the National Reference Laboratory for Classical Swine Fever (CSF) and African Swine Fever within the Institute of Veterinary Medicine of Serbia in Belgrade. Observed gross lesions included splenomegaly, serous edema of the wall of the gallbladder and hemorrhages in the enlarged portal lymph nodes, petechial hemorrhages on the kidney and epicardium, and petechial and echymotic hemorrhages on the mucosa of the urinary bladder. Results of real-time PCR confirmed that the cause of illness and death of the swine was African swine fever virus. The samples were sent for confirmation to the EU Reference Laboratory where it was confirmed that Serbian domestic pig virus isolates based on p72 belong to genotype II. In total, 270 pigs from 18 affected holdings were killed in the infected zones. According to the on-record data, mortality was 6.89%, whereas lethality reached 64.5%. Currently, an extensive surveillance program is being conducted, aiming to force passive surveillance. ASF in wild boar has not been confirmed so far.

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